Restriction map for
pFN21AB6384
(Circular) (Six-base) MAPSORT of: pFN21AB6384.seq Check: 5143 from: 1 to: 5605
pFN21AB6384 5605 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2804 2804
Size: 5605
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3415 278
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2481 2948 1937
Size: 544 467 4594
Fragments arranged by size:
4594 544 467
Acc65I G'GTAC_C
Cuts at: 2926 2926
Size: 5605
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2211 3668 5052 5320 1632
Size: 579 1457 1384 268 1917
Fragments arranged by size:
1917 1457 1384 579 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 4504 5481 1203
Size: 3301 977 1327
Fragments arranged by size:
3301 1327 977
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 4334 4334
Size: 5605
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5605
AlwNI CAG_nnn'CTG
Cuts at: 3348 4920 3348
Size: 1572 4033
ApaI G_GGCC'C
Cuts at: 2832 2832
Size: 5605
ApaLI G'TGCA_C
Cuts at: 3662 4818 3662
Size: 1156 4449
ApoI r'AATT_y
Cuts at: 1493 2914 3140 3292 5401 1493
Size: 1421 226 152 2109 1697
Fragments arranged by size:
2109 1697 1421 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2026 2930 1870
Size: 73 83 904 4545
Fragments arranged by size:
4545 904 83 73
BamHI G'GATC_C
Cuts at: 2935 3302 2935
Size: 367 5238
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2926 4381 618
Size: 325 217 627 1139 1455 1842
Fragments arranged by size:
1842 1455 1139 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2832 2924 1391
Size: 1441 92 4072
Fragments arranged by size:
4072 1441 92
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2316 2517 928
Size: 971 417 201 4016
Fragments arranged by size:
4016 971 417 201
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3796 3830 1903
Size: 34 1859 34 3678
Fragments arranged by size:
3678 1859 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3497 4707 3497
Size: 1210 4395
BclI T'GATC_A
Cuts at: 1548 2395 1548
Size: 847 4758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2617 4216 136
Size: 107 122 71 631 605 945 1599 1525
Fragments arranged by size:
1599 1525 945 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2520 5600 2520
Size: 3080 2525
BlpI GC'TnA_GC
Cuts at: 3022 3022
Size: 5605
Bme1580I G_kGCm'C
Cuts at: 1790 2832 3666 4822 1790
Size: 1042 834 1156 2573
Fragments arranged by size:
2573 1156 1042 834
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2282 2314 2608
Size: 416 53 83 399 24 865 32 294
Cuts at: 2608 3336 4294 442
Size: 728 958 1753
Fragments arranged by size:
1753 958 865 728 416 399 294 83
53 32 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2387 4265 1239
Size: 836 312 1878 2579
Fragments arranged by size:
2579 1878 836 312
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3730 4595 4893 3730
Size: 865 298 4442
Fragments arranged by size:
4442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2711 4268 882
Size: 617 1212 1557 2219
Fragments arranged by size:
2219 1557 1212 617
BsaAI yAC'GTr
Cuts at: 493 1204 3396 493
Size: 711 2192 2702
Fragments arranged by size:
2702 2192 711
BsaBI GATnn'nnATC
Cuts at: 2780 5599 2780
Size: 2819 2786
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3412 3794 4429 275
Size: 53 83 186 936 1879 382 635 1451
Fragments arranged by size:
1879 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2843 4038 4436 4710 4857 5268 2843
Size: 1195 398 274 147 411 3180
Fragments arranged by size:
3180 1195 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2006 2036 1178
Size: 30 165 30 603 30 4747
Fragments arranged by size:
4747 603 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2027 2866 1186
Size: 838 3 839 3925
Fragments arranged by size:
3925 839 838 3
BseYI C'CCAG_C
Cuts at: 2032 2747 4808 5304 2032
Size: 715 2061 496 2333
Fragments arranged by size:
2333 2061 715 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2825 1163
Size: 573 1089 3943
Fragments arranged by size:
3943 1089 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3055 3816 4844 1540
Size: 275 186 1054 761 1028 2301
Fragments arranged by size:
2301 1054 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2262 3666 3751 4822 2262
Size: 1404 85 1071 3045
Fragments arranged by size:
3045 1404 1071 85
BsmI GAATG_Cn'
Cuts at: 3116 3209 3116
Size: 93 5512
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2262 2832 2924 3666 3751 4822 1391
Size: 399 472 570 92 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 570 472 399 92 85
BspEI T'CCGG_A
Cuts at: 5268 5268
Size: 5605
BspHI T'CATG_A
Cuts at: 3494 5224 3494
Size: 1730 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2712 2804 2960 844
Size: 1868 92 156 3489
Fragments arranged by size:
3489 1868 156 92
BsrBI CCG'CTC
Cuts at: 1394 1823 3492 1394
Size: 429 1669 3507
Fragments arranged by size:
3507 1669 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2461 2669 4100 4274 65
Size: 1183 1213 208 1431 174 1396
Fragments arranged by size:
1431 1396 1213 1183 208 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4249 4436 1700
Size: 110 2439 187 2869
Fragments arranged by size:
2869 2439 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5605
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5605
BssSI C'ACGA_G
Cuts at: 3665 4677 3665
Size: 1012 4593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5605
BstBI TT'CG_AA
Cuts at: 4409 4409
Size: 5605
BstEII G'GTnAC_C
Cuts at: 1184 2113 1184
Size: 929 4676
BstYI r'GATC_y
Cuts at: 1972 2520 2743 2935 3302 3687 3704 5156 5242
Size: 548 223 192 367 385 17 1452 86
Cuts at: 5242 5254 5600 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 548 385 367 346 223 192
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2833 2833
Size: 5605
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4114
Fragments arranged by size:
4114 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4866
Fragments arranged by size:
4866 683 56
BtsI GCAGTG_nn'
Cuts at: 2434 2467 3121 3915 3935 2434
Size: 33 654 794 20 4104
Fragments arranged by size:
4104 794 654 33 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2443 2478 529
Size: 35 1879 35 3656
Fragments arranged by size:
3656 1879 35 35
DraI TTT'AAA
Cuts at: 2909 2909
Size: 5605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2844 4612 809
Size: 507 1528 1768 1802
Fragments arranged by size:
1802 1768 1528 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2818 3052 3941
Size: 54 1425 50 275 18 988 234 889
Cuts at: 3941 5570 8
Size: 1629 43
Fragments arranged by size:
1629 1425 988 889 275 234 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3052 1537
Size: 275 1240 4090
Fragments arranged by size:
4090 1240 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2709 3535 1353
Size: 1356 826 3423
Fragments arranged by size:
3423 1356 826
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4192 4566 4712 387
Size: 1064 138 2603 374 146 1280
Fragments arranged by size:
2603 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2211 2387 3668 4265 5052 5320
Size: 393 443 136 176 1281 597 787 268
Cuts at: 5320 1239
Size: 1524
Fragments arranged by size:
1524 1281 787 597 443 393 268 176
136
EcoO109I rG'GnC_Cy
Cuts at: 2798 2828 2829 2837 2798
Size: 30 1 8 5566
Fragments arranged by size:
5566 30 8 1
EcoRI G'AATT_C
Cuts at: 2914 3292 2914
Size: 378 5227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2431 2492 2431
Size: 61 5544
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 4752 1678
Size: 3074 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2006 2035 2038 4246
Size: 32 135 32 431 3 29 3 2208
Cuts at: 4246 4278 4320 4352 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2208 431 135 42 32 32 32
32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2949 3195 669
Size: 988 281 1011 246 3079
Fragments arranged by size:
3079 1011 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5605
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KpnI G_GTAC'C
Cuts at: 2930 2930
Size: 5605
MfeI C'AATT_G
Cuts at: 3204 3204
Size: 5605
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4719 4903 1305
Size: 3414 184 2007
Fragments arranged by size:
3414 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2820 10
Size: 54 1425 343 988 2795
Fragments arranged by size:
2795 1425 988 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3563 3922 4081 518
Size: 648 2397 359 159 2042
Fragments arranged by size:
2397 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3021 3698 4846 5091 1606
Size: 1415 677 1148 245 2120
Fragments arranged by size:
2120 1415 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4114
Fragments arranged by size:
4114 939 552
NdeI CA'TA_TG
Cuts at: 387 2148 387
Size: 1761 3844
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2677 2963 4508 1132
Size: 97 1448 286 1545 2229
Fragments arranged by size:
2229 1545 1448 286 97
PasI CC'CwG_GG
Cuts at: 1348 2068 1348
Size: 720 4885
PciI A'CATG_T
Cuts at: 4504 4504
Size: 5605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2664 1108
Size: 1556 4049
PfoI T'CCnGG_A
Cuts at: 2529 2529
Size: 5605
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2510 2542 3674 3706 5187 5219 2510
Size: 32 1132 32 1481 32 2896
Fragments arranged by size:
2896 1481 1132 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2837 2837
Size: 5605
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 3175 3175
Size: 5605
PspOMI G'GGCC_C
Cuts at: 2828 2828
Size: 5605
PstI C_TGCA'G
Cuts at: 830 2042 2815 2957 830
Size: 1212 773 142 3478
Fragments arranged by size:
3478 1212 773 142
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
SalI G'TCGA_C
Cuts at: 1936 2947 1936
Size: 1011 4594
SbfI CC_TGCA'GG
Cuts at: 2815 2957 2815
Size: 142 5463
ScaI AGT'ACT
Cuts at: 3853 5565 3853
Size: 1712 3893
SfcI C'TryA_G
Cuts at: 826 1045 2038 2811 2953 4088 4769 4960 826
Size: 219 993 773 142 1135 681 191 1471
Fragments arranged by size:
1471 1135 993 773 681 219 191 142
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5605
SmaI CCC'GGG
Cuts at: 1872 2028 2932 1872
Size: 156 904 4545
Fragments arranged by size:
4545 904 156
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3709 4610 4872 820
Size: 197 926 1766 901 262 1553
Fragments arranged by size:
1766 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3396 493
Size: 2903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SspI AAT'ATT
Cuts at: 5 52 3529 5
Size: 47 3477 2081
Fragments arranged by size:
3477 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5605
StyI C'CwwG_G
Cuts at: 513 1065 2004 3044 513
Size: 552 939 1040 3074
Fragments arranged by size:
3074 1040 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2333 2365 2612 2735 3632 3649 3802
Size: 1255 753 32 247 123 897 17 153
Cuts at: 3802 3987 4432 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 897 753 445 247 185 153
123 32 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3851 5563 96
Size: 274 80 33 51 1443 1874 1712 138
Fragments arranged by size:
1874 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5565 5597 5565
Size: 32 5573
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5605
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5605
XmaI C'CCGG_G
Cuts at: 1870 2026 2930 1870
Size: 156 904 4545
Fragments arranged by size:
4545 904 156
XmnI GAAnn'nnTTC
Cuts at: 3734 3734
Size: 5605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3413 276
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI