Restriction map for
pFN21AB6382
(Circular) (Six-base) MAPSORT of: pFN21AB6382.seq Check: 4596 from: 1 to: 5605
pFN21AB6382 5605 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3415 278
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2948 1937
Size: 1011 4594
Acc65I G'GTAC_C
Cuts at: 2926 2926
Size: 5605
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 2482 3668 5052 5320 1632
Size: 557 293 1186 1384 268 1917
Fragments arranged by size:
1917 1384 1186 557 293 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 2739 2795 4504 5481 1203
Size: 1536 56 1709 977 1327
Fragments arranged by size:
1709 1536 1327 977 56
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 4334 4334
Size: 5605
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5605
AlwNI CAG_nnn'CTG
Cuts at: 3348 4920 3348
Size: 1572 4033
ApaLI G'TGCA_C
Cuts at: 3662 4818 3662
Size: 1156 4449
ApoI r'AATT_y
Cuts at: 1493 2189 2914 3140 3292 5401 1493
Size: 696 725 226 152 2109 1697
Fragments arranged by size:
2109 1697 725 696 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2712 2930 1870
Size: 73 769 218 4545
Fragments arranged by size:
4545 769 218 73
BamHI G'GATC_C
Cuts at: 2935 3302 2935
Size: 367 5238
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2926 4381 618
Size: 325 217 627 1139 1455 1842
Fragments arranged by size:
1842 1455 1139 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2924 1391
Size: 1533 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2488 2894 928
Size: 971 589 406 3639
Fragments arranged by size:
3639 971 589 406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3796 3830 1903
Size: 34 1859 34 3678
Fragments arranged by size:
3678 1859 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2267 3497 4707 2267
Size: 1230 1210 3165
Fragments arranged by size:
3165 1230 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5605
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4216 136
Size: 107 122 71 631 605 2544 1525
Fragments arranged by size:
2544 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5600 5600
Size: 5605
BlpI GC'TnA_GC
Cuts at: 3022 3022
Size: 5605
Bme1580I G_kGCm'C
Cuts at: 1790 3666 4822 1790
Size: 1876 1156 2573
Fragments arranged by size:
2573 1876 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3336 4294 442
Size: 416 53 83 399 24 1919 958 1753
Fragments arranged by size:
1919 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2645 4265 1239
Size: 797 609 1620 2579
Fragments arranged by size:
2579 1620 797 609
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2039 2810 3730 4595 4893 2039
Size: 771 920 865 298 2751
Fragments arranged by size:
2751 920 865 771 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2447 4268 882
Size: 617 948 1821 2219
Fragments arranged by size:
2219 1821 948 617
BsaAI yAC'GTr
Cuts at: 493 1204 2798 3396 493
Size: 711 1594 598 2702
Fragments arranged by size:
2702 1594 711 598
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2643 3412 3794 4429
Size: 53 83 186 936 1110 769 382 635
Cuts at: 4429 275
Size: 1451
Fragments arranged by size:
1451 1110 936 769 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2086 4038 4436 4710 4857 5268 2086
Size: 1952 398 274 147 411 2423
Fragments arranged by size:
2423 1952 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5380
Fragments arranged by size:
5380 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2610 1186
Size: 868 556 4181
Fragments arranged by size:
4181 868 556
BseYI C'CCAG_C
Cuts at: 4808 5304 4808
Size: 496 5109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2741 1163
Size: 573 1005 4027
Fragments arranged by size:
4027 1005 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2073 3055 3816 4844 1540
Size: 275 186 72 982 761 1028 2301
Fragments arranged by size:
2301 1028 982 761 275 186 72
BsiHKAI G_wGCw'C
Cuts at: 2713 2777 3666 3751 4822 2713
Size: 64 889 85 1071 3496
Fragments arranged by size:
3496 1071 889 85 64
BsmI GAATG_Cn'
Cuts at: 3116 3209 3116
Size: 93 5512
BsmBI CGTCTCn'nnnn_
Cuts at: 2036 2036
Size: 5605
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2713 2777 2924 3666 3751 4822 1391
Size: 399 923 64 147 742 85 1071 2174
Fragments arranged by size:
2174 1071 923 742 399 147 85 64
BspEI T'CCGG_A
Cuts at: 5268 5268
Size: 5605
BspHI T'CATG_A
Cuts at: 3494 5224 3494
Size: 1730 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2763 2960 844
Size: 1919 197 3489
Fragments arranged by size:
3489 1919 197
BsrBI CCG'CTC
Cuts at: 1394 1823 3492 1394
Size: 429 1669 3507
Fragments arranged by size:
3507 1669 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4100 4274 65
Size: 1183 2852 174 1396
Fragments arranged by size:
2852 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4249 4436 1700
Size: 110 2439 187 2869
Fragments arranged by size:
2869 2439 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5605
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5605
BssSI C'ACGA_G
Cuts at: 3665 4677 3665
Size: 1012 4593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2216 794
Size: 1422 4183
BstBI TT'CG_AA
Cuts at: 4409 4409
Size: 5605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5605
BstYI r'GATC_y
Cuts at: 1972 2935 3302 3687 3704 5156 5242 5254 5600
Size: 963 367 385 17 1452 86 12 346
Cuts at: 5600 1972
Size: 1977
Fragments arranged by size:
1977 1452 963 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5053
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4866
Fragments arranged by size:
4866 683 56
BtsI GCAGTG_nn'
Cuts at: 3121 3915 3935 3121
Size: 794 20 4791
Fragments arranged by size:
4791 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5570
DraI TTT'AAA
Cuts at: 2909 2909
Size: 5605
DraIII CAC_nnn'GTG
Cuts at: 2216 2216
Size: 5605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2528 4612 809
Size: 507 1212 2084 1802
Fragments arranged by size:
2084 1802 1212 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 3052 3941
Size: 54 1425 50 275 18 240 982 889
Cuts at: 3941 5570 8
Size: 1629 43
Fragments arranged by size:
1629 1425 982 889 275 240 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2070 3052 1537
Size: 275 258 982 4090
Fragments arranged by size:
4090 982 275 258
EarI CTCTTCn'nnn_
Cuts at: 1353 2397 3535 1353
Size: 1044 1138 3423
Fragments arranged by size:
3423 1138 1044
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4192 4566 4712 387
Size: 1064 138 2603 374 146 1280
Fragments arranged by size:
2603 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2189 2482 2645 3668 4265 5052
Size: 393 404 153 293 163 1023 597 787
Cuts at: 5052 5320 1239
Size: 268 1524
Fragments arranged by size:
1524 1023 787 597 404 393 293 268
163 153
EcoNI CCTnn'n_nnAGG
Cuts at: 2015 2015
Size: 5605
EcoO109I rG'GnC_Cy
Cuts at: 2125 2596 2125
Size: 471 5134
EcoRI G'AATT_C
Cuts at: 2914 3292 2914
Size: 378 5227
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 4752 1678
Size: 3074 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2265 2297 4246 4278 4320
Size: 32 135 32 693 32 1949 32 42
Cuts at: 4320 4352 1373
Size: 32 2626
Fragments arranged by size:
2626 1949 693 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2949 3195 669
Size: 988 281 1011 246 3079
Fragments arranged by size:
3079 1011 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5605
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KpnI G_GTAC'C
Cuts at: 2930 2930
Size: 5605
MfeI C'AATT_G
Cuts at: 3204 3204
Size: 5605
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4719 4903 1305
Size: 3414 184 2007
Fragments arranged by size:
3414 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3783
Fragments arranged by size:
3783 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2700 2798 3563 3922 4081 518
Size: 648 1534 98 765 359 159 2042
Fragments arranged by size:
2042 1534 765 648 359 159 98
MspA1I CmG'CkG
Cuts at: 1606 2648 3021 3698 4846 5091 1606
Size: 1042 373 677 1148 245 2120
Fragments arranged by size:
2120 1148 1042 677 373 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5053
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5605
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 2070 3052 2070
Size: 982 4623
NspI r_CATG'y
Cuts at: 1132 1229 2350 2963 4508 1132
Size: 97 1121 613 1545 2229
Fragments arranged by size:
2229 1545 1121 613 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5605
PciI A'CATG_T
Cuts at: 4504 4504
Size: 5605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5605
PfoI T'CCnGG_A
Cuts at: 2745 2745
Size: 5605
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 5187 5219 3674
Size: 32 1481 32 4060
Fragments arranged by size:
4060 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2596 2125
Size: 471 5134
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 3175 3175
Size: 5605
PstI C_TGCA'G
Cuts at: 830 2957 830
Size: 2127 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
SalI G'TCGA_C
Cuts at: 1936 2947 1936
Size: 1011 4594
SbfI CC_TGCA'GG
Cuts at: 2957 2957
Size: 5605
ScaI AGT'ACT
Cuts at: 3853 5565 3853
Size: 1712 3893
SfcI C'TryA_G
Cuts at: 826 1045 2953 4088 4769 4960 826
Size: 219 1908 1135 681 191 1471
Fragments arranged by size:
1908 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5605
SmaI CCC'GGG
Cuts at: 1872 2932 1872
Size: 1060 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2054 2712 2825 3709 4610 4872
Size: 197 926 111 658 113 884 901 262
Cuts at: 4872 820
Size: 1553
Fragments arranged by size:
1553 926 901 884 658 262 197 113
111
SnaBI TAC'GTA
Cuts at: 493 3396 493
Size: 2903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SspI AAT'ATT
Cuts at: 5 52 3529 5
Size: 47 3477 2081
Fragments arranged by size:
3477 2081 47
StuI AGG'CCT
Cuts at: 1517 2783 1517
Size: 1266 4339
StyI C'CwwG_G
Cuts at: 513 1065 2319 3044 513
Size: 552 1254 725 3074
Fragments arranged by size:
3074 1254 725 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2642 3632 3649 3802 3987 4432 325
Size: 1255 1062 990 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1062 990 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3851 5563 96
Size: 274 80 33 51 1443 1874 1712 138
Fragments arranged by size:
1874 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5565 5597 5565
Size: 32 5573
Tth111I GACn'n_nGTC
Cuts at: 1316 2483 1316
Size: 1167 4438
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5605
XhoI C'TCGA_G
Cuts at: 1943 2712 1943
Size: 769 4836
XmaI C'CCGG_G
Cuts at: 1870 2930 1870
Size: 1060 4545
XmnI GAAnn'nnTTC
Cuts at: 2307 3734 2307
Size: 1427 4178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3413 276
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI