Restriction map for
pFN21AB6380
(Circular) (Six-base) MAPSORT of: pFN21AB6380.seq Check: 6287 from: 1 to: 5602
pFN21AB6380 5602 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3412 278
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2945 1937
Size: 1008 4594
Acc65I G'GTAC_C
Cuts at: 2923 2923
Size: 5602
AclI AA'CG_TT
Cuts at: 1563 3729 4102 1563
Size: 2166 373 3063
Fragments arranged by size:
3063 2166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2442 2607 3665 5049 5317 1632
Size: 810 165 1058 1384 268 1917
Fragments arranged by size:
1917 1384 1058 810 268 165
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5405
AflIII A'CryG_T
Cuts at: 1203 4501 5478 1203
Size: 3298 977 1327
Fragments arranged by size:
3298 1327 977
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 4331 4331
Size: 5602
AleI CACnn'nnGTG
Cuts at: 1166 2504 1166
Size: 1338 4264
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5602
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2775 2807 2775
Size: 32 5570
AlwNI CAG_nnn'CTG
Cuts at: 2249 3345 4917 2249
Size: 1096 1572 2934
Fragments arranged by size:
2934 1572 1096
ApaLI G'TGCA_C
Cuts at: 3659 4815 3659
Size: 1156 4446
ApoI r'AATT_y
Cuts at: 1493 2012 2911 3137 3289 5398 1493
Size: 519 899 226 152 2109 1697
Fragments arranged by size:
2109 1697 899 519 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4156 160
Size: 872 3124 1606
Fragments arranged by size:
3124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2927 1870
Size: 73 984 4545
Fragments arranged by size:
4545 984 73
BamHI G'GATC_C
Cuts at: 2932 3299 2932
Size: 367 5235
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2699 2923 4378 618
Size: 325 217 627 912 224 1455 1842
Fragments arranged by size:
1842 1455 912 627 325 224 217
BanII G_rGCy'C
Cuts at: 1391 2921 1391
Size: 1530 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2031 2200 928
Size: 971 132 169 4330
Fragments arranged by size:
4330 971 169 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3793 3827 1903
Size: 34 1856 34 3678
Fragments arranged by size:
3678 1856 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3494 4704 3494
Size: 1210 4392
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4213 136
Size: 107 122 71 631 605 2541 1525
Fragments arranged by size:
2541 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2875 5597 2875
Size: 2722 2880
BlpI GC'TnA_GC
Cuts at: 2885 3019 2885
Size: 134 5468
Bme1580I G_kGCm'C
Cuts at: 1790 2388 3663 4819 1790
Size: 598 1275 1156 2573
Fragments arranged by size:
2573 1275 1156 598
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5602
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2755 3333 4291
Size: 416 53 83 399 24 1338 578 958
Cuts at: 4291 442
Size: 1753
Fragments arranged by size:
1753 1338 958 578 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4262 1239
Size: 3023 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2747 3727 4592 4890 2747
Size: 980 865 298 3459
Fragments arranged by size:
3459 980 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4265 882
Size: 617 2766 2219
Fragments arranged by size:
2766 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2059 3393 493
Size: 711 855 1334 2702
Fragments arranged by size:
2702 1334 855 711
BsaBI GATnn'nnATC
Cuts at: 5596 5596
Size: 5602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3409 3791 4426 275
Size: 53 83 186 936 1876 382 635 1451
Fragments arranged by size:
1876 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4035 4433 4707 4854 5265 4035
Size: 398 274 147 411 4372
Fragments arranged by size:
4372 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5377
Fragments arranged by size:
5377 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2243 2282 2429 1186
Size: 871 186 39 147 4359
Fragments arranged by size:
4359 871 186 147 39
BseYI C'CCAG_C
Cuts at: 4805 5301 4805
Size: 496 5106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5029
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2842 3052 3813 4841 1540
Size: 275 186 841 210 761 1028 2301
Fragments arranged by size:
2301 1028 841 761 275 210 186
BsiHKAI G_wGCw'C
Cuts at: 2723 3663 3748 4819 2723
Size: 940 85 1071 3506
Fragments arranged by size:
3506 1071 940 85
BsmI GAATG_Cn'
Cuts at: 2175 3113 3206 2175
Size: 938 93 4571
Fragments arranged by size:
4571 938 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2388 2723 2921 3663 3748 4819 1391
Size: 399 598 335 198 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 598 399 335 198 85
BspEI T'CCGG_A
Cuts at: 5265 5265
Size: 5602
BspHI T'CATG_A
Cuts at: 3491 5221 3491
Size: 1730 3872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2957 844
Size: 2113 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3489 1394
Size: 429 1666 3507
Fragments arranged by size:
3507 1666 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4097 4271 65
Size: 1183 2849 174 1396
Fragments arranged by size:
2849 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4246 4433 1700
Size: 110 2436 187 2869
Fragments arranged by size:
2869 2436 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5602
BssSI C'ACGA_G
Cuts at: 3662 4674 3662
Size: 1012 4590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5602
BstBI TT'CG_AA
Cuts at: 4406 4406
Size: 5602
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5602
BstYI r'GATC_y
Cuts at: 1972 2875 2932 3299 3684 3701 5153 5239 5251
Size: 903 57 367 385 17 1452 86 12
Cuts at: 5251 5597 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 903 385 367 346 86 57
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4111
Fragments arranged by size:
4111 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4863
Fragments arranged by size:
4863 683 56
BtsI GCAGTG_nn'
Cuts at: 3118 3912 3932 3118
Size: 794 20 4788
Fragments arranged by size:
4788 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5567
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4609 809
Size: 507 3293 1802
Fragments arranged by size:
3293 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2787 3049 3938
Size: 54 1425 50 275 18 957 262 889
Cuts at: 3938 5567 8
Size: 1629 43
Fragments arranged by size:
1629 1425 957 889 275 262 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3049 1537
Size: 275 1237 4090
Fragments arranged by size:
4090 1237 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2124 2183 2214 2220 2418 2454 2760 3532
Size: 771 59 31 6 198 36 306 772
Cuts at: 3532 1353
Size: 3423
Fragments arranged by size:
3423 772 771 306 198 59 36 31
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4189 4563 4709 387
Size: 1064 138 2600 374 146 1280
Fragments arranged by size:
2600 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2442 2607 3665 4262 5049 5317 1239
Size: 393 810 165 1058 597 787 268 1524
Fragments arranged by size:
1524 1058 810 787 597 393 268 165
EcoRI G'AATT_C
Cuts at: 2012 2911 3289 2012
Size: 899 378 4325
Fragments arranged by size:
4325 899 378
FspI TGC'GCA
Cuts at: 4108 4108
Size: 5602
HaeII r_GCGC'y
Cuts at: 1678 4749 1678
Size: 3071 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2543 2558 2575 2590 4243
Size: 32 135 32 971 15 17 15 1653
Cuts at: 4243 4275 4317 4349 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1653 971 135 42 32 32 32
32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2946 3192 669
Size: 988 281 1008 246 3079
Fragments arranged by size:
3079 1008 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5602
HpaI GTT'AAC
Cuts at: 3192 3192
Size: 5602
KpnI G_GTAC'C
Cuts at: 2927 2927
Size: 5602
MfeI C'AATT_G
Cuts at: 3201 3201
Size: 5602
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2019 4716 4900 1305
Size: 714 2697 184 2007
Fragments arranged by size:
2697 2007 714 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2789 10
Size: 54 1425 343 957 2823
Fragments arranged by size:
2823 1425 957 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2504 3560 3919 4078 518
Size: 648 1338 1056 359 159 2042
Fragments arranged by size:
2042 1338 1056 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3018 3695 4843 5088 1606
Size: 1412 677 1148 245 2120
Fragments arranged by size:
2120 1412 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4111
Fragments arranged by size:
4111 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5602
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5602
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2960 4505 1132
Size: 97 1731 1545 2229
Fragments arranged by size:
2229 1731 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5602
PciI A'CATG_T
Cuts at: 4501 4501
Size: 5602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5602
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 5184 5216 3671
Size: 32 1481 32 4057
Fragments arranged by size:
4057 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5602
PsiI TTA'TAA
Cuts at: 3172 3172
Size: 5602
PstI C_TGCA'G
Cuts at: 830 2287 2954 830
Size: 1457 667 3478
Fragments arranged by size:
3478 1457 667
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5602
SalI G'TCGA_C
Cuts at: 1936 2944 1936
Size: 1008 4594
SapI GCTCTTCn'nnn_
Cuts at: 2124 2454 2124
Size: 330 5272
SbfI CC_TGCA'GG
Cuts at: 2954 2954
Size: 5602
ScaI AGT'ACT
Cuts at: 3850 5562 3850
Size: 1712 3890
SfcI C'TryA_G
Cuts at: 826 1045 2283 2950 4085 4766 4957 826
Size: 219 1238 667 1135 681 191 1471
Fragments arranged by size:
1471 1238 1135 681 667 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2929 1872
Size: 1057 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2726 3706 4607 4869 820
Size: 197 926 783 980 901 262 1553
Fragments arranged by size:
1553 980 926 901 783 262 197
SnaBI TAC'GTA
Cuts at: 493 3393 493
Size: 2900 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5602
SphI G_CATG'C
Cuts at: 1132 2960 1132
Size: 1828 3774
SspI AAT'ATT
Cuts at: 5 52 3526 5
Size: 47 3474 2081
Fragments arranged by size:
3474 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5602
StyI C'CwwG_G
Cuts at: 513 1065 2004 2149 2502 3041 513
Size: 552 939 145 353 539 3074
Fragments arranged by size:
3074 939 552 539 353 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2856 3629 3646 3799 3984 4429 325
Size: 1255 1276 773 17 153 185 445 1498
Fragments arranged by size:
1498 1276 1255 773 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2393 3848 5560
Size: 274 80 33 51 1443 416 1455 1712
Cuts at: 5560 96
Size: 138
Fragments arranged by size:
1712 1455 1443 416 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2683 2715 5562 5594 2683
Size: 32 2847 32 2691
Fragments arranged by size:
2847 2691 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5602
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5602
XmaI C'CCGG_G
Cuts at: 1870 2927 1870
Size: 1057 4545
XmnI GAAnn'nnTTC
Cuts at: 2831 3731 2831
Size: 900 4702
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3410 276
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI