Restriction map for
pFN21AB6378
(Circular) (Six-base) MAPSORT of: pFN21AB6378.seq Check: 9481 from: 1 to: 5602
pFN21AB6378 5602 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3412 278
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2850 2945 1937
Size: 913 95 4594
Fragments arranged by size:
4594 913 95
Acc65I G'GTAC_C
Cuts at: 2923 2923
Size: 5602
AclI AA'CG_TT
Cuts at: 1563 3729 4102 1563
Size: 2166 373 3063
Fragments arranged by size:
3063 2166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3665 5049 5317 1632
Size: 2033 1384 268 1917
Fragments arranged by size:
2033 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5405
AflIII A'CryG_T
Cuts at: 1203 4501 5478 1203
Size: 3298 977 1327
Fragments arranged by size:
3298 1327 977
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 4331 4331
Size: 5602
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5602
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2636 1184
Size: 1452 4150
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2754 2786 2806 2838 2754
Size: 32 20 32 5518
Fragments arranged by size:
5518 32 32 20
AlwNI CAG_nnn'CTG
Cuts at: 3345 4917 3345
Size: 1572 4030
ApaI G_GGCC'C
Cuts at: 2751 2751
Size: 5602
ApaLI G'TGCA_C
Cuts at: 3659 4815 3659
Size: 1156 4446
ApoI r'AATT_y
Cuts at: 1493 2911 3137 3289 5398 1493
Size: 1418 226 152 2109 1697
Fragments arranged by size:
2109 1697 1418 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4156 160
Size: 872 3124 1606
Fragments arranged by size:
3124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2927 1870
Size: 73 984 4545
Fragments arranged by size:
4545 984 73
BamHI G'GATC_C
Cuts at: 2932 3299 2932
Size: 367 5235
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2923 4378 618
Size: 325 217 627 1136 1455 1842
Fragments arranged by size:
1842 1455 1136 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2093 2174 2414 2751 2837 2921 1391
Size: 702 81 240 337 86 84 4072
Fragments arranged by size:
4072 702 337 240 86 84 81
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4631
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3793 3827 1903
Size: 34 50 34 1772 34 3678
Fragments arranged by size:
3678 1772 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3494 4704 3494
Size: 1210 4392
BclI T'GATC_A
Cuts at: 1548 2058 2392 1548
Size: 510 334 4758
Fragments arranged by size:
4758 510 334
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2457 2633 4213
Size: 107 122 71 631 605 785 176 1580
Cuts at: 4213 136
Size: 1525
Fragments arranged by size:
1580 1525 785 631 605 176 122 107
71
BglII A'GATC_T
Cuts at: 5597 5597
Size: 5602
BlpI GC'TnA_GC
Cuts at: 2831 3019 2831
Size: 188 5414
Bme1580I G_kGCm'C
Cuts at: 1790 2303 2751 3663 4819 1790
Size: 513 448 912 1156 2573
Fragments arranged by size:
2573 1156 912 513 448
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5602
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3333 4291 442
Size: 416 53 83 399 24 1916 958 1753
Fragments arranged by size:
1916 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5602
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 5602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2094 2442 4262 1239
Size: 855 348 1820 2579
Fragments arranged by size:
2579 1820 855 348
Bpu10I CC'TnA_GC
Cuts at: 2092 2092
Size: 5602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3727 4592 4890 3727
Size: 865 298 4439
Fragments arranged by size:
4439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2502 4265 882
Size: 617 1003 1763 2219
Fragments arranged by size:
2219 1763 1003 617
BsaAI yAC'GTr
Cuts at: 493 1204 3393 493
Size: 711 2189 2702
Fragments arranged by size:
2702 2189 711
BsaBI GATnn'nnATC
Cuts at: 5596 5596
Size: 5602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3409 3791 4426 275
Size: 53 83 186 936 1876 382 635 1451
Fragments arranged by size:
1876 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4035 4433 4707 4854 5265 4035
Size: 398 274 147 411 4372
Fragments arranged by size:
4372 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2135 2156 2165 2186 1178
Size: 30 165 30 732 21 9 21 4594
Fragments arranged by size:
4594 732 165 30 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2478 2481 2909 1186
Size: 1292 3 428 3879
Fragments arranged by size:
3879 1292 428 3
BseYI C'CCAG_C
Cuts at: 2296 2426 2592 4805 5301 2296
Size: 130 166 2213 496 2597
Fragments arranged by size:
2597 2213 496 166 130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5029
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3052 3813 4841 1540
Size: 275 186 1051 761 1028 2301
Fragments arranged by size:
2301 1051 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2093 2837 3663 3748 4819 2093
Size: 744 826 85 1071 2876
Fragments arranged by size:
2876 1071 826 744 85
BsmI GAATG_Cn'
Cuts at: 3113 3206 3113
Size: 93 5509
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2174 2303 2414 2751 2837 2921
Size: 399 303 81 129 111 337 86 84
Cuts at: 2921 3663 3748 4819 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 337 303 129 111
86 85 84 81
BspEI T'CCGG_A
Cuts at: 5265 5265
Size: 5602
BspHI T'CATG_A
Cuts at: 3491 5221 3491
Size: 1730 3872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2628 2957 844
Size: 1784 329 3489
Fragments arranged by size:
3489 1784 329
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 3489 1394
Size: 429 188 1478 3507
Fragments arranged by size:
3507 1478 429 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 2685 4097 4271 65
Size: 1183 1437 1412 174 1396
Fragments arranged by size:
1437 1412 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4246 4433 1700
Size: 110 2436 187 2869
Fragments arranged by size:
2869 2436 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5602
BssSI C'ACGA_G
Cuts at: 3662 4674 3662
Size: 1012 4590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5602
BstBI TT'CG_AA
Cuts at: 4406 4406
Size: 5602
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5602
BstYI r'GATC_y
Cuts at: 1972 2932 3299 3684 3701 5153 5239 5251 5597
Size: 960 367 385 17 1452 86 12 346
Cuts at: 5597 1972
Size: 1977
Fragments arranged by size:
1977 1452 960 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2445 2564 2577 513
Size: 552 1380 119 13 3538
Fragments arranged by size:
3538 1380 552 119 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4863
Fragments arranged by size:
4863 683 56
BtsI GCAGTG_nn'
Cuts at: 2371 3118 3912 3932 2371
Size: 747 794 20 4041
Fragments arranged by size:
4041 794 747 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5567
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4609 809
Size: 507 3293 1802
Fragments arranged by size:
3293 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3049 3938 5567
Size: 54 1425 50 275 18 1219 889 1629
Cuts at: 5567 8
Size: 43
Fragments arranged by size:
1629 1425 1219 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3049 1537
Size: 275 1237 4090
Fragments arranged by size:
4090 1237 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2033 3532 1353
Size: 680 1499 3423
Fragments arranged by size:
3423 1499 680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2535 4189 4563 4709 387
Size: 1064 138 946 1654 374 146 1280
Fragments arranged by size:
1654 1280 1064 946 374 146 138
EcoICRI GAG'CTC
Cuts at: 2091 2835 2091
Size: 744 4858
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2442 3665 4262 5049 5317 1239
Size: 393 462 348 1223 597 787 268 1524
Fragments arranged by size:
1524 1223 787 597 462 393 348 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2668 2668
Size: 5602
EcoO109I rG'GnC_Cy
Cuts at: 2747 2748 2747
Size: 1 5601
EcoRI G'AATT_C
Cuts at: 2911 3289 2911
Size: 378 5224
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2663 2663
Size: 5602
FspI TGC'GCA
Cuts at: 4108 4108
Size: 5602
HaeII r_GCGC'y
Cuts at: 1678 4749 1678
Size: 3071 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2225 2257 2486
Size: 32 135 32 563 32 58 32 229
Cuts at: 2486 2518 4243 4275 4317 4349 1373
Size: 32 1725 32 42 32 2626
Fragments arranged by size:
2626 1725 563 229 135 58 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2587 2946 3192 669
Size: 988 281 649 359 246 3079
Fragments arranged by size:
3079 988 649 359 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5602
HpaI GTT'AAC
Cuts at: 3192 3192
Size: 5602
KpnI G_GTAC'C
Cuts at: 2927 2927
Size: 5602
MfeI C'AATT_G
Cuts at: 3201 3201
Size: 5602
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2121 2303 2817 4716 4900 1305
Size: 816 182 514 1899 184 2007
Fragments arranged by size:
2007 1899 816 514 184 182
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3780
Fragments arranged by size:
3780 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2232 3560 3919 4078 518
Size: 648 1066 1328 359 159 2042
Fragments arranged by size:
2042 1328 1066 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2296 2306 2447 3018 3695 4843 5088 1606
Size: 690 10 141 571 677 1148 245 2120
Fragments arranged by size:
2120 1148 690 677 571 245 141 10
NcoI C'CATG_G
Cuts at: 513 1065 2564 2577 513
Size: 552 1499 13 3538
Fragments arranged by size:
3538 1499 552 13
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5602
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5602
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2960 4505 1132
Size: 97 1731 1545 2229
Fragments arranged by size:
2229 1731 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5602
PciI A'CATG_T
Cuts at: 4501 4501
Size: 5602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5602
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 5184 5216 3671
Size: 32 1481 32 4057
Fragments arranged by size:
4057 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5602
PsiI TTA'TAA
Cuts at: 3172 3172
Size: 5602
PspOMI G'GGCC_C
Cuts at: 2747 2747
Size: 5602
PstI C_TGCA'G
Cuts at: 830 2954 830
Size: 2124 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5602
PvuII CAG'CTG
Cuts at: 2296 2306 2296
Size: 10 5592
SacI G_AGCT'C
Cuts at: 2093 2837 2093
Size: 744 4858
SacII CC_GC'GG
Cuts at: 2448 2448
Size: 5602
SalI G'TCGA_C
Cuts at: 1936 2944 1936
Size: 1008 4594
SbfI CC_TGCA'GG
Cuts at: 2954 2954
Size: 5602
ScaI AGT'ACT
Cuts at: 3850 5562 3850
Size: 1712 3890
SfcI C'TryA_G
Cuts at: 826 1045 2847 2950 4085 4766 4957 826
Size: 219 1802 103 1135 681 191 1471
Fragments arranged by size:
1802 1471 1135 681 219 191 103
SfiI GGCCn_nnn'nGGCC
Cuts at: 2633 2633
Size: 5602
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2929 1872
Size: 1057 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3706 4607 4869 820
Size: 197 926 1763 901 262 1553
Fragments arranged by size:
1763 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3393 493
Size: 2900 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5602
SphI G_CATG'C
Cuts at: 1132 2960 1132
Size: 1828 3774
SspI AAT'ATT
Cuts at: 5 52 3526 5
Size: 47 3474 2081
Fragments arranged by size:
3474 2081 47
StuI AGG'CCT
Cuts at: 1517 2705 2800 1517
Size: 1188 95 4319
Fragments arranged by size:
4319 1188 95
StyI C'CwwG_G
Cuts at: 513 1065 2126 2564 2577 2877 3041 513
Size: 552 1061 438 13 300 164 3074
Fragments arranged by size:
3074 1061 552 438 300 164 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3629 3646 3799 3984 4429 325
Size: 1255 2049 17 153 185 445 1498
Fragments arranged by size:
2049 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3848 5560 96
Size: 274 80 33 51 1443 1871 1712 138
Fragments arranged by size:
1871 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5562 5594 5562
Size: 32 5570
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5602
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2411 2740 2411
Size: 329 5273
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5602
XmaI C'CCGG_G
Cuts at: 1870 2927 1870
Size: 1057 4545
XmnI GAAnn'nnTTC
Cuts at: 3731 3731
Size: 5602
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3410 276
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SapI
SexAI SfoI SrfI SwaI