Restriction map for
pFN21AB6377
(Circular) (Six-base) MAPSORT of: pFN21AB6377.seq Check: 4171 from: 1 to: 5518
pFN21AB6377 5518 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3328 278
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2861 1937
Size: 924 4594
Acc65I G'GTAC_C
Cuts at: 2839 2839
Size: 5518
AclI AA'CG_TT
Cuts at: 1563 3645 4018 1563
Size: 2082 373 3063
Fragments arranged by size:
3063 2082 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2057 2267 2636 3581 4965 5233 1632
Size: 425 210 369 945 1384 268 1917
Fragments arranged by size:
1917 1384 945 425 369 268 210
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5321
AflIII A'CryG_T
Cuts at: 1203 2022 4417 5394 1203
Size: 819 2395 977 1327
Fragments arranged by size:
2395 1327 977 819
AgeI A'CCGG_T
Cuts at: 4349 4349
Size: 5518
AhdI GACnn_n'nnGTC
Cuts at: 4247 4247
Size: 5518
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5518
AlwNI CAG_nnn'CTG
Cuts at: 2803 3261 4833 2803
Size: 458 1572 3488
Fragments arranged by size:
3488 1572 458
ApaLI G'TGCA_C
Cuts at: 3575 4731 3575
Size: 1156 4362
ApoI r'AATT_y
Cuts at: 1493 2402 2498 2827 3053 3205 5314 1493
Size: 909 96 329 226 152 2109 1697
Fragments arranged by size:
2109 1697 909 329 226 152 96
AseI AT'TA_AT
Cuts at: 160 1032 4072 160
Size: 872 3040 1606
Fragments arranged by size:
3040 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
AvaI C'yCGr_G
Cuts at: 1870 1943 2843 1870
Size: 73 900 4545
Fragments arranged by size:
4545 900 73
AvrII C'CTAG_G
Cuts at: 2336 2336
Size: 5518
BamHI G'GATC_C
Cuts at: 2848 3215 2848
Size: 367 5151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2839 4294 618
Size: 325 217 627 1052 1455 1842
Fragments arranged by size:
1842 1455 1052 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2837 1391
Size: 1446 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2037 2070 2604 928
Size: 971 138 33 534 3842
Fragments arranged by size:
3842 971 534 138 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3709 3743 1903
Size: 34 1772 34 3678
Fragments arranged by size:
3678 1772 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3410 4620 3410
Size: 1210 4308
BclI T'GATC_A
Cuts at: 1548 2080 2449 1548
Size: 532 369 4617
Fragments arranged by size:
4617 532 369
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4129 136
Size: 107 122 71 631 605 2457 1525
Fragments arranged by size:
2457 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5513 5513
Size: 5518
BlpI GC'TnA_GC
Cuts at: 2935 2935
Size: 5518
Bme1580I G_kGCm'C
Cuts at: 1790 3579 4735 1790
Size: 1789 1156 2573
Fragments arranged by size:
2573 1789 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5518
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2764 3249 4207
Size: 416 53 83 399 24 1347 485 958
Cuts at: 4207 442
Size: 1753
Fragments arranged by size:
1753 1347 958 485 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 4178 1239
Size: 1076 1863 2579
Fragments arranged by size:
2579 1863 1076
Bpu10I CC'TnA_GC
Cuts at: 2360 2360
Size: 5518
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3643 4508 4806 3643
Size: 865 298 4355
Fragments arranged by size:
4355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2651 4181 882
Size: 617 1152 1530 2219
Fragments arranged by size:
2219 1530 1152 617
BsaAI yAC'GTr
Cuts at: 493 1204 3309 493
Size: 711 2105 2702
Fragments arranged by size:
2702 2105 711
BsaBI GATnn'nnATC
Cuts at: 5512 5512
Size: 5518
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2418 3325 3707 4342
Size: 53 83 186 936 885 907 382 635
Cuts at: 4342 275
Size: 1451
Fragments arranged by size:
1451 936 907 885 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3951 4349 4623 4770 5181 3951
Size: 398 274 147 411 4288
Fragments arranged by size:
4288 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2057 2087 1178
Size: 30 165 30 654 30 4609
Fragments arranged by size:
4609 654 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2657 1186
Size: 1471 4047
BseYI C'CCAG_C
Cuts at: 4721 5217 4721
Size: 496 5022
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4945
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2968 3729 4757 1540
Size: 275 186 967 761 1028 2301
Fragments arranged by size:
2301 1028 967 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3579 3664 4735 3579
Size: 85 1071 4362
Fragments arranged by size:
4362 1071 85
BsmI GAATG_Cn'
Cuts at: 3029 3122 3029
Size: 93 5425
BsmBI CGTCTCn'nnnn_
Cuts at: 2248 2248
Size: 5518
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2837 3579 3664 4735 1391
Size: 399 1047 742 85 1071 2174
Fragments arranged by size:
2174 1071 1047 742 399 85
BspEI T'CCGG_A
Cuts at: 5181 5181
Size: 5518
BspHI T'CATG_A
Cuts at: 3407 5137 3407
Size: 1730 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2873 844
Size: 2029 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3405 1394
Size: 429 1582 3507
Fragments arranged by size:
3507 1582 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2371 4013 4187 65
Size: 1183 1123 1642 174 1396
Fragments arranged by size:
1642 1396 1183 1123 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4162 4349 1700
Size: 110 2352 187 2869
Fragments arranged by size:
2869 2352 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5518
BssSI C'ACGA_G
Cuts at: 3578 4590 3578
Size: 1012 4506
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5518
BstBI TT'CG_AA
Cuts at: 4322 4322
Size: 5518
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5518
BstYI r'GATC_y
Cuts at: 1972 2848 3215 3600 3617 5069 5155 5167 5513
Size: 876 367 385 17 1452 86 12 346
Cuts at: 5513 1972
Size: 1977
Fragments arranged by size:
1977 1452 876 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4027
Fragments arranged by size:
4027 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2287 487
Size: 683 56 1061 3718
Fragments arranged by size:
3718 1061 683 56
BtsI GCAGTG_nn'
Cuts at: 2499 3034 3828 3848 2499
Size: 535 794 20 4169
Fragments arranged by size:
4169 794 535 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5518
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5483
DraI TTT'AAA
Cuts at: 2822 2822
Size: 5518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4525 809
Size: 507 3209 1802
Fragments arranged by size:
3209 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2965 3854
Size: 54 1425 50 275 18 177 958 889
Cuts at: 3854 5483 8
Size: 1629 43
Fragments arranged by size:
1629 1425 958 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2965 1537
Size: 275 1153 4090
Fragments arranged by size:
4090 1153 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2246 2675 3448 1353
Size: 893 429 773 3423
Fragments arranged by size:
3423 893 773 429
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4105 4479 4625 387
Size: 1064 138 2516 374 146 1280
Fragments arranged by size:
2516 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2267 2315 2636 3581 4178 4965
Size: 393 425 210 48 321 945 597 787
Cuts at: 4965 5233 1239
Size: 268 1524
Fragments arranged by size:
1524 945 787 597 425 393 321 268
210 48
EcoRI G'AATT_C
Cuts at: 2402 2827 3205 2402
Size: 425 378 4715
Fragments arranged by size:
4715 425 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 5518
FspI TGC'GCA
Cuts at: 4024 4024
Size: 5518
HaeII r_GCGC'y
Cuts at: 1678 4665 1678
Size: 2987 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2057 2072 2089 2104 4159
Size: 32 135 32 485 15 17 15 2055
Cuts at: 4159 4191 4233 4265 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2055 485 135 42 32 32 32
32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2862 3108 669
Size: 988 281 924 246 3079
Fragments arranged by size:
3079 988 924 281 246
HindIII A'AGCT_T
Cuts at: 748 2195 748
Size: 1447 4071
HpaI GTT'AAC
Cuts at: 3108 3108
Size: 5518
KpnI G_GTAC'C
Cuts at: 2843 2843
Size: 5518
MfeI C'AATT_G
Cuts at: 2573 3117 2573
Size: 544 4974
MluI A'CGCG_T
Cuts at: 5394 5394
Size: 5518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4632 4816 1305
Size: 3327 184 2007
Fragments arranged by size:
3327 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3519
Fragments arranged by size:
3519 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3476 3835 3994 518
Size: 648 2310 359 159 2042
Fragments arranged by size:
2310 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2934 3611 4759 5004 1606
Size: 1328 677 1148 245 2120
Fragments arranged by size:
2120 1328 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4027
Fragments arranged by size:
4027 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5518
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5518
NotI GC'GGCC_GC
Cuts at: 2965 2965
Size: 5518
NspI r_CATG'y
Cuts at: 1132 1229 2026 2876 4421 1132
Size: 97 797 850 1545 2229
Fragments arranged by size:
2229 1545 850 797 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5518
PciI A'CATG_T
Cuts at: 2022 4417 2022
Size: 2395 3123
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5518
PmeI GTTT'AAAC
Cuts at: 2822 2822
Size: 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 5100 5132 3587
Size: 32 1481 32 3973
Fragments arranged by size:
3973 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5518
PsiI TTA'TAA
Cuts at: 2517 3088 2517
Size: 571 4947
PstI C_TGCA'G
Cuts at: 830 2870 830
Size: 2040 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5518
SalI G'TCGA_C
Cuts at: 1936 2860 1936
Size: 924 4594
SbfI CC_TGCA'GG
Cuts at: 2870 2870
Size: 5518
ScaI AGT'ACT
Cuts at: 3766 5478 3766
Size: 1712 3806
SfcI C'TryA_G
Cuts at: 826 1045 2866 4001 4682 4873 826
Size: 219 1821 1135 681 191 1471
Fragments arranged by size:
1821 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5518
SmaI CCC'GGG
Cuts at: 1872 2845 1872
Size: 973 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3622 4523 4785 820
Size: 197 926 1679 901 262 1553
Fragments arranged by size:
1679 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3309 493
Size: 2816 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5518
SphI G_CATG'C
Cuts at: 1132 2876 1132
Size: 1744 3774
SspI AAT'ATT
Cuts at: 5 52 3442 5
Size: 47 3390 2081
Fragments arranged by size:
3390 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5518
StyI C'CwwG_G
Cuts at: 513 1065 2004 2336 2957 513
Size: 552 939 332 621 3074
Fragments arranged by size:
3074 939 621 552 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2498 2746 3545 3562 3715 3900 4345
Size: 1255 918 248 799 17 153 185 445
Cuts at: 4345 325
Size: 1498
Fragments arranged by size:
1498 1255 918 799 445 248 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3764 5476 96
Size: 274 80 33 51 1443 1787 1712 138
Fragments arranged by size:
1787 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5478 5510 5478
Size: 32 5486
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5518
XbaI T'CTAG_A
Cuts at: 2854 2854
Size: 5518
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5518
XmaI C'CCGG_G
Cuts at: 1870 2843 1870
Size: 973 4545
XmnI GAAnn'nnTTC
Cuts at: 2105 2702 3647 2105
Size: 597 945 3976
Fragments arranged by size:
3976 945 597
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3326 276
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI