Restriction map for
pFN21AB6375
(Circular) (Six-base) MAPSORT of: pFN21AB6375.seq Check: 9908 from: 1 to: 5515
pFN21AB6375 5515 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2202 2202
Size: 5515
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2858 1937
Size: 921 4594
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3578 4962 5230 1632
Size: 1946 1384 268 1917
Fragments arranged by size:
1946 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 2407 4414 5391 1203
Size: 1204 2007 977 1327
Fragments arranged by size:
2007 1327 1204 977
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5515
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5515
AlwNI CAG_nnn'CTG
Cuts at: 3258 4830 3258
Size: 1572 3943
ApaI G_GGCC'C
Cuts at: 2528 2528
Size: 5515
ApaLI G'TGCA_C
Cuts at: 3572 4728 3572
Size: 1156 4359
ApoI r'AATT_y
Cuts at: 1493 2489 2824 3050 3202 5311 1493
Size: 996 335 226 152 2109 1697
Fragments arranged by size:
2109 1697 996 335 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2840 1870
Size: 73 897 4545
Fragments arranged by size:
4545 897 73
BamHI G'GATC_C
Cuts at: 2845 3212 2845
Size: 367 5148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2836 4291 618
Size: 325 217 627 1049 1455 1842
Fragments arranged by size:
1842 1455 1049 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2145 2245 2528 2834 1391
Size: 754 100 283 306 4072
Fragments arranged by size:
4072 754 306 283 100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2325 2484 928
Size: 971 426 159 3959
Fragments arranged by size:
3959 971 426 159
BbvCI CC'TCA_GC
Cuts at: 2085 2085
Size: 5515
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3706 3740 1903
Size: 34 1769 34 3678
Fragments arranged by size:
3678 1769 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3407 4617 3407
Size: 1210 4305
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4126 136
Size: 107 122 71 631 605 2454 1525
Fragments arranged by size:
2454 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2184 2356 5510 2184
Size: 172 3154 2189
Fragments arranged by size:
3154 2189 172
BlpI GC'TnA_GC
Cuts at: 2453 2932 2453
Size: 479 5036
Bme1580I G_kGCm'C
Cuts at: 1790 2528 3576 4732 1790
Size: 738 1048 1156 2573
Fragments arranged by size:
2573 1156 1048 738
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5515
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2783 3246 4204
Size: 416 53 83 399 24 1366 463 958
Cuts at: 4204 442
Size: 1753
Fragments arranged by size:
1753 1366 958 463 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 4175 1239
Size: 1169 1767 2579
Fragments arranged by size:
2579 1767 1169
Bpu10I CC'TnA_GC
Cuts at: 2085 2085
Size: 5515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2000 3640 4505 4803 2000
Size: 1640 865 298 2712
Fragments arranged by size:
2712 1640 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4178 882
Size: 617 2679 2219
Fragments arranged by size:
2679 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2410 3306 493
Size: 711 1206 896 2702
Fragments arranged by size:
2702 1206 896 711
BsaBI GATnn'nnATC
Cuts at: 2055 5509 2055
Size: 3454 2061
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2659 3322 3704 4339
Size: 53 83 186 936 1126 663 382 635
Cuts at: 4339 275
Size: 1451
Fragments arranged by size:
1451 1126 936 663 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3948 4346 4620 4767 5178 3948
Size: 398 274 147 411 4285
Fragments arranged by size:
4285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5290
Fragments arranged by size:
5290 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5515
BseYI C'CCAG_C
Cuts at: 2482 4718 5214 2482
Size: 2236 496 2783
Fragments arranged by size:
2783 2236 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4942
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2965 3726 4754 1540
Size: 275 186 964 761 1028 2301
Fragments arranged by size:
2301 1028 964 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2454 3576 3661 4732 2454
Size: 1122 85 1071 3237
Fragments arranged by size:
3237 1122 1071 85
BsmI GAATG_Cn'
Cuts at: 2229 2275 3026 3119 2229
Size: 46 751 93 4625
Fragments arranged by size:
4625 751 93 46
BsmBI CGTCTCn'nnnn_
Cuts at: 2744 2744
Size: 5515
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2245 2454 2528 2834 3576 3661
Size: 399 355 100 209 74 306 742 85
Cuts at: 3661 4732 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 355 306 209 100
85 74
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 3404 5134 3404
Size: 1730 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2202 2870 844
Size: 1358 668 3489
Fragments arranged by size:
3489 1358 668
BsrBI CCG'CTC
Cuts at: 1394 1823 3402 1394
Size: 429 1579 3507
Fragments arranged by size:
3507 1579 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2430 4010 4184 65
Size: 1183 1182 1580 174 1396
Fragments arranged by size:
1580 1396 1183 1182 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4159 4346 1700
Size: 110 2349 187 2869
Fragments arranged by size:
2869 2349 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5515
BssSI C'ACGA_G
Cuts at: 2255 3575 4587 2255
Size: 1320 1012 3183
Fragments arranged by size:
3183 1320 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5515
BstBI TT'CG_AA
Cuts at: 2221 2734 4319 2221
Size: 513 1585 3417
Fragments arranged by size:
3417 1585 513
BstEII G'GTnAC_C
Cuts at: 1184 2590 1184
Size: 1406 4109
BstYI r'GATC_y
Cuts at: 1972 2184 2356 2845 3212 3597 3614 5066 5152
Size: 212 172 489 367 385 17 1452 86
Cuts at: 5152 5164 5510 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 489 385 367 346 212 172
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2394 2394
Size: 5515
BtgI C'CryG_G
Cuts at: 513 1065 2004 2721 513
Size: 552 939 717 3307
Fragments arranged by size:
3307 939 717 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4776
Fragments arranged by size:
4776 683 56
BtsI GCAGTG_nn'
Cuts at: 3031 3825 3845 3031
Size: 794 20 4701
Fragments arranged by size:
4701 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2494 2819 2494
Size: 325 5190
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4522 809
Size: 507 3206 1802
Fragments arranged by size:
3206 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2962 3851 5480
Size: 54 1425 50 275 18 1132 889 1629
Cuts at: 5480 8
Size: 43
Fragments arranged by size:
1629 1425 1132 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 1537
Size: 275 1150 4090
Fragments arranged by size:
4090 1150 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2742 2750 3445 1353
Size: 1389 8 695 3423
Fragments arranged by size:
3423 1389 695 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4102 4476 4622 387
Size: 1064 138 2513 374 146 1280
Fragments arranged by size:
2513 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2408 3578 4175 4962 5230 1239
Size: 393 776 1170 597 787 268 1524
Fragments arranged by size:
1524 1170 787 776 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2128 2516 2525 2614 2785 2128
Size: 388 9 89 171 4858
Fragments arranged by size:
4858 388 171 89 9
EcoRI G'AATT_C
Cuts at: 2824 3202 2824
Size: 378 5137
FspI TGC'GCA
Cuts at: 4021 4021
Size: 5515
HaeII r_GCGC'y
Cuts at: 1678 4662 1678
Size: 2984 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4156 4188 4230 4262 1373
Size: 32 135 32 2584 32 42 32 2626
Fragments arranged by size:
2626 2584 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2713 2859 3105 669
Size: 988 281 775 146 246 3079
Fragments arranged by size:
3079 988 775 281 246 146
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5515
HpaI GTT'AAC
Cuts at: 3105 3105
Size: 5515
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 2530 3114 2530
Size: 584 4931
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4629 4813 1305
Size: 3324 184 2007
Fragments arranged by size:
3324 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3693
Fragments arranged by size:
3693 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2572 3473 3832 3991 518
Size: 648 1406 901 359 159 2042
Fragments arranged by size:
2042 1406 901 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2686 2764 2931 3608 4756 5001 1606
Size: 465 615 78 167 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 615 465 245 167 78
NcoI C'CATG_G
Cuts at: 513 1065 2004 2721 513
Size: 552 939 717 3307
Fragments arranged by size:
3307 939 717 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2873 4418 1132
Size: 97 1644 1545 2229
Fragments arranged by size:
2229 1644 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5515
PciI A'CATG_T
Cuts at: 4414 4414
Size: 5515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2360 1108
Size: 1252 4263
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PmlI CAC'GTG
Cuts at: 2410 2410
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 5097 5129 3584
Size: 32 1481 32 3970
Fragments arranged by size:
3970 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2128 2516 2785 2128
Size: 388 269 4858
Fragments arranged by size:
4858 388 269
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PspOMI G'GGCC_C
Cuts at: 2524 2524
Size: 5515
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2281 2313 2281
Size: 32 5483
PstI C_TGCA'G
Cuts at: 830 2199 2288 2622 2867 830
Size: 1369 89 334 245 3478
Fragments arranged by size:
3478 1369 334 245 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
PvuII CAG'CTG
Cuts at: 2071 2686 2764 2071
Size: 615 78 4822
Fragments arranged by size:
4822 615 78
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 3763 5475 3763
Size: 1712 3803
SfcI C'TryA_G
Cuts at: 826 1045 2046 2195 2284 2618 2863 3998 4679
Size: 219 1001 149 89 334 245 1135 681
Cuts at: 4679 4870 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1001 681 334 245 219 191
149 89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2842 1872
Size: 970 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2015 3619 4520 4782 820
Size: 197 926 72 1604 901 262 1553
Fragments arranged by size:
1604 1553 926 901 262 197 72
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2873 1132
Size: 1741 3774
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5515
StyI C'CwwG_G
Cuts at: 513 1065 2004 2520 2721 2954 513
Size: 552 939 516 201 233 3074
Fragments arranged by size:
3074 939 552 516 233 201
SwaI ATTT'AAAT
Cuts at: 2494 2494
Size: 5515
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3542 3559 3712 3897 4342 325
Size: 1255 1962 17 153 185 445 1498
Fragments arranged by size:
1962 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2172 2307 3761
Size: 274 80 33 51 1443 195 135 1454
Cuts at: 3761 5473 96
Size: 1712 138
Fragments arranged by size:
1712 1454 1443 274 195 138 135 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5475 5507 5475
Size: 32 5483
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5515
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5515
XmaI C'CCGG_G
Cuts at: 1870 2840 1870
Size: 970 4545
XmnI GAAnn'nnTTC
Cuts at: 2322 3644 2322
Size: 1322 4193
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PspXI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI XcmI