Restriction map for
pFN21AB6374
(Circular) (Six-base) MAPSORT of: pFN21AB6374.seq Check: 3792 from: 1 to: 5533
pFN21AB6374 5533 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3343 278
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2876 1937
Size: 939 4594
Acc65I G'GTAC_C
Cuts at: 2854 2854
Size: 5533
AclI AA'CG_TT
Cuts at: 1563 3660 4033 1563
Size: 2097 373 3063
Fragments arranged by size:
3063 2097 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2053 2268 2291 3596 4980 5248 1632
Size: 421 215 23 1305 1384 268 1917
Fragments arranged by size:
1917 1384 1305 421 268 215 23
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5336
AflIII A'CryG_T
Cuts at: 1203 2025 4432 5409 1203
Size: 822 2407 977 1327
Fragments arranged by size:
2407 1327 977 822
AgeI A'CCGG_T
Cuts at: 4364 4364
Size: 5533
AhdI GACnn_n'nnGTC
Cuts at: 4262 4262
Size: 5533
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5533
AlwNI CAG_nnn'CTG
Cuts at: 3276 4848 3276
Size: 1572 3961
ApaI G_GGCC'C
Cuts at: 2046 2055 2046
Size: 9 5524
ApaLI G'TGCA_C
Cuts at: 2687 3590 4746 2687
Size: 903 1156 3474
Fragments arranged by size:
3474 1156 903
ApoI r'AATT_y
Cuts at: 1493 2137 2842 3068 3220 5329 1493
Size: 644 705 226 152 2109 1697
Fragments arranged by size:
2109 1697 705 644 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4087 160
Size: 872 3055 1606
Fragments arranged by size:
3055 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 2047 2858 1870
Size: 73 95 9 811 4545
Fragments arranged by size:
4545 811 95 73 9
BamHI G'GATC_C
Cuts at: 2863 3230 2863
Size: 367 5166
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2854 4309 618
Size: 325 217 627 1067 1455 1842
Fragments arranged by size:
1842 1455 1067 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2046 2055 2852 1391
Size: 655 9 797 4072
Fragments arranged by size:
4072 797 655 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4562
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3724 3758 1903
Size: 34 1787 34 3678
Fragments arranged by size:
3678 1787 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2771 3425 4635 2771
Size: 654 1210 3669
Fragments arranged by size:
3669 1210 654
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5533
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2050 4144 136
Size: 107 122 71 631 605 378 2094 1525
Fragments arranged by size:
2094 1525 631 605 378 122 107 71
BglII A'GATC_T
Cuts at: 5528 5528
Size: 5533
BlpI GC'TnA_GC
Cuts at: 2950 2950
Size: 5533
Bme1580I G_kGCm'C
Cuts at: 1790 2046 2055 2691 3594 4750 1790
Size: 256 9 636 903 1156 2573
Fragments arranged by size:
2573 1156 903 636 256 9
BmgBI CAC'GTC
Cuts at: 1318 2088 2692 1318
Size: 770 604 4159
Fragments arranged by size:
4159 770 604
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3264 4222 442
Size: 416 53 83 399 24 1847 958 1753
Fragments arranged by size:
1847 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2597 4193 1239
Size: 1358 1596 2579
Fragments arranged by size:
2579 1596 1358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2155 3658 4523 4821 2155
Size: 1503 865 298 2867
Fragments arranged by size:
2867 1503 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2467 4196 882
Size: 617 968 1729 2219
Fragments arranged by size:
2219 1729 968 617
BsaAI yAC'GTr
Cuts at: 493 1204 3324 493
Size: 711 2120 2702
Fragments arranged by size:
2702 2120 711
BsaBI GATnn'nnATC
Cuts at: 2598 2759 5527 2598
Size: 161 2768 2604
Fragments arranged by size:
2768 2604 161
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3340 3722 4357 275
Size: 53 83 186 936 1807 382 635 1451
Fragments arranged by size:
1807 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2031 3966 4364 4638 4785 5196 2031
Size: 1935 398 274 147 411 2368
Fragments arranged by size:
2368 1935 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5308
Fragments arranged by size:
5308 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2465 2495 1186
Size: 1279 30 4224
Fragments arranged by size:
4224 1279 30
BseYI C'CCAG_C
Cuts at: 2055 4736 5232 2055
Size: 2681 496 2356
Fragments arranged by size:
2681 2356 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2110 2384 1163
Size: 573 374 274 4312
Fragments arranged by size:
4312 573 374 274
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2983 3744 4772 1540
Size: 275 186 982 761 1028 2301
Fragments arranged by size:
2301 1028 982 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2691 3594 3679 4750 2691
Size: 903 85 1071 3474
Fragments arranged by size:
3474 1071 903 85
BsmI GAATG_Cn'
Cuts at: 3044 3137 3044
Size: 93 5440
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2055 2691 2852 3594 3679 4750
Size: 399 256 9 636 161 742 85 1071
Cuts at: 4750 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 636 399 256 161 85
9
BspEI T'CCGG_A
Cuts at: 5196 5196
Size: 5533
BspHI T'CATG_A
Cuts at: 2322 3422 5152 2322
Size: 1100 1730 2703
Fragments arranged by size:
2703 1730 1100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2888 844
Size: 2044 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3420 1394
Size: 429 1597 3507
Fragments arranged by size:
3507 1597 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4028 4202 65
Size: 1183 2780 174 1396
Fragments arranged by size:
2780 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4177 4364 1700
Size: 110 2367 187 2869
Fragments arranged by size:
2869 2367 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5533
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5533
BssSI C'ACGA_G
Cuts at: 3593 4605 3593
Size: 1012 4521
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5533
BstBI TT'CG_AA
Cuts at: 4337 4337
Size: 5533
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5533
BstYI r'GATC_y
Cuts at: 1972 2417 2863 3230 3615 3632 5084 5170 5182
Size: 445 446 367 385 17 1452 86 12
Cuts at: 5182 5528 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 446 445 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4981
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4794
Fragments arranged by size:
4794 683 56
BtsI GCAGTG_nn'
Cuts at: 2121 3049 3843 3863 2121
Size: 928 794 20 3791
Fragments arranged by size:
3791 928 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5498
DraI TTT'AAA
Cuts at: 2611 2837 2611
Size: 226 5307
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4540 809
Size: 507 3224 1802
Fragments arranged by size:
3224 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2517 2619 2980
Size: 54 1425 50 275 18 687 102 361
Cuts at: 2980 3869 5498 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 687 361 275 102 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2980 1537
Size: 275 1168 4090
Fragments arranged by size:
4090 1168 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2430 3463 1353
Size: 1077 1033 3423
Fragments arranged by size:
3423 1077 1033
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4120 4494 4640 387
Size: 1064 138 2531 374 146 1280
Fragments arranged by size:
2531 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2268 2291 2597 3596 4193 4980
Size: 393 421 215 23 306 999 597 787
Cuts at: 4980 5248 1239
Size: 268 1524
Fragments arranged by size:
1524 999 787 597 421 393 306 268
215 23
EcoO109I rG'GnC_Cy
Cuts at: 2042 2043 2051 2052 2259 2042
Size: 1 8 1 207 5316
Fragments arranged by size:
5316 207 8 1 1
EcoRI G'AATT_C
Cuts at: 2842 3220 2842
Size: 378 5155
FspI TGC'GCA
Cuts at: 4039 4039
Size: 5533
HaeII r_GCGC'y
Cuts at: 1678 2296 4680 1678
Size: 618 2384 2531
Fragments arranged by size:
2531 2384 618
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2793 2825 4174 4206 4248
Size: 32 135 32 1221 32 1349 32 42
Cuts at: 4248 4280 1373
Size: 32 2626
Fragments arranged by size:
2626 1349 1221 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2877 3123 669
Size: 988 281 939 246 3079
Fragments arranged by size:
3079 988 939 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5533
HpaI GTT'AAC
Cuts at: 3123 3123
Size: 5533
KpnI G_GTAC'C
Cuts at: 2858 2858
Size: 5533
MfeI C'AATT_G
Cuts at: 3132 3132
Size: 5533
MluI A'CGCG_T
Cuts at: 5409 5409
Size: 5533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4647 4831 1305
Size: 3342 184 2007
Fragments arranged by size:
3342 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2519 2621 10
Size: 54 1425 343 687 102 2922
Fragments arranged by size:
2922 1425 687 343 102 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3491 3850 4009 518
Size: 648 2325 359 159 2042
Fragments arranged by size:
2325 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2094 2107 2458 2575 2949 3626 4774 5019
Size: 488 13 351 117 374 677 1148 245
Cuts at: 5019 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 488 374 351 245 117
13
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4981
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5533
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5533
NotI GC'GGCC_GC
Cuts at: 2980 2980
Size: 5533
NspI r_CATG'y
Cuts at: 1132 1229 2029 2891 4436 1132
Size: 97 800 862 1545 2229
Fragments arranged by size:
2229 1545 862 800 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5533
PciI A'CATG_T
Cuts at: 2025 4432 2025
Size: 2407 3126
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5533
PfoI T'CCnGG_A
Cuts at: 2061 2061
Size: 5533
PmeI GTTT'AAAC
Cuts at: 2837 2837
Size: 5533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 5115 5147 3602
Size: 32 1481 32 3988
Fragments arranged by size:
3988 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2259 2259
Size: 5533
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5533
PsiI TTA'TAA
Cuts at: 2823 3103 2823
Size: 280 5253
PspOMI G'GGCC_C
Cuts at: 2042 2051 2042
Size: 9 5524
PstI C_TGCA'G
Cuts at: 830 2341 2434 2885 830
Size: 1511 93 451 3478
Fragments arranged by size:
3478 1511 451 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5533
PvuII CAG'CTG
Cuts at: 2458 2575 2458
Size: 117 5416
SalI G'TCGA_C
Cuts at: 1936 2875 1936
Size: 939 4594
SbfI CC_TGCA'GG
Cuts at: 2885 2885
Size: 5533
ScaI AGT'ACT
Cuts at: 3781 5493 3781
Size: 1712 3821
SfcI C'TryA_G
Cuts at: 826 1045 2180 2337 2430 2881 4016 4697 4888
Size: 219 1135 157 93 451 1135 681 191
Cuts at: 4888 826
Size: 1471
Fragments arranged by size:
1471 1135 1135 681 451 219 191 157
93
SfiI GGCCn_nnn'nGGCC
Cuts at: 2050 2050
Size: 5533
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5533
SmaI CCC'GGG
Cuts at: 1872 2040 2860 1872
Size: 168 820 4545
Fragments arranged by size:
4545 820 168
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2170 3637 4538 4800 820
Size: 197 926 227 1467 901 262 1553
Fragments arranged by size:
1553 1467 926 901 262 227 197
SnaBI TAC'GTA
Cuts at: 493 3324 493
Size: 2831 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5533
SphI G_CATG'C
Cuts at: 1132 2891 1132
Size: 1759 3774
SspI AAT'ATT
Cuts at: 5 52 3457 5
Size: 47 3405 2081
Fragments arranged by size:
3405 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5533
StyI C'CwwG_G
Cuts at: 513 1065 2226 2972 513
Size: 552 1161 746 3074
Fragments arranged by size:
3074 1161 746 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3560 3577 3730 3915 4360 325
Size: 1255 1980 17 153 185 445 1498
Fragments arranged by size:
1980 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3779 5491 96
Size: 274 80 33 51 1443 1802 1712 138
Fragments arranged by size:
1802 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5493 5525 5493
Size: 32 5501
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5533
XbaI T'CTAG_A
Cuts at: 2869 2869
Size: 5533
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5533
XmaI C'CCGG_G
Cuts at: 1870 2038 2858 1870
Size: 168 820 4545
Fragments arranged by size:
4545 820 168
XmnI GAAnn'nnTTC
Cuts at: 2330 3662 2330
Size: 1332 4201
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3341 276
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfoI
SrfI SwaI XcmI