Restriction map for
pFN21AB6369
(Circular) (Six-base) MAPSORT of: pFN21AB6369.seq Check: 9409 from: 1 to: 5512
pFN21AB6369 5512 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3322 278
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2855 1937
Size: 918 4594
Acc65I G'GTAC_C
Cuts at: 2833 2833
Size: 5512
AclI AA'CG_TT
Cuts at: 1563 3639 4012 1563
Size: 2076 373 3063
Fragments arranged by size:
3063 2076 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2085 2280 3575 4959 5227 1632
Size: 453 195 1295 1384 268 1917
Fragments arranged by size:
1917 1384 1295 453 268 195
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5315
AflIII A'CryG_T
Cuts at: 1203 4411 5388 1203
Size: 3208 977 1327
Fragments arranged by size:
3208 1327 977
AgeI A'CCGG_T
Cuts at: 4343 4343
Size: 5512
AhdI GACnn_n'nnGTC
Cuts at: 4241 4241
Size: 5512
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5512
AlwNI CAG_nnn'CTG
Cuts at: 3255 4827 3255
Size: 1572 3940
ApaLI G'TGCA_C
Cuts at: 3569 4725 3569
Size: 1156 4356
ApoI r'AATT_y
Cuts at: 1493 2821 3047 3199 5308 1493
Size: 1328 226 152 2109 1697
Fragments arranged by size:
2109 1697 1328 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4066 160
Size: 872 3034 1606
Fragments arranged by size:
3034 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
AvaI C'yCGr_G
Cuts at: 1870 1943 2837 1870
Size: 73 894 4545
Fragments arranged by size:
4545 894 73
BamHI G'GATC_C
Cuts at: 2842 3209 2842
Size: 367 5145
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2833 4288 618
Size: 325 217 627 1046 1455 1842
Fragments arranged by size:
1842 1455 1046 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2831 1391
Size: 1440 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2268 2370 2565 928
Size: 971 369 102 195 3875
Fragments arranged by size:
3875 971 369 195 102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2147 2181 3703 3737 1903
Size: 34 210 34 1522 34 3678
Fragments arranged by size:
3678 1522 210 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3404 4614 3404
Size: 1210 4302
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5512
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4123 136
Size: 107 122 71 631 605 2451 1525
Fragments arranged by size:
2451 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2311 5507 2311
Size: 3196 2316
BlpI GC'TnA_GC
Cuts at: 2169 2929 2169
Size: 760 4752
Bme1580I G_kGCm'C
Cuts at: 1790 3573 4729 1790
Size: 1783 1156 2573
Fragments arranged by size:
2573 1783 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5512
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3243 4201 442
Size: 416 53 83 399 24 1826 958 1753
Fragments arranged by size:
1826 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4172 1239
Size: 2933 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2554 2782 3637 4502 4800 2554
Size: 228 855 865 298 3266
Fragments arranged by size:
3266 865 855 298 228
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4175 882
Size: 617 2676 2219
Fragments arranged by size:
2676 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3303 493
Size: 711 2099 2702
Fragments arranged by size:
2702 2099 711
BsaBI GATnn'nnATC
Cuts at: 2022 5506 2022
Size: 3484 2028
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3319 3701 4336 275
Size: 53 83 186 936 1786 382 635 1451
Fragments arranged by size:
1786 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2026 3945 4343 4617 4764 5175 2026
Size: 1919 398 274 147 411 2363
Fragments arranged by size:
2363 1919 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5287
Fragments arranged by size:
5287 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5512
BseYI C'CCAG_C
Cuts at: 4715 5211 4715
Size: 496 5016
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4939
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2962 3723 4751 1540
Size: 275 186 961 761 1028 2301
Fragments arranged by size:
2301 1028 961 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3573 3658 4729 3573
Size: 85 1071 4356
Fragments arranged by size:
4356 1071 85
BsmI GAATG_Cn'
Cuts at: 3023 3116 3023
Size: 93 5419
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2831 3573 3658 4729 1391
Size: 399 1041 742 85 1071 2174
Fragments arranged by size:
2174 1071 1041 742 399 85
BspEI T'CCGG_A
Cuts at: 2026 5175 2026
Size: 3149 2363
BspHI T'CATG_A
Cuts at: 3401 5131 3401
Size: 1730 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2867 844
Size: 2023 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3399 1394
Size: 429 1576 3507
Fragments arranged by size:
3507 1576 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4007 4181 65
Size: 1183 2759 174 1396
Fragments arranged by size:
2759 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4156 4343 1700
Size: 110 2346 187 2869
Fragments arranged by size:
2869 2346 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5512
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5512
BssSI C'ACGA_G
Cuts at: 3572 4584 3572
Size: 1012 4500
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5512
BstBI TT'CG_AA
Cuts at: 4316 4316
Size: 5512
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5512
BstYI r'GATC_y
Cuts at: 1972 2017 2023 2311 2776 2842 3209 3594 3611
Size: 45 6 288 465 66 367 385 17
Cuts at: 3611 5063 5149 5161 5507 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 465 385 367 346 288 86
66 45 17 12 6
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4960
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4773
Fragments arranged by size:
4773 683 56
BtsI GCAGTG_nn'
Cuts at: 2636 3028 3822 3842 2636
Size: 392 794 20 4306
Fragments arranged by size:
4306 794 392 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5512
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5477
DraI TTT'AAA
Cuts at: 2816 2816
Size: 5512
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4519 809
Size: 507 3203 1802
Fragments arranged by size:
3203 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2720 2959 3848
Size: 54 1425 50 275 18 890 239 889
Cuts at: 3848 5477 8
Size: 1629 43
Fragments arranged by size:
1629 1425 890 889 275 239 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2959 1537
Size: 275 1147 4090
Fragments arranged by size:
4090 1147 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2283 3442 1353
Size: 930 1159 3423
Fragments arranged by size:
3423 1159 930
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4099 4473 4619 387
Size: 1064 138 2510 374 146 1280
Fragments arranged by size:
2510 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2085 2280 3575 4172 4959 5227 1239
Size: 393 453 195 1295 597 787 268 1524
Fragments arranged by size:
1524 1295 787 597 453 393 268 195
EcoRI G'AATT_C
Cuts at: 2821 3199 2821
Size: 378 5134
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2090 2383 2090
Size: 293 5219
FspI TGC'GCA
Cuts at: 4018 4018
Size: 5512
HaeII r_GCGC'y
Cuts at: 1678 4659 1678
Size: 2981 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2296 2328 4153 4185 4227
Size: 32 135 32 724 32 1825 32 42
Cuts at: 4227 4259 1373
Size: 32 2626
Fragments arranged by size:
2626 1825 724 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2856 3102 669
Size: 988 281 918 246 3079
Fragments arranged by size:
3079 988 918 281 246
HindIII A'AGCT_T
Cuts at: 748 2799 748
Size: 2051 3461
HpaI GTT'AAC
Cuts at: 3102 3102
Size: 5512
KpnI G_GTAC'C
Cuts at: 2837 2837
Size: 5512
MfeI C'AATT_G
Cuts at: 3111 3111
Size: 5512
MluI A'CGCG_T
Cuts at: 5388 5388
Size: 5512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2370 2804 4626 4810 1305
Size: 1065 434 1822 184 2007
Fragments arranged by size:
2007 1822 1065 434 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2722 10
Size: 54 1425 343 890 2800
Fragments arranged by size:
2800 1425 890 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3470 3829 3988 518
Size: 648 2304 359 159 2042
Fragments arranged by size:
2304 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2210 2928 3605 4753 4998 1606
Size: 604 718 677 1148 245 2120
Fragments arranged by size:
2120 1148 718 677 604 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4960
NdeI CA'TA_TG
Cuts at: 387 2695 387
Size: 2308 3204
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5512
NotI GC'GGCC_GC
Cuts at: 2959 2959
Size: 5512
NspI r_CATG'y
Cuts at: 1132 1229 2870 4415 1132
Size: 97 1641 1545 2229
Fragments arranged by size:
2229 1641 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5512
PciI A'CATG_T
Cuts at: 4411 4411
Size: 5512
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5512
PmeI GTTT'AAAC
Cuts at: 2816 2816
Size: 5512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3581 3613 5094 5126 3581
Size: 32 1481 32 3967
Fragments arranged by size:
3967 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5512
PsiI TTA'TAA
Cuts at: 2663 3082 2663
Size: 419 5093
PstI C_TGCA'G
Cuts at: 830 2864 830
Size: 2034 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5512
SalI G'TCGA_C
Cuts at: 1936 2854 1936
Size: 918 4594
SbfI CC_TGCA'GG
Cuts at: 2864 2864
Size: 5512
ScaI AGT'ACT
Cuts at: 2035 3760 5472 2035
Size: 1725 1712 2075
Fragments arranged by size:
2075 1725 1712
SfcI C'TryA_G
Cuts at: 826 1045 2037 2860 3995 4676 4867 826
Size: 219 992 823 1135 681 191 1471
Fragments arranged by size:
1471 1135 992 823 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5512
SmaI CCC'GGG
Cuts at: 1872 2839 1872
Size: 967 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2533 2761 3616 4517 4779 820
Size: 197 926 590 228 855 901 262 1553
Fragments arranged by size:
1553 926 901 855 590 262 228 197
SnaBI TAC'GTA
Cuts at: 493 3303 493
Size: 2810 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5512
SphI G_CATG'C
Cuts at: 1132 2870 1132
Size: 1738 3774
SspI AAT'ATT
Cuts at: 5 52 3436 5
Size: 47 3384 2081
Fragments arranged by size:
3384 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5512
StyI C'CwwG_G
Cuts at: 513 1065 2541 2951 513
Size: 552 1476 410 3074
Fragments arranged by size:
3074 1476 552 410
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3539 3556 3709 3894 4339 325
Size: 1255 1959 17 153 185 445 1498
Fragments arranged by size:
1959 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2033 2425 3758
Size: 274 80 33 51 1443 56 392 1333
Cuts at: 3758 5470 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1333 392 274 138 80 56
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2369 2401 5472 5504 2369
Size: 32 3071 32 2377
Fragments arranged by size:
3071 2377 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5512
XbaI T'CTAG_A
Cuts at: 2020 2554 2848 2020
Size: 534 294 4684
Fragments arranged by size:
4684 534 294
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5512
XmaI C'CCGG_G
Cuts at: 1870 2837 1870
Size: 967 4545
XmnI GAAnn'nnTTC
Cuts at: 2247 2265 3641 2247
Size: 18 1376 4118
Fragments arranged by size:
4118 1376 18
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3320 276
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI