Restriction map for
pFN21AB6368
(Circular) (Six-base) MAPSORT of: pFN21AB6368.seq Check: 3015 from: 1 to: 5599
pFN21AB6368 5599 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2194 2194
Size: 5599
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3409 278
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2484 2942 1937
Size: 547 458 4594
Fragments arranged by size:
4594 547 458
Acc65I G'GTAC_C
Cuts at: 2920 2920
Size: 5599
AclI AA'CG_TT
Cuts at: 1563 3726 4099 1563
Size: 2163 373 3063
Fragments arranged by size:
3063 2163 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2244 3662 5046 5314 1632
Size: 612 1418 1384 268 1917
Fragments arranged by size:
1917 1418 1384 612 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5402
AflIII A'CryG_T
Cuts at: 1203 4498 5475 1203
Size: 3295 977 1327
Fragments arranged by size:
3295 1327 977
AgeI A'CCGG_T
Cuts at: 4430 4430
Size: 5599
AhdI GACnn_n'nnGTC
Cuts at: 4328 4328
Size: 5599
AleI CACnn'nnGTG
Cuts at: 1166 2393 1166
Size: 1227 4372
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2081 2555 1184
Size: 897 474 4228
Fragments arranged by size:
4228 897 474
AlwNI CAG_nnn'CTG
Cuts at: 3342 4914 3342
Size: 1572 4027
ApaLI G'TGCA_C
Cuts at: 3656 4812 3656
Size: 1156 4443
ApoI r'AATT_y
Cuts at: 1493 2663 2908 3134 3286 5395 1493
Size: 1170 245 226 152 2109 1697
Fragments arranged by size:
2109 1697 1170 245 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4153 160
Size: 872 3121 1606
Fragments arranged by size:
3121 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
AvaI C'yCGr_G
Cuts at: 1870 1943 2924 1870
Size: 73 981 4545
Fragments arranged by size:
4545 981 73
BamHI G'GATC_C
Cuts at: 2929 3296 2929
Size: 367 5232
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2920 4375 618
Size: 325 217 627 1133 1455 1842
Fragments arranged by size:
1842 1455 1133 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2529 2709 2918 1391
Size: 1138 180 209 4072
Fragments arranged by size:
4072 1138 209 180
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2196 2288 2388 928
Size: 971 297 92 100 4139
Fragments arranged by size:
4139 971 297 100 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3790 3824 1903
Size: 34 1853 34 3678
Fragments arranged by size:
3678 1853 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3491 4701 3491
Size: 1210 4389
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5599
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4210 136
Size: 107 122 71 631 605 2538 1525
Fragments arranged by size:
2538 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5594 5594
Size: 5599
BlpI GC'TnA_GC
Cuts at: 3016 3016
Size: 5599
Bme1580I G_kGCm'C
Cuts at: 1790 2571 2857 3660 4816 1790
Size: 781 286 803 1156 2573
Fragments arranged by size:
2573 1156 803 781 286
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5599
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3330 4288 442
Size: 416 53 83 399 24 1913 958 1753
Fragments arranged by size:
1913 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5599
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 5599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2292 2458 4259 1239
Size: 1053 166 1801 2579
Fragments arranged by size:
2579 1801 1053 166
Bpu10I CC'TnA_GC
Cuts at: 2102 2102
Size: 5599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2843 3724 4589 4887 2843
Size: 881 865 298 3555
Fragments arranged by size:
3555 881 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4262 882
Size: 617 2763 2219
Fragments arranged by size:
2763 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3390 493
Size: 711 2186 2702
Fragments arranged by size:
2702 2186 711
BsaBI GATnn'nnATC
Cuts at: 5593 5593
Size: 5599
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3406 3788 4423 275
Size: 53 83 186 936 1873 382 635 1451
Fragments arranged by size:
1873 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4032 4430 4704 4851 5262 4032
Size: 398 274 147 411 4369
Fragments arranged by size:
4369 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2334 2364 1178
Size: 30 165 30 931 30 4413
Fragments arranged by size:
4413 931 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5599
BseYI C'CCAG_C
Cuts at: 2127 2140 2338 2770 2799 4802 5298 2127
Size: 13 198 432 29 2003 496 2428
Fragments arranged by size:
2428 2003 496 432 198 29 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2027 1163
Size: 573 291 4735
Fragments arranged by size:
4735 573 291
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2786 3049 3810 4838 1540
Size: 275 186 785 263 761 1028 2301
Fragments arranged by size:
2301 1028 785 761 275 263 186
BsiHKAI G_wGCw'C
Cuts at: 2108 2529 2709 2898 3660 3745 4816 2108
Size: 421 180 189 762 85 1071 2891
Fragments arranged by size:
2891 1071 762 421 189 180 85
BsmI GAATG_Cn'
Cuts at: 3110 3203 3110
Size: 93 5506
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2529 2571 2709 2857 2898 2918
Size: 399 318 421 42 138 148 41 20
Cuts at: 2918 3660 3745 4816 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 421 399 318 148 138
85 42 41 20
BspEI T'CCGG_A
Cuts at: 5262 5262
Size: 5599
BspHI T'CATG_A
Cuts at: 3488 5218 3488
Size: 1730 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2194 2954 844
Size: 1350 760 3489
Fragments arranged by size:
3489 1350 760
BsrBI CCG'CTC
Cuts at: 1394 1823 2868 3486 1394
Size: 429 1045 618 3507
Fragments arranged by size:
3507 1045 618 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4094 4268 65
Size: 1183 2846 174 1396
Fragments arranged by size:
2846 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2046 2785 4243 4430 1700
Size: 110 236 739 1458 187 2869
Fragments arranged by size:
2869 1458 739 236 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5599
BssHII G'CGCG_C
Cuts at: 1925 2803 1925
Size: 878 4721
BssSI C'ACGA_G
Cuts at: 2579 3659 4671 2579
Size: 1080 1012 3507
Fragments arranged by size:
3507 1080 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5599
BstBI TT'CG_AA
Cuts at: 4403 4403
Size: 5599
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5599
BstXI CCAn_nnnn'nTGG
Cuts at: 2093 2199 2395 2093
Size: 106 196 5297
Fragments arranged by size:
5297 196 106
BstYI r'GATC_y
Cuts at: 1972 2266 2929 3296 3681 3698 5150 5236 5248
Size: 294 663 367 385 17 1452 86 12
Cuts at: 5248 5594 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 663 385 367 346 294 86
17 12
BstZ17I GTA'TAC
Cuts at: 2485 2485
Size: 5599
BtgI C'CryG_G
Cuts at: 513 1065 2078 513
Size: 552 1013 4034
Fragments arranged by size:
4034 1013 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2476 487
Size: 683 56 1250 3610
Fragments arranged by size:
3610 1250 683 56
BtsI GCAGTG_nn'
Cuts at: 2063 2473 3115 3909 3929 2063
Size: 410 642 794 20 3733
Fragments arranged by size:
3733 794 642 410 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5564
DraI TTT'AAA
Cuts at: 2903 2903
Size: 5599
DraIII CAC_nnn'GTG
Cuts at: 2698 2698
Size: 5599
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4606 809
Size: 507 3290 1802
Fragments arranged by size:
3290 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2095 2292 2700
Size: 54 1425 50 275 18 265 197 408
Cuts at: 2700 2757 2783 2787 2889 3046 3935 5564 8
Size: 57 26 4 102 157 889 1629 43
Fragments arranged by size:
1629 1425 889 408 275 265 197 157
102 57 54 50 43 26 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2783 3046 1537
Size: 275 971 263 4090
Fragments arranged by size:
4090 971 275 263
EarI CTCTTCn'nnn_
Cuts at: 1353 2622 2714 3529 1353
Size: 1269 92 815 3423
Fragments arranged by size:
3423 1269 815 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2668 4186 4560 4706 387
Size: 1064 138 1079 1518 374 146 1280
Fragments arranged by size:
1518 1280 1079 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2527 2707 2527
Size: 180 5419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2244 2292 2458 3662 4259 5046 5314
Size: 393 612 48 166 1204 597 787 268
Cuts at: 5314 1239
Size: 1524
Fragments arranged by size:
1524 1204 787 612 597 393 268 166
48
EcoO109I rG'GnC_Cy
Cuts at: 2323 2361 2323
Size: 38 5561
EcoRI G'AATT_C
Cuts at: 2908 3286 2908
Size: 378 5221
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2305 2614 2305
Size: 309 5290
FseI GG_CCGG'CC
Cuts at: 2789 2789
Size: 5599
FspI TGC'GCA
Cuts at: 4105 4105
Size: 5599
HaeII r_GCGC'y
Cuts at: 1678 4746 1678
Size: 3068 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 4240 4272 4314
Size: 32 135 32 614 32 2022 32 42
Cuts at: 4314 4346 1373
Size: 32 2626
Fragments arranged by size:
2626 2022 614 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2943 3189 669
Size: 988 281 1005 246 3079
Fragments arranged by size:
3079 1005 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5599
HpaI GTT'AAC
Cuts at: 3189 3189
Size: 5599
KpnI G_GTAC'C
Cuts at: 2924 2924
Size: 5599
MfeI C'AATT_G
Cuts at: 3198 3198
Size: 5599
MluI A'CGCG_T
Cuts at: 5475 5475
Size: 5599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4713 4897 1305
Size: 3408 184 2007
Fragments arranged by size:
3408 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2294 10
Size: 54 1425 343 462 3315
Fragments arranged by size:
3315 1425 462 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2393 2579 3557 3916 4075 518
Size: 648 1227 186 978 359 159 2042
Fragments arranged by size:
2042 1227 978 648 359 186 159
MspA1I CmG'CkG
Cuts at: 1606 2338 2539 2607 3015 3692 4840 5085 1606
Size: 732 201 68 408 677 1148 245 2120
Fragments arranged by size:
2120 1148 732 677 408 245 201 68
NaeI GCC'GGC
Cuts at: 2787 2787
Size: 5599
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5047
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5599
NgoMIV G'CCGG_C
Cuts at: 2785 2785
Size: 5599
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5599
NotI GC'GGCC_GC
Cuts at: 3046 3046
Size: 5599
NspI r_CATG'y
Cuts at: 1132 1229 2957 4502 1132
Size: 97 1728 1545 2229
Fragments arranged by size:
2229 1728 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5599
PciI A'CATG_T
Cuts at: 4498 4498
Size: 5599
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2887 1108
Size: 1779 3820
PfoI T'CCnGG_A
Cuts at: 2269 2269
Size: 5599
PmeI GTTT'AAAC
Cuts at: 2903 2903
Size: 5599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3668 3700 5181 5213 3668
Size: 32 1481 32 4054
Fragments arranged by size:
4054 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2323 2361 2323
Size: 38 5561
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5599
PsiI TTA'TAA
Cuts at: 3169 3169
Size: 5599
PstI C_TGCA'G
Cuts at: 830 2460 2691 2951 830
Size: 1630 231 260 3478
Fragments arranged by size:
3478 1630 260 231
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5599
PvuII CAG'CTG
Cuts at: 2338 2539 2338
Size: 201 5398
SacI G_AGCT'C
Cuts at: 2529 2709 2529
Size: 180 5419
SalI G'TCGA_C
Cuts at: 1936 2941 1936
Size: 1005 4594
SapI GCTCTTCn'nnn_
Cuts at: 2714 2714
Size: 5599
SbfI CC_TGCA'GG
Cuts at: 2460 2951 2460
Size: 491 5108
ScaI AGT'ACT
Cuts at: 3847 5559 3847
Size: 1712 3887
SfcI C'TryA_G
Cuts at: 826 1045 2456 2687 2947 4082 4763 4954 826
Size: 219 1411 231 260 1135 681 191 1471
Fragments arranged by size:
1471 1411 1135 681 260 231 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
SgrAI Cr'CCGG_yG
Cuts at: 1700 2046 1700
Size: 346 5253
SmaI CCC'GGG
Cuts at: 1872 2926 1872
Size: 1054 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2858 3703 4604 4866 820
Size: 197 926 915 845 901 262 1553
Fragments arranged by size:
1553 926 915 901 845 262 197
SnaBI TAC'GTA
Cuts at: 493 3390 493
Size: 2897 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5599
SphI G_CATG'C
Cuts at: 1132 2957 1132
Size: 1825 3774
SspI AAT'ATT
Cuts at: 5 52 3523 5
Size: 47 3471 2081
Fragments arranged by size:
3471 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5599
StyI C'CwwG_G
Cuts at: 513 1065 2760 3038 513
Size: 552 1695 278 3074
Fragments arranged by size:
3074 1695 552 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2186 2514 3626 3643 3796 3981 4426
Size: 1255 606 328 1112 17 153 185 445
Cuts at: 4426 325
Size: 1498
Fragments arranged by size:
1498 1255 1112 606 445 328 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3845 5557 96
Size: 274 80 33 51 1443 1868 1712 138
Fragments arranged by size:
1868 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5559 5591 5559
Size: 32 5567
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5599
XbaI T'CTAG_A
Cuts at: 2935 2935
Size: 5599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2578 2578
Size: 5599
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5599
XmaI C'CCGG_G
Cuts at: 1870 2924 1870
Size: 1054 4545
XmnI GAAnn'nnTTC
Cuts at: 3728 3728
Size: 5599
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3407 276
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI Bsu36I EcoNI EcoRV FspAI KasI
NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI