Restriction map for
pFN21AB6365
(Circular) (Six-base) MAPSORT of: pFN21AB6365.seq Check: 3012 from: 1 to: 5509
pFN21AB6365 5509 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3319 278
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2852 1937
Size: 915 4594
Acc65I G'GTAC_C
Cuts at: 2830 2830
Size: 5509
AclI AA'CG_TT
Cuts at: 1563 3636 4009 1563
Size: 2073 373 3063
Fragments arranged by size:
3063 2073 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2020 3572 4956 5224 1632
Size: 388 1552 1384 268 1917
Fragments arranged by size:
1917 1552 1384 388 268
AfeI AGC'GCT
Cuts at: 2161 2161
Size: 5509
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5312
AflIII A'CryG_T
Cuts at: 1203 4408 5385 1203
Size: 3205 977 1327
Fragments arranged by size:
3205 1327 977
AgeI A'CCGG_T
Cuts at: 4340 4340
Size: 5509
AhdI GACnn_n'nnGTC
Cuts at: 4238 4238
Size: 5509
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5509
AlwNI CAG_nnn'CTG
Cuts at: 2058 3252 4824 2058
Size: 1194 1572 2743
Fragments arranged by size:
2743 1572 1194
ApaLI G'TGCA_C
Cuts at: 3566 4722 3566
Size: 1156 4353
ApoI r'AATT_y
Cuts at: 1493 2423 2818 3044 3196 5305 1493
Size: 930 395 226 152 2109 1697
Fragments arranged by size:
2109 1697 930 395 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4063 160
Size: 872 3031 1606
Fragments arranged by size:
3031 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
AvaI C'yCGr_G
Cuts at: 1870 1943 2254 2616 2834 1870
Size: 73 311 362 218 4545
Fragments arranged by size:
4545 362 311 218 73
BamHI G'GATC_C
Cuts at: 2336 2839 3206 2336
Size: 503 367 4639
Fragments arranged by size:
4639 503 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2259 2830 4285 618
Size: 325 217 627 472 571 1455 1842
Fragments arranged by size:
1842 1455 627 571 472 325 217
BanII G_rGCy'C
Cuts at: 1391 2065 2252 2828 1391
Size: 674 187 576 4072
Fragments arranged by size:
4072 674 576 187
BbeI G_GCGC'C
Cuts at: 2263 2263
Size: 5509
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2160 928
Size: 971 261 4277
Fragments arranged by size:
4277 971 261
BbvCI CC'TCA_GC
Cuts at: 2051 2530 2051
Size: 479 5030
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3700 3734 1903
Size: 34 1763 34 3678
Fragments arranged by size:
3678 1763 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3401 4611 3401
Size: 1210 4299
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5509
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2268 4120 136
Size: 107 122 71 631 605 596 1852 1525
Fragments arranged by size:
1852 1525 631 605 596 122 107 71
BglII A'GATC_T
Cuts at: 2310 5504 2310
Size: 3194 2315
BlpI GC'TnA_GC
Cuts at: 2926 2926
Size: 5509
Bme1580I G_kGCm'C
Cuts at: 1790 3570 4726 1790
Size: 1780 1156 2573
Fragments arranged by size:
2573 1780 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5509
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3240 4198 442
Size: 416 53 83 399 24 1823 958 1753
Fragments arranged by size:
1823 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2119 4169 1239
Size: 880 2050 2579
Fragments arranged by size:
2579 2050 880
Bpu10I CC'TnA_GC
Cuts at: 2051 2530 2051
Size: 479 5030
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2777 3634 4499 4797 2777
Size: 857 865 298 3489
Fragments arranged by size:
3489 865 857 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4172 882
Size: 617 2673 2219
Fragments arranged by size:
2673 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3300 493
Size: 711 2096 2702
Fragments arranged by size:
2702 2096 711
BsaBI GATnn'nnATC
Cuts at: 5503 5503
Size: 5509
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2260 3316 3698 4333
Size: 53 83 186 936 727 1056 382 635
Cuts at: 4333 275
Size: 1451
Fragments arranged by size:
1451 1056 936 727 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3942 4340 4614 4761 5172 3942
Size: 398 274 147 411 4279
Fragments arranged by size:
4279 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5284
Fragments arranged by size:
5284 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 2015 2018 2173 2176 2421 1186
Size: 826 3 3 155 3 245 4274
Fragments arranged by size:
4274 826 245 155 3 3 3
BseYI C'CCAG_C
Cuts at: 2628 4712 5208 2628
Size: 2084 496 2929
Fragments arranged by size:
2929 2084 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2629 2739 1163
Size: 573 893 110 3933
Fragments arranged by size:
3933 893 573 110
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2959 3720 4748 1540
Size: 275 186 958 761 1028 2301
Fragments arranged by size:
2301 1028 958 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3570 3655 4726 3570
Size: 85 1071 4353
Fragments arranged by size:
4353 1071 85
BsmI GAATG_Cn'
Cuts at: 3020 3113 3020
Size: 93 5416
BsmBI CGTCTCn'nnnn_
Cuts at: 2084 2084
Size: 5509
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2065 2252 2828 3570 3655 4726 1391
Size: 399 275 187 576 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 576 399 275 187 85
BspEI T'CCGG_A
Cuts at: 5172 5172
Size: 5509
BspHI T'CATG_A
Cuts at: 3398 5128 3398
Size: 1730 3779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2864 844
Size: 2020 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3396 1394
Size: 429 1573 3507
Fragments arranged by size:
3507 1573 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4004 4178 65
Size: 1183 2756 174 1396
Fragments arranged by size:
2756 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2267 4153 4340 1700
Size: 110 457 1886 187 2869
Fragments arranged by size:
2869 1886 457 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5509
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5509
BssSI C'ACGA_G
Cuts at: 3569 4581 3569
Size: 1012 4497
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5509
BstBI TT'CG_AA
Cuts at: 4313 4313
Size: 5509
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5509
BstXI CCAn_nnnn'nTGG
Cuts at: 2219 2219
Size: 5509
BstYI r'GATC_y
Cuts at: 1972 2310 2336 2839 3206 3591 3608 5060 5146
Size: 338 26 503 367 385 17 1452 86
Cuts at: 5146 5158 5504 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 503 385 367 346 338 86
26 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4957
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2447 487
Size: 683 56 1221 3549
Fragments arranged by size:
3549 1221 683 56
BtsI GCAGTG_nn'
Cuts at: 3025 3819 3839 3025
Size: 794 20 4695
Fragments arranged by size:
4695 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5509
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5474
DraI TTT'AAA
Cuts at: 2813 2813
Size: 5509
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4516 809
Size: 507 3200 1802
Fragments arranged by size:
3200 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2956 3845 5474
Size: 54 1425 50 275 18 1126 889 1629
Cuts at: 5474 8
Size: 43
Fragments arranged by size:
1629 1425 1126 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2956 1537
Size: 275 1144 4090
Fragments arranged by size:
4090 1144 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2036 2179 3439 1353
Size: 683 143 1260 3423
Fragments arranged by size:
3423 1260 683 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4096 4470 4616 387
Size: 1064 138 2507 374 146 1280
Fragments arranged by size:
2507 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2119 3572 4169 4956 5224 1239
Size: 393 388 99 1453 597 787 268 1524
Fragments arranged by size:
1524 1453 787 597 393 388 268 99
EcoRI G'AATT_C
Cuts at: 2818 3196 2818
Size: 378 5131
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2818 2818
Size: 5509
FspI TGC'GCA
Cuts at: 4015 4015
Size: 5509
HaeII r_GCGC'y
Cuts at: 1678 2058 2163 2263 4656 1678
Size: 380 105 100 2393 2531
Fragments arranged by size:
2531 2393 380 105 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2277 2309 4150 4182 4224
Size: 32 135 32 705 32 1841 32 42
Cuts at: 4224 4256 1373
Size: 32 2626
Fragments arranged by size:
2626 1841 705 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2853 3099 669
Size: 988 281 915 246 3079
Fragments arranged by size:
3079 988 915 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5509
HpaI GTT'AAC
Cuts at: 3099 3099
Size: 5509
KasI G'GCGC_C
Cuts at: 2259 2259
Size: 5509
KpnI G_GTAC'C
Cuts at: 2834 2834
Size: 5509
MfeI C'AATT_G
Cuts at: 3108 3108
Size: 5509
MluI A'CGCG_T
Cuts at: 5385 5385
Size: 5509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2649 4623 4807 1305
Size: 1344 1974 184 2007
Fragments arranged by size:
2007 1974 1344 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3687
Fragments arranged by size:
3687 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2460 3467 3826 3985 518
Size: 648 1294 1007 359 159 2042
Fragments arranged by size:
2042 1294 1007 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2108 2925 3602 4750 4995 1606
Size: 502 817 677 1148 245 2120
Fragments arranged by size:
2120 1148 817 677 502 245
NarI GG'CG_CC
Cuts at: 2260 2260
Size: 5509
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4957
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5509
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5509
NotI GC'GGCC_GC
Cuts at: 2956 2956
Size: 5509
NspI r_CATG'y
Cuts at: 1132 1229 2867 4412 1132
Size: 97 1638 1545 2229
Fragments arranged by size:
2229 1638 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5509
PciI A'CATG_T
Cuts at: 4408 4408
Size: 5509
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5509
PmeI GTTT'AAAC
Cuts at: 2813 2813
Size: 5509
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3578 3610 5091 5123 3578
Size: 32 1481 32 3964
Fragments arranged by size:
3964 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5509
PsiI TTA'TAA
Cuts at: 3079 3079
Size: 5509
PstI C_TGCA'G
Cuts at: 830 2285 2310 2861 830
Size: 1455 25 551 3478
Fragments arranged by size:
3478 1455 551 25
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5509
RsrII CG'GwC_CG
Cuts at: 2265 2265
Size: 5509
SalI G'TCGA_C
Cuts at: 1936 2851 1936
Size: 915 4594
SbfI CC_TGCA'GG
Cuts at: 2861 2861
Size: 5509
ScaI AGT'ACT
Cuts at: 3757 5469 3757
Size: 1712 3797
SfcI C'TryA_G
Cuts at: 826 1045 2281 2306 2857 3992 4673 4864 826
Size: 219 1236 25 551 1135 681 191 1471
Fragments arranged by size:
1471 1236 1135 681 551 219 191 25
SfoI GGC'GCC
Cuts at: 2261 2261
Size: 5509
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5509
SmaI CCC'GGG
Cuts at: 1872 2618 2836 1872
Size: 746 218 4545
Fragments arranged by size:
4545 746 218
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2792 3613 4514 4776 820
Size: 197 926 849 821 901 262 1553
Fragments arranged by size:
1553 926 901 849 821 262 197
SnaBI TAC'GTA
Cuts at: 493 3300 493
Size: 2807 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5509
SphI G_CATG'C
Cuts at: 1132 2867 1132
Size: 1735 3774
SspI AAT'ATT
Cuts at: 5 52 2373 3433 5
Size: 47 2321 1060 2081
Fragments arranged by size:
2321 2081 1060 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5509
StyI C'CwwG_G
Cuts at: 513 1065 2212 2948 513
Size: 552 1147 736 3074
Fragments arranged by size:
3074 1147 736 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3536 3553 3706 3891 4336 325
Size: 1255 1956 17 153 185 445 1498
Fragments arranged by size:
1956 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3755 5467 96
Size: 274 80 33 51 1443 1778 1712 138
Fragments arranged by size:
1778 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5469 5501 5469
Size: 32 5477
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5509
XbaI T'CTAG_A
Cuts at: 2845 2845
Size: 5509
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5509
XmaI C'CCGG_G
Cuts at: 1870 2616 2834 1870
Size: 746 218 4545
Fragments arranged by size:
4545 746 218
XmnI GAAnn'nnTTC
Cuts at: 3638 3638
Size: 5509
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3317 276
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI RsrII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI