Restriction map for
pFN21AB6360
(Circular) (Six-base) MAPSORT of: pFN21AB6360.seq Check: 6529 from: 1 to: 5596
pFN21AB6360 5596 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2447 2447
Size: 5596
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 1937
Size: 1002 4594
Acc65I G'GTAC_C
Cuts at: 2134 2917 2134
Size: 783 4813
AclI AA'CG_TT
Cuts at: 1563 2895 3723 4096 1563
Size: 1332 828 373 3063
Fragments arranged by size:
3063 1332 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2843 3659 5043 5311 1632
Size: 1211 816 1384 268 1917
Fragments arranged by size:
1917 1384 1211 816 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5399
AflIII A'CryG_T
Cuts at: 1203 4495 5472 1203
Size: 3292 977 1327
Fragments arranged by size:
3292 1327 977
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 4325 4325
Size: 5596
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2721 1184
Size: 1537 4059
AlwNI CAG_nnn'CTG
Cuts at: 2333 2821 3339 4911 2333
Size: 488 518 1572 3018
Fragments arranged by size:
3018 1572 518 488
ApaLI G'TGCA_C
Cuts at: 3653 4809 3653
Size: 1156 4440
ApoI r'AATT_y
Cuts at: 1493 2484 2671 2905 3131 3283 5392 1493
Size: 991 187 234 226 152 2109 1697
Fragments arranged by size:
2109 1697 991 234 226 187 152
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2921 1870
Size: 73 978 4545
Fragments arranged by size:
4545 978 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2126 2159 2126
Size: 33 5563
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2134 2540 2917 4372 618
Size: 325 217 627 347 406 377 1455 1842
Fragments arranged by size:
1842 1455 627 406 377 347 325 217
BanII G_rGCy'C
Cuts at: 1391 2915 1391
Size: 1524 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3787 3821 1903
Size: 34 1850 34 3678
Fragments arranged by size:
3678 1850 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3488 4698 3488
Size: 1210 4386
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4207 136
Size: 107 122 71 631 605 2535 1525
Fragments arranged by size:
2535 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5591 5591
Size: 5596
BlpI GC'TnA_GC
Cuts at: 3013 3013
Size: 5596
Bme1580I G_kGCm'C
Cuts at: 1790 3657 4813 1790
Size: 1867 1156 2573
Fragments arranged by size:
2573 1867 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5596
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2081 3327 4285
Size: 416 53 83 399 24 664 1246 958
Cuts at: 4285 442
Size: 1753
Fragments arranged by size:
1753 1246 958 664 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2629 2629
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4256 1239
Size: 3017 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3721 4586 4884 3721
Size: 865 298 4433
Fragments arranged by size:
4433 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4259 882
Size: 617 2760 2219
Fragments arranged by size:
2760 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3387 493
Size: 711 2183 2702
Fragments arranged by size:
2702 2183 711
BsaBI GATnn'nnATC
Cuts at: 5590 5590
Size: 5596
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3403 3785 4420 275
Size: 53 83 186 936 1870 382 635 1451
Fragments arranged by size:
1870 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4029 4427 4701 4848 5259 4029
Size: 398 274 147 411 4366
Fragments arranged by size:
4366 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5371
Fragments arranged by size:
5371 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 1186
Size: 844 4752
BseYI C'CCAG_C
Cuts at: 2352 2640 4799 5295 2352
Size: 288 2159 496 2653
Fragments arranged by size:
2653 2159 496 288
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2318 2475 1163
Size: 573 582 157 4284
Fragments arranged by size:
4284 582 573 157
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3046 3807 4835 1540
Size: 275 186 1045 761 1028 2301
Fragments arranged by size:
2301 1045 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3657 3742 4813 3657
Size: 85 1071 4440
Fragments arranged by size:
4440 1071 85
BsmI GAATG_Cn'
Cuts at: 2710 3107 3200 2710
Size: 397 93 5106
Fragments arranged by size:
5106 397 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2915 3657 3742 4813 1391
Size: 399 1125 742 85 1071 2174
Fragments arranged by size:
2174 1125 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5259 5259
Size: 5596
BspHI T'CATG_A
Cuts at: 3485 5215 3485
Size: 1730 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2447 2951 844
Size: 1603 504 3489
Fragments arranged by size:
3489 1603 504
BsrBI CCG'CTC
Cuts at: 1394 1823 3483 1394
Size: 429 1660 3507
Fragments arranged by size:
3507 1660 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4091 4265 65
Size: 1183 2843 174 1396
Fragments arranged by size:
2843 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4240 4427 1700
Size: 110 2430 187 2869
Fragments arranged by size:
2869 2430 187 110
BsrGI T'GTAC_A
Cuts at: 96 2474 96
Size: 2378 3218
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5596
BssSI C'ACGA_G
Cuts at: 3656 4668 3656
Size: 1012 4584
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5596
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5596
BstYI r'GATC_y
Cuts at: 1972 2317 2926 3293 3678 3695 5147 5233 5245
Size: 345 609 367 385 17 1452 86 12
Cuts at: 5245 5591 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 609 385 367 346 345 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5044
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4857
Fragments arranged by size:
4857 683 56
BtsI GCAGTG_nn'
Cuts at: 2065 3112 3906 3926 2065
Size: 1047 794 20 3735
Fragments arranged by size:
3735 1047 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5561
DraI TTT'AAA
Cuts at: 2900 2900
Size: 5596
DraIII CAC_nnn'GTG
Cuts at: 2758 2758
Size: 5596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4603 809
Size: 507 3287 1802
Fragments arranged by size:
3287 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3043 3932 5561
Size: 54 1425 50 275 18 1213 889 1629
Cuts at: 5561 8
Size: 43
Fragments arranged by size:
1629 1425 1213 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3043 1537
Size: 275 1231 4090
Fragments arranged by size:
4090 1231 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2007 2598 2736 3526 1353
Size: 654 591 138 790 3423
Fragments arranged by size:
3423 790 654 591 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2786 4183 4557 4703 387
Size: 1064 138 1197 1397 374 146 1280
Fragments arranged by size:
1397 1280 1197 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2843 3659 4256 5043 5311 1239
Size: 393 1211 816 597 787 268 1524
Fragments arranged by size:
1524 1211 816 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2671 2905 3283 2671
Size: 234 378 4984
Fragments arranged by size:
4984 378 234
FspI TGC'GCA
Cuts at: 4102 4102
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 4743 1678
Size: 3065 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2629 2661 4237 4269 4311
Size: 32 135 32 1057 32 1576 32 42
Cuts at: 4311 4343 1373
Size: 32 2626
Fragments arranged by size:
2626 1576 1057 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2748 2940 3186 669
Size: 988 281 810 192 246 3079
Fragments arranged by size:
3079 988 810 281 246 192
HindIII A'AGCT_T
Cuts at: 748 2234 748
Size: 1486 4110
HpaI GTT'AAC
Cuts at: 2748 3186 2748
Size: 438 5158
KpnI G_GTAC'C
Cuts at: 2138 2921 2138
Size: 783 4813
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 5472 5472
Size: 5596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4710 4894 1305
Size: 3405 184 2007
Fragments arranged by size:
3405 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3774
Fragments arranged by size:
3774 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3554 3913 4072 518
Size: 648 2388 359 159 2042
Fragments arranged by size:
2388 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3012 3689 4837 5082 1606
Size: 1406 677 1148 245 2120
Fragments arranged by size:
2120 1406 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5044
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 3043 3043
Size: 5596
NspI r_CATG'y
Cuts at: 1132 1229 2954 4499 1132
Size: 97 1725 1545 2229
Fragments arranged by size:
2229 1725 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5596
PciI A'CATG_T
Cuts at: 4495 4495
Size: 5596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5596
PfoI T'CCnGG_A
Cuts at: 2727 2727
Size: 5596
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 5178 5210 3665
Size: 32 1481 32 4051
Fragments arranged by size:
4051 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5596
PsiI TTA'TAA
Cuts at: 3166 3166
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2948 830
Size: 2118 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 2248 3844 5556 2248
Size: 1596 1712 2288
Fragments arranged by size:
2288 1712 1596
SfcI C'TryA_G
Cuts at: 826 1045 2717 2944 4079 4760 4951 826
Size: 219 1672 227 1135 681 191 1471
Fragments arranged by size:
1672 1471 1135 681 227 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5596
SmaI CCC'GGG
Cuts at: 1872 2923 1872
Size: 1051 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3700 4601 4863 820
Size: 197 926 1757 901 262 1553
Fragments arranged by size:
1757 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2954 1132
Size: 1822 3774
SspI AAT'ATT
Cuts at: 5 52 2125 2429 3520 5
Size: 47 2073 304 1091 2081
Fragments arranged by size:
2081 2073 1091 304 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5596
StyI C'CwwG_G
Cuts at: 513 1065 3035 513
Size: 552 1970 3074
Fragments arranged by size:
3074 1970 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3623 3640 3793 3978 4423 325
Size: 1255 2043 17 153 185 445 1498
Fragments arranged by size:
2043 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2246 2474 3842
Size: 274 80 33 51 1443 269 228 1368
Cuts at: 3842 5554 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1368 274 269 228 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5556 5588 5556
Size: 32 5564
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5596
XmaI C'CCGG_G
Cuts at: 1870 2921 1870
Size: 1051 4545
XmnI GAAnn'nnTTC
Cuts at: 2817 3725 2817
Size: 908 4688
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI