Restriction map for
pFN21AB6358
(Circular) (Six-base) MAPSORT of: pFN21AB6358.seq Check: 243 from: 1 to: 5593
pFN21AB6358 5593 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2707 2707
Size: 5593
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3403 278
Size: 53 83 186 936 1867 2468
Fragments arranged by size:
2468 1867 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2835 2936 1937
Size: 898 101 4594
Fragments arranged by size:
4594 898 101
Acc65I G'GTAC_C
Cuts at: 2914 2914
Size: 5593
AclI AA'CG_TT
Cuts at: 1563 3720 4093 1563
Size: 2157 373 3063
Fragments arranged by size:
3063 2157 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3656 5040 5308 1632
Size: 2024 1384 268 1917
Fragments arranged by size:
2024 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5396
AflIII A'CryG_T
Cuts at: 1203 4492 5469 1203
Size: 3289 977 1327
Fragments arranged by size:
3289 1327 977
AgeI A'CCGG_T
Cuts at: 4424 4424
Size: 5593
AhdI GACnn_n'nnGTC
Cuts at: 4322 4322
Size: 5593
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5593
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2390 1184
Size: 1206 4387
AlwNI CAG_nnn'CTG
Cuts at: 3336 4908 3336
Size: 1572 4021
ApaI G_GGCC'C
Cuts at: 2860 2860
Size: 5593
ApaLI G'TGCA_C
Cuts at: 3650 4806 3650
Size: 1156 4437
ApoI r'AATT_y
Cuts at: 1493 2902 3128 3280 5389 1493
Size: 1409 226 152 2109 1697
Fragments arranged by size:
2109 1697 1409 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4147 160
Size: 872 3115 1606
Fragments arranged by size:
3115 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5593
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 2156 2300 2626 2859 2918 1870
Size: 73 99 114 144 326 233 59 4545
Fragments arranged by size:
4545 326 233 144 114 99 73 59
BamHI G'GATC_C
Cuts at: 2923 3290 2923
Size: 367 5226
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2255 2296 2361 2914 4369
Size: 325 217 627 468 41 65 553 1455
Cuts at: 4369 618
Size: 1842
Fragments arranged by size:
1842 1455 627 553 468 325 217 65
41
BanII G_rGCy'C
Cuts at: 1391 2661 2860 2912 1391
Size: 1270 199 52 4072
Fragments arranged by size:
4072 1270 199 52
BbeI G_GCGC'C
Cuts at: 2259 2365 2259
Size: 106 5487
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4622
BbvCI CC'TCA_GC
Cuts at: 2085 2562 2085
Size: 477 5116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3784 3818 1903
Size: 34 1847 34 3678
Fragments arranged by size:
3678 1847 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3485 4695 3485
Size: 1210 4383
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5593
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4204 136
Size: 107 122 71 631 605 2532 1525
Fragments arranged by size:
2532 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2760 5588 2760
Size: 2828 2765
BlpI GC'TnA_GC
Cuts at: 3010 3010
Size: 5593
Bme1580I G_kGCm'C
Cuts at: 1790 2301 2730 2860 3654 4810 1790
Size: 511 429 130 794 1156 2573
Fragments arranged by size:
2573 1156 794 511 429 130
BmgBI CAC'GTC
Cuts at: 1318 2419 1318
Size: 1101 4492
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2581 3324 4282
Size: 416 53 83 399 24 1164 743 958
Cuts at: 4282 442
Size: 1753
Fragments arranged by size:
1753 1164 958 743 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2461 2481 4253 1239
Size: 1222 20 1772 2579
Fragments arranged by size:
2579 1772 1222 20
Bpu10I CC'TnA_GC
Cuts at: 2085 2351 2562 2085
Size: 266 211 5116
Fragments arranged by size:
5116 266 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2258 3718 4583 4881 2258
Size: 1460 865 298 2970
Fragments arranged by size:
2970 1460 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4256 882
Size: 617 2757 2219
Fragments arranged by size:
2757 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3384 493
Size: 711 2180 2702
Fragments arranged by size:
2702 2180 711
BsaBI GATnn'nnATC
Cuts at: 2759 5587 2759
Size: 2828 2765
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2256 2362 2793 3400
Size: 53 83 186 936 723 106 431 607
Cuts at: 3400 3782 4417 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 723 635 607 431 382 186
106 83 53
BsaWI w'CCGG_w
Cuts at: 4026 4424 4698 4845 5256 4026
Size: 398 274 147 411 4363
Fragments arranged by size:
4363 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2498 2528 1178
Size: 30 165 30 1095 30 4243
Fragments arranged by size:
4243 1095 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2075 2087 2102 2105 2129 2132 2330
Size: 874 15 12 15 3 24 3 198
Cuts at: 2330 2333 2465 2506 2591 1186
Size: 3 132 41 85 4188
Fragments arranged by size:
4188 874 198 132 85 41 24 15
15 12 3 3 3
BseYI C'CCAG_C
Cuts at: 4796 5292 4796
Size: 496 5097
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2228 1163
Size: 573 492 4528
Fragments arranged by size:
4528 573 492
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2099 2130 2196 3043 3804 4832
Size: 275 186 98 31 66 847 761 1028
Cuts at: 4832 1540
Size: 2301
Fragments arranged by size:
2301 1028 847 761 275 186 98 66
31
BsiHKAI G_wGCw'C
Cuts at: 2661 2685 2817 3654 3739 4810 2661
Size: 24 132 837 85 1071 3444
Fragments arranged by size:
3444 1071 837 132 85 24
BsmI GAATG_Cn'
Cuts at: 3104 3197 3104
Size: 93 5500
BsmBI CGTCTCn'nnnn_
Cuts at: 2007 2007
Size: 5593
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2301 2661 2685 2730 2817 2860 2912
Size: 399 511 360 24 45 87 43 52
Cuts at: 2912 3654 3739 4810 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 511 399 360 87 85
52 45 43 24
BspEI T'CCGG_A
Cuts at: 5256 5256
Size: 5593
BspHI T'CATG_A
Cuts at: 3482 5212 3482
Size: 1730 3863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2562 2707 2948 844
Size: 1718 145 241 3489
Fragments arranged by size:
3489 1718 241 145
BsrBI CCG'CTC
Cuts at: 1394 1823 2095 3480 1394
Size: 429 272 1385 3507
Fragments arranged by size:
3507 1385 429 272
BsrDI GCAATG_nn'
Cuts at: 65 1248 4088 4262 65
Size: 1183 2840 174 1396
Fragments arranged by size:
2840 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2191 2599 4237 4424 1700
Size: 110 381 408 1638 187 2869
Fragments arranged by size:
2869 1638 408 381 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5593
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5593
BssSI C'ACGA_G
Cuts at: 2684 3653 4665 2684
Size: 969 1012 3612
Fragments arranged by size:
3612 1012 969
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5593
BstBI TT'CG_AA
Cuts at: 4397 4397
Size: 5593
BstEII G'GTnAC_C
Cuts at: 1184 2018 2863 1184
Size: 834 845 3914
Fragments arranged by size:
3914 845 834
BstYI r'GATC_y
Cuts at: 1972 2760 2923 3290 3675 3692 5144 5230 5242
Size: 788 163 367 385 17 1452 86 12
Cuts at: 5242 5588 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 788 385 367 346 163 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4102
Fragments arranged by size:
4102 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4854
Fragments arranged by size:
4854 683 56
BtsI GCAGTG_nn'
Cuts at: 2186 2730 3109 3903 3923 2186
Size: 544 379 794 20 3856
Fragments arranged by size:
3856 794 544 379 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5593
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5558
DraI TTT'AAA
Cuts at: 2897 2897
Size: 5593
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4600 809
Size: 507 3284 1802
Fragments arranged by size:
3284 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2096 2127 2181
Size: 54 1425 50 275 18 266 31 54
Cuts at: 2181 2193 2601 3040 3929 5558 8
Size: 12 408 439 889 1629 43
Fragments arranged by size:
1629 1425 889 439 408 275 266 54
54 50 43 31 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2096 2127 2193 3040 1537
Size: 275 284 31 66 847 4090
Fragments arranged by size:
4090 847 284 275 66 31
EarI CTCTTCn'nnn_
Cuts at: 1353 2388 3523 1353
Size: 1035 1135 3423
Fragments arranged by size:
3423 1135 1035
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4180 4554 4700 387
Size: 1064 138 2591 374 146 1280
Fragments arranged by size:
2591 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2659 2659
Size: 5593
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2461 2481 3656 4253 5040 5308 1239
Size: 393 829 20 1175 597 787 268 1524
Fragments arranged by size:
1524 1175 829 787 597 393 268 20
EcoO109I rG'GnC_Cy
Cuts at: 2856 2856
Size: 5593
EcoRI G'AATT_C
Cuts at: 2902 3280 2902
Size: 378 5215
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 5593
FspI TGC'GCA
Cuts at: 4099 4099
Size: 5593
HaeII r_GCGC'y
Cuts at: 1678 2259 2365 4740 1678
Size: 581 106 2375 2531
Fragments arranged by size:
2531 2375 581 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2609 2641 4234 4266 4308
Size: 32 135 32 1037 32 1593 32 42
Cuts at: 4308 4340 1373
Size: 32 2626
Fragments arranged by size:
2626 1593 1037 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2937 3183 669
Size: 988 281 999 246 3079
Fragments arranged by size:
3079 999 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5593
HpaI GTT'AAC
Cuts at: 3183 3183
Size: 5593
KasI G'GCGC_C
Cuts at: 2255 2361 2255
Size: 106 5487
KpnI G_GTAC'C
Cuts at: 2918 2918
Size: 5593
MfeI C'AATT_G
Cuts at: 3192 3192
Size: 5593
MluI A'CGCG_T
Cuts at: 5469 5469
Size: 5593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4707 4891 1305
Size: 3402 184 2007
Fragments arranged by size:
3402 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3771
Fragments arranged by size:
3771 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2609 3551 3910 4069 518
Size: 648 1443 942 359 159 2042
Fragments arranged by size:
2042 1443 942 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2164 2263 2712 3009 3686 4834 5079 1606
Size: 558 99 449 297 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 558 449 297 245 99
NaeI GCC'GGC
Cuts at: 2193 2601 2193
Size: 408 5185
NarI GG'CG_CC
Cuts at: 2256 2362 2256
Size: 106 5487
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4102
Fragments arranged by size:
4102 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5593
NgoMIV G'CCGG_C
Cuts at: 2191 2599 2191
Size: 408 5185
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5593
NotI GC'GGCC_GC
Cuts at: 2127 3040 2127
Size: 913 4680
NspI r_CATG'y
Cuts at: 1132 1229 2951 4496 1132
Size: 97 1722 1545 2229
Fragments arranged by size:
2229 1722 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5593
PciI A'CATG_T
Cuts at: 4492 4492
Size: 5593
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5593
PmeI GTTT'AAAC
Cuts at: 2897 2897
Size: 5593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3662 3694 5175 5207 3662
Size: 32 1481 32 4048
Fragments arranged by size:
4048 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5593
PsiI TTA'TAA
Cuts at: 3163 3163
Size: 5593
PspOMI G'GGCC_C
Cuts at: 2856 2856
Size: 5593
PstI C_TGCA'G
Cuts at: 830 2559 2945 830
Size: 1729 386 3478
Fragments arranged by size:
3478 1729 386
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5593
SacI G_AGCT'C
Cuts at: 2661 2661
Size: 5593
SalI G'TCGA_C
Cuts at: 1936 2935 1936
Size: 999 4594
SbfI CC_TGCA'GG
Cuts at: 2559 2945 2559
Size: 386 5207
ScaI AGT'ACT
Cuts at: 2507 3841 5553 2507
Size: 1334 1712 2547
Fragments arranged by size:
2547 1712 1334
SfcI C'TryA_G
Cuts at: 826 1045 2555 2941 4076 4757 4948 826
Size: 219 1510 386 1135 681 191 1471
Fragments arranged by size:
1510 1471 1135 681 386 219 191
SfoI GGC'GCC
Cuts at: 2257 2363 2257
Size: 106 5487
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5593
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5593
SmaI CCC'GGG
Cuts at: 1872 2861 2920 1872
Size: 989 59 4545
Fragments arranged by size:
4545 989 59
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2273 3697 4598 4860 820
Size: 197 926 330 1424 901 262 1553
Fragments arranged by size:
1553 1424 926 901 330 262 197
SnaBI TAC'GTA
Cuts at: 493 3384 493
Size: 2891 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5593
SphI G_CATG'C
Cuts at: 1132 2951 1132
Size: 1819 3774
SspI AAT'ATT
Cuts at: 5 52 3517 5
Size: 47 3465 2081
Fragments arranged by size:
3465 2081 47
StuI AGG'CCT
Cuts at: 1517 2213 1517
Size: 696 4897
StyI C'CwwG_G
Cuts at: 513 1065 2004 3032 513
Size: 552 939 1028 3074
Fragments arranged by size:
3074 1028 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2498 3620 3637 3790 3975 4420 325
Size: 1255 918 1122 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1122 918 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2505 3839 5551
Size: 274 80 33 51 1443 528 1334 1712
Cuts at: 5551 96
Size: 138
Fragments arranged by size:
1712 1443 1334 528 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2396 2428 5553 5585 2396
Size: 32 3125 32 2404
Fragments arranged by size:
3125 2404 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2831 1316
Size: 1515 4078
XbaI T'CTAG_A
Cuts at: 2929 2929
Size: 5593
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5593
XmaI C'CCGG_G
Cuts at: 1870 2859 2918 1870
Size: 989 59 4545
Fragments arranged by size:
4545 989 59
XmnI GAAnn'nnTTC
Cuts at: 2507 3722 2507
Size: 1215 4378
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3401 276
Size: 53 83 186 936 1867 2468
Fragments arranged by size:
2468 1867 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI