Restriction map for
pFN21AB6354
(Circular) (Six-base) MAPSORT of: pFN21AB6354.seq Check: 8728 from: 1 to: 5590
pFN21AB6354 5590 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2353 2353
Size: 5590
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3400 278
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2933 1937
Size: 996 4594
Acc65I G'GTAC_C
Cuts at: 2911 2911
Size: 5590
AclI AA'CG_TT
Cuts at: 1563 3717 4090 1563
Size: 2154 373 3063
Fragments arranged by size:
3063 2154 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2720 3653 5037 5305 1632
Size: 1088 933 1384 268 1917
Fragments arranged by size:
1917 1384 1088 933 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5393
AflIII A'CryG_T
Cuts at: 1203 4489 5466 1203
Size: 3286 977 1327
Fragments arranged by size:
3286 1327 977
AgeI A'CCGG_T
Cuts at: 4421 4421
Size: 5590
AhdI GACnn_n'nnGTC
Cuts at: 4319 4319
Size: 5590
AleI CACnn'nnGTG
Cuts at: 1166 2819 1166
Size: 1653 3937
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5590
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2669 2701 2669
Size: 32 5558
AlwNI CAG_nnn'CTG
Cuts at: 3333 4905 3333
Size: 1572 4018
ApaI G_GGCC'C
Cuts at: 2018 2018
Size: 5590
ApaLI G'TGCA_C
Cuts at: 2737 3647 4803 2737
Size: 910 1156 3524
Fragments arranged by size:
3524 1156 910
ApoI r'AATT_y
Cuts at: 1493 2899 3125 3277 5386 1493
Size: 1406 226 152 2109 1697
Fragments arranged by size:
2109 1697 1406 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4144 160
Size: 872 3112 1606
Fragments arranged by size:
3112 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
AvaI C'yCGr_G
Cuts at: 1870 1943 2915 1870
Size: 73 972 4545
Fragments arranged by size:
4545 972 73
BamHI G'GATC_C
Cuts at: 2241 2920 3287 2241
Size: 679 367 4544
Fragments arranged by size:
4544 679 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2911 4366 618
Size: 325 217 627 1124 1455 1842
Fragments arranged by size:
1842 1455 1124 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2018 2066 2079 2909 1391
Size: 627 48 13 830 4072
Fragments arranged by size:
4072 830 627 48 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4619
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2449 2483 3781 3815 1903
Size: 34 50 34 428 34 1298 34 3678
Fragments arranged by size:
3678 1298 428 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3482 4692 3482
Size: 1210 4380
BclI T'GATC_A
Cuts at: 1548 2811 1548
Size: 1263 4327
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4201 136
Size: 107 122 71 631 605 2529 1525
Fragments arranged by size:
2529 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5585 5585
Size: 5590
BlpI GC'TnA_GC
Cuts at: 3007 3007
Size: 5590
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2741 3651 4807 1790
Size: 228 723 910 1156 2573
Fragments arranged by size:
2573 1156 910 723 228
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5590
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3321 4279 442
Size: 416 53 83 399 24 1904 958 1753
Fragments arranged by size:
1904 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2475 1056
Size: 1419 4171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2243 2666 4250 1239
Size: 1004 423 1584 2579
Fragments arranged by size:
2579 1584 1004 423
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2444 3715 4580 4878 2444
Size: 1271 865 298 3156
Fragments arranged by size:
3156 1271 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2198 2324 2642 4253 882
Size: 617 699 126 318 1611 2219
Fragments arranged by size:
2219 1611 699 617 318 126
BsaAI yAC'GTr
Cuts at: 493 1204 3381 493
Size: 711 2177 2702
Fragments arranged by size:
2702 2177 711
BsaBI GATnn'nnATC
Cuts at: 5584 5584
Size: 5590
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3397 3779 4414 275
Size: 53 83 186 936 1864 382 635 1451
Fragments arranged by size:
1864 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4023 4421 4695 4842 5253 4023
Size: 398 274 147 411 4360
Fragments arranged by size:
4360 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5365
Fragments arranged by size:
5365 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2762 1186
Size: 1576 4014
BseYI C'CCAG_C
Cuts at: 2194 4793 5289 2194
Size: 2599 496 2495
Fragments arranged by size:
2599 2495 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 1163
Size: 573 645 4372
Fragments arranged by size:
4372 645 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2434 3040 3801 4829 1540
Size: 275 186 433 606 761 1028 2301
Fragments arranged by size:
2301 1028 761 606 433 275 186
BsiHKAI G_wGCw'C
Cuts at: 2294 2741 3651 3736 4807 2294
Size: 447 910 85 1071 3077
Fragments arranged by size:
3077 1071 910 447 85
BsmI GAATG_Cn'
Cuts at: 3101 3194 3101
Size: 93 5497
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2066 2079 2294 2741 2909 3651
Size: 399 228 48 13 215 447 168 742
Cuts at: 3651 3736 4807 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 447 399 228 215 168
85 48 13
BspEI T'CCGG_A
Cuts at: 5253 5253
Size: 5590
BspHI T'CATG_A
Cuts at: 2253 3479 5209 2253
Size: 1226 1730 2634
Fragments arranged by size:
2634 1730 1226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2151 2199 2353 2945 844
Size: 1307 48 154 592 3489
Fragments arranged by size:
3489 1307 592 154 48
BsrBI CCG'CTC
Cuts at: 1394 1823 3477 1394
Size: 429 1654 3507
Fragments arranged by size:
3507 1654 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4085 4259 65
Size: 1183 2837 174 1396
Fragments arranged by size:
2837 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2231 4234 4421 1700
Size: 110 421 2003 187 2869
Fragments arranged by size:
2869 2003 421 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5590
BssHII G'CGCG_C
Cuts at: 1925 2069 1925
Size: 144 5446
BssSI C'ACGA_G
Cuts at: 2201 3650 4662 2201
Size: 1449 1012 3129
Fragments arranged by size:
3129 1449 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2121 794
Size: 1327 4263
BstBI TT'CG_AA
Cuts at: 4394 4394
Size: 5590
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5590
BstXI CCAn_nnnn'nTGG
Cuts at: 2194 2194
Size: 5590
BstYI r'GATC_y
Cuts at: 1972 2241 2920 3287 3672 3689 5141 5227 5239
Size: 269 679 367 385 17 1452 86 12
Cuts at: 5239 5585 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 679 385 367 346 269 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2155 513
Size: 552 1090 3948
Fragments arranged by size:
3948 1090 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4851
Fragments arranged by size:
4851 683 56
BtsI GCAGTG_nn'
Cuts at: 2050 2267 2741 2891 3106 3900 3920 2050
Size: 217 474 150 215 794 20 3720
Fragments arranged by size:
3720 794 474 217 215 150 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5590
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5555
DraI TTT'AAA
Cuts at: 2894 2894
Size: 5590
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4597 809
Size: 507 3281 1802
Fragments arranged by size:
3281 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 2136 3037
Size: 54 1425 50 275 18 259 47 901
Cuts at: 3037 3926 5555 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 901 889 275 259 54 50
47 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3037 1537
Size: 275 1225 4090
Fragments arranged by size:
4090 1225 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 2084 2303 3520 1353
Size: 689 42 219 1217 3423
Fragments arranged by size:
3423 1217 689 219 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2383 4177 4551 4697 387
Size: 1064 138 794 1794 374 146 1280
Fragments arranged by size:
1794 1280 1064 794 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2666 2720 3653 4250 5037 5305
Size: 393 611 423 54 933 597 787 268
Cuts at: 5305 1239
Size: 1524
Fragments arranged by size:
1524 933 787 611 597 423 393 268
54
EcoO109I rG'GnC_Cy
Cuts at: 2014 2015 2695 2014
Size: 1 680 4909
Fragments arranged by size:
4909 680 1
EcoRI G'AATT_C
Cuts at: 2899 3277 2899
Size: 378 5212
FspI TGC'GCA
Cuts at: 4096 4096
Size: 5590
HaeII r_GCGC'y
Cuts at: 1678 2238 4737 1678
Size: 560 2499 2531
Fragments arranged by size:
2531 2499 560
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4231 4263 4305 4337 1373
Size: 32 135 32 2659 32 42 32 2626
Fragments arranged by size:
2659 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2934 3180 669
Size: 988 281 996 246 3079
Fragments arranged by size:
3079 996 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5590
HpaI GTT'AAC
Cuts at: 3180 3180
Size: 5590
KpnI G_GTAC'C
Cuts at: 2915 2915
Size: 5590
MfeI C'AATT_G
Cuts at: 2119 3189 2119
Size: 1070 4520
MluI A'CGCG_T
Cuts at: 5466 5466
Size: 5590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4704 4888 1305
Size: 3399 184 2007
Fragments arranged by size:
3399 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2091 10
Size: 54 1425 343 259 3509
Fragments arranged by size:
3509 1425 343 259 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2819 3548 3907 4066 518
Size: 648 1653 729 359 159 2042
Fragments arranged by size:
2042 1653 729 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2149 2248 2376 3006 3683 4831 5076 1606
Size: 543 99 128 630 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 630 543 245 128 99
NaeI GCC'GGC
Cuts at: 2233 2233
Size: 5590
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5038
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5590
NgoMIV G'CCGG_C
Cuts at: 2231 2231
Size: 5590
NheI G'CTAG_C
Cuts at: 1052 2471 1052
Size: 1419 4171
NotI GC'GGCC_GC
Cuts at: 3037 3037
Size: 5590
NspI r_CATG'y
Cuts at: 1132 1229 2179 2422 2948 4493 1132
Size: 97 950 243 526 1545 2229
Fragments arranged by size:
2229 1545 950 526 243 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5590
PciI A'CATG_T
Cuts at: 4489 4489
Size: 5590
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5590
PfoI T'CCnGG_A
Cuts at: 2643 2643
Size: 5590
PmeI GTTT'AAAC
Cuts at: 2894 2894
Size: 5590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 5172 5204 3659
Size: 32 1481 32 4045
Fragments arranged by size:
4045 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2695 2695
Size: 5590
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5590
PsiI TTA'TAA
Cuts at: 3160 3160
Size: 5590
PspOMI G'GGCC_C
Cuts at: 2014 2014
Size: 5590
PstI C_TGCA'G
Cuts at: 830 2013 2148 2886 2942 830
Size: 1183 135 738 56 3478
Fragments arranged by size:
3478 1183 738 135 56
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5590
PvuII CAG'CTG
Cuts at: 2149 2149
Size: 5590
SalI G'TCGA_C
Cuts at: 1936 2932 1936
Size: 996 4594
SapI GCTCTTCn'nnn_
Cuts at: 2042 2084 2303 2042
Size: 42 219 5329
Fragments arranged by size:
5329 219 42
SbfI CC_TGCA'GG
Cuts at: 2942 2942
Size: 5590
ScaI AGT'ACT
Cuts at: 3838 5550 3838
Size: 1712 3878
SfcI C'TryA_G
Cuts at: 826 1045 2009 2144 2882 2938 4073 4754 4945
Size: 219 964 135 738 56 1135 681 191
Cuts at: 4945 826
Size: 1471
Fragments arranged by size:
1471 1135 964 738 681 219 191 135
56
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5590
SmaI CCC'GGG
Cuts at: 1872 2917 1872
Size: 1045 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2423 3694 4595 4857 820
Size: 197 926 480 1271 901 262 1553
Fragments arranged by size:
1553 1271 926 901 480 262 197
SnaBI TAC'GTA
Cuts at: 493 3381 493
Size: 2888 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5590
SphI G_CATG'C
Cuts at: 1132 2948 1132
Size: 1816 3774
SspI AAT'ATT
Cuts at: 5 52 3514 5
Size: 47 3462 2081
Fragments arranged by size:
3462 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5590
StyI C'CwwG_G
Cuts at: 513 1065 3029 513
Size: 552 1964 3074
Fragments arranged by size:
3074 1964 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3617 3634 3787 3972 4417 325
Size: 1255 2037 17 153 185 445 1498
Fragments arranged by size:
2037 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3836 5548 96
Size: 274 80 33 51 1443 1859 1712 138
Fragments arranged by size:
1859 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5550 5582 5550
Size: 32 5558
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5590
XbaI T'CTAG_A
Cuts at: 2926 2926
Size: 5590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2641 2641
Size: 5590
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5590
XmaI C'CCGG_G
Cuts at: 1870 2915 1870
Size: 1045 4545
XmnI GAAnn'nnTTC
Cuts at: 3719 3719
Size: 5590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3398 276
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI