Restriction map for
pFN21AB6346
(Circular) (Six-base) MAPSORT of: pFN21AB6346.seq Check: 1951 from: 1 to: 5578
pFN21AB6346 5578 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2808 3388 278
Size: 53 83 186 936 1272 580 2468
Fragments arranged by size:
2468 1272 936 580 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2921 1937
Size: 984 4594
Acc65I G'GTAC_C
Cuts at: 2899 2899
Size: 5578
AclI AA'CG_TT
Cuts at: 1563 3705 4078 1563
Size: 2142 373 3063
Fragments arranged by size:
3063 2142 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2012 3641 5025 5293 1632
Size: 380 1629 1384 268 1917
Fragments arranged by size:
1917 1629 1384 380 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5381
AflIII A'CryG_T
Cuts at: 1203 4477 5454 1203
Size: 3274 977 1327
Fragments arranged by size:
3274 1327 977
AgeI A'CCGG_T
Cuts at: 4409 4409
Size: 5578
AhdI GACnn_n'nnGTC
Cuts at: 4307 4307
Size: 5578
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5578
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5578
AlwNI CAG_nnn'CTG
Cuts at: 2035 3321 4893 2035
Size: 1286 1572 2720
Fragments arranged by size:
2720 1572 1286
ApaI G_GGCC'C
Cuts at: 2664 2664
Size: 5578
ApaLI G'TGCA_C
Cuts at: 3635 4791 3635
Size: 1156 4422
ApoI r'AATT_y
Cuts at: 1493 2600 2887 3113 3265 5374 1493
Size: 1107 287 226 152 2109 1697
Fragments arranged by size:
2109 1697 1107 287 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4132 160
Size: 872 3100 1606
Fragments arranged by size:
3100 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
AvaI C'yCGr_G
Cuts at: 1870 1943 2903 1870
Size: 73 960 4545
Fragments arranged by size:
4545 960 73
BamHI G'GATC_C
Cuts at: 2183 2908 3275 2183
Size: 725 367 4486
Fragments arranged by size:
4486 725 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2899 4354 618
Size: 325 217 627 1112 1455 1842
Fragments arranged by size:
1842 1455 1112 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2664 2751 2897 1391
Size: 1273 87 146 4072
Fragments arranged by size:
4072 1273 146 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4607
BbvCI CC'TCA_GC
Cuts at: 2147 2147
Size: 5578
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2750 2784 3769 3803 1903
Size: 34 813 34 985 34 3678
Fragments arranged by size:
3678 985 813 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3470 4680 3470
Size: 1210 4368
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5578
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4189 136
Size: 107 122 71 631 605 2517 1525
Fragments arranged by size:
2517 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5573 5573
Size: 5578
BlpI GC'TnA_GC
Cuts at: 2995 2995
Size: 5578
Bme1580I G_kGCm'C
Cuts at: 1790 2664 2773 3639 4795 1790
Size: 874 109 866 1156 2573
Fragments arranged by size:
2573 1156 874 866 109
BmgBI CAC'GTC
Cuts at: 1318 2583 1318
Size: 1265 4313
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2485 3309 4267
Size: 416 53 83 399 24 1068 824 958
Cuts at: 4267 442
Size: 1753
Fragments arranged by size:
1753 1068 958 824 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2350 2351 2420 2576 2731 4238 1239
Size: 1111 1 69 156 155 1507 2579
Fragments arranged by size:
2579 1507 1111 156 155 69 1
Bpu10I CC'TnA_GC
Cuts at: 2147 2414 2147
Size: 267 5311
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2296 3703 4568 4866 2296
Size: 1407 865 298 3008
Fragments arranged by size:
3008 1407 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4241 882
Size: 617 2742 2219
Fragments arranged by size:
2742 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2383 3369 493
Size: 711 1179 986 2702
Fragments arranged by size:
2702 1179 986 711
BsaBI GATnn'nnATC
Cuts at: 5572 5572
Size: 5578
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2535 2805 3385 3767
Size: 53 83 186 936 1002 270 580 382
Cuts at: 3767 4402 275
Size: 635 1451
Fragments arranged by size:
1451 1002 936 635 580 382 270 186
83 53
BsaWI w'CCGG_w
Cuts at: 2114 4011 4409 4683 4830 5241 2114
Size: 1897 398 274 147 411 2451
Fragments arranged by size:
2451 1897 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2109 2139 2280 2310 1178
Size: 30 165 30 706 30 141 30 4446
Fragments arranged by size:
4446 706 165 141 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2073 2117 2222 2606 2769 1186
Size: 874 13 44 105 384 163 3995
Fragments arranged by size:
3995 874 384 163 105 44 13
BseYI C'CCAG_C
Cuts at: 2088 2196 2325 2826 4781 5277 2088
Size: 108 129 501 1955 496 2389
Fragments arranged by size:
2389 1955 501 496 129 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5005
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2791 3028 3789 4817 1540
Size: 275 186 790 237 761 1028 2301
Fragments arranged by size:
2301 1028 790 761 275 237 186
BsiHKAI G_wGCw'C
Cuts at: 2255 2282 2487 3639 3724 4795 2255
Size: 27 205 1152 85 1071 3038
Fragments arranged by size:
3038 1152 1071 205 85 27
BsmI GAATG_Cn'
Cuts at: 3089 3182 3089
Size: 93 5485
BsmBI CGTCTCn'nnnn_
Cuts at: 2111 2222 2687 2111
Size: 111 465 5002
Fragments arranged by size:
5002 465 111
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2255 2282 2487 2664 2751 2773 2897
Size: 399 465 27 205 177 87 22 124
Cuts at: 2897 3639 3724 4795 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 465 399 205 177 124
87 85 27 22
BspEI T'CCGG_A
Cuts at: 2114 5241 2114
Size: 3127 2451
BspHI T'CATG_A
Cuts at: 3467 5197 3467
Size: 1730 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2412 2468 2933 844
Size: 1568 56 465 3489
Fragments arranged by size:
3489 1568 465 56
BsrBI CCG'CTC
Cuts at: 1394 1823 3465 1394
Size: 429 1642 3507
Fragments arranged by size:
3507 1642 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2299 2449 4073 4247 65
Size: 1183 1051 150 1624 174 1396
Fragments arranged by size:
1624 1396 1183 1051 174 150
BsrFI r'CCGG_y
Cuts at: 1700 1810 2550 4222 4409 1700
Size: 110 740 1672 187 2869
Fragments arranged by size:
2869 1672 740 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5578
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5578
BssSI C'ACGA_G
Cuts at: 2486 3638 4650 2486
Size: 1152 1012 3414
Fragments arranged by size:
3414 1152 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5578
BstBI TT'CG_AA
Cuts at: 4382 4382
Size: 5578
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5578
BstXI CCAn_nnnn'nTGG
Cuts at: 2444 2444
Size: 5578
BstYI r'GATC_y
Cuts at: 1972 2183 2908 3275 3660 3677 5129 5215 5227
Size: 211 725 367 385 17 1452 86 12
Cuts at: 5227 5573 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 725 385 367 346 211 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2743 513
Size: 552 1678 3348
Fragments arranged by size:
3348 1678 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4839
Fragments arranged by size:
4839 683 56
BtsI GCAGTG_nn'
Cuts at: 3094 3888 3908 3094
Size: 794 20 4764
Fragments arranged by size:
4764 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5578
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5543
DraI TTT'AAA
Cuts at: 2882 2882
Size: 5578
DraIII CAC_nnn'GTG
Cuts at: 2572 2572
Size: 5578
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4585 809
Size: 507 3269 1802
Fragments arranged by size:
3269 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2740 2788 3025
Size: 54 1425 50 275 18 910 48 237
Cuts at: 3025 3914 5543 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 910 889 275 237 54 50
48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2788 3025 1537
Size: 275 976 237 4090
Fragments arranged by size:
4090 976 275 237
EarI CTCTTCn'nnn_
Cuts at: 1353 3508 1353
Size: 2155 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4165 4539 4685 387
Size: 1064 138 2576 374 146 1280
Fragments arranged by size:
2576 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2012 2350 2351 2420 2576 2731 3641
Size: 393 380 338 1 69 156 155 910
Cuts at: 3641 4238 5025 5293 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 910 787 597 393 380 338 268
156 155 69 1
EcoO109I rG'GnC_Cy
Cuts at: 2218 2347 2457 2621 2661 2817 2218
Size: 129 110 164 40 156 4979
Fragments arranged by size:
4979 164 156 129 110 40
EcoRI G'AATT_C
Cuts at: 2887 3265 2887
Size: 378 5200
FspI TGC'GCA
Cuts at: 4084 4084
Size: 5578
HaeII r_GCGC'y
Cuts at: 1678 2435 4725 1678
Size: 757 2290 2531
Fragments arranged by size:
2531 2290 757
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2109 2141 2344 2376 4219
Size: 32 135 32 537 32 203 32 1843
Cuts at: 4219 4251 4293 4325 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1843 537 203 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2473 2922 3168 669
Size: 988 281 535 449 246 3079
Fragments arranged by size:
3079 988 535 449 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5578
HpaI GTT'AAC
Cuts at: 3168 3168
Size: 5578
KpnI G_GTAC'C
Cuts at: 2903 2903
Size: 5578
MfeI C'AATT_G
Cuts at: 3177 3177
Size: 5578
MluI A'CGCG_T
Cuts at: 5454 5454
Size: 5578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4692 4876 1305
Size: 3387 184 2007
Fragments arranged by size:
3387 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3756
Fragments arranged by size:
3756 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2636 3536 3895 4054 518
Size: 648 1470 900 359 159 2042
Fragments arranged by size:
2042 1470 900 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2200 2329 2496 2793 2826 2994 3671 4819
Size: 594 129 167 297 33 168 677 1148
Cuts at: 4819 5064 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 594 297 245 168 167
129 33
NaeI GCC'GGC
Cuts at: 2552 2552
Size: 5578
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5026
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5578
NgoMIV G'CCGG_C
Cuts at: 2550 2550
Size: 5578
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5578
NotI GC'GGCC_GC
Cuts at: 3025 3025
Size: 5578
NspI r_CATG'y
Cuts at: 1132 1229 2936 4481 1132
Size: 97 1707 1545 2229
Fragments arranged by size:
2229 1707 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2140 2284 1348
Size: 792 144 4642
Fragments arranged by size:
4642 792 144
PciI A'CATG_T
Cuts at: 4477 4477
Size: 5578
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5578
PfoI T'CCnGG_A
Cuts at: 2516 2516
Size: 5578
PmeI GTTT'AAAC
Cuts at: 2882 2882
Size: 5578
PmlI CAC'GTG
Cuts at: 2383 2383
Size: 5578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 5160 5192 3647
Size: 32 1481 32 4033
Fragments arranged by size:
4033 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2347 2457 2347
Size: 110 5468
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5578
PsiI TTA'TAA
Cuts at: 3148 3148
Size: 5578
PspOMI G'GGCC_C
Cuts at: 2660 2660
Size: 5578
PstI C_TGCA'G
Cuts at: 830 2103 2205 2930 830
Size: 1273 102 725 3478
Fragments arranged by size:
3478 1273 725 102
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5578
PvuII CAG'CTG
Cuts at: 2200 2329 2496 2200
Size: 129 167 5282
Fragments arranged by size:
5282 167 129
SalI G'TCGA_C
Cuts at: 1936 2920 1936
Size: 984 4594
SbfI CC_TGCA'GG
Cuts at: 2930 2930
Size: 5578
ScaI AGT'ACT
Cuts at: 3826 5538 3826
Size: 1712 3866
SexAI A'CCwGG_T
Cuts at: 2568 2568
Size: 5578
SfcI C'TryA_G
Cuts at: 826 1045 2099 2201 2926 4061 4742 4933 826
Size: 219 1054 102 725 1135 681 191 1471
Fragments arranged by size:
1471 1135 1054 725 681 219 191 102
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5578
SmaI CCC'GGG
Cuts at: 1872 2905 1872
Size: 1033 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2275 3682 4583 4845 820
Size: 197 926 332 1407 901 262 1553
Fragments arranged by size:
1553 1407 926 901 332 262 197
SnaBI TAC'GTA
Cuts at: 493 3369 493
Size: 2876 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5578
SphI G_CATG'C
Cuts at: 1132 2936 1132
Size: 1804 3774
SspI AAT'ATT
Cuts at: 5 52 3502 5
Size: 47 3450 2081
Fragments arranged by size:
3450 2081 47
StuI AGG'CCT
Cuts at: 1517 2525 1517
Size: 1008 4570
StyI C'CwwG_G
Cuts at: 513 1065 2010 3017 513
Size: 552 945 1007 3074
Fragments arranged by size:
3074 1007 945 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2297 2467 2662 3605 3622 3775 3960
Size: 1255 717 170 195 943 17 153 185
Cuts at: 3960 4405 325
Size: 445 1498
Fragments arranged by size:
1498 1255 943 717 445 195 185 170
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3824 5536 96
Size: 274 80 33 51 1443 1847 1712 138
Fragments arranged by size:
1847 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2280 2312 5538 5570 2280
Size: 32 3226 32 2288
Fragments arranged by size:
3226 2288 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2804 1316
Size: 1488 4090
XbaI T'CTAG_A
Cuts at: 2914 2914
Size: 5578
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5578
XmaI C'CCGG_G
Cuts at: 1870 2903 1870
Size: 1033 4545
XmnI GAAnn'nnTTC
Cuts at: 2390 3707 2390
Size: 1317 4261
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2806 3386 276
Size: 53 83 186 936 1272 580 2468
Fragments arranged by size:
2468 1272 936 580 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI