Restriction map for
pFN21AB6344
(Circular) (Six-base) MAPSORT of: pFN21AB6344.seq Check: 2460 from: 1 to: 5578
pFN21AB6344 5578 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3388 278
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2625 2921 1937
Size: 688 296 4594
Fragments arranged by size:
4594 688 296
Acc65I G'GTAC_C
Cuts at: 2899 2899
Size: 5578
AclI AA'CG_TT
Cuts at: 1563 3705 4078 1563
Size: 2142 373 3063
Fragments arranged by size:
3063 2142 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 3641 5025 5293 1632
Size: 527 1482 1384 268 1917
Fragments arranged by size:
1917 1482 1384 527 268
AfeI AGC'GCT
Cuts at: 2381 2381
Size: 5578
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5381
AflIII A'CryG_T
Cuts at: 1203 4477 5454 1203
Size: 3274 977 1327
Fragments arranged by size:
3274 1327 977
AgeI A'CCGG_T
Cuts at: 4409 4409
Size: 5578
AhdI GACnn_n'nnGTC
Cuts at: 4307 4307
Size: 5578
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5578
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5578
AlwNI CAG_nnn'CTG
Cuts at: 2169 2842 3321 4893 2169
Size: 673 479 1572 2854
Fragments arranged by size:
2854 1572 673 479
ApaLI G'TGCA_C
Cuts at: 2254 3635 4791 2254
Size: 1381 1156 3041
Fragments arranged by size:
3041 1381 1156
ApoI r'AATT_y
Cuts at: 1493 2552 2887 3113 3265 5374 1493
Size: 1059 335 226 152 2109 1697
Fragments arranged by size:
2109 1697 1059 335 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4132 160
Size: 872 3100 1606
Fragments arranged by size:
3100 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
AvaI C'yCGr_G
Cuts at: 1870 1943 2466 2903 1870
Size: 73 523 437 4545
Fragments arranged by size:
4545 523 437 73
BamHI G'GATC_C
Cuts at: 2908 3275 2908
Size: 367 5211
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2146 2546 2899 4354 618
Size: 325 217 627 359 400 353 1455 1842
Fragments arranged by size:
1842 1455 627 400 359 353 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2897 1391
Size: 622 884 4072
Fragments arranged by size:
4072 884 622
BbeI G_GCGC'C
Cuts at: 2150 2150
Size: 5578
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2165 928
Size: 971 266 4341
Fragments arranged by size:
4341 971 266
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3769 3803 1903
Size: 34 1832 34 3678
Fragments arranged by size:
3678 1832 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3470 4680 3470
Size: 1210 4368
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5578
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2030 4189 136
Size: 107 122 71 631 605 358 2159 1525
Fragments arranged by size:
2159 1525 631 605 358 122 107 71
BglII A'GATC_T
Cuts at: 5573 5573
Size: 5578
BlpI GC'TnA_GC
Cuts at: 2995 2995
Size: 5578
Bme1580I G_kGCm'C
Cuts at: 1790 2258 3639 4795 1790
Size: 468 1381 1156 2573
Fragments arranged by size:
2573 1381 1156 468
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5578
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2864 3309 4267
Size: 416 53 83 399 24 1447 445 958
Cuts at: 4267 442
Size: 1753
Fragments arranged by size:
1753 1447 958 445 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5578
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2678 2678
Size: 5578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2594 2819 4238 1239
Size: 836 519 225 1419 2579
Fragments arranged by size:
2579 1419 836 519 225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3703 4568 4866 3703
Size: 865 298 4415
Fragments arranged by size:
4415 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4241 882
Size: 617 2742 2219
Fragments arranged by size:
2742 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3369 493
Size: 711 2165 2702
Fragments arranged by size:
2702 2165 711
BsaBI GATnn'nnATC
Cuts at: 5572 5572
Size: 5578
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2147 3385 3767 4402
Size: 53 83 186 936 614 1238 382 635
Cuts at: 4402 275
Size: 1451
Fragments arranged by size:
1451 1238 936 635 614 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2310 4011 4409 4683 4830 5241 2310
Size: 1701 398 274 147 411 2647
Fragments arranged by size:
2647 1701 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5353
Fragments arranged by size:
5353 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 1186
Size: 943 4635
BseYI C'CCAG_C
Cuts at: 2097 2435 2491 4781 5277 2097
Size: 338 56 2290 496 2398
Fragments arranged by size:
2398 2290 496 338 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2807 1163
Size: 573 1071 3934
Fragments arranged by size:
3934 1071 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3028 3789 4817 1540
Size: 275 186 1027 761 1028 2301
Fragments arranged by size:
2301 1028 1027 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2258 3639 3724 4795 2258
Size: 1381 85 1071 3041
Fragments arranged by size:
3041 1381 1071 85
BsiWI C'GTAC_G
Cuts at: 2457 2457
Size: 5578
BsmI GAATG_Cn'
Cuts at: 2518 3089 3182 2518
Size: 571 93 4914
Fragments arranged by size:
4914 571 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2858 2858
Size: 5578
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2258 2897 3639 3724 4795 1391
Size: 399 223 245 639 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 639 399 245 223 85
BspEI T'CCGG_A
Cuts at: 2310 5241 2310
Size: 2931 2647
BspHI T'CATG_A
Cuts at: 2742 3467 5197 2742
Size: 725 1730 3123
Fragments arranged by size:
3123 1730 725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2933 844
Size: 2089 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2715 3465 1394
Size: 429 892 750 3507
Fragments arranged by size:
3507 892 750 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4073 4247 65
Size: 1183 2825 174 1396
Fragments arranged by size:
2825 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2225 4222 4409 1700
Size: 110 415 1997 187 2869
Fragments arranged by size:
2869 1997 415 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5578
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5578
BssSI C'ACGA_G
Cuts at: 3638 4650 3638
Size: 1012 4566
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2238 2383 2805 794
Size: 1444 145 422 3567
Fragments arranged by size:
3567 1444 422 145
BstBI TT'CG_AA
Cuts at: 4382 4382
Size: 5578
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5578
BstYI r'GATC_y
Cuts at: 1972 2322 2908 3275 3660 3677 5129 5215 5227
Size: 350 586 367 385 17 1452 86 12
Cuts at: 5227 5573 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 586 385 367 350 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2326 2560 2700 513
Size: 552 939 322 234 140 3391
Fragments arranged by size:
3391 939 552 322 234 140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4839
Fragments arranged by size:
4839 683 56
BtsI GCAGTG_nn'
Cuts at: 2291 3094 3888 3908 2291
Size: 803 794 20 3961
Fragments arranged by size:
3961 803 794 20
ClaI AT'CG_AT
Cuts at: 1554 2421 1554
Size: 867 4711
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5543
DraI TTT'AAA
Cuts at: 2882 2882
Size: 5578
DraIII CAC_nnn'GTG
Cuts at: 2509 2509
Size: 5578
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2621 4585 809
Size: 507 1305 1964 1802
Fragments arranged by size:
1964 1802 1305 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3025 3914 5543
Size: 54 1425 50 275 18 1195 889 1629
Cuts at: 5543 8
Size: 43
Fragments arranged by size:
1629 1425 1195 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3025 1537
Size: 275 1213 4090
Fragments arranged by size:
4090 1213 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2447 3508 1353
Size: 1094 1061 3423
Fragments arranged by size:
3423 1094 1061
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2087 2609 4165 4539 4685 387
Size: 1064 138 498 522 1556 374 146 1280
Fragments arranged by size:
1556 1280 1064 522 498 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2159 2594 2819 3641 4238 5025
Size: 393 443 84 435 225 822 597 787
Cuts at: 5025 5293 1239
Size: 268 1524
Fragments arranged by size:
1524 822 787 597 443 435 393 268
225 84
EcoNI CCTnn'n_nnAGG
Cuts at: 2814 2814
Size: 5578
EcoO109I rG'GnC_Cy
Cuts at: 2305 2305
Size: 5578
EcoRI G'AATT_C
Cuts at: 2887 3265 2887
Size: 378 5200
FspI TGC'GCA
Cuts at: 4084 4084
Size: 5578
HaeII r_GCGC'y
Cuts at: 1678 2150 2383 4725 1678
Size: 472 233 2342 2531
Fragments arranged by size:
2531 2342 472 233
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4219 4251 4293 4325 1373
Size: 32 135 32 2647 32 42 32 2626
Fragments arranged by size:
2647 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2922 3168 669
Size: 988 281 984 246 3079
Fragments arranged by size:
3079 988 984 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5578
HpaI GTT'AAC
Cuts at: 3168 3168
Size: 5578
KasI G'GCGC_C
Cuts at: 2146 2146
Size: 5578
KpnI G_GTAC'C
Cuts at: 2903 2903
Size: 5578
MfeI C'AATT_G
Cuts at: 3177 3177
Size: 5578
MluI A'CGCG_T
Cuts at: 5454 5454
Size: 5578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4692 4876 1305
Size: 3387 184 2007
Fragments arranged by size:
3387 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3756
Fragments arranged by size:
3756 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2421 3536 3895 4054 518
Size: 648 1255 1115 359 159 2042
Fragments arranged by size:
2042 1255 1115 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2491 2519 2994 3671 4819 5064 1606
Size: 885 28 475 677 1148 245 2120
Fragments arranged by size:
2120 1148 885 677 475 245 28
NaeI GCC'GGC
Cuts at: 2227 2227
Size: 5578
NarI GG'CG_CC
Cuts at: 2147 2147
Size: 5578
NcoI C'CATG_G
Cuts at: 513 1065 2004 2326 2700 513
Size: 552 939 322 374 3391
Fragments arranged by size:
3391 939 552 374 322
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5578
NgoMIV G'CCGG_C
Cuts at: 2225 2225
Size: 5578
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5578
NotI GC'GGCC_GC
Cuts at: 3025 3025
Size: 5578
NspI r_CATG'y
Cuts at: 1132 1229 2936 4481 1132
Size: 97 1707 1545 2229
Fragments arranged by size:
2229 1707 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5578
PciI A'CATG_T
Cuts at: 4477 4477
Size: 5578
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2284 2842 1108
Size: 1176 558 3844
Fragments arranged by size:
3844 1176 558
PmeI GTTT'AAAC
Cuts at: 2882 2882
Size: 5578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 5160 5192 3647
Size: 32 1481 32 4033
Fragments arranged by size:
4033 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2305 2305
Size: 5578
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5578
PsiI TTA'TAA
Cuts at: 3148 3148
Size: 5578
PstI C_TGCA'G
Cuts at: 830 2298 2379 2524 2930 830
Size: 1468 81 145 406 3478
Fragments arranged by size:
3478 1468 406 145 81
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5578
PvuII CAG'CTG
Cuts at: 2491 2491
Size: 5578
SalI G'TCGA_C
Cuts at: 1936 2920 1936
Size: 984 4594
SbfI CC_TGCA'GG
Cuts at: 2930 2930
Size: 5578
ScaI AGT'ACT
Cuts at: 2579 3826 5538 2579
Size: 1247 1712 2619
Fragments arranged by size:
2619 1712 1247
SfcI C'TryA_G
Cuts at: 826 1045 2294 2375 2520 2926 4061 4742 4933
Size: 219 1249 81 145 406 1135 681 191
Cuts at: 4933 826
Size: 1471
Fragments arranged by size:
1471 1249 1135 681 406 219 191 145
81
SfoI GGC'GCC
Cuts at: 2148 2148
Size: 5578
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5578
SmaI CCC'GGG
Cuts at: 1872 2468 2905 1872
Size: 596 437 4545
Fragments arranged by size:
4545 596 437
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3682 4583 4845 820
Size: 197 926 1739 901 262 1553
Fragments arranged by size:
1739 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3369 493
Size: 2876 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5578
SphI G_CATG'C
Cuts at: 1132 2936 1132
Size: 1804 3774
SspI AAT'ATT
Cuts at: 5 52 3502 5
Size: 47 3450 2081
Fragments arranged by size:
3450 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5578
StyI C'CwwG_G
Cuts at: 513 1065 2004 2326 2674 2700 3017 513
Size: 552 939 322 348 26 317 3074
Fragments arranged by size:
3074 939 552 348 322 317 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2516 3605 3622 3775 3960 4405 325
Size: 1255 936 1089 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1089 936 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2577 3824 5536
Size: 274 80 33 51 1443 600 1247 1712
Cuts at: 5536 96
Size: 138
Fragments arranged by size:
1712 1443 1247 600 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5538 5570 5538
Size: 32 5546
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5578
XbaI T'CTAG_A
Cuts at: 2914 2914
Size: 5578
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2443 2728 2443
Size: 285 5293
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5578
XmaI C'CCGG_G
Cuts at: 1870 2466 2903 1870
Size: 596 437 4545
Fragments arranged by size:
4545 596 437
XmnI GAAnn'nnTTC
Cuts at: 3707 3707
Size: 5578
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3386 276
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI