Restriction map for
pFN21AB6338
(Circular) (Six-base) MAPSORT of: pFN21AB6338.seq Check: 5762 from: 1 to: 5575
pFN21AB6338 5575 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3385 278
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2355 2918 1937
Size: 418 563 4594
Fragments arranged by size:
4594 563 418
Acc65I G'GTAC_C
Cuts at: 2730 2896 2730
Size: 166 5409
AclI AA'CG_TT
Cuts at: 1563 3702 4075 1563
Size: 2139 373 3063
Fragments arranged by size:
3063 2139 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2193 2267 2643 3638 5022 5290 1632
Size: 561 74 376 995 1384 268 1917
Fragments arranged by size:
1917 1384 995 561 376 268 74
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5378
AflIII A'CryG_T
Cuts at: 1203 4474 5451 1203
Size: 3271 977 1327
Fragments arranged by size:
3271 1327 977
AgeI A'CCGG_T
Cuts at: 4406 4406
Size: 5575
AhdI GACnn_n'nnGTC
Cuts at: 4304 4304
Size: 5575
AleI CACnn'nnGTG
Cuts at: 1166 2101 1166
Size: 935 4640
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5575
AlwNI CAG_nnn'CTG
Cuts at: 2666 3318 4890 2666
Size: 652 1572 3351
Fragments arranged by size:
3351 1572 652
ApaLI G'TGCA_C
Cuts at: 3632 4788 3632
Size: 1156 4419
ApoI r'AATT_y
Cuts at: 1493 2843 2884 3110 3262 5371 1493
Size: 1350 41 226 152 2109 1697
Fragments arranged by size:
2109 1697 1350 226 152 41
AseI AT'TA_AT
Cuts at: 160 1032 4129 160
Size: 872 3097 1606
Fragments arranged by size:
3097 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
AvaI C'yCGr_G
Cuts at: 1870 1943 2225 2326 2900 1870
Size: 73 282 101 574 4545
Fragments arranged by size:
4545 574 282 101 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2714 2747 2714
Size: 33 5542
BamHI G'GATC_C
Cuts at: 2905 3272 2905
Size: 367 5208
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2376 2730 2896 4351 618
Size: 325 217 627 589 354 166 1455 1842
Fragments arranged by size:
1842 1455 627 589 354 325 217 166
BanII G_rGCy'C
Cuts at: 1391 2073 2894 1391
Size: 682 821 4072
Fragments arranged by size:
4072 821 682
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2481 2649 2807 928
Size: 971 582 168 158 3696
Fragments arranged by size:
3696 971 582 168 158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3766 3800 1903
Size: 34 1829 34 3678
Fragments arranged by size:
3678 1829 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3467 4677 3467
Size: 1210 4365
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5575
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4186 136
Size: 107 122 71 631 605 2514 1525
Fragments arranged by size:
2514 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2176 5570 2176
Size: 3394 2181
BlpI GC'TnA_GC
Cuts at: 2992 2992
Size: 5575
Bme1580I G_kGCm'C
Cuts at: 1790 3636 4792 1790
Size: 1846 1156 2573
Fragments arranged by size:
2573 1846 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5575
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3306 4264 442
Size: 416 53 83 399 24 1889 958 1753
Fragments arranged by size:
1889 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2222 2429 4235 1239
Size: 845 138 207 1806 2579
Fragments arranged by size:
2579 1806 845 207 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2299 2670 2850 3700 4565 4863 2299
Size: 371 180 850 865 298 3011
Fragments arranged by size:
3011 865 850 371 298 180
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4238 882
Size: 617 2739 2219
Fragments arranged by size:
2739 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3366 493
Size: 711 2162 2702
Fragments arranged by size:
2702 2162 711
BsaBI GATnn'nnATC
Cuts at: 5569 5569
Size: 5575
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3382 3764 4399 275
Size: 53 83 186 936 1849 382 635 1451
Fragments arranged by size:
1849 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2073 4008 4406 4680 4827 5238 2073
Size: 1935 398 274 147 411 2410
Fragments arranged by size:
2410 1935 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2600 2630 1178
Size: 30 165 30 1197 30 4123
Fragments arranged by size:
4123 1197 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 1186
Size: 931 4644
BseYI C'CCAG_C
Cuts at: 2018 4778 5274 2018
Size: 2760 496 2319
Fragments arranged by size:
2760 2319 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2432 1163
Size: 573 696 4306
Fragments arranged by size:
4306 696 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3025 3786 4814 1540
Size: 275 186 1024 761 1028 2301
Fragments arranged by size:
2301 1028 1024 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2226 3636 3721 4792 2226
Size: 1410 85 1071 3009
Fragments arranged by size:
3009 1410 1071 85
BsmI GAATG_Cn'
Cuts at: 3086 3179 3086
Size: 93 5482
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2226 2894 3636 3721 4792 1391
Size: 399 283 153 668 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 668 399 283 153 85
BspEI T'CCGG_A
Cuts at: 2073 5238 2073
Size: 3165 2410
BspHI T'CATG_A
Cuts at: 3464 5194 3464
Size: 1730 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2930 844
Size: 2086 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3462 1394
Size: 429 1639 3507
Fragments arranged by size:
3507 1639 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4070 4244 65
Size: 1183 2822 174 1396
Fragments arranged by size:
2822 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4219 4406 1700
Size: 110 2409 187 2869
Fragments arranged by size:
2869 2409 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5575
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5575
BssSI C'ACGA_G
Cuts at: 3635 4647 3635
Size: 1012 4563
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5575
BstBI TT'CG_AA
Cuts at: 2812 4379 2812
Size: 1567 4008
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5575
BstYI r'GATC_y
Cuts at: 1972 2176 2905 3272 3657 3674 5126 5212 5224
Size: 204 729 367 385 17 1452 86 12
Cuts at: 5224 5570 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 729 385 367 346 204 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2271 2489 2271
Size: 218 5357
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4084
Fragments arranged by size:
4084 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4836
Fragments arranged by size:
4836 683 56
BtsI GCAGTG_nn'
Cuts at: 2511 2703 2778 3091 3885 3905 2511
Size: 192 75 313 794 20 4181
Fragments arranged by size:
4181 794 313 192 75 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2172 2207 529
Size: 35 1608 35 3897
Fragments arranged by size:
3897 1608 35 35
DraI TTT'AAA
Cuts at: 2848 2879 2848
Size: 31 5544
DraIII CAC_nnn'GTG
Cuts at: 2289 2289
Size: 5575
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4582 809
Size: 507 3266 1802
Fragments arranged by size:
3266 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3022 3911 5540
Size: 54 1425 50 275 18 1192 889 1629
Cuts at: 5540 8
Size: 43
Fragments arranged by size:
1629 1425 1192 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3022 1537
Size: 275 1210 4090
Fragments arranged by size:
4090 1210 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3505 1353
Size: 2152 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4162 4536 4682 387
Size: 1064 138 2573 374 146 1280
Fragments arranged by size:
2573 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2193 2222 2267 2429 2643 3638
Size: 393 452 109 29 45 162 214 995
Cuts at: 3638 4235 5022 5290 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 995 787 597 452 393 268 214
162 109 45 29
EcoO109I rG'GnC_Cy
Cuts at: 2449 2860 2449
Size: 411 5164
EcoRI G'AATT_C
Cuts at: 2884 3262 2884
Size: 378 5197
EcoRV GAT'ATC
Cuts at: 2079 2079
Size: 5575
FspI TGC'GCA
Cuts at: 4081 4081
Size: 5575
HaeII r_GCGC'y
Cuts at: 1678 4722 1678
Size: 3044 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2161 2193 2600 2632 4216
Size: 32 135 32 589 32 407 32 1584
Cuts at: 4216 4248 4290 4322 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1584 589 407 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2356 2703 2919 3165 669
Size: 988 281 418 347 216 246 3079
Fragments arranged by size:
3079 988 418 347 281 246 216
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5575
HpaI GTT'AAC
Cuts at: 3165 3165
Size: 5575
KpnI G_GTAC'C
Cuts at: 2734 2900 2734
Size: 166 5409
MfeI C'AATT_G
Cuts at: 3174 3174
Size: 5575
MluI A'CGCG_T
Cuts at: 5451 5451
Size: 5575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4689 4873 1305
Size: 3384 184 2007
Fragments arranged by size:
3384 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3753
Fragments arranged by size:
3753 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2101 3533 3892 4051 518
Size: 648 935 1432 359 159 2042
Fragments arranged by size:
2042 1432 935 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2122 2159 2502 2613 2991 3668 4816 5061
Size: 516 37 343 111 378 677 1148 245
Cuts at: 5061 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 516 378 343 245 111
37
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4084
Fragments arranged by size:
4084 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5575
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5575
NotI GC'GGCC_GC
Cuts at: 3022 3022
Size: 5575
NspI r_CATG'y
Cuts at: 1132 1229 2167 2933 4478 1132
Size: 97 938 766 1545 2229
Fragments arranged by size:
2229 1545 938 766 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5575
PciI A'CATG_T
Cuts at: 4474 4474
Size: 5575
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2289 1108
Size: 1181 4394
PfoI T'CCnGG_A
Cuts at: 2199 2199
Size: 5575
PmeI GTTT'AAAC
Cuts at: 2879 2879
Size: 5575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3644 3676 5157 5189 3644
Size: 32 1481 32 4030
Fragments arranged by size:
4030 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2860 2860
Size: 5575
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5575
PsiI TTA'TAA
Cuts at: 3145 3145
Size: 5575
PspXI vC'TCGA_Gb
Cuts at: 2326 2326
Size: 5575
PstI C_TGCA'G
Cuts at: 830 2630 2927 830
Size: 1800 297 3478
Fragments arranged by size:
3478 1800 297
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5575
PvuII CAG'CTG
Cuts at: 2122 2159 2502 2122
Size: 37 343 5195
Fragments arranged by size:
5195 343 37
SalI G'TCGA_C
Cuts at: 1936 2354 2917 1936
Size: 418 563 4594
Fragments arranged by size:
4594 563 418
SbfI CC_TGCA'GG
Cuts at: 2927 2927
Size: 5575
ScaI AGT'ACT
Cuts at: 3823 5535 3823
Size: 1712 3863
SfcI C'TryA_G
Cuts at: 826 1045 2626 2923 4058 4739 4930 826
Size: 219 1581 297 1135 681 191 1471
Fragments arranged by size:
1581 1471 1135 681 297 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5575
SmaI CCC'GGG
Cuts at: 1872 2902 1872
Size: 1030 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2225 2314 2326 2685 2865 3679
Size: 197 926 282 89 12 359 180 814
Cuts at: 3679 4580 4842 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 901 814 359 282 262 197
180 89 12
SnaBI TAC'GTA
Cuts at: 493 3366 493
Size: 2873 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5575
SphI G_CATG'C
Cuts at: 1132 2167 2933 1132
Size: 1035 766 3774
Fragments arranged by size:
3774 1035 766
SspI AAT'ATT
Cuts at: 5 52 3499 5
Size: 47 3447 2081
Fragments arranged by size:
3447 2081 47
StuI AGG'CCT
Cuts at: 1517 2324 1517
Size: 807 4768
StyI C'CwwG_G
Cuts at: 513 1065 2004 3014 513
Size: 552 939 1010 3074
Fragments arranged by size:
3074 1010 939 552
SwaI ATTT'AAAT
Cuts at: 2848 2848
Size: 5575
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 2449 3602 3619 3772 3957 4402
Size: 1255 417 452 1153 17 153 185 445
Cuts at: 4402 325
Size: 1498
Fragments arranged by size:
1498 1255 1153 452 445 417 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2370 3821 5533
Size: 274 80 33 51 1443 393 1451 1712
Cuts at: 5533 96
Size: 138
Fragments arranged by size:
1712 1451 1443 393 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5535 5567 5535
Size: 32 5543
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5575
XbaI T'CTAG_A
Cuts at: 2911 2911
Size: 5575
XhoI C'TCGA_G
Cuts at: 1943 2225 2326 1943
Size: 282 101 5192
Fragments arranged by size:
5192 282 101
XmaI C'CCGG_G
Cuts at: 1870 2900 1870
Size: 1030 4545
XmnI GAAnn'nnTTC
Cuts at: 3704 3704
Size: 5575
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3383 276
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI