Restriction map for
pFN21AB6330
(Circular) (Six-base) MAPSORT of: pFN21AB6330.seq Check: 5471 from: 1 to: 5569
pFN21AB6330 5569 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2272 2272
Size: 5569
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2760 3379 278
Size: 53 83 186 936 1224 619 2468
Fragments arranged by size:
2468 1224 936 619 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2912 1937
Size: 975 4594
Acc65I G'GTAC_C
Cuts at: 2890 2890
Size: 5569
AclI AA'CG_TT
Cuts at: 1563 3696 4069 1563
Size: 2133 373 3063
Fragments arranged by size:
3063 2133 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2024 3632 5016 5284 1632
Size: 392 1608 1384 268 1917
Fragments arranged by size:
1917 1608 1384 392 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5372
AflIII A'CryG_T
Cuts at: 1203 4468 5445 1203
Size: 3265 977 1327
Fragments arranged by size:
3265 1327 977
AgeI A'CCGG_T
Cuts at: 4400 4400
Size: 5569
AhdI GACnn_n'nnGTC
Cuts at: 4298 4298
Size: 5569
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5569
AlwNI CAG_nnn'CTG
Cuts at: 2089 2287 2584 3312 4884 2089
Size: 198 297 728 1572 2774
Fragments arranged by size:
2774 1572 728 297 198
ApaLI G'TGCA_C
Cuts at: 3626 4782 3626
Size: 1156 4413
ApoI r'AATT_y
Cuts at: 1493 2878 3104 3256 5365 1493
Size: 1385 226 152 2109 1697
Fragments arranged by size:
2109 1697 1385 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4123 160
Size: 872 3091 1606
Fragments arranged by size:
3091 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
AvaI C'yCGr_G
Cuts at: 1870 1943 2102 2894 1870
Size: 73 159 792 4545
Fragments arranged by size:
4545 792 159 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2805 2838 2805
Size: 33 5536
BamHI G'GATC_C
Cuts at: 2217 2899 3266 2217
Size: 682 367 4520
Fragments arranged by size:
4520 682 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2890 4345 618
Size: 325 217 627 1103 1455 1842
Fragments arranged by size:
1842 1455 1103 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2103 2243 2888 1391
Size: 712 140 645 4072
Fragments arranged by size:
4072 712 645 140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2842 928
Size: 971 943 3655
Fragments arranged by size:
3655 971 943
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3760 3794 1903
Size: 34 1823 34 3678
Fragments arranged by size:
3678 1823 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3461 4671 3461
Size: 1210 4359
BclI T'GATC_A
Cuts at: 1548 2250 1548
Size: 702 4867
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2595 4180 136
Size: 107 122 71 631 605 923 1585 1525
Fragments arranged by size:
1585 1525 923 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2478 5564 2478
Size: 3086 2483
BlpI GC'TnA_GC
Cuts at: 2277 2577 2986 2277
Size: 300 409 4860
Fragments arranged by size:
4860 409 300
Bme1580I G_kGCm'C
Cuts at: 1790 2142 3630 4786 1790
Size: 352 1488 1156 2573
Fragments arranged by size:
2573 1488 1156 352
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5569
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2624 3300 4258
Size: 416 53 83 399 24 1207 676 958
Cuts at: 4258 442
Size: 1753
Fragments arranged by size:
1753 1207 958 676 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5569
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2661 2661
Size: 5569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2783 4229 1239
Size: 1544 1446 2579
Fragments arranged by size:
2579 1544 1446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2045 3694 4559 4857 2045
Size: 1649 865 298 2757
Fragments arranged by size:
2757 1649 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4232 882
Size: 617 2733 2219
Fragments arranged by size:
2733 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3360 493
Size: 711 2156 2702
Fragments arranged by size:
2702 2156 711
BsaBI GATnn'nnATC
Cuts at: 5563 5563
Size: 5569
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2757 3376 3758 4393
Size: 53 83 186 936 1224 619 382 635
Cuts at: 4393 275
Size: 1451
Fragments arranged by size:
1451 1224 936 635 619 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4002 4400 4674 4821 5232 4002
Size: 398 274 147 411 4339
Fragments arranged by size:
4339 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5344
Fragments arranged by size:
5344 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2333 2468 2471 1186
Size: 874 273 135 3 4284
Fragments arranged by size:
4284 874 273 135 3
BseYI C'CCAG_C
Cuts at: 2172 2551 4772 5268 2172
Size: 379 2221 496 2473
Fragments arranged by size:
2473 2221 496 379
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2357 1163
Size: 573 621 4375
Fragments arranged by size:
4375 621 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3019 3780 4808 1540
Size: 275 186 1018 761 1028 2301
Fragments arranged by size:
2301 1028 1018 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2103 2304 2718 3630 3715 4786 2103
Size: 201 414 912 85 1071 2886
Fragments arranged by size:
2886 1071 912 414 201 85
BsmI GAATG_Cn'
Cuts at: 3080 3173 3080
Size: 93 5476
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2142 2243 2304 2718 2888 3630
Size: 399 313 39 101 61 414 170 742
Cuts at: 3630 3715 4786 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 414 399 313 170 101
85 61 39
BspEI T'CCGG_A
Cuts at: 5232 5232
Size: 5569
BspHI T'CATG_A
Cuts at: 3458 5188 3458
Size: 1730 3839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2088 2272 2329 2924 844
Size: 1244 184 57 595 3489
Fragments arranged by size:
3489 1244 595 184 57
BsrBI CCG'CTC
Cuts at: 1394 1823 2703 3456 1394
Size: 429 880 753 3507
Fragments arranged by size:
3507 880 753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4064 4238 65
Size: 1183 2816 174 1396
Fragments arranged by size:
2816 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2325 4213 4400 1700
Size: 110 515 1888 187 2869
Fragments arranged by size:
2869 1888 515 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5569
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5569
BssSI C'ACGA_G
Cuts at: 3629 4641 3629
Size: 1012 4557
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2287 794
Size: 1493 4076
BstBI TT'CG_AA
Cuts at: 4373 4373
Size: 5569
BstEII G'GTnAC_C
Cuts at: 1184 2365 1184
Size: 1181 4388
BstXI CCAn_nnnn'nTGG
Cuts at: 2402 2402
Size: 5569
BstYI r'GATC_y
Cuts at: 1972 2145 2217 2478 2664 2766 2899 3266 3651
Size: 173 72 261 186 102 133 367 385
Cuts at: 3651 3668 5120 5206 5218 5564 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 261 186 173
133 102 86 72 17 12
Bsu36I CC'TnA_GG
Cuts at: 2441 2441
Size: 5569
BtgI C'CryG_G
Cuts at: 513 1065 2361 2788 513
Size: 552 1296 427 3294
Fragments arranged by size:
3294 1296 552 427
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2526 487
Size: 683 56 1300 3530
Fragments arranged by size:
3530 1300 683 56
BtsI GCAGTG_nn'
Cuts at: 2221 3085 3879 3899 2221
Size: 864 794 20 3891
Fragments arranged by size:
3891 864 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5534
DraI TTT'AAA
Cuts at: 2873 2873
Size: 5569
DraIII CAC_nnn'GTG
Cuts at: 2618 2618
Size: 5569
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4576 809
Size: 507 3260 1802
Fragments arranged by size:
3260 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2404 2596 3016
Size: 54 1425 50 275 18 574 192 420
Cuts at: 3016 3905 5534 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 574 420 275 192 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3016 1537
Size: 275 1204 4090
Fragments arranged by size:
4090 1204 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3499 1353
Size: 2146 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4156 4530 4676 387
Size: 1064 138 2567 374 146 1280
Fragments arranged by size:
2567 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2101 2101
Size: 5569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2024 2783 3632 4229 5016 5284 1239
Size: 393 392 759 849 597 787 268 1524
Fragments arranged by size:
1524 849 787 759 597 393 392 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2042 2042
Size: 5569
EcoO109I rG'GnC_Cy
Cuts at: 2077 2125 2179 2809 2839 2077
Size: 48 54 630 30 4807
Fragments arranged by size:
4807 630 54 48 30
EcoRI G'AATT_C
Cuts at: 2878 3256 2878
Size: 378 5191
EcoRV GAT'ATC
Cuts at: 2731 2731
Size: 5569
FspI TGC'GCA
Cuts at: 4075 4075
Size: 5569
HaeII r_GCGC'y
Cuts at: 1678 4716 1678
Size: 3038 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2669 2701 4210 4242 4284
Size: 32 135 32 1097 32 1509 32 42
Cuts at: 4284 4316 1373
Size: 32 2626
Fragments arranged by size:
2626 1509 1097 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2913 3159 669
Size: 988 281 975 246 3079
Fragments arranged by size:
3079 988 975 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5569
HpaI GTT'AAC
Cuts at: 3159 3159
Size: 5569
KpnI G_GTAC'C
Cuts at: 2894 2894
Size: 5569
MfeI C'AATT_G
Cuts at: 3168 3168
Size: 5569
MluI A'CGCG_T
Cuts at: 5445 5445
Size: 5569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4683 4867 1305
Size: 3378 184 2007
Fragments arranged by size:
3378 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3747
Fragments arranged by size:
3747 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2378 3527 3886 4045 518
Size: 648 1212 1149 359 159 2042
Fragments arranged by size:
2042 1212 1149 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2790 2985 3662 4810 5055 1606
Size: 483 701 195 677 1148 245 2120
Fragments arranged by size:
2120 1148 701 677 483 245 195
NaeI GCC'GGC
Cuts at: 2327 2327
Size: 5569
NcoI C'CATG_G
Cuts at: 513 1065 2361 513
Size: 552 1296 3721
Fragments arranged by size:
3721 1296 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5569
NgoMIV G'CCGG_C
Cuts at: 2325 2325
Size: 5569
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5569
NotI GC'GGCC_GC
Cuts at: 3016 3016
Size: 5569
NspI r_CATG'y
Cuts at: 1132 1229 2927 4472 1132
Size: 97 1698 1545 2229
Fragments arranged by size:
2229 1698 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5569
PciI A'CATG_T
Cuts at: 4468 4468
Size: 5569
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2350 2383 2618 1108
Size: 1242 33 235 4059
Fragments arranged by size:
4059 1242 235 33
PfoI T'CCnGG_A
Cuts at: 2760 2760
Size: 5569
PmeI GTTT'AAAC
Cuts at: 2873 2873
Size: 5569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 5151 5183 3638
Size: 32 1481 32 4024
Fragments arranged by size:
4024 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2077 2125 2179 2077
Size: 48 54 5467
Fragments arranged by size:
5467 54 48
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5569
PsiI TTA'TAA
Cuts at: 3139 3139
Size: 5569
PstI C_TGCA'G
Cuts at: 830 2085 2921 830
Size: 1255 836 3478
Fragments arranged by size:
3478 1255 836
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5569
PvuII CAG'CTG
Cuts at: 2089 2089
Size: 5569
SacI G_AGCT'C
Cuts at: 2103 2103
Size: 5569
SacII CC_GC'GG
Cuts at: 2791 2791
Size: 5569
SalI G'TCGA_C
Cuts at: 1936 2911 1936
Size: 975 4594
SbfI CC_TGCA'GG
Cuts at: 2921 2921
Size: 5569
ScaI AGT'ACT
Cuts at: 3817 5529 3817
Size: 1712 3857
SfcI C'TryA_G
Cuts at: 826 1045 2081 2917 4052 4733 4924 826
Size: 219 1036 836 1135 681 191 1471
Fragments arranged by size:
1471 1135 1036 836 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5569
SmaI CCC'GGG
Cuts at: 1872 2896 1872
Size: 1024 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2060 3673 4574 4836 820
Size: 197 926 117 1613 901 262 1553
Fragments arranged by size:
1613 1553 926 901 262 197 117
SnaBI TAC'GTA
Cuts at: 493 3360 493
Size: 2867 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5569
SphI G_CATG'C
Cuts at: 1132 2927 1132
Size: 1795 3774
SspI AAT'ATT
Cuts at: 5 52 3493 5
Size: 47 3441 2081
Fragments arranged by size:
3441 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5569
StyI C'CwwG_G
Cuts at: 513 1065 2349 2361 2827 3008 513
Size: 552 1284 12 466 181 3074
Fragments arranged by size:
3074 1284 552 466 181 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2186 2606 3596 3613 3766 3951 4396
Size: 1255 606 420 990 17 153 185 445
Cuts at: 4396 325
Size: 1498
Fragments arranged by size:
1498 1255 990 606 445 420 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3815 5527 96
Size: 274 80 33 51 1443 1838 1712 138
Fragments arranged by size:
1838 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2413 2445 2491 2523 5529 5561 2413
Size: 32 46 32 3006 32 2421
Fragments arranged by size:
3006 2421 46 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2123 1316
Size: 807 4762
XbaI T'CTAG_A
Cuts at: 2905 2905
Size: 5569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2149 2380 2149
Size: 231 5338
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5569
XmaI C'CCGG_G
Cuts at: 1870 2894 1870
Size: 1024 4545
XmnI GAAnn'nnTTC
Cuts at: 3698 3698
Size: 5569
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2758 3377 276
Size: 53 83 186 936 1224 619 2468
Fragments arranged by size:
2468 1224 936 619 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI