Restriction map for
pFN21AB6324
(Circular) (Six-base) MAPSORT of: pFN21AB6324.seq Check: 6474 from: 1 to: 5551
pFN21AB6324 5551 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3361 278
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2894 1937
Size: 957 4594
Acc65I G'GTAC_C
Cuts at: 2872 2872
Size: 5551
AclI AA'CG_TT
Cuts at: 1563 2073 3678 4051 1563
Size: 510 1605 373 3063
Fragments arranged by size:
3063 1605 510 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3614 4998 5266 1632
Size: 1982 1384 268 1917
Fragments arranged by size:
1982 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5354
AflIII A'CryG_T
Cuts at: 1203 2126 4450 5427 1203
Size: 923 2324 977 1327
Fragments arranged by size:
2324 1327 977 923
AgeI A'CCGG_T
Cuts at: 4382 4382
Size: 5551
AhdI GACnn_n'nnGTC
Cuts at: 4280 4280
Size: 5551
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5551
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2205 1184
Size: 1021 4530
AlwNI CAG_nnn'CTG
Cuts at: 3294 4866 3294
Size: 1572 3979
ApaLI G'TGCA_C
Cuts at: 3608 4764 3608
Size: 1156 4395
ApoI r'AATT_y
Cuts at: 1493 2860 3086 3238 5347 1493
Size: 1367 226 152 2109 1697
Fragments arranged by size:
2109 1697 1367 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2256 2463 4105 160
Size: 872 1224 207 1642 1606
Fragments arranged by size:
1642 1606 1224 872 207
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
AvaI C'yCGr_G
Cuts at: 1870 1943 2876 1870
Size: 73 933 4545
Fragments arranged by size:
4545 933 73
BamHI G'GATC_C
Cuts at: 2881 3248 2881
Size: 367 5184
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2872 4327 618
Size: 325 217 627 1085 1455 1842
Fragments arranged by size:
1842 1455 1085 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2493 2870 1391
Size: 1102 377 4072
Fragments arranged by size:
4072 1102 377
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4580
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3742 3776 1903
Size: 34 50 34 1721 34 3678
Fragments arranged by size:
3678 1721 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3443 4653 3443
Size: 1210 4341
BclI T'GATC_A
Cuts at: 1548 2041 1548
Size: 493 5058
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4162 136
Size: 107 122 71 631 605 2490 1525
Fragments arranged by size:
2490 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5546 5546
Size: 5551
BlpI GC'TnA_GC
Cuts at: 2968 2968
Size: 5551
Bme1580I G_kGCm'C
Cuts at: 1790 3612 4768 1790
Size: 1822 1156 2573
Fragments arranged by size:
2573 1822 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5551
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3282 4240 442
Size: 416 53 83 399 24 1865 958 1753
Fragments arranged by size:
1865 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4211 1239
Size: 2972 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3676 4541 4839 3676
Size: 865 298 4388
Fragments arranged by size:
4388 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2420 4214 882
Size: 617 921 1794 2219
Fragments arranged by size:
2219 1794 921 617
BsaAI yAC'GTr
Cuts at: 493 1204 2127 3342 493
Size: 711 923 1215 2702
Fragments arranged by size:
2702 1215 923 711
BsaBI GATnn'nnATC
Cuts at: 5545 5545
Size: 5551
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3358 3740 4375 275
Size: 53 83 186 936 1825 382 635 1451
Fragments arranged by size:
1825 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3984 4382 4656 4803 5214 3984
Size: 398 274 147 411 4321
Fragments arranged by size:
4321 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2373 2403 2533 2563 1178
Size: 30 165 30 970 30 130 30 4166
Fragments arranged by size:
4166 970 165 130 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2397 2438 1186
Size: 1211 41 4299
Fragments arranged by size:
4299 1211 41
BseYI C'CCAG_C
Cuts at: 4754 5250 4754
Size: 496 5055
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2307 1163
Size: 573 571 4407
Fragments arranged by size:
4407 573 571
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3001 3762 4790 1540
Size: 275 186 1000 761 1028 2301
Fragments arranged by size:
2301 1028 1000 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3612 3697 4768 3612
Size: 85 1071 4395
Fragments arranged by size:
4395 1071 85
BsmI GAATG_Cn'
Cuts at: 3062 3155 3062
Size: 93 5458
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2493 2870 3612 3697 4768 1391
Size: 399 703 377 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 703 399 377 85
BspEI T'CCGG_A
Cuts at: 5214 5214
Size: 5551
BspHI T'CATG_A
Cuts at: 3440 5170 3440
Size: 1730 3821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2906 844
Size: 2062 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3438 1394
Size: 429 1615 3507
Fragments arranged by size:
3507 1615 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4046 4220 65
Size: 1183 2798 174 1396
Fragments arranged by size:
2798 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4195 4382 1700
Size: 110 2385 187 2869
Fragments arranged by size:
2869 2385 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5551
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5551
BssSI C'ACGA_G
Cuts at: 3611 4623 3611
Size: 1012 4539
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5551
BstBI TT'CG_AA
Cuts at: 2251 2329 4355 2251
Size: 78 2026 3447
Fragments arranged by size:
3447 2026 78
BstEII G'GTnAC_C
Cuts at: 1184 2351 1184
Size: 1167 4384
BstXI CCAn_nnnn'nTGG
Cuts at: 2348 2348
Size: 5551
BstYI r'GATC_y
Cuts at: 1972 2025 2881 3248 3633 3650 5102 5188 5200
Size: 53 856 367 385 17 1452 86 12
Cuts at: 5200 5546 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 856 385 367 346 86 53
17 12
Bsu36I CC'TnA_GG
Cuts at: 2421 2421
Size: 5551
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4999
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4812
Fragments arranged by size:
4812 683 56
BtsI GCAGTG_nn'
Cuts at: 3067 3861 3881 3067
Size: 794 20 4737
Fragments arranged by size:
4737 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5551
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5516
DraI TTT'AAA
Cuts at: 2224 2855 2224
Size: 631 4920
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4558 809
Size: 507 3242 1802
Fragments arranged by size:
3242 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2998 3887
Size: 54 1425 50 275 18 228 940 889
Cuts at: 3887 5516 8
Size: 1629 43
Fragments arranged by size:
1629 1425 940 889 275 228 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2998 1537
Size: 275 1186 4090
Fragments arranged by size:
4090 1186 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2229 3481 1353
Size: 876 1252 3423
Fragments arranged by size:
3423 1252 876
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4138 4512 4658 387
Size: 1064 138 2549 374 146 1280
Fragments arranged by size:
2549 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3614 4211 4998 5266 1239
Size: 393 1982 597 787 268 1524
Fragments arranged by size:
1982 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2307 2307
Size: 5551
EcoRI G'AATT_C
Cuts at: 2860 3238 2860
Size: 378 5173
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 5551
FspI TGC'GCA
Cuts at: 4057 4057
Size: 5551
HaeII r_GCGC'y
Cuts at: 1678 4698 1678
Size: 3020 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2267 2299 4192 4224 4266
Size: 32 135 32 695 32 1893 32 42
Cuts at: 4266 4298 1373
Size: 32 2626
Fragments arranged by size:
2626 1893 695 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2665 2895 3141 669
Size: 988 281 727 230 246 3079
Fragments arranged by size:
3079 988 727 281 246 230
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5551
HpaI GTT'AAC
Cuts at: 3141 3141
Size: 5551
KpnI G_GTAC'C
Cuts at: 2876 2876
Size: 5551
MfeI C'AATT_G
Cuts at: 3150 3150
Size: 5551
MluI A'CGCG_T
Cuts at: 5427 5427
Size: 5551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4665 4849 1305
Size: 3360 184 2007
Fragments arranged by size:
3360 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2060 10
Size: 54 1425 343 228 3501
Fragments arranged by size:
3501 1425 343 228 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3509 3868 4027 518
Size: 648 2343 359 159 2042
Fragments arranged by size:
2343 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2967 3644 4792 5037 1606
Size: 1361 677 1148 245 2120
Fragments arranged by size:
2120 1361 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4999
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5551
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5551
NotI GC'GGCC_GC
Cuts at: 2998 2998
Size: 5551
NspI r_CATG'y
Cuts at: 1132 1229 2123 2909 4454 1132
Size: 97 894 786 1545 2229
Fragments arranged by size:
2229 1545 894 786 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5551
PciI A'CATG_T
Cuts at: 4450 4450
Size: 5551
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5551
PfoI T'CCnGG_A
Cuts at: 2370 2370
Size: 5551
PmeI GTTT'AAAC
Cuts at: 2855 2855
Size: 5551
PmlI CAC'GTG
Cuts at: 2127 2127
Size: 5551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3620 3652 5133 5165 3620
Size: 32 1481 32 4006
Fragments arranged by size:
4006 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2660 1613
Size: 1047 4504
PsiI TTA'TAA
Cuts at: 3121 3121
Size: 5551
PstI C_TGCA'G
Cuts at: 830 2450 2481 2903 830
Size: 1620 31 422 3478
Fragments arranged by size:
3478 1620 422 31
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5551
SalI G'TCGA_C
Cuts at: 1936 2893 1936
Size: 957 4594
SbfI CC_TGCA'GG
Cuts at: 2903 2903
Size: 5551
ScaI AGT'ACT
Cuts at: 3799 5511 3799
Size: 1712 3839
SfcI C'TryA_G
Cuts at: 826 1045 2446 2477 2899 4034 4715 4906 826
Size: 219 1401 31 422 1135 681 191 1471
Fragments arranged by size:
1471 1401 1135 681 422 219 191 31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5551
SmaI CCC'GGG
Cuts at: 1872 2878 1872
Size: 1006 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3655 4556 4818 820
Size: 197 926 1712 901 262 1553
Fragments arranged by size:
1712 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3342 493
Size: 2849 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5551
SphI G_CATG'C
Cuts at: 1132 2909 1132
Size: 1777 3774
SspI AAT'ATT
Cuts at: 5 52 2211 2810 3475 5
Size: 47 2159 599 665 2081
Fragments arranged by size:
2159 2081 665 599 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5551
StyI C'CwwG_G
Cuts at: 513 1065 2341 2990 513
Size: 552 1276 649 3074
Fragments arranged by size:
3074 1276 649 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3578 3595 3748 3933 4378 325
Size: 1255 1998 17 153 185 445 1498
Fragments arranged by size:
1998 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2046 3797 5509
Size: 274 80 33 51 1443 69 1751 1712
Cuts at: 5509 96
Size: 138
Fragments arranged by size:
1751 1712 1443 274 138 80 69 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2373 2405 5511 5543 2373
Size: 32 3106 32 2381
Fragments arranged by size:
3106 2381 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5551
XbaI T'CTAG_A
Cuts at: 2887 2887
Size: 5551
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5551
XmaI C'CCGG_G
Cuts at: 1870 2876 1870
Size: 1006 4545
XmnI GAAnn'nnTTC
Cuts at: 2640 2832 3680 2640
Size: 192 848 4511
Fragments arranged by size:
4511 848 192
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3359 276
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI