Restriction map for
pFN21AB6321
(Circular) (Six-base) MAPSORT of: pFN21AB6321.seq Check: 3556 from: 1 to: 5488
pFN21AB6321 5488 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3298 278
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2831 1937
Size: 894 4594
Acc65I G'GTAC_C
Cuts at: 2809 2809
Size: 5488
AclI AA'CG_TT
Cuts at: 1563 3615 3988 1563
Size: 2052 373 3063
Fragments arranged by size:
3063 2052 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3551 4935 5203 1632
Size: 1919 1384 268 1917
Fragments arranged by size:
1919 1917 1384 268
AfeI AGC'GCT
Cuts at: 2343 2509 2343
Size: 166 5322
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5291
AflIII A'CryG_T
Cuts at: 1203 4387 5364 1203
Size: 3184 977 1327
Fragments arranged by size:
3184 1327 977
AgeI A'CCGG_T
Cuts at: 4319 4319
Size: 5488
AhdI GACnn_n'nnGTC
Cuts at: 4217 4217
Size: 5488
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5488
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5488
AlwNI CAG_nnn'CTG
Cuts at: 3231 4803 3231
Size: 1572 3916
ApaLI G'TGCA_C
Cuts at: 3545 4701 3545
Size: 1156 4332
ApoI r'AATT_y
Cuts at: 1493 2112 2196 2797 3023 3175 5284 1493
Size: 619 84 601 226 152 2109 1697
Fragments arranged by size:
2109 1697 619 601 226 152 84
AseI AT'TA_AT
Cuts at: 160 1032 4042 160
Size: 872 3010 1606
Fragments arranged by size:
3010 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
AvaI C'yCGr_G
Cuts at: 1870 1943 2813 1870
Size: 73 870 4545
Fragments arranged by size:
4545 870 73
BamHI G'GATC_C
Cuts at: 2818 3185 2818
Size: 367 5121
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2099 2809 4264 618
Size: 325 217 627 312 710 1455 1842
Fragments arranged by size:
1842 1455 710 627 325 312 217
BanII G_rGCy'C
Cuts at: 1391 2807 1391
Size: 1416 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4517
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3679 3713 1903
Size: 34 1742 34 3678
Fragments arranged by size:
3678 1742 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3380 4590 3380
Size: 1210 4278
BclI T'GATC_A
Cuts at: 1548 2712 1548
Size: 1164 4324
BfrBI ATG'CAT
Cuts at: 2493 2493
Size: 5488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4099 136
Size: 107 122 71 631 605 2427 1525
Fragments arranged by size:
2427 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5483 5483
Size: 5488
BlpI GC'TnA_GC
Cuts at: 2745 2905 2745
Size: 160 5328
Bme1580I G_kGCm'C
Cuts at: 1790 3549 4705 1790
Size: 1759 1156 2573
Fragments arranged by size:
2573 1759 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5488
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3219 4177 442
Size: 416 53 83 399 24 1802 958 1753
Fragments arranged by size:
1802 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2008 2028 4148 1239
Size: 769 20 2120 2579
Fragments arranged by size:
2579 2120 769 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3613 4478 4776 3613
Size: 865 298 4325
Fragments arranged by size:
4325 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4151 882
Size: 617 2652 2219
Fragments arranged by size:
2652 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3279 493
Size: 711 2075 2702
Fragments arranged by size:
2702 2075 711
BsaBI GATnn'nnATC
Cuts at: 5482 5482
Size: 5488
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3295 3677 4312 275
Size: 53 83 186 936 1762 382 635 1451
Fragments arranged by size:
1762 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3921 4319 4593 4740 5151 3921
Size: 398 274 147 411 4258
Fragments arranged by size:
4258 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5263
Fragments arranged by size:
5263 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5488
BseYI C'CCAG_C
Cuts at: 2418 4691 5187 2418
Size: 2273 496 2719
Fragments arranged by size:
2719 2273 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4915
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2938 3699 4727 1540
Size: 275 186 937 761 1028 2301
Fragments arranged by size:
2301 1028 937 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3549 3634 4705 3549
Size: 85 1071 4332
Fragments arranged by size:
4332 1071 85
BsmI GAATG_Cn'
Cuts at: 2999 3092 2999
Size: 93 5395
BsmBI CGTCTCn'nnnn_
Cuts at: 2143 2143
Size: 5488
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2807 3549 3634 4705 1391
Size: 399 1017 742 85 1071 2174
Fragments arranged by size:
2174 1071 1017 742 399 85
BspEI T'CCGG_A
Cuts at: 5151 5151
Size: 5488
BspHI T'CATG_A
Cuts at: 3377 5107 3377
Size: 1730 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2843 844
Size: 1999 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3375 1394
Size: 429 1552 3507
Fragments arranged by size:
3507 1552 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2280 2402 3983 4157 65
Size: 1183 1032 122 1581 174 1396
Fragments arranged by size:
1581 1396 1183 1032 174 122
BsrFI r'CCGG_y
Cuts at: 1700 1810 4132 4319 1700
Size: 110 2322 187 2869
Fragments arranged by size:
2869 2322 187 110
BsrGI T'GTAC_A
Cuts at: 96 2409 96
Size: 2313 3175
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5488
BssSI C'ACGA_G
Cuts at: 2723 3548 4560 2723
Size: 825 1012 3651
Fragments arranged by size:
3651 1012 825
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5488
BstBI TT'CG_AA
Cuts at: 4292 4292
Size: 5488
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5488
BstYI r'GATC_y
Cuts at: 1972 2818 3185 3570 3587 5039 5125 5137 5483
Size: 846 367 385 17 1452 86 12 346
Cuts at: 5483 1972
Size: 1977
Fragments arranged by size:
1977 1452 846 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2076 2604 513
Size: 552 1011 528 3397
Fragments arranged by size:
3397 1011 552 528
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4749
Fragments arranged by size:
4749 683 56
BtsI GCAGTG_nn'
Cuts at: 2233 3004 3798 3818 2233
Size: 771 794 20 3903
Fragments arranged by size:
3903 794 771 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2476 2511 529
Size: 35 1912 35 3506
Fragments arranged by size:
3506 1912 35 35
DraI TTT'AAA
Cuts at: 2792 2792
Size: 5488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2563 4495 809
Size: 507 1247 1932 1802
Fragments arranged by size:
1932 1802 1247 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2935 3824 5453
Size: 54 1425 50 275 18 1105 889 1629
Cuts at: 5453 8
Size: 43
Fragments arranged by size:
1629 1425 1105 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2935 1537
Size: 275 1123 4090
Fragments arranged by size:
4090 1123 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3418 1353
Size: 2065 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4075 4449 4595 387
Size: 1064 138 2486 374 146 1280
Fragments arranged by size:
2486 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2008 2028 3551 4148 4935 5203 1239
Size: 393 376 20 1523 597 787 268 1524
Fragments arranged by size:
1524 1523 787 597 393 376 268 20
EcoRI G'AATT_C
Cuts at: 2797 3175 2797
Size: 378 5110
EcoRV GAT'ATC
Cuts at: 2353 2353
Size: 5488
FspI TGC'GCA
Cuts at: 3994 3994
Size: 5488
HaeII r_GCGC'y
Cuts at: 1678 2345 2511 4635 1678
Size: 667 166 2124 2531
Fragments arranged by size:
2531 2124 667 166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2142 2174 4129 4161 4203
Size: 32 135 32 570 32 1955 32 42
Cuts at: 4203 4235 1373
Size: 32 2626
Fragments arranged by size:
2626 1955 570 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2832 3078 669
Size: 988 281 894 246 3079
Fragments arranged by size:
3079 988 894 281 246
HindIII A'AGCT_T
Cuts at: 748 2165 2361 748
Size: 1417 196 3875
Fragments arranged by size:
3875 1417 196
HpaI GTT'AAC
Cuts at: 3078 3078
Size: 5488
KpnI G_GTAC'C
Cuts at: 2813 2813
Size: 5488
MfeI C'AATT_G
Cuts at: 3087 3087
Size: 5488
MluI A'CGCG_T
Cuts at: 5364 5364
Size: 5488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4602 4786 1305
Size: 3297 184 2007
Fragments arranged by size:
3297 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3666
Fragments arranged by size:
3666 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3446 3805 3964 518
Size: 648 2280 359 159 2042
Fragments arranged by size:
2280 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2516 2904 3581 4729 4974 1606
Size: 910 388 677 1148 245 2120
Fragments arranged by size:
2120 1148 910 677 388 245
NcoI C'CATG_G
Cuts at: 513 1065 2076 2604 513
Size: 552 1011 528 3397
Fragments arranged by size:
3397 1011 552 528
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5488
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5488
NotI GC'GGCC_GC
Cuts at: 2935 2935
Size: 5488
NsiI A_TGCA'T
Cuts at: 2495 2495
Size: 5488
NspI r_CATG'y
Cuts at: 1132 1229 2096 2493 2846 4391 1132
Size: 97 867 397 353 1545 2229
Fragments arranged by size:
2229 1545 867 397 353 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5488
PciI A'CATG_T
Cuts at: 4387 4387
Size: 5488
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5488
PfoI T'CCnGG_A
Cuts at: 2024 2024
Size: 5488
PmeI GTTT'AAAC
Cuts at: 2792 2792
Size: 5488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2262 2294 3557 3589 5070 5102 2262
Size: 32 1263 32 1481 32 2648
Fragments arranged by size:
2648 1481 1263 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5488
PsiI TTA'TAA
Cuts at: 2202 3058 2202
Size: 856 4632
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2667 2699 2667
Size: 32 5456
PstI C_TGCA'G
Cuts at: 830 2515 2840 830
Size: 1685 325 3478
Fragments arranged by size:
3478 1685 325
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5488
PvuII CAG'CTG
Cuts at: 2516 2516
Size: 5488
SalI G'TCGA_C
Cuts at: 1936 2830 1936
Size: 894 4594
SbfI CC_TGCA'GG
Cuts at: 2840 2840
Size: 5488
ScaI AGT'ACT
Cuts at: 2592 3736 5448 2592
Size: 1144 1712 2632
Fragments arranged by size:
2632 1712 1144
SfcI C'TryA_G
Cuts at: 826 1045 2511 2836 3971 4652 4843 826
Size: 219 1466 325 1135 681 191 1471
Fragments arranged by size:
1471 1466 1135 681 325 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5488
SmaI CCC'GGG
Cuts at: 1872 2815 1872
Size: 943 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3592 4493 4755 820
Size: 197 926 1649 901 262 1553
Fragments arranged by size:
1649 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3279 493
Size: 2786 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5488
SphI G_CATG'C
Cuts at: 1132 2846 1132
Size: 1714 3774
SspI AAT'ATT
Cuts at: 5 52 3412 5
Size: 47 3360 2081
Fragments arranged by size:
3360 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5488
StyI C'CwwG_G
Cuts at: 513 1065 2076 2604 2927 513
Size: 552 1011 528 323 3074
Fragments arranged by size:
3074 1011 552 528 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3515 3532 3685 3870 4315 325
Size: 1255 1935 17 153 185 445 1498
Fragments arranged by size:
1935 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2409 2590 3734
Size: 274 80 33 51 1443 432 181 1144
Cuts at: 3734 5446 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1144 432 274 181 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5448 5480 5448
Size: 32 5456
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5488
XbaI T'CTAG_A
Cuts at: 2824 2824
Size: 5488
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5488
XmaI C'CCGG_G
Cuts at: 1870 2813 1870
Size: 943 4545
XmnI GAAnn'nnTTC
Cuts at: 3617 3617
Size: 5488
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3296 276
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI