Restriction map for
pFN21AB6320
(Circular) (Six-base) MAPSORT of: pFN21AB6320.seq Check: 2959 from: 1 to: 5551
pFN21AB6320 5551 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3361 278
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2894 1937
Size: 957 4594
Acc65I G'GTAC_C
Cuts at: 2722 2872 2722
Size: 150 5401
AclI AA'CG_TT
Cuts at: 1563 3678 4051 1563
Size: 2115 373 3063
Fragments arranged by size:
3063 2115 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2171 3614 4998 5266 1632
Size: 539 1443 1384 268 1917
Fragments arranged by size:
1917 1443 1384 539 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5354
AflIII A'CryG_T
Cuts at: 1203 4450 5427 1203
Size: 3247 977 1327
Fragments arranged by size:
3247 1327 977
AgeI A'CCGG_T
Cuts at: 4382 4382
Size: 5551
AhdI GACnn_n'nnGTC
Cuts at: 4280 4280
Size: 5551
AleI CACnn'nnGTG
Cuts at: 1166 2306 2399 1166
Size: 1140 93 4318
Fragments arranged by size:
4318 1140 93
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5551
AlwNI CAG_nnn'CTG
Cuts at: 2125 3294 4866 2125
Size: 1169 1572 2810
Fragments arranged by size:
2810 1572 1169
ApaLI G'TGCA_C
Cuts at: 3608 4764 3608
Size: 1156 4395
ApoI r'AATT_y
Cuts at: 1493 2860 3086 3238 5347 1493
Size: 1367 226 152 2109 1697
Fragments arranged by size:
2109 1697 1367 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4105 160
Size: 872 3073 1606
Fragments arranged by size:
3073 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2428 2726 2876 1870
Size: 73 72 413 298 150 4545
Fragments arranged by size:
4545 413 298 150 73 72
BamHI G'GATC_C
Cuts at: 2881 3248 2881
Size: 367 5184
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2300 2452 2722 2872 4327
Size: 325 217 627 513 152 270 150 1455
Cuts at: 4327 618
Size: 1842
Fragments arranged by size:
1842 1455 627 513 325 270 217 152
150
BanII G_rGCy'C
Cuts at: 1391 2670 2870 1391
Size: 1279 200 4072
Fragments arranged by size:
4072 1279 200
BbeI G_GCGC'C
Cuts at: 2456 2456
Size: 5551
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4580
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3742 3776 1903
Size: 34 1805 34 3678
Fragments arranged by size:
3678 1805 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2264 3443 4653 2264
Size: 1179 1210 3162
Fragments arranged by size:
3162 1210 1179
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5551
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4162 136
Size: 107 122 71 631 605 2490 1525
Fragments arranged by size:
2490 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2832 5546 2832
Size: 2714 2837
BlpI GC'TnA_GC
Cuts at: 2968 2968
Size: 5551
Bme1580I G_kGCm'C
Cuts at: 1790 3612 4768 1790
Size: 1822 1156 2573
Fragments arranged by size:
2573 1822 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5551
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2104 3282 4240
Size: 416 53 83 399 24 687 1178 958
Cuts at: 4240 442
Size: 1753
Fragments arranged by size:
1753 1178 958 687 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4211 1239
Size: 2972 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3676 4541 4839 3676
Size: 865 298 4388
Fragments arranged by size:
4388 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2012 2348 2651 4214 882
Size: 617 513 336 303 1563 2219
Fragments arranged by size:
2219 1563 617 513 336 303
BsaAI yAC'GTr
Cuts at: 493 1204 3342 493
Size: 711 2138 2702
Fragments arranged by size:
2702 2138 711
BsaBI GATnn'nnATC
Cuts at: 5545 5545
Size: 5551
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2453 3358 3740 4375
Size: 53 83 186 936 920 905 382 635
Cuts at: 4375 275
Size: 1451
Fragments arranged by size:
1451 936 920 905 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3984 4382 4656 4803 5214 3984
Size: 398 274 147 411 4321
Fragments arranged by size:
4321 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5326
Fragments arranged by size:
5326 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2669 1186
Size: 1483 4068
BseYI C'CCAG_C
Cuts at: 2598 4754 5250 2598
Size: 2156 496 2899
Fragments arranged by size:
2899 2156 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4978
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3001 3762 4790 1540
Size: 275 186 1000 761 1028 2301
Fragments arranged by size:
2301 1028 1000 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2670 3612 3697 4768 2670
Size: 942 85 1071 3453
Fragments arranged by size:
3453 1071 942 85
BsmI GAATG_Cn'
Cuts at: 3062 3155 3062
Size: 93 5458
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2670 2870 3612 3697 4768 1391
Size: 399 880 200 742 85 1071 2174
Fragments arranged by size:
2174 1071 880 742 399 200 85
BspEI T'CCGG_A
Cuts at: 5214 5214
Size: 5551
BspHI T'CATG_A
Cuts at: 3440 5170 3440
Size: 1730 3821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2655 2906 844
Size: 1811 251 3489
Fragments arranged by size:
3489 1811 251
BsrBI CCG'CTC
Cuts at: 1394 1823 2314 3438 1394
Size: 429 491 1124 3507
Fragments arranged by size:
3507 1124 491 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2283 4046 4220 65
Size: 1183 1035 1763 174 1396
Fragments arranged by size:
1763 1396 1183 1035 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4195 4382 1700
Size: 110 2385 187 2869
Fragments arranged by size:
2869 2385 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5551
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5551
BssSI C'ACGA_G
Cuts at: 3611 4623 3611
Size: 1012 4539
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2473 2575 794
Size: 1679 102 3770
Fragments arranged by size:
3770 1679 102
BstBI TT'CG_AA
Cuts at: 4355 4355
Size: 5551
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5551
BstXI CCAn_nnnn'nTGG
Cuts at: 2401 2401
Size: 5551
BstYI r'GATC_y
Cuts at: 1972 2121 2541 2832 2881 3248 3633 3650 5102
Size: 149 420 291 49 367 385 17 1452
Cuts at: 5102 5188 5200 5546 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 420 385 367 346 291 149
86 49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2400 513
Size: 552 939 396 3664
Fragments arranged by size:
3664 939 552 396
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2725 2802 487
Size: 683 56 1499 77 3236
Fragments arranged by size:
3236 1499 683 77 56
BtsI GCAGTG_nn'
Cuts at: 3067 3861 3881 3067
Size: 794 20 4737
Fragments arranged by size:
4737 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5551
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5516
DraI TTT'AAA
Cuts at: 2855 2855
Size: 5551
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4558 809
Size: 507 3242 1802
Fragments arranged by size:
3242 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2116 2361 2536
Size: 54 1425 50 275 18 286 245 175
Cuts at: 2536 2998 3887 5516 8
Size: 462 889 1629 43
Fragments arranged by size:
1629 1425 889 462 286 275 245 175
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2998 1537
Size: 275 1186 4090
Fragments arranged by size:
4090 1186 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2327 3481 1353
Size: 974 1154 3423
Fragments arranged by size:
3423 1154 974
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4138 4512 4658 387
Size: 1064 138 2549 374 146 1280
Fragments arranged by size:
2549 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2668 2668
Size: 5551
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 3614 4211 4998 5266 1239
Size: 393 539 1443 597 787 268 1524
Fragments arranged by size:
1524 1443 787 597 539 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2651 2651
Size: 5551
EcoO109I rG'GnC_Cy
Cuts at: 2420 2420
Size: 5551
EcoRI G'AATT_C
Cuts at: 2860 3238 2860
Size: 378 5173
EcoRV GAT'ATC
Cuts at: 2131 2281 2131
Size: 150 5401
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2854 2854
Size: 5551
FspI TGC'GCA
Cuts at: 4057 4057
Size: 5551
HaeII r_GCGC'y
Cuts at: 1678 2199 2456 4698 1678
Size: 521 257 2242 2531
Fragments arranged by size:
2531 2242 521 257
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4192 4224 4266 4298 1373
Size: 32 135 32 2620 32 42 32 2626
Fragments arranged by size:
2626 2620 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2895 3141 669
Size: 988 281 957 246 3079
Fragments arranged by size:
3079 988 957 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5551
HpaI GTT'AAC
Cuts at: 3141 3141
Size: 5551
KasI G'GCGC_C
Cuts at: 2452 2452
Size: 5551
KpnI G_GTAC'C
Cuts at: 2726 2876 2726
Size: 150 5401
MfeI C'AATT_G
Cuts at: 3150 3150
Size: 5551
MluI A'CGCG_T
Cuts at: 5427 5427
Size: 5551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4665 4849 1305
Size: 3360 184 2007
Fragments arranged by size:
3360 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2118 2538 10
Size: 54 1425 343 286 420 3023
Fragments arranged by size:
3023 1425 420 343 286 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2306 2399 3509 3868 4027 518
Size: 648 1140 93 1110 359 159 2042
Fragments arranged by size:
2042 1140 1110 648 359 159 93
MspA1I CmG'CkG
Cuts at: 1606 2967 3644 4792 5037 1606
Size: 1361 677 1148 245 2120
Fragments arranged by size:
2120 1361 1148 677 245
NarI GG'CG_CC
Cuts at: 2453 2453
Size: 5551
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4060
Fragments arranged by size:
4060 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5551
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5551
NotI GC'GGCC_GC
Cuts at: 2998 2998
Size: 5551
NruI TCG'CGA
Cuts at: 2787 2787
Size: 5551
NspI r_CATG'y
Cuts at: 1132 1229 2437 2476 2497 2909 4454 1132
Size: 97 1208 39 21 412 1545 2229
Fragments arranged by size:
2229 1545 1208 412 97 39 21
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5551
PciI A'CATG_T
Cuts at: 4450 4450
Size: 5551
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2125 1108
Size: 1017 4534
PfoI T'CCnGG_A
Cuts at: 2124 2124
Size: 5551
PmeI GTTT'AAAC
Cuts at: 2855 2855
Size: 5551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3620 3652 5133 5165 3620
Size: 32 1481 32 4006
Fragments arranged by size:
4006 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5551
PsiI TTA'TAA
Cuts at: 3121 3121
Size: 5551
PstI C_TGCA'G
Cuts at: 830 2799 2903 830
Size: 1969 104 3478
Fragments arranged by size:
3478 1969 104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5551
SacI G_AGCT'C
Cuts at: 2670 2670
Size: 5551
SalI G'TCGA_C
Cuts at: 1936 2893 1936
Size: 957 4594
SbfI CC_TGCA'GG
Cuts at: 2799 2903 2799
Size: 104 5447
ScaI AGT'ACT
Cuts at: 3799 5511 3799
Size: 1712 3839
SfcI C'TryA_G
Cuts at: 826 1045 2795 2899 4034 4715 4906 826
Size: 219 1750 104 1135 681 191 1471
Fragments arranged by size:
1750 1471 1135 681 219 191 104
SfoI GGC'GCC
Cuts at: 2454 2454
Size: 5551
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5551
SmaI CCC'GGG
Cuts at: 1872 2430 2728 2878 1872
Size: 558 298 150 4545
Fragments arranged by size:
4545 558 298 150
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3655 4556 4818 820
Size: 197 926 1712 901 262 1553
Fragments arranged by size:
1712 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3342 493
Size: 2849 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5551
SphI G_CATG'C
Cuts at: 1132 2437 2909 1132
Size: 1305 472 3774
Fragments arranged by size:
3774 1305 472
SspI AAT'ATT
Cuts at: 5 52 3475 5
Size: 47 3423 2081
Fragments arranged by size:
3423 2081 47
StuI AGG'CCT
Cuts at: 1517 2168 2526 1517
Size: 651 358 4542
Fragments arranged by size:
4542 651 358
StyI C'CwwG_G
Cuts at: 513 1065 2004 2990 513
Size: 552 939 986 3074
Fragments arranged by size:
3074 986 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2285 3578 3595 3748 3933 4378 325
Size: 1255 705 1293 17 153 185 445 1498
Fragments arranged by size:
1498 1293 1255 705 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3797 5509 96
Size: 274 80 33 51 1443 1820 1712 138
Fragments arranged by size:
1820 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5511 5543 5511
Size: 32 5519
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5551
XbaI T'CTAG_A
Cuts at: 2887 2887
Size: 5551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2401 2401
Size: 5551
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5551
XmaI C'CCGG_G
Cuts at: 1870 2428 2726 2876 1870
Size: 558 298 150 4545
Fragments arranged by size:
4545 558 298 150
XmnI GAAnn'nnTTC
Cuts at: 3680 3680
Size: 5551
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3359 276
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SacI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
FseI FspAI NaeI NgoMIV NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SrfI SwaI