Restriction map for
pFN21AB6319
(Circular) (Six-base) MAPSORT of: pFN21AB6319.seq Check: 7709 from: 1 to: 5485
pFN21AB6319 5485 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2200 2200
Size: 5485
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3295 278
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2828 1937
Size: 891 4594
Acc65I G'GTAC_C
Cuts at: 2806 2806
Size: 5485
AclI AA'CG_TT
Cuts at: 1563 3612 3985 1563
Size: 2049 373 3063
Fragments arranged by size:
3063 2049 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3548 4932 5200 1632
Size: 1916 1384 268 1917
Fragments arranged by size:
1917 1916 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5288
AflIII A'CryG_T
Cuts at: 1203 4384 5361 1203
Size: 3181 977 1327
Fragments arranged by size:
3181 1327 977
AgeI A'CCGG_T
Cuts at: 4316 4316
Size: 5485
AhdI GACnn_n'nnGTC
Cuts at: 4214 4214
Size: 5485
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2404 1184
Size: 1220 4265
AlwNI CAG_nnn'CTG
Cuts at: 2501 3228 4800 2501
Size: 727 1572 3186
Fragments arranged by size:
3186 1572 727
ApaI G_GGCC'C
Cuts at: 2198 2198
Size: 5485
ApaLI G'TGCA_C
Cuts at: 3542 4698 3542
Size: 1156 4329
ApoI r'AATT_y
Cuts at: 1493 2405 2794 3020 3172 5281 1493
Size: 912 389 226 152 2109 1697
Fragments arranged by size:
2109 1697 912 389 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4039 160
Size: 872 3007 1606
Fragments arranged by size:
3007 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
AvaI C'yCGr_G
Cuts at: 1870 1943 2031 2810 1870
Size: 73 88 779 4545
Fragments arranged by size:
4545 779 88 73
BamHI G'GATC_C
Cuts at: 2815 3182 2815
Size: 367 5118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2174 2806 4261 618
Size: 325 217 627 387 632 1455 1842
Fragments arranged by size:
1842 1455 632 627 387 325 217
BanII G_rGCy'C
Cuts at: 1391 2198 2804 1391
Size: 807 606 4072
Fragments arranged by size:
4072 807 606
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4514
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3676 3710 1903
Size: 34 1739 34 3678
Fragments arranged by size:
3678 1739 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3377 4587 3377
Size: 1210 4275
BclI T'GATC_A
Cuts at: 1548 2653 1548
Size: 1105 4380
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4096 136
Size: 107 122 71 631 605 2424 1525
Fragments arranged by size:
2424 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5480 5480
Size: 5485
BlpI GC'TnA_GC
Cuts at: 2902 2902
Size: 5485
Bme1580I G_kGCm'C
Cuts at: 1790 2198 3546 4702 1790
Size: 408 1348 1156 2573
Fragments arranged by size:
2573 1348 1156 408
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5485
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2473 3216 4174
Size: 416 53 83 399 24 1056 743 958
Cuts at: 4174 442
Size: 1753
Fragments arranged by size:
1753 1056 958 743 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 2478 4145 1239
Size: 1046 193 1667 2579
Fragments arranged by size:
2579 1667 1046 193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2547 2659 3610 4475 4773 2547
Size: 112 951 865 298 3259
Fragments arranged by size:
3259 951 865 298 112
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4148 882
Size: 617 2649 2219
Fragments arranged by size:
2649 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3276 493
Size: 711 2072 2702
Fragments arranged by size:
2702 2072 711
BsaBI GATnn'nnATC
Cuts at: 5479 5479
Size: 5485
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3292 3674 4309 275
Size: 53 83 186 936 1759 382 635 1451
Fragments arranged by size:
1759 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2064 3918 4316 4590 4737 5148 2064
Size: 1854 398 274 147 411 2401
Fragments arranged by size:
2401 1854 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2111 2141 1178
Size: 30 165 30 708 30 4522
Fragments arranged by size:
4522 708 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2119 1186
Size: 933 4552
BseYI C'CCAG_C
Cuts at: 4688 5184 4688
Size: 496 4989
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2336 1163
Size: 573 600 4312
Fragments arranged by size:
4312 600 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2935 3696 4724 1540
Size: 275 186 934 761 1028 2301
Fragments arranged by size:
2301 1028 934 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3546 3631 4702 3546
Size: 85 1071 4329
Fragments arranged by size:
4329 1071 85
BsmI GAATG_Cn'
Cuts at: 2551 2996 3089 2551
Size: 445 93 4947
Fragments arranged by size:
4947 445 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2093 2093
Size: 5485
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2198 2804 3546 3631 4702 1391
Size: 399 408 606 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 606 408 399 85
BspEI T'CCGG_A
Cuts at: 5148 5148
Size: 5485
BspHI T'CATG_A
Cuts at: 3374 5104 3374
Size: 1730 3755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2200 2840 844
Size: 1356 640 3489
Fragments arranged by size:
3489 1356 640
BsrBI CCG'CTC
Cuts at: 1394 1823 3372 1394
Size: 429 1549 3507
Fragments arranged by size:
3507 1549 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3980 4154 65
Size: 1183 2732 174 1396
Fragments arranged by size:
2732 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4129 4316 1700
Size: 110 2319 187 2869
Fragments arranged by size:
2869 2319 187 110
BsrGI T'GTAC_A
Cuts at: 96 2689 96
Size: 2593 2892
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5485
BssSI C'ACGA_G
Cuts at: 3545 4557 3545
Size: 1012 4473
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2314 794
Size: 1520 3965
BstBI TT'CG_AA
Cuts at: 4289 4289
Size: 5485
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5485
BstYI r'GATC_y
Cuts at: 1972 2815 3182 3567 3584 5036 5122 5134 5480
Size: 843 367 385 17 1452 86 12 346
Cuts at: 5480 1972
Size: 1977
Fragments arranged by size:
1977 1452 843 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2089 2593 2089
Size: 504 4981
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4933
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4746
Fragments arranged by size:
4746 683 56
BtsI GCAGTG_nn'
Cuts at: 3001 3795 3815 3001
Size: 794 20 4671
Fragments arranged by size:
4671 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5485
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5450
DraI TTT'AAA
Cuts at: 2789 2789
Size: 5485
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4492 809
Size: 507 3176 1802
Fragments arranged by size:
3176 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2584 2932 3821
Size: 54 1425 50 275 18 754 348 889
Cuts at: 3821 5450 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 754 348 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2932 1537
Size: 275 1120 4090
Fragments arranged by size:
4090 1120 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2762 3415 1353
Size: 1409 653 3423
Fragments arranged by size:
3423 1409 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4072 4446 4592 387
Size: 1064 138 2483 374 146 1280
Fragments arranged by size:
2483 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2478 3548 4145 4932 5200 1239
Size: 393 653 193 1070 597 787 268 1524
Fragments arranged by size:
1524 1070 787 653 597 393 268 193
EcoO109I rG'GnC_Cy
Cuts at: 2195 2195
Size: 5485
EcoRI G'AATT_C
Cuts at: 2794 3172 2794
Size: 378 5107
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2546 2546
Size: 5485
FspI TGC'GCA
Cuts at: 3991 3991
Size: 5485
HaeII r_GCGC'y
Cuts at: 1678 4632 1678
Size: 2954 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2254 2286 2645 2677 4126
Size: 32 135 32 682 32 359 32 1449
Cuts at: 4126 4158 4200 4232 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1449 682 359 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2829 3075 669
Size: 988 281 891 246 3079
Fragments arranged by size:
3079 988 891 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5485
HpaI GTT'AAC
Cuts at: 3075 3075
Size: 5485
KpnI G_GTAC'C
Cuts at: 2810 2810
Size: 5485
MfeI C'AATT_G
Cuts at: 3084 3084
Size: 5485
MluI A'CGCG_T
Cuts at: 5361 5361
Size: 5485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2051 4599 4783 1305
Size: 746 2548 184 2007
Fragments arranged by size:
2548 2007 746 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2586 10
Size: 54 1425 343 754 2909
Fragments arranged by size:
2909 1425 754 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3443 3802 3961 518
Size: 648 2277 359 159 2042
Fragments arranged by size:
2277 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2901 3578 4726 4971 1606
Size: 1295 677 1148 245 2120
Fragments arranged by size:
2120 1295 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4933
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5485
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5485
NotI GC'GGCC_GC
Cuts at: 2932 2932
Size: 5485
NspI r_CATG'y
Cuts at: 1132 1229 2723 2843 4388 1132
Size: 97 1494 120 1545 2229
Fragments arranged by size:
2229 1545 1494 120 97
PasI CC'CwG_GG
Cuts at: 1348 2055 1348
Size: 707 4778
PciI A'CATG_T
Cuts at: 4384 4384
Size: 5485
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5485
PmeI GTTT'AAAC
Cuts at: 2789 2789
Size: 5485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 5067 5099 3554
Size: 32 1481 32 3940
Fragments arranged by size:
3940 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5485
PsiI TTA'TAA
Cuts at: 2741 3055 2741
Size: 314 5171
PspOMI G'GGCC_C
Cuts at: 2194 2194
Size: 5485
PstI C_TGCA'G
Cuts at: 830 2837 830
Size: 2007 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5485
SalI G'TCGA_C
Cuts at: 1936 2827 1936
Size: 891 4594
SbfI CC_TGCA'GG
Cuts at: 2837 2837
Size: 5485
ScaI AGT'ACT
Cuts at: 3733 5445 3733
Size: 1712 3773
SfcI C'TryA_G
Cuts at: 826 1045 2833 3968 4649 4840 826
Size: 219 1788 1135 681 191 1471
Fragments arranged by size:
1788 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5485
SmaI CCC'GGG
Cuts at: 1872 2812 1872
Size: 940 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2562 2674 3589 4490 4752 820
Size: 197 926 619 112 915 901 262 1553
Fragments arranged by size:
1553 926 915 901 619 262 197 112
SnaBI TAC'GTA
Cuts at: 493 3276 493
Size: 2783 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5485
SphI G_CATG'C
Cuts at: 1132 2843 1132
Size: 1711 3774
SspI AAT'ATT
Cuts at: 5 52 2425 3409 5
Size: 47 2373 984 2081
Fragments arranged by size:
2373 2081 984 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5485
StyI C'CwwG_G
Cuts at: 513 1065 2235 2924 513
Size: 552 1170 689 3074
Fragments arranged by size:
3074 1170 689 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3512 3529 3682 3867 4312 325
Size: 1255 1932 17 153 185 445 1498
Fragments arranged by size:
1932 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2689 3731 5443
Size: 274 80 33 51 1443 712 1042 1712
Cuts at: 5443 96
Size: 138
Fragments arranged by size:
1712 1443 1042 712 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2111 2143 2648 2680 5445 5477 2111
Size: 32 505 32 2765 32 2119
Fragments arranged by size:
2765 2119 505 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5485
XbaI T'CTAG_A
Cuts at: 2278 2821 2278
Size: 543 4942
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5485
XmaI C'CCGG_G
Cuts at: 1870 2810 1870
Size: 940 4545
XmnI GAAnn'nnTTC
Cuts at: 2049 3614 2049
Size: 1565 3920
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3293 276
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI