Restriction map for
pFN21AB6312
(Circular) (Six-base) MAPSORT of: pFN21AB6312.seq Check: 3511 from: 1 to: 5545
pFN21AB6312 5545 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3355 278
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2888 1937
Size: 951 4594
Acc65I G'GTAC_C
Cuts at: 2866 2866
Size: 5545
AclI AA'CG_TT
Cuts at: 1563 3672 4045 1563
Size: 2109 373 3063
Fragments arranged by size:
3063 2109 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3608 4992 5260 1632
Size: 1976 1384 268 1917
Fragments arranged by size:
1976 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5348
AflIII A'CryG_T
Cuts at: 1203 4444 5421 1203
Size: 3241 977 1327
Fragments arranged by size:
3241 1327 977
AgeI A'CCGG_T
Cuts at: 4376 4376
Size: 5545
AhdI GACnn_n'nnGTC
Cuts at: 4274 4274
Size: 5545
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5545
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2772 1184
Size: 1588 3957
AlwNI CAG_nnn'CTG
Cuts at: 2251 3288 4860 2251
Size: 1037 1572 2936
Fragments arranged by size:
2936 1572 1037
ApaLI G'TGCA_C
Cuts at: 3602 4758 3602
Size: 1156 4389
ApoI r'AATT_y
Cuts at: 1493 2854 3080 3232 5341 1493
Size: 1361 226 152 2109 1697
Fragments arranged by size:
2109 1697 1361 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4099 160
Size: 872 3067 1606
Fragments arranged by size:
3067 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
AvaI C'yCGr_G
Cuts at: 1870 1943 2870 1870
Size: 73 927 4545
Fragments arranged by size:
4545 927 73
AvrII C'CTAG_G
Cuts at: 2012 2012
Size: 5545
BamHI G'GATC_C
Cuts at: 2767 2875 3242 2767
Size: 108 367 5070
Fragments arranged by size:
5070 367 108
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2866 4321 618
Size: 325 217 627 1079 1455 1842
Fragments arranged by size:
1842 1455 1079 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2864 1391
Size: 1473 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2584 928
Size: 971 685 3889
Fragments arranged by size:
3889 971 685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3736 3770 1903
Size: 34 1799 34 3678
Fragments arranged by size:
3678 1799 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3437 4647 3437
Size: 1210 4335
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5545
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4156 136
Size: 107 122 71 631 605 2484 1525
Fragments arranged by size:
2484 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2682 5540 2682
Size: 2858 2687
BlpI GC'TnA_GC
Cuts at: 2962 2962
Size: 5545
Bme1580I G_kGCm'C
Cuts at: 1790 3606 4762 1790
Size: 1816 1156 2573
Fragments arranged by size:
2573 1816 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5545
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3276 4234 442
Size: 416 53 83 399 24 1859 958 1753
Fragments arranged by size:
1859 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2544 2601 4205 1239
Size: 1305 57 1604 2579
Fragments arranged by size:
2579 1604 1305 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2129 2569 3670 4535 4833 2129
Size: 440 1101 865 298 2841
Fragments arranged by size:
2841 1101 865 440 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4208 882
Size: 617 2709 2219
Fragments arranged by size:
2709 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3336 493
Size: 711 2132 2702
Fragments arranged by size:
2702 2132 711
BsaBI GATnn'nnATC
Cuts at: 5539 5539
Size: 5545
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3352 3734 4369 275
Size: 53 83 186 936 1819 382 635 1451
Fragments arranged by size:
1819 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3978 4376 4650 4797 5208 3978
Size: 398 274 147 411 4315
Fragments arranged by size:
4315 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5320
Fragments arranged by size:
5320 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 1186
Size: 946 4599
BseYI C'CCAG_C
Cuts at: 2518 2760 4748 5244 2518
Size: 242 1988 496 2819
Fragments arranged by size:
2819 1988 496 242
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4972
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2092 2995 3756 4784 1540
Size: 275 186 91 903 761 1028 2301
Fragments arranged by size:
2301 1028 903 761 275 186 91
BsiHKAI G_wGCw'C
Cuts at: 3606 3691 4762 3606
Size: 85 1071 4389
Fragments arranged by size:
4389 1071 85
BsmI GAATG_Cn'
Cuts at: 3056 3149 3056
Size: 93 5452
BsmBI CGTCTCn'nnnn_
Cuts at: 2813 2813
Size: 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2864 3606 3691 4762 1391
Size: 399 1074 742 85 1071 2174
Fragments arranged by size:
2174 1074 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5208 5208
Size: 5545
BspHI T'CATG_A
Cuts at: 3434 5164 3434
Size: 1730 3815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2900 844
Size: 2056 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2632 3432 1394
Size: 429 809 800 3507
Fragments arranged by size:
3507 809 800 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4040 4214 65
Size: 1183 2792 174 1396
Fragments arranged by size:
2792 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4189 4376 1700
Size: 110 2379 187 2869
Fragments arranged by size:
2869 2379 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5545
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5545
BssSI C'ACGA_G
Cuts at: 3605 4617 3605
Size: 1012 4533
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5545
BstBI TT'CG_AA
Cuts at: 4349 4349
Size: 5545
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5545
BstYI r'GATC_y
Cuts at: 1972 2175 2682 2767 2875 3242 3627 3644 5096
Size: 203 507 85 108 367 385 17 1452
Cuts at: 5096 5182 5194 5540 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 507 385 367 346 203 108
86 85 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2523 2544 513
Size: 552 939 519 21 3514
Fragments arranged by size:
3514 939 552 519 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4806
Fragments arranged by size:
4806 683 56
BtsI GCAGTG_nn'
Cuts at: 3061 3855 3875 3061
Size: 794 20 4731
Fragments arranged by size:
4731 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5545
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5510
DraI TTT'AAA
Cuts at: 2849 2849
Size: 5545
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2588 4552 809
Size: 507 1272 1964 1802
Fragments arranged by size:
1964 1802 1272 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2730 2992
Size: 54 1425 50 275 18 204 696 262
Cuts at: 2992 3881 5510 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 696 275 262 204 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2992 1537
Size: 275 1180 4090
Fragments arranged by size:
4090 1180 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2250 2373 2447 3475 1353
Size: 897 123 74 1028 3423
Fragments arranged by size:
3423 1028 897 123 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2074 2724 4132 4506 4652 387
Size: 1064 138 485 650 1408 374 146 1280
Fragments arranged by size:
1408 1280 1064 650 485 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2544 2601 3608 4205 4992 5260 1239
Size: 393 912 57 1007 597 787 268 1524
Fragments arranged by size:
1524 1007 912 787 597 393 268 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2053 2144 2147 2255 2053
Size: 91 3 108 5343
Fragments arranged by size:
5343 108 91 3
EcoO109I rG'GnC_Cy
Cuts at: 2248 2248
Size: 5545
EcoRI G'AATT_C
Cuts at: 2854 3232 2854
Size: 378 5167
EcoRV GAT'ATC
Cuts at: 2281 2281
Size: 5545
FspI TGC'GCA
Cuts at: 4051 4051
Size: 5545
HaeII r_GCGC'y
Cuts at: 1678 4692 1678
Size: 3014 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4186 4218 4260 4292 1373
Size: 32 135 32 2614 32 42 32 2626
Fragments arranged by size:
2626 2614 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2889 3135 669
Size: 988 281 951 246 3079
Fragments arranged by size:
3079 988 951 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5545
HpaI GTT'AAC
Cuts at: 3135 3135
Size: 5545
KpnI G_GTAC'C
Cuts at: 2870 2870
Size: 5545
MfeI C'AATT_G
Cuts at: 3144 3144
Size: 5545
MluI A'CGCG_T
Cuts at: 5421 5421
Size: 5545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2826 4659 4843 1305
Size: 1521 1833 184 2007
Fragments arranged by size:
2007 1833 1521 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2036 2732 10
Size: 54 1425 343 204 696 2823
Fragments arranged by size:
2823 1425 696 343 204 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3503 3862 4021 518
Size: 648 2337 359 159 2042
Fragments arranged by size:
2337 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2270 2764 2961 3638 4786 5031 1606
Size: 664 494 197 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 664 494 245 197
NcoI C'CATG_G
Cuts at: 513 1065 2004 2544 513
Size: 552 939 540 3514
Fragments arranged by size:
3514 939 552 540
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5545
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5545
NotI GC'GGCC_GC
Cuts at: 2992 2992
Size: 5545
NspI r_CATG'y
Cuts at: 1132 1229 2903 4448 1132
Size: 97 1674 1545 2229
Fragments arranged by size:
2229 1674 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5545
PciI A'CATG_T
Cuts at: 4444 4444
Size: 5545
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5545
PmeI GTTT'AAAC
Cuts at: 2849 2849
Size: 5545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3614 3646 5127 5159 3614
Size: 32 1481 32 4000
Fragments arranged by size:
4000 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5545
PsiI TTA'TAA
Cuts at: 3115 3115
Size: 5545
PstI C_TGCA'G
Cuts at: 830 2825 2897 830
Size: 1995 72 3478
Fragments arranged by size:
3478 1995 72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5545
PvuII CAG'CTG
Cuts at: 2270 2764 2270
Size: 494 5051
SalI G'TCGA_C
Cuts at: 1936 2887 1936
Size: 951 4594
SbfI CC_TGCA'GG
Cuts at: 2897 2897
Size: 5545
ScaI AGT'ACT
Cuts at: 3793 5505 3793
Size: 1712 3833
SfcI C'TryA_G
Cuts at: 826 1045 2821 2893 4028 4709 4900 826
Size: 219 1776 72 1135 681 191 1471
Fragments arranged by size:
1776 1471 1135 681 219 191 72
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5545
SmaI CCC'GGG
Cuts at: 1872 2872 1872
Size: 1000 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2144 2584 3649 4550 4812 820
Size: 197 926 201 440 1065 901 262 1553
Fragments arranged by size:
1553 1065 926 901 440 262 201 197
SnaBI TAC'GTA
Cuts at: 493 3336 493
Size: 2843 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5545
SphI G_CATG'C
Cuts at: 1132 2903 1132
Size: 1771 3774
SspI AAT'ATT
Cuts at: 5 52 3469 5
Size: 47 3417 2081
Fragments arranged by size:
3417 2081 47
StuI AGG'CCT
Cuts at: 1517 2565 2777 1517
Size: 1048 212 4285
Fragments arranged by size:
4285 1048 212
StyI C'CwwG_G
Cuts at: 513 1065 2004 2012 2190 2230 2544 2778 2984
Size: 552 939 8 178 40 314 234 206
Cuts at: 2984 513
Size: 3074
Fragments arranged by size:
3074 939 552 314 234 206 178 40
8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2587 3572 3589 3742 3927 4372 325
Size: 1255 1007 985 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1007 985 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3791 5503 96
Size: 274 80 33 51 1443 1814 1712 138
Fragments arranged by size:
1814 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5505 5537 5505
Size: 32 5513
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5545
XbaI T'CTAG_A
Cuts at: 2881 2881
Size: 5545
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5545
XmaI C'CCGG_G
Cuts at: 1870 2870 1870
Size: 1000 4545
XmnI GAAnn'nnTTC
Cuts at: 2084 2313 3674 2084
Size: 229 1361 3955
Fragments arranged by size:
3955 1361 229
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3353 276
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI