Restriction map for
pFN21AB6310
(Circular) (Six-base) MAPSORT of: pFN21AB6310.seq Check: 654 from: 1 to: 5545
pFN21AB6310 5545 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3355 278
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2888 1937
Size: 951 4594
Acc65I G'GTAC_C
Cuts at: 2866 2866
Size: 5545
AclI AA'CG_TT
Cuts at: 1563 2844 3672 4045 1563
Size: 1281 828 373 3063
Fragments arranged by size:
3063 1281 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2570 2612 3608 4992 5260 1632
Size: 938 42 996 1384 268 1917
Fragments arranged by size:
1917 1384 996 938 268 42
AfeI AGC'GCT
Cuts at: 2497 2497
Size: 5545
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5348
AflIII A'CryG_T
Cuts at: 1203 4444 5421 1203
Size: 3241 977 1327
Fragments arranged by size:
3241 1327 977
AgeI A'CCGG_T
Cuts at: 4376 4376
Size: 5545
AhdI GACnn_n'nnGTC
Cuts at: 2218 4274 2218
Size: 2056 3489
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5545
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5545
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2819 2851 2819
Size: 32 5513
AlwNI CAG_nnn'CTG
Cuts at: 3288 4860 3288
Size: 1572 3973
ApaI G_GGCC'C
Cuts at: 2755 2755
Size: 5545
ApaLI G'TGCA_C
Cuts at: 3602 4758 3602
Size: 1156 4389
ApoI r'AATT_y
Cuts at: 1493 2854 3080 3232 5341 1493
Size: 1361 226 152 2109 1697
Fragments arranged by size:
2109 1697 1361 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4099 160
Size: 872 3067 1606
Fragments arranged by size:
3067 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
AvaI C'yCGr_G
Cuts at: 1870 1943 2098 2322 2531 2747 2762 2782 2870
Size: 73 155 224 209 216 15 20 88
Cuts at: 2870 1870
Size: 4545
Fragments arranged by size:
4545 224 216 209 155 88 73 20
15
BamHI G'GATC_C
Cuts at: 2167 2875 3242 2167
Size: 708 367 4470
Fragments arranged by size:
4470 708 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2866 4321 618
Size: 325 217 627 1079 1455 1842
Fragments arranged by size:
1842 1455 1079 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2685 2755 2789 2864 1391
Size: 621 673 70 34 75 4072
Fragments arranged by size:
4072 673 621 75 70 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4574
BbvCI CC'TCA_GC
Cuts at: 2397 2397
Size: 5545
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2453 2487 3736 3770 1903
Size: 34 516 34 1249 34 3678
Fragments arranged by size:
3678 1249 516 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3437 4647 3437
Size: 1210 4335
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5545
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2372 2547 4156
Size: 107 122 71 631 605 700 175 1609
Cuts at: 4156 136
Size: 1525
Fragments arranged by size:
1609 1525 700 631 605 175 122 107
71
BglII A'GATC_T
Cuts at: 2089 2566 5540 2089
Size: 477 2974 2094
Fragments arranged by size:
2974 2094 477
BlpI GC'TnA_GC
Cuts at: 2962 2962
Size: 5545
Bme1580I G_kGCm'C
Cuts at: 1790 2044 2368 2755 3606 4762 1790
Size: 254 324 387 851 1156 2573
Fragments arranged by size:
2573 1156 851 387 324 254
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5545
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2595 3276 4234
Size: 416 53 83 399 24 1178 681 958
Cuts at: 4234 442
Size: 1753
Fragments arranged by size:
1753 1178 958 681 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2106 2200 2380 2591 4205 1239
Size: 867 94 180 211 1614 2579
Fragments arranged by size:
2579 1614 867 211 180 94
Bpu10I CC'TnA_GC
Cuts at: 2397 2397
Size: 5545
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3670 4535 4833 3670
Size: 865 298 4382
Fragments arranged by size:
4382 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2019 4208 882
Size: 617 520 2189 2219
Fragments arranged by size:
2219 2189 617 520
BsaAI yAC'GTr
Cuts at: 493 1204 3336 493
Size: 711 2132 2702
Fragments arranged by size:
2702 2132 711
BsaBI GATnn'nnATC
Cuts at: 5539 5539
Size: 5545
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3352 3734 4369 275
Size: 53 83 186 936 1819 382 635 1451
Fragments arranged by size:
1819 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3978 4376 4650 4797 5208 3978
Size: 398 274 147 411 4315
Fragments arranged by size:
4315 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2104 2134 2795 2825 1178
Size: 30 165 30 701 30 661 30 3898
Fragments arranged by size:
3898 701 661 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2534 2629 2693 2837 1186
Size: 1348 95 64 144 3894
Fragments arranged by size:
3894 1348 144 95 64
BseYI C'CCAG_C
Cuts at: 2367 2379 2392 2645 2687 2714 4748 5244 2367
Size: 12 13 253 42 27 2034 496 2668
Fragments arranged by size:
2668 2034 496 253 42 27 13 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2300 2510 1163
Size: 573 564 210 4198
Fragments arranged by size:
4198 573 564 210
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2995 3756 4784 1540
Size: 275 186 994 761 1028 2301
Fragments arranged by size:
2301 1028 994 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3606 3691 4762 3606
Size: 85 1071 4389
Fragments arranged by size:
4389 1071 85
BsmI GAATG_Cn'
Cuts at: 3056 3149 3056
Size: 93 5452
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2044 2368 2685 2755 2789 2864
Size: 399 222 32 324 317 70 34 75
Cuts at: 2864 3606 3691 4762 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 324 317 222 85
75 70 34 32
BspEI T'CCGG_A
Cuts at: 5208 5208
Size: 5545
BspHI T'CATG_A
Cuts at: 3434 5164 3434
Size: 1730 3815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2900 844
Size: 2056 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3432 1394
Size: 429 1609 3507
Fragments arranged by size:
3507 1609 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4040 4214 65
Size: 1183 2792 174 1396
Fragments arranged by size:
2792 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4189 4376 1700
Size: 110 2379 187 2869
Fragments arranged by size:
2869 2379 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5545
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5545
BssSI C'ACGA_G
Cuts at: 3605 4617 3605
Size: 1012 4533
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5545
BstBI TT'CG_AA
Cuts at: 4349 4349
Size: 5545
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5545
BstXI CCAn_nnnn'nTGG
Cuts at: 2317 2317
Size: 5545
BstYI r'GATC_y
Cuts at: 1972 2089 2167 2566 2875 3242 3627 3644 5096
Size: 117 78 399 309 367 385 17 1452
Cuts at: 5096 5182 5194 5540 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 399 385 367 346 309 117
86 78 17 12
Bsu36I CC'TnA_GG
Cuts at: 2676 2676
Size: 5545
BtgI C'CryG_G
Cuts at: 513 1065 2004 2115 2310 2316 2799 513
Size: 552 939 111 195 6 483 3259
Fragments arranged by size:
3259 939 552 483 195 111 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4806
Fragments arranged by size:
4806 683 56
BtsI GCAGTG_nn'
Cuts at: 3061 3855 3875 3061
Size: 794 20 4731
Fragments arranged by size:
4731 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5545
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5510
DraI TTT'AAA
Cuts at: 2849 2849
Size: 5545
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4552 809
Size: 507 3236 1802
Fragments arranged by size:
3236 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 2313 2992
Size: 54 1425 50 275 18 423 60 679
Cuts at: 2992 3881 5510 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 679 423 275 60 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2992 1537
Size: 275 1180 4090
Fragments arranged by size:
4090 1180 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2511 3475 1353
Size: 1158 964 3423
Fragments arranged by size:
3423 1158 964
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4132 4506 4652 387
Size: 1064 138 2543 374 146 1280
Fragments arranged by size:
2543 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2106 2200 2380 2570 2591 2612 3608
Size: 393 474 94 180 190 21 21 996
Cuts at: 3608 4205 4992 5260 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 996 787 597 474 393 268 190
180 94 21 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2819 2819
Size: 5545
EcoO109I rG'GnC_Cy
Cuts at: 2220 2751 2759 2772 2220
Size: 531 8 13 4993
Fragments arranged by size:
4993 531 13 8
EcoRI G'AATT_C
Cuts at: 2854 3232 2854
Size: 378 5167
FspI TGC'GCA
Cuts at: 4051 4051
Size: 5545
HaeII r_GCGC'y
Cuts at: 1678 2499 4692 1678
Size: 821 2193 2531
Fragments arranged by size:
2531 2193 821
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 2204 2236 2795
Size: 32 135 32 509 32 91 32 559
Cuts at: 2795 2827 4186 4218 4260 4292 1373
Size: 32 1359 32 42 32 2626
Fragments arranged by size:
2626 1359 559 509 135 91 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2889 3135 669
Size: 988 281 951 246 3079
Fragments arranged by size:
3079 988 951 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5545
HpaI GTT'AAC
Cuts at: 3135 3135
Size: 5545
KpnI G_GTAC'C
Cuts at: 2870 2870
Size: 5545
MfeI C'AATT_G
Cuts at: 3144 3144
Size: 5545
MluI A'CGCG_T
Cuts at: 5421 5421
Size: 5545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2235 4659 4843 1305
Size: 930 2424 184 2007
Fragments arranged by size:
2424 2007 930 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2255 10
Size: 54 1425 343 423 3300
Fragments arranged by size:
3300 1425 423 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2315 3503 3862 4021 518
Size: 648 1149 1188 359 159 2042
Fragments arranged by size:
2042 1188 1149 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2371 2396 2664 2696 2808 2961 3638 4786
Size: 765 25 268 32 112 153 677 1148
Cuts at: 4786 5031 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 765 677 268 245 153 112
32 25
NcoI C'CATG_G
Cuts at: 513 1065 2004 2316 2799 513
Size: 552 939 312 483 3259
Fragments arranged by size:
3259 939 552 483 312
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5545
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5545
NotI GC'GGCC_GC
Cuts at: 2992 2992
Size: 5545
NruI TCG'CGA
Cuts at: 2023 2023
Size: 5545
NspI r_CATG'y
Cuts at: 1132 1229 2903 4448 1132
Size: 97 1674 1545 2229
Fragments arranged by size:
2229 1674 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2065 2545 1348
Size: 717 480 4348
Fragments arranged by size:
4348 717 480
PciI A'CATG_T
Cuts at: 4444 4444
Size: 5545
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2569 1108
Size: 1461 4084
PfoI T'CCnGG_A
Cuts at: 2421 2421
Size: 5545
PmeI GTTT'AAAC
Cuts at: 2849 2849
Size: 5545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3614 3646 5127 5159 3614
Size: 32 1481 32 4000
Fragments arranged by size:
4000 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2220 2759 2772 2220
Size: 539 13 4993
Fragments arranged by size:
4993 539 13
PshAI GACnn'nnGTC
Cuts at: 1613 2664 1613
Size: 1051 4494
PsiI TTA'TAA
Cuts at: 3115 3115
Size: 5545
PspOMI G'GGCC_C
Cuts at: 2751 2751
Size: 5545
PstI C_TGCA'G
Cuts at: 830 2455 2730 2897 830
Size: 1625 275 167 3478
Fragments arranged by size:
3478 1625 275 167
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5545
PvuII CAG'CTG
Cuts at: 2396 2664 2396
Size: 268 5277
SalI G'TCGA_C
Cuts at: 1936 2887 1936
Size: 951 4594
SanDI GG'GwC_CC
Cuts at: 2772 2772
Size: 5545
SbfI CC_TGCA'GG
Cuts at: 2897 2897
Size: 5545
ScaI AGT'ACT
Cuts at: 3793 5505 3793
Size: 1712 3833
SfcI C'TryA_G
Cuts at: 826 1045 2451 2726 2893 4028 4709 4900 826
Size: 219 1406 275 167 1135 681 191 1471
Fragments arranged by size:
1471 1406 1135 681 275 219 191 167
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5545
SmaI CCC'GGG
Cuts at: 1872 2749 2784 2872 1872
Size: 877 35 88 4545
Fragments arranged by size:
4545 877 88 35
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3649 4550 4812 820
Size: 197 926 1706 901 262 1553
Fragments arranged by size:
1706 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3336 493
Size: 2843 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5545
SphI G_CATG'C
Cuts at: 1132 2903 1132
Size: 1771 3774
SspI AAT'ATT
Cuts at: 5 52 3469 5
Size: 47 3417 2081
Fragments arranged by size:
3417 2081 47
StuI AGG'CCT
Cuts at: 1517 2633 1517
Size: 1116 4429
StyI C'CwwG_G
Cuts at: 513 1065 2004 2171 2316 2755 2799 2984 513
Size: 552 939 167 145 439 44 185 3074
Fragments arranged by size:
3074 939 552 439 185 167 145 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3572 3589 3742 3927 4372 325
Size: 1255 1992 17 153 185 445 1498
Fragments arranged by size:
1992 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3791 5503 96
Size: 274 80 33 51 1443 1814 1712 138
Fragments arranged by size:
1814 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2104 2136 5505 5537 2104
Size: 32 3369 32 2112
Fragments arranged by size:
3369 2112 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2129 1316
Size: 813 4732
XbaI T'CTAG_A
Cuts at: 2881 2881
Size: 5545
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5545
XmaI C'CCGG_G
Cuts at: 1870 2747 2782 2870 1870
Size: 877 35 88 4545
Fragments arranged by size:
4545 877 88 35
XmnI GAAnn'nnTTC
Cuts at: 3674 3674
Size: 5545
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3353 276
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI