Restriction map for
pFN21AB6307
(Circular) (Six-base) MAPSORT of: pFN21AB6307.seq Check: 6172 from: 1 to: 5479
pFN21AB6307 5479 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3289 278
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2822 1937
Size: 885 4594
Acc65I G'GTAC_C
Cuts at: 2800 2800
Size: 5479
AclI AA'CG_TT
Cuts at: 1563 3606 3979 1563
Size: 2043 373 3063
Fragments arranged by size:
3063 2043 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2371 2577 3542 4926 5194 1632
Size: 739 206 965 1384 268 1917
Fragments arranged by size:
1917 1384 965 739 268 206
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5282
AflIII A'CryG_T
Cuts at: 1203 4378 5355 1203
Size: 3175 977 1327
Fragments arranged by size:
3175 1327 977
AgeI A'CCGG_T
Cuts at: 4310 4310
Size: 5479
AhdI GACnn_n'nnGTC
Cuts at: 4208 4208
Size: 5479
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5479
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2015 2047 2015
Size: 32 5447
AlwNI CAG_nnn'CTG
Cuts at: 2555 3222 4794 2555
Size: 667 1572 3240
Fragments arranged by size:
3240 1572 667
ApaLI G'TGCA_C
Cuts at: 3536 4692 3536
Size: 1156 4323
ApoI r'AATT_y
Cuts at: 1493 2330 2788 3014 3166 5275 1493
Size: 837 458 226 152 2109 1697
Fragments arranged by size:
2109 1697 837 458 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4033 160
Size: 872 3001 1606
Fragments arranged by size:
3001 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
AvaI C'yCGr_G
Cuts at: 1870 1943 2804 1870
Size: 73 861 4545
Fragments arranged by size:
4545 861 73
BamHI G'GATC_C
Cuts at: 2809 3176 2809
Size: 367 5112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2050 2059 2135 2434 2800
Size: 325 217 627 263 9 76 299 366
Cuts at: 2800 4255 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 366 325 299 263 217
76 9
BanII G_rGCy'C
Cuts at: 1391 2402 2798 1391
Size: 1011 396 4072
Fragments arranged by size:
4072 1011 396
BbeI G_GCGC'C
Cuts at: 2054 2054
Size: 5479
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4508
BbvCI CC'TCA_GC
Cuts at: 2342 2342
Size: 5479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3670 3704 1903
Size: 34 50 34 1649 34 3678
Fragments arranged by size:
3678 1649 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3371 4581 3371
Size: 1210 4269
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4090 136
Size: 107 122 71 631 605 2418 1525
Fragments arranged by size:
2418 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5474 5474
Size: 5479
BlpI GC'TnA_GC
Cuts at: 2568 2896 2568
Size: 328 5151
Bme1580I G_kGCm'C
Cuts at: 1790 3540 4696 1790
Size: 1750 1156 2573
Fragments arranged by size:
2573 1750 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5479
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3210 4168 442
Size: 416 53 83 399 24 1793 958 1753
Fragments arranged by size:
1793 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2706 4139 1239
Size: 1467 1433 2579
Fragments arranged by size:
2579 1467 1433
Bpu10I CC'TnA_GC
Cuts at: 2342 2342
Size: 5479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3604 4469 4767 3604
Size: 865 298 4316
Fragments arranged by size:
4316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4142 882
Size: 617 2643 2219
Fragments arranged by size:
2643 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3270 493
Size: 711 2066 2702
Fragments arranged by size:
2702 2066 711
BsaBI GATnn'nnATC
Cuts at: 5473 5473
Size: 5479
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2051 2151 3286 3668
Size: 53 83 186 936 518 100 1135 382
Cuts at: 3668 4303 275
Size: 635 1451
Fragments arranged by size:
1451 1135 936 635 518 382 186 100
83 53
BsaWI w'CCGG_w
Cuts at: 2056 3912 4310 4584 4731 5142 2056
Size: 1856 398 274 147 411 2393
Fragments arranged by size:
2393 1856 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2246 2276 1178
Size: 30 165 30 843 30 4381
Fragments arranged by size:
4381 843 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5479
BseYI C'CCAG_C
Cuts at: 4682 5178 4682
Size: 496 4983
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4906
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2041 2929 3690 4718 1540
Size: 275 186 40 888 761 1028 2301
Fragments arranged by size:
2301 1028 888 761 275 186 40
BsiHKAI G_wGCw'C
Cuts at: 3540 3625 4696 3540
Size: 85 1071 4323
Fragments arranged by size:
4323 1071 85
BsmI GAATG_Cn'
Cuts at: 2670 2990 3083 2670
Size: 320 93 5066
Fragments arranged by size:
5066 320 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2402 2798 3540 3625 4696 1391
Size: 399 612 396 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 612 399 396 85
BspEI T'CCGG_A
Cuts at: 5142 5142
Size: 5479
BspHI T'CATG_A
Cuts at: 3368 5098 3368
Size: 1730 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2834 844
Size: 1990 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3366 1394
Size: 429 1543 3507
Fragments arranged by size:
3507 1543 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3974 4148 65
Size: 1183 2726 174 1396
Fragments arranged by size:
2726 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4123 4310 1700
Size: 110 2313 187 2869
Fragments arranged by size:
2869 2313 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5479
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5479
BssSI C'ACGA_G
Cuts at: 3539 4551 3539
Size: 1012 4467
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5479
BstBI TT'CG_AA
Cuts at: 4283 4283
Size: 5479
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5479
BstYI r'GATC_y
Cuts at: 1972 2494 2809 3176 3561 3578 5030 5116 5128
Size: 522 315 367 385 17 1452 86 12
Cuts at: 5128 5474 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 522 385 367 346 315 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2265 513
Size: 552 1200 3727
Fragments arranged by size:
3727 1200 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4740
Fragments arranged by size:
4740 683 56
BtsI GCAGTG_nn'
Cuts at: 2313 2995 3789 3809 2313
Size: 682 794 20 3983
Fragments arranged by size:
3983 794 682 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5444
DraI TTT'AAA
Cuts at: 2783 2783
Size: 5479
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4486 809
Size: 507 3170 1802
Fragments arranged by size:
3170 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2926 3815 5444
Size: 54 1425 50 275 18 1096 889 1629
Cuts at: 5444 8
Size: 43
Fragments arranged by size:
1629 1425 1096 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2926 1537
Size: 275 1114 4090
Fragments arranged by size:
4090 1114 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2553 3409 1353
Size: 1200 856 3423
Fragments arranged by size:
3423 1200 856
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4066 4440 4586 387
Size: 1064 138 2477 374 146 1280
Fragments arranged by size:
2477 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2371 2577 2706 3542 4139 4926 5194
Size: 393 739 206 129 836 597 787 268
Cuts at: 5194 1239
Size: 1524
Fragments arranged by size:
1524 836 787 739 597 393 268 206
129
EcoNI CCTnn'n_nnAGG
Cuts at: 2639 2639
Size: 5479
EcoO109I rG'GnC_Cy
Cuts at: 2023 2023
Size: 5479
EcoRI G'AATT_C
Cuts at: 2788 3166 2788
Size: 378 5101
FspI TGC'GCA
Cuts at: 3985 3985
Size: 5479
HaeII r_GCGC'y
Cuts at: 1678 2054 4626 1678
Size: 376 2572 2531
Fragments arranged by size:
2572 2531 376
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2246 2278 2289 2321 2493
Size: 32 135 32 674 32 11 32 172
Cuts at: 2493 2525 4120 4152 4194 4226 1373
Size: 32 1595 32 42 32 2626
Fragments arranged by size:
2626 1595 674 172 135 42 32 32
32 32 32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2823 3069 669
Size: 988 281 885 246 3079
Fragments arranged by size:
3079 988 885 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5479
HpaI GTT'AAC
Cuts at: 3069 3069
Size: 5479
KasI G'GCGC_C
Cuts at: 2050 2050
Size: 5479
KpnI G_GTAC'C
Cuts at: 2804 2804
Size: 5479
MfeI C'AATT_G
Cuts at: 3078 3078
Size: 5479
MluI A'CGCG_T
Cuts at: 5355 5355
Size: 5479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2326 2461 2472 2508 4593 4777 1305
Size: 1021 135 11 36 2085 184 2007
Fragments arranged by size:
2085 2007 1021 184 135 36 11
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3657
Fragments arranged by size:
3657 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3437 3796 3955 518
Size: 648 2271 359 159 2042
Fragments arranged by size:
2271 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2043 2267 2895 3572 4720 4965 1606
Size: 437 224 628 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 628 437 245 224
NarI GG'CG_CC
Cuts at: 2051 2051
Size: 5479
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4927
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5479
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5479
NotI GC'GGCC_GC
Cuts at: 2926 2926
Size: 5479
NspI r_CATG'y
Cuts at: 1132 1229 2837 4382 1132
Size: 97 1608 1545 2229
Fragments arranged by size:
2229 1608 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5479
PciI A'CATG_T
Cuts at: 4378 4378
Size: 5479
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2751 1108
Size: 1643 3836
PmeI GTTT'AAAC
Cuts at: 2783 2783
Size: 5479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 5061 5093 3548
Size: 32 1481 32 3934
Fragments arranged by size:
3934 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2023 2023
Size: 5479
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5479
PsiI TTA'TAA
Cuts at: 3049 3049
Size: 5479
PstI C_TGCA'G
Cuts at: 830 2831 830
Size: 2001 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5479
SacII CC_GC'GG
Cuts at: 2268 2268
Size: 5479
SalI G'TCGA_C
Cuts at: 1936 2821 1936
Size: 885 4594
SanDI GG'GwC_CC
Cuts at: 2023 2023
Size: 5479
SbfI CC_TGCA'GG
Cuts at: 2831 2831
Size: 5479
ScaI AGT'ACT
Cuts at: 2765 3727 5439 2765
Size: 962 1712 2805
Fragments arranged by size:
2805 1712 962
SfcI C'TryA_G
Cuts at: 826 1045 2827 3962 4643 4834 826
Size: 219 1782 1135 681 191 1471
Fragments arranged by size:
1782 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2052 2052
Size: 5479
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5479
SmaI CCC'GGG
Cuts at: 1872 2806 1872
Size: 934 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3583 4484 4746 820
Size: 197 926 1640 901 262 1553
Fragments arranged by size:
1640 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3270 493
Size: 2777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5479
SphI G_CATG'C
Cuts at: 1132 2837 1132
Size: 1705 3774
SspI AAT'ATT
Cuts at: 5 52 3403 5
Size: 47 3351 2081
Fragments arranged by size:
3351 2081 47
StuI AGG'CCT
Cuts at: 1517 2347 1517
Size: 830 4649
StyI C'CwwG_G
Cuts at: 513 1065 2918 513
Size: 552 1853 3074
Fragments arranged by size:
3074 1853 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2422 3506 3523 3676 3861 4306 325
Size: 1255 842 1084 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1084 842 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 2763 3725
Size: 274 80 33 51 1443 258 528 962
Cuts at: 3725 5437 96
Size: 1712 138
Fragments arranged by size:
1712 1443 962 528 274 258 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5439 5471 5439
Size: 32 5447
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5479
XbaI T'CTAG_A
Cuts at: 2815 2815
Size: 5479
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5479
XmaI C'CCGG_G
Cuts at: 1870 2804 1870
Size: 934 4545
XmnI GAAnn'nnTTC
Cuts at: 3608 3608
Size: 5479
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3287 276
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SapI SexAI SfiI SrfI SwaI XcmI