Restriction map for
pFN21AB6306
(Circular) (Six-base) MAPSORT of: pFN21AB6306.seq Check: 9256 from: 1 to: 5545
pFN21AB6306 5545 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2255 2255
Size: 5545
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3355 278
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2888 1937
Size: 951 4594
Acc65I G'GTAC_C
Cuts at: 2866 2866
Size: 5545
AclI AA'CG_TT
Cuts at: 1563 3672 4045 1563
Size: 2109 373 3063
Fragments arranged by size:
3063 2109 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3608 4992 5260 1632
Size: 1976 1384 268 1917
Fragments arranged by size:
1976 1917 1384 268
AfeI AGC'GCT
Cuts at: 2222 2222
Size: 5545
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5348
AflIII A'CryG_T
Cuts at: 1203 4444 5421 1203
Size: 3241 977 1327
Fragments arranged by size:
3241 1327 977
AgeI A'CCGG_T
Cuts at: 4376 4376
Size: 5545
AhdI GACnn_n'nnGTC
Cuts at: 4274 4274
Size: 5545
AleI CACnn'nnGTG
Cuts at: 1166 2662 1166
Size: 1496 4049
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2680 1184
Size: 1496 4049
AlwNI CAG_nnn'CTG
Cuts at: 2397 2410 2673 3288 4860 2397
Size: 13 263 615 1572 3082
Fragments arranged by size:
3082 1572 615 263 13
ApaI G_GGCC'C
Cuts at: 2844 2844
Size: 5545
ApaLI G'TGCA_C
Cuts at: 3602 4758 3602
Size: 1156 4389
ApoI r'AATT_y
Cuts at: 1493 2854 3080 3232 5341 1493
Size: 1361 226 152 2109 1697
Fragments arranged by size:
2109 1697 1361 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4099 160
Size: 872 3067 1606
Fragments arranged by size:
3067 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
AvaI C'yCGr_G
Cuts at: 1870 1943 2155 2610 2870 1870
Size: 73 212 455 260 4545
Fragments arranged by size:
4545 455 260 212 73
BamHI G'GATC_C
Cuts at: 2875 3242 2875
Size: 367 5178
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2736 2866 4321 618
Size: 325 217 627 949 130 1455 1842
Fragments arranged by size:
1842 1455 949 627 325 217 130
BanII G_rGCy'C
Cuts at: 1391 2417 2844 2864 1391
Size: 1026 427 20 4072
Fragments arranged by size:
4072 1026 427 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2181 2730 928
Size: 971 282 549 3743
Fragments arranged by size:
3743 971 549 282
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2423 2457 3736 3770 1903
Size: 34 486 34 1279 34 3678
Fragments arranged by size:
3678 1279 486 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3437 4647 3437
Size: 1210 4335
BclI T'GATC_A
Cuts at: 1548 2536 1548
Size: 988 4557
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4156 136
Size: 107 122 71 631 605 2484 1525
Fragments arranged by size:
2484 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5540 5540
Size: 5545
BlpI GC'TnA_GC
Cuts at: 2962 2962
Size: 5545
Bme1580I G_kGCm'C
Cuts at: 1790 2094 2433 2460 2844 3606 4762 1790
Size: 304 339 27 384 762 1156 2573
Fragments arranged by size:
2573 1156 762 384 339 304 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5545
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2420 3276 4234
Size: 416 53 83 399 24 1003 856 958
Cuts at: 4234 442
Size: 1753
Fragments arranged by size:
1753 1003 958 856 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4205 1239
Size: 2966 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3670 4535 4833 3670
Size: 865 298 4382
Fragments arranged by size:
4382 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2799 4208 882
Size: 617 1300 1409 2219
Fragments arranged by size:
2219 1409 1300 617
BsaAI yAC'GTr
Cuts at: 493 1204 3336 493
Size: 711 2132 2702
Fragments arranged by size:
2702 2132 711
BsaBI GATnn'nnATC
Cuts at: 2619 5539 2619
Size: 2920 2625
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2582 3352 3734 4369
Size: 53 83 186 936 1049 770 382 635
Cuts at: 4369 275
Size: 1451
Fragments arranged by size:
1451 1049 936 770 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2570 2797 3978 4376 4650 4797 5208 2570
Size: 227 1181 398 274 147 411 2907
Fragments arranged by size:
2907 1181 411 398 274 227 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2735 2765 2796 2826 1178
Size: 30 165 30 1332 30 31 30 3897
Fragments arranged by size:
3897 1332 165 31 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2472 1186
Size: 1286 4259
BseYI C'CCAG_C
Cuts at: 4748 5244 4748
Size: 496 5049
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2410 2686 1163
Size: 573 674 276 4022
Fragments arranged by size:
4022 674 573 276
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2995 3756 4784 1540
Size: 275 186 994 761 1028 2301
Fragments arranged by size:
2301 1028 994 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2309 2379 2446 2817 3606 3691 4762 2309
Size: 70 67 371 789 85 1071 3092
Fragments arranged by size:
3092 1071 789 371 85 70 67
BsmI GAATG_Cn'
Cuts at: 3056 3149 3056
Size: 93 5452
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2309 2379 2417 2433 2446 2460
Size: 399 304 215 70 38 16 13 14
Cuts at: 2460 2817 2844 2864 3606 3691 4762 1391
Size: 357 27 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 357 304 215 85
70 38 27 20 16 14 13
BspEI T'CCGG_A
Cuts at: 5208 5208
Size: 5545
BspHI T'CATG_A
Cuts at: 3434 5164 3434
Size: 1730 3815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2255 2900 844
Size: 1411 645 3489
Fragments arranged by size:
3489 1411 645
BsrBI CCG'CTC
Cuts at: 1394 1823 3432 1394
Size: 429 1609 3507
Fragments arranged by size:
3507 1609 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2278 4040 4214 65
Size: 1183 1030 1762 174 1396
Fragments arranged by size:
1762 1396 1183 1030 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4189 4376 1700
Size: 110 2379 187 2869
Fragments arranged by size:
2869 2379 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5545
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5545
BssSI C'ACGA_G
Cuts at: 2189 3605 4617 2189
Size: 1416 1012 3117
Fragments arranged by size:
3117 1416 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2277 2673 794
Size: 1483 396 3666
Fragments arranged by size:
3666 1483 396
BstBI TT'CG_AA
Cuts at: 4349 4349
Size: 5545
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5545
BstYI r'GATC_y
Cuts at: 1972 2620 2875 3242 3627 3644 5096 5182 5194
Size: 648 255 367 385 17 1452 86 12
Cuts at: 5194 5540 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 648 385 367 346 255 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2543 513
Size: 552 1478 3515
Fragments arranged by size:
3515 1478 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4806
Fragments arranged by size:
4806 683 56
BtsI GCAGTG_nn'
Cuts at: 2293 2309 2377 2398 3061 3855 3875 2293
Size: 16 68 21 663 794 20 3963
Fragments arranged by size:
3963 794 663 68 21 20 16
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5545
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5510
DraI TTT'AAA
Cuts at: 2849 2849
Size: 5545
DraIII CAC_nnn'GTG
Cuts at: 2519 2519
Size: 5545
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4552 809
Size: 507 3236 1802
Fragments arranged by size:
3236 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2992 3881 5510
Size: 54 1425 50 275 18 1162 889 1629
Cuts at: 5510 8
Size: 43
Fragments arranged by size:
1629 1425 1162 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2992 1537
Size: 275 1180 4090
Fragments arranged by size:
4090 1180 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3475 1353
Size: 2122 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4132 4506 4652 387
Size: 1064 138 2543 374 146 1280
Fragments arranged by size:
2543 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3608 4205 4992 5260 1239
Size: 393 1976 597 787 268 1524
Fragments arranged by size:
1976 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2337 2840 2337
Size: 503 5042
EcoRI G'AATT_C
Cuts at: 2854 3232 2854
Size: 378 5167
FspI TGC'GCA
Cuts at: 4051 4051
Size: 5545
HaeII r_GCGC'y
Cuts at: 1678 2224 2683 4692 1678
Size: 546 459 2009 2531
Fragments arranged by size:
2531 2009 546 459
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4186 4218 4260 4292 1373
Size: 32 135 32 2614 32 42 32 2626
Fragments arranged by size:
2626 2614 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2889 3135 669
Size: 988 281 951 246 3079
Fragments arranged by size:
3079 988 951 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5545
HpaI GTT'AAC
Cuts at: 3135 3135
Size: 5545
KpnI G_GTAC'C
Cuts at: 2870 2870
Size: 5545
MfeI C'AATT_G
Cuts at: 3144 3144
Size: 5545
MluI A'CGCG_T
Cuts at: 5421 5421
Size: 5545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4659 4843 1305
Size: 3354 184 2007
Fragments arranged by size:
3354 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3723
Fragments arranged by size:
3723 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2662 3503 3862 4021 518
Size: 648 1496 841 359 159 2042
Fragments arranged by size:
2042 1496 841 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2076 2205 2214 2496 2673 2763 2961 3638
Size: 470 129 9 282 177 90 198 677
Cuts at: 3638 4786 5031 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 470 282 245 198 177
129 90 9
NcoI C'CATG_G
Cuts at: 513 1065 2543 513
Size: 552 1478 3515
Fragments arranged by size:
3515 1478 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5545
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5545
NotI GC'GGCC_GC
Cuts at: 2992 2992
Size: 5545
NspI r_CATG'y
Cuts at: 1132 1229 2903 4448 1132
Size: 97 1674 1545 2229
Fragments arranged by size:
2229 1674 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5545
PciI A'CATG_T
Cuts at: 4444 4444
Size: 5545
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5545
PfoI T'CCnGG_A
Cuts at: 2339 2339
Size: 5545
PmeI GTTT'AAAC
Cuts at: 2849 2849
Size: 5545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3614 3646 5127 5159 3614
Size: 32 1481 32 4000
Fragments arranged by size:
4000 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2337 2337
Size: 5545
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5545
PsiI TTA'TAA
Cuts at: 3115 3115
Size: 5545
PspOMI G'GGCC_C
Cuts at: 2840 2840
Size: 5545
PspXI vC'TCGA_Gb
Cuts at: 2155 2155
Size: 5545
PstI C_TGCA'G
Cuts at: 830 2288 2501 2897 830
Size: 1458 213 396 3478
Fragments arranged by size:
3478 1458 396 213
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5545
PvuII CAG'CTG
Cuts at: 2673 2673
Size: 5545
SalI G'TCGA_C
Cuts at: 1936 2887 1936
Size: 951 4594
SanDI GG'GwC_CC
Cuts at: 2337 2337
Size: 5545
SbfI CC_TGCA'GG
Cuts at: 2897 2897
Size: 5545
ScaI AGT'ACT
Cuts at: 3793 5505 3793
Size: 1712 3833
SfcI C'TryA_G
Cuts at: 826 1045 2284 2497 2827 2893 4028 4709 4900
Size: 219 1239 213 330 66 1135 681 191
Cuts at: 4900 826
Size: 1471
Fragments arranged by size:
1471 1239 1135 681 330 219 213 191
66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5545
SmaI CCC'GGG
Cuts at: 1872 2612 2872 1872
Size: 740 260 4545
Fragments arranged by size:
4545 740 260
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2155 3649 4550 4812 820
Size: 197 926 212 1494 901 262 1553
Fragments arranged by size:
1553 1494 926 901 262 212 197
SnaBI TAC'GTA
Cuts at: 493 3336 493
Size: 2843 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5545
SphI G_CATG'C
Cuts at: 1132 2903 1132
Size: 1771 3774
SspI AAT'ATT
Cuts at: 5 52 3469 5
Size: 47 3417 2081
Fragments arranged by size:
3417 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5545
StyI C'CwwG_G
Cuts at: 513 1065 2543 2984 513
Size: 552 1478 441 3074
Fragments arranged by size:
3074 1478 552 441
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2742 3572 3589 3742 3927 4372 325
Size: 1255 1162 830 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1162 830 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2058 3791 5503
Size: 274 80 33 51 1443 81 1733 1712
Cuts at: 5503 96
Size: 138
Fragments arranged by size:
1733 1712 1443 274 138 81 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2796 2828 5505 5537 2796
Size: 32 2677 32 2804
Fragments arranged by size:
2804 2677 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5545
XbaI T'CTAG_A
Cuts at: 2881 2881
Size: 5545
XhoI C'TCGA_G
Cuts at: 1943 2155 1943
Size: 212 5333
XmaI C'CCGG_G
Cuts at: 1870 2610 2870 1870
Size: 740 260 4545
Fragments arranged by size:
4545 740 260
XmnI GAAnn'nnTTC
Cuts at: 3674 3674
Size: 5545
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3353 276
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PsrI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI XcmI