Restriction map for
pFN21AB6304
(Circular) (Six-base) MAPSORT of: pFN21AB6304.seq Check: 563 from: 1 to: 5539
pFN21AB6304 5539 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3349 278
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2157 2473 2510 2823 2882 1937
Size: 220 316 37 313 59 4594
Fragments arranged by size:
4594 316 313 220 59 37
Acc65I G'GTAC_C
Cuts at: 2860 2860
Size: 5539
AclI AA'CG_TT
Cuts at: 1563 3666 4039 1563
Size: 2103 373 3063
Fragments arranged by size:
3063 2103 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3602 4986 5254 1632
Size: 1970 1384 268 1917
Fragments arranged by size:
1970 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5342
AflIII A'CryG_T
Cuts at: 1203 4438 5415 1203
Size: 3235 977 1327
Fragments arranged by size:
3235 1327 977
AgeI A'CCGG_T
Cuts at: 4370 4370
Size: 5539
AhdI GACnn_n'nnGTC
Cuts at: 4268 4268
Size: 5539
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5539
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2055 1184
Size: 871 4668
AlwNI CAG_nnn'CTG
Cuts at: 3282 4854 3282
Size: 1572 3967
ApaLI G'TGCA_C
Cuts at: 3596 4752 3596
Size: 1156 4383
ApoI r'AATT_y
Cuts at: 1493 2245 2440 2576 2848 3074 3226 5335 1493
Size: 752 195 136 272 226 152 2109 1697
Fragments arranged by size:
2109 1697 752 272 226 195 152 136
AseI AT'TA_AT
Cuts at: 160 1032 4093 160
Size: 872 3061 1606
Fragments arranged by size:
3061 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
AvaI C'yCGr_G
Cuts at: 1870 1943 2864 1870
Size: 73 921 4545
Fragments arranged by size:
4545 921 73
BamHI G'GATC_C
Cuts at: 2869 3236 2869
Size: 367 5172
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2860 4315 618
Size: 325 217 627 1073 1455 1842
Fragments arranged by size:
1842 1455 1073 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2858 1391
Size: 1467 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4568
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3730 3764 1903
Size: 34 1793 34 3678
Fragments arranged by size:
3678 1793 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2323 3431 4641 2323
Size: 1108 1210 3221
Fragments arranged by size:
3221 1210 1108
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5539
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4150 136
Size: 107 122 71 631 605 2478 1525
Fragments arranged by size:
2478 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5534 5534
Size: 5539
BlpI GC'TnA_GC
Cuts at: 2956 2956
Size: 5539
Bme1580I G_kGCm'C
Cuts at: 1790 3600 4756 1790
Size: 1810 1156 2573
Fragments arranged by size:
2573 1810 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5539
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3270 4228 442
Size: 416 53 83 399 24 1853 958 1753
Fragments arranged by size:
1853 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2342 1056
Size: 1286 4253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4199 1239
Size: 2960 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2742 3664 4529 4827 2742
Size: 922 865 298 3454
Fragments arranged by size:
3454 922 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4202 882
Size: 617 2703 2219
Fragments arranged by size:
2703 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3330 493
Size: 711 2126 2702
Fragments arranged by size:
2702 2126 711
BsaBI GATnn'nnATC
Cuts at: 5533 5533
Size: 5539
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3346 3728 4363 275
Size: 53 83 186 936 1813 382 635 1451
Fragments arranged by size:
1813 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3972 4370 4644 4791 5202 3972
Size: 398 274 147 411 4309
Fragments arranged by size:
4309 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5314
Fragments arranged by size:
5314 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2618 1186
Size: 847 585 4107
Fragments arranged by size:
4107 847 585
BseYI C'CCAG_C
Cuts at: 4742 5238 4742
Size: 496 5043
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4966
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2989 3750 4778 1540
Size: 275 186 988 761 1028 2301
Fragments arranged by size:
2301 1028 988 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3600 3685 4756 3600
Size: 85 1071 4383
Fragments arranged by size:
4383 1071 85
BsmI GAATG_Cn'
Cuts at: 2296 3050 3143 2296
Size: 754 93 4692
Fragments arranged by size:
4692 754 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2754 2754
Size: 5539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2858 3600 3685 4756 1391
Size: 399 1068 742 85 1071 2174
Fragments arranged by size:
2174 1071 1068 742 399 85
BspEI T'CCGG_A
Cuts at: 5202 5202
Size: 5539
BspHI T'CATG_A
Cuts at: 3428 5158 3428
Size: 1730 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2023 2894 844
Size: 1179 871 3489
Fragments arranged by size:
3489 1179 871
BsrBI CCG'CTC
Cuts at: 1394 1823 3426 1394
Size: 429 1603 3507
Fragments arranged by size:
3507 1603 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4034 4208 65
Size: 1183 2786 174 1396
Fragments arranged by size:
2786 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4183 4370 1700
Size: 110 2373 187 2869
Fragments arranged by size:
2869 2373 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5539
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5539
BssSI C'ACGA_G
Cuts at: 3599 4611 3599
Size: 1012 4527
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5539
BstBI TT'CG_AA
Cuts at: 4343 4343
Size: 5539
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5539
BstXI CCAn_nnnn'nTGG
Cuts at: 2626 2626
Size: 5539
BstYI r'GATC_y
Cuts at: 1972 2869 3236 3621 3638 5090 5176 5188 5534
Size: 897 367 385 17 1452 86 12 346
Cuts at: 5534 1972
Size: 1977
Fragments arranged by size:
1977 1452 897 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2096 513
Size: 552 939 92 3956
Fragments arranged by size:
3956 939 552 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4800
Fragments arranged by size:
4800 683 56
BtsI GCAGTG_nn'
Cuts at: 2364 3055 3849 3869 2364
Size: 691 794 20 4034
Fragments arranged by size:
4034 794 691 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5539
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5504
DraI TTT'AAA
Cuts at: 2843 2843
Size: 5539
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2153 4546 809
Size: 507 837 2393 1802
Fragments arranged by size:
2393 1802 837 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2986 3875 5504
Size: 54 1425 50 275 18 1156 889 1629
Cuts at: 5504 8
Size: 43
Fragments arranged by size:
1629 1425 1156 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2986 1537
Size: 275 1174 4090
Fragments arranged by size:
4090 1174 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2812 3469 1353
Size: 1459 657 3423
Fragments arranged by size:
3423 1459 657
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4126 4500 4646 387
Size: 1064 138 2537 374 146 1280
Fragments arranged by size:
2537 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3602 4199 4986 5254 1239
Size: 393 1970 597 787 268 1524
Fragments arranged by size:
1970 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2576 2848 3226 2576
Size: 272 378 4889
Fragments arranged by size:
4889 378 272
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2825 2825
Size: 5539
FspI TGC'GCA
Cuts at: 4045 4045
Size: 5539
HaeII r_GCGC'y
Cuts at: 1678 4686 1678
Size: 3008 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2101 2133 2357 2362 2389
Size: 32 135 32 529 32 224 5 27
Cuts at: 2389 2394 2574 2606 4180 4212 4254 4286 1373
Size: 5 180 32 1574 32 42 32 2626
Fragments arranged by size:
2626 1574 529 224 180 135 42 32
32 32 32 32 32 27 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2302 2883 3129 669
Size: 988 281 364 581 246 3079
Fragments arranged by size:
3079 988 581 364 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5539
HpaI GTT'AAC
Cuts at: 3129 3129
Size: 5539
KpnI G_GTAC'C
Cuts at: 2864 2864
Size: 5539
MfeI C'AATT_G
Cuts at: 3138 3138
Size: 5539
MluI A'CGCG_T
Cuts at: 5415 5415
Size: 5539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4653 4837 1305
Size: 3348 184 2007
Fragments arranged by size:
3348 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3717
Fragments arranged by size:
3717 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3497 3856 4015 518
Size: 648 2331 359 159 2042
Fragments arranged by size:
2331 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2955 3632 4780 5025 1606
Size: 1349 677 1148 245 2120
Fragments arranged by size:
2120 1349 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4048
Fragments arranged by size:
4048 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5539
NheI G'CTAG_C
Cuts at: 1052 2338 1052
Size: 1286 4253
NotI GC'GGCC_GC
Cuts at: 2986 2986
Size: 5539
NspI r_CATG'y
Cuts at: 1132 1229 2897 4442 1132
Size: 97 1668 1545 2229
Fragments arranged by size:
2229 1668 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5539
PciI A'CATG_T
Cuts at: 4438 4438
Size: 5539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5539
PfoI T'CCnGG_A
Cuts at: 2142 2142
Size: 5539
PmeI GTTT'AAAC
Cuts at: 2843 2843
Size: 5539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 5121 5153 3608
Size: 32 1481 32 3994
Fragments arranged by size:
3994 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5539
PsiI TTA'TAA
Cuts at: 3109 3109
Size: 5539
PstI C_TGCA'G
Cuts at: 830 2359 2602 2891 830
Size: 1529 243 289 3478
Fragments arranged by size:
3478 1529 289 243
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5539
SalI G'TCGA_C
Cuts at: 1936 2881 1936
Size: 945 4594
SbfI CC_TGCA'GG
Cuts at: 2891 2891
Size: 5539
ScaI AGT'ACT
Cuts at: 3787 5499 3787
Size: 1712 3827
SexAI A'CCwGG_T
Cuts at: 2160 2160
Size: 5539
SfcI C'TryA_G
Cuts at: 826 1045 2355 2511 2598 2887 4022 4703 4894
Size: 219 1310 156 87 289 1135 681 191
Cuts at: 4894 826
Size: 1471
Fragments arranged by size:
1471 1310 1135 681 289 219 191 156
87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5539
SmaI CCC'GGG
Cuts at: 1872 2866 1872
Size: 994 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2757 3643 4544 4806 820
Size: 197 926 814 886 901 262 1553
Fragments arranged by size:
1553 926 901 886 814 262 197
SnaBI TAC'GTA
Cuts at: 493 3330 493
Size: 2837 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5539
SphI G_CATG'C
Cuts at: 1132 2897 1132
Size: 1765 3774
SspI AAT'ATT
Cuts at: 5 52 2058 3463 5
Size: 47 2006 1405 2081
Fragments arranged by size:
2081 2006 1405 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5539
StyI C'CwwG_G
Cuts at: 513 1065 2004 2978 513
Size: 552 939 974 3074
Fragments arranged by size:
3074 974 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3566 3583 3736 3921 4366 325
Size: 1255 1986 17 153 185 445 1498
Fragments arranged by size:
1986 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3785 5497 96
Size: 274 80 33 51 1443 1808 1712 138
Fragments arranged by size:
1808 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5499 5531 5499
Size: 32 5507
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5539
XbaI T'CTAG_A
Cuts at: 2875 2875
Size: 5539
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5539
XmaI C'CCGG_G
Cuts at: 1870 2864 1870
Size: 994 4545
XmnI GAAnn'nnTTC
Cuts at: 3668 3668
Size: 5539
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3347 276
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI