Restriction map for
pFN21AB6295
(Circular) (Six-base) MAPSORT of: pFN21AB6295.seq Check: 6047 from: 1 to: 7129
pFN21AB6295 7129 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3083 3083
Size: 7129
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4939 278
Size: 53 83 186 936 3403 2468
Fragments arranged by size:
3403 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2689 2873 3618 3842 4472 1937
Size: 752 184 745 224 630 4594
Fragments arranged by size:
4594 752 745 630 224 184
Acc65I G'GTAC_C
Cuts at: 4450 4450
Size: 7129
AclI AA'CG_TT
Cuts at: 1563 5256 5629 1563
Size: 3693 373 3063
Fragments arranged by size:
3693 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2730 2747 3081 5192 6576 6844 1632
Size: 1098 17 334 2111 1384 268 1917
Fragments arranged by size:
2111 1917 1384 1098 334 268 17
AfeI AGC'GCT
Cuts at: 2171 2171
Size: 7129
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6932
AflIII A'CryG_T
Cuts at: 1203 6028 7005 1203
Size: 4825 977 1327
Fragments arranged by size:
4825 1327 977
AgeI A'CCGG_T
Cuts at: 5960 5960
Size: 7129
AhdI GACnn_n'nnGTC
Cuts at: 3853 5858 3853
Size: 2005 5124
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2480 4195 1184
Size: 1296 1715 4118
Fragments arranged by size:
4118 1715 1296
AlwNI CAG_nnn'CTG
Cuts at: 2068 3578 4004 4872 6444 2068
Size: 1510 426 868 1572 2753
Fragments arranged by size:
2753 1572 1510 868 426
ApaLI G'TGCA_C
Cuts at: 3189 5186 6342 3189
Size: 1997 1156 3976
Fragments arranged by size:
3976 1997 1156
ApoI r'AATT_y
Cuts at: 1493 2660 2770 3126 3428 4438 4664 4816 6925
Size: 1167 110 356 302 1010 226 152 2109
Cuts at: 6925 1493
Size: 1697
Fragments arranged by size:
2109 1697 1167 1010 356 302 226 152
110
AseI AT'TA_AT
Cuts at: 160 1032 5683 160
Size: 872 4651 1606
Fragments arranged by size:
4651 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
AvaI C'yCGr_G
Cuts at: 1870 1943 2747 4069 4454 1870
Size: 73 804 1322 385 4545
Fragments arranged by size:
4545 1322 804 385 73
AvrII C'CTAG_G
Cuts at: 2890 3079 2890
Size: 189 6940
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2815 2848 2815
Size: 33 7096
BamHI G'GATC_C
Cuts at: 4459 4826 4459
Size: 367 6762
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4130 4330 4450 5905 618
Size: 325 217 627 2343 200 120 1455 1842
Fragments arranged by size:
2343 1842 1455 627 325 217 200 120
BanII G_rGCy'C
Cuts at: 1391 2054 3456 3654 4448 1391
Size: 663 1402 198 794 4072
Fragments arranged by size:
4072 1402 794 663 198
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2166 2220 3069 3713 4173 4419 928
Size: 971 267 54 849 644 460 246 3638
Fragments arranged by size:
3638 971 849 644 460 267 246 54
BbvCI CC'TCA_GC
Cuts at: 4005 4005
Size: 7129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5320 5354 1903
Size: 34 3383 34 3678
Fragments arranged by size:
3678 3383 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2816 5021 6231 2816
Size: 2205 1210 3714
Fragments arranged by size:
3714 2205 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7129
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2327 5740 136
Size: 107 122 71 631 605 655 3413 1525
Fragments arranged by size:
3413 1525 655 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7124 7124
Size: 7129
BlpI GC'TnA_GC
Cuts at: 2541 3450 4546 2541
Size: 909 1096 5124
Fragments arranged by size:
5124 1096 909
Bme1580I G_kGCm'C
Cuts at: 1790 3193 4353 5190 6346 1790
Size: 1403 1160 837 1156 2573
Fragments arranged by size:
2573 1403 1160 1156 837
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7129
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3743 4860 5818
Size: 416 53 83 399 24 2326 1117 958
Cuts at: 5818 442
Size: 1753
Fragments arranged by size:
2326 1753 1117 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2191 3159 2191
Size: 968 6161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2159 2624 3552 5789 1239
Size: 920 465 928 2237 2579
Fragments arranged by size:
2579 2237 928 920 465
Bpu10I CC'TnA_GC
Cuts at: 3088 4005 3088
Size: 917 6212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2852 3152 3239 5254 6119 6417 2852
Size: 300 87 2015 865 298 3564
Fragments arranged by size:
3564 2015 865 300 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2257 2298 5792 882
Size: 617 758 41 3494 2219
Fragments arranged by size:
3494 2219 758 617 41
BsaAI yAC'GTr
Cuts at: 493 1204 4920 493
Size: 711 3716 2702
Fragments arranged by size:
3716 2702 711
BsaBI GATnn'nnATC
Cuts at: 7123 7123
Size: 7129
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4936 5318 5953 275
Size: 53 83 186 936 3403 382 635 1451
Fragments arranged by size:
3403 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3937 5562 5960 6234 6381 6792 3937
Size: 1625 398 274 147 411 4274
Fragments arranged by size:
4274 1625 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3196 3226 1178
Size: 30 165 30 1793 30 5081
Fragments arranged by size:
5081 1793 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4022 4398 1186
Size: 2836 376 3917
Fragments arranged by size:
3917 2836 376
BseYI C'CCAG_C
Cuts at: 6332 6828 6332
Size: 496 6633
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4165 1163
Size: 573 2429 4127
Fragments arranged by size:
4127 2429 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2022 4579 5340 6368 1540
Size: 275 186 21 2557 761 1028 2301
Fragments arranged by size:
2557 2301 1028 761 275 186 21
BsiHKAI G_wGCw'C
Cuts at: 3193 5190 5275 6346 3193
Size: 1997 85 1071 3976
Fragments arranged by size:
3976 1997 1071 85
BsmI GAATG_Cn'
Cuts at: 4640 4733 4640
Size: 93 7036
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 3193 3456 3654 4353 4448 5190
Size: 399 264 1139 263 198 699 95 742
Cuts at: 5190 5275 6346 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1139 1071 742 699 399 264 263
198 95 85
BspEI T'CCGG_A
Cuts at: 3937 6792 3937
Size: 2855 4274
BspHI T'CATG_A
Cuts at: 3358 5018 6748 3358
Size: 1660 1730 3739
Fragments arranged by size:
3739 1730 1660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3083 3823 4484 844
Size: 2239 740 661 3489
Fragments arranged by size:
3489 2239 740 661
BsrBI CCG'CTC
Cuts at: 1394 1823 2024 2143 5016 1394
Size: 429 201 119 2873 3507
Fragments arranged by size:
3507 2873 429 201 119
BsrDI GCAATG_nn'
Cuts at: 65 1248 5624 5798 65
Size: 1183 4376 174 1396
Fragments arranged by size:
4376 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5773 5960 1700
Size: 110 3963 187 2869
Fragments arranged by size:
3963 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3991 96
Size: 3895 3234
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7129
BssSI C'ACGA_G
Cuts at: 3322 5189 6201 3322
Size: 1867 1012 4250
Fragments arranged by size:
4250 1867 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7129
BstBI TT'CG_AA
Cuts at: 5933 5933
Size: 7129
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7129
BstXI CCAn_nnnn'nTGG
Cuts at: 2908 2908
Size: 7129
BstYI r'GATC_y
Cuts at: 1972 2121 2183 4459 4826 5211 5228 6680 6766
Size: 149 62 2276 367 385 17 1452 86
Cuts at: 6766 6778 7124 1972
Size: 12 346 1977
Fragments arranged by size:
2276 1977 1452 385 367 346 149 86
62 17 12
BstZ17I GTA'TAC
Cuts at: 2690 2690
Size: 7129
Bsu36I CC'TnA_GG
Cuts at: 2249 2249
Size: 7129
BtgI C'CryG_G
Cuts at: 513 1065 2016 513
Size: 552 951 5626
Fragments arranged by size:
5626 951 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3681 487
Size: 683 56 2455 3935
Fragments arranged by size:
3935 2455 683 56
BtsI GCAGTG_nn'
Cuts at: 3066 3291 3867 3925 4645 5439 5459 3066
Size: 225 576 58 720 794 20 4736
Fragments arranged by size:
4736 794 720 576 225 58 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7129
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7094
DraI TTT'AAA
Cuts at: 4433 4433
Size: 7129
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6136 809
Size: 507 4820 1802
Fragments arranged by size:
4820 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 4576 5465
Size: 54 1425 50 275 18 189 2557 889
Cuts at: 5465 7094 8
Size: 1629 43
Fragments arranged by size:
2557 1629 1425 889 275 189 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2019 4576 1537
Size: 275 207 2557 4090
Fragments arranged by size:
4090 2557 275 207
EarI CTCTTCn'nnn_
Cuts at: 1353 2253 2502 2640 2847 2890 3294 3501 3822
Size: 900 249 138 207 43 404 207 321
Cuts at: 3822 4333 5059 1353
Size: 511 726 3423
Fragments arranged by size:
3423 900 726 511 404 321 249 207
207 138 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2087 5716 6090 6236 387
Size: 1064 138 498 3629 374 146 1280
Fragments arranged by size:
3629 1280 1064 498 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2624 2730 2747 3081 3552 5192
Size: 393 527 465 106 17 334 471 1640
Cuts at: 5192 5789 6576 6844 1239
Size: 597 787 268 1524
Fragments arranged by size:
1640 1524 787 597 527 471 465 393
334 268 106 17
EcoO109I rG'GnC_Cy
Cuts at: 2722 2831 2886 3382 2722
Size: 109 55 496 6469
Fragments arranged by size:
6469 496 109 55
EcoRI G'AATT_C
Cuts at: 3428 4438 4816 3428
Size: 1010 378 5741
Fragments arranged by size:
5741 1010 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 7129
FspI TGC'GCA
Cuts at: 5635 5635
Size: 7129
HaeII r_GCGC'y
Cuts at: 1678 2173 6276 1678
Size: 495 4103 2531
Fragments arranged by size:
4103 2531 495
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2210 2242 2276 2308 2335
Size: 32 135 32 638 32 34 32 27
Cuts at: 2335 2367 3038 3070 3196 3228 3839 3871 3874
Size: 32 671 32 126 32 611 32 3
Cuts at: 3874 3906 4310 4342 5770 5802 5844 5876 1373
Size: 32 404 32 1428 32 42 32 2626
Fragments arranged by size:
2626 1428 671 638 611 404 135 126
42 34 32 32 32 32 32 32
32 32 32 32 32 32 27 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2874 3789 4473 4719 669
Size: 988 281 936 915 684 246 3079
Fragments arranged by size:
3079 988 936 915 684 281 246
HindIII A'AGCT_T
Cuts at: 748 4290 748
Size: 3542 3587
HpaI GTT'AAC
Cuts at: 3789 4719 3789
Size: 930 6199
KpnI G_GTAC'C
Cuts at: 4454 4454
Size: 7129
MfeI C'AATT_G
Cuts at: 4728 4728
Size: 7129
MluI A'CGCG_T
Cuts at: 7005 7005
Size: 7129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2800 2826 3215 3252 3939 6243 6427 1305
Size: 1495 26 389 37 687 2304 184 2007
Fragments arranged by size:
2304 2007 1495 687 389 184 37 26
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5307
Fragments arranged by size:
5307 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2228 3342 5087 5446 5605 518
Size: 648 1062 1114 1745 359 159 2042
Fragments arranged by size:
2042 1745 1114 1062 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2364 2620 3449 4004 4545 5222 6370
Size: 412 346 256 829 555 541 677 1148
Cuts at: 6370 6615 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 829 677 555 541 412 346
256 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6577
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7129
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7129
NotI GC'GGCC_GC
Cuts at: 2019 4576 2019
Size: 2557 4572
NruI TCG'CGA
Cuts at: 2118 2118
Size: 7129
NspI r_CATG'y
Cuts at: 1132 1229 2227 2305 2467 4487 6032 1132
Size: 97 998 78 162 2020 1545 2229
Fragments arranged by size:
2229 2020 1545 998 162 97 78
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7129
PciI A'CATG_T
Cuts at: 6028 6028
Size: 7129
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3234 1108
Size: 2126 5003
PmeI GTTT'AAAC
Cuts at: 4433 4433
Size: 7129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5198 5230 6711 6743 5198
Size: 32 1481 32 5584
Fragments arranged by size:
5584 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2831 2886 3382 2831
Size: 55 496 6578
Fragments arranged by size:
6578 496 55
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7129
PsiI TTA'TAA
Cuts at: 4699 4699
Size: 7129
PstI C_TGCA'G
Cuts at: 830 2320 4153 4481 830
Size: 1490 1833 328 3478
Fragments arranged by size:
3478 1833 1490 328
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7129
PvuII CAG'CTG
Cuts at: 2620 3449 4004 2620
Size: 829 555 5745
Fragments arranged by size:
5745 829 555
SacII CC_GC'GG
Cuts at: 2019 2019
Size: 7129
SalI G'TCGA_C
Cuts at: 1936 2872 4471 1936
Size: 936 1599 4594
Fragments arranged by size:
4594 1599 936
SanDI GG'GwC_CC
Cuts at: 2886 2886
Size: 7129
SapI GCTCTTCn'nnn_
Cuts at: 2890 3294 3822 2890
Size: 404 528 6197
Fragments arranged by size:
6197 528 404
SbfI CC_TGCA'GG
Cuts at: 4481 4481
Size: 7129
ScaI AGT'ACT
Cuts at: 5377 7089 5377
Size: 1712 5417
SexAI A'CCwGG_T
Cuts at: 2574 2574
Size: 7129
SfcI C'TryA_G
Cuts at: 826 1045 2316 4149 4477 5612 6293 6484 826
Size: 219 1271 1833 328 1135 681 191 1471
Fragments arranged by size:
1833 1471 1271 1135 681 328 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7129
SmaI CCC'GGG
Cuts at: 1872 4456 1872
Size: 2584 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2747 2867 3131 3218 5233 6134
Size: 197 926 804 120 264 87 2015 901
Cuts at: 6134 6396 820
Size: 262 1553
Fragments arranged by size:
2015 1553 926 901 804 264 262 197
120 87
SnaBI TAC'GTA
Cuts at: 493 4920 493
Size: 4427 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7129
SphI G_CATG'C
Cuts at: 1132 4487 1132
Size: 3355 3774
SspI AAT'ATT
Cuts at: 5 52 5053 5
Size: 47 5001 2081
Fragments arranged by size:
5001 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7129
StyI C'CwwG_G
Cuts at: 513 1065 2827 2890 3079 3456 4568 513
Size: 552 1762 63 189 377 1112 3074
Fragments arranged by size:
3074 1762 1112 552 377 189 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2327 3176 5156 5173 5326 5511 5956
Size: 1255 747 849 1980 17 153 185 445
Cuts at: 5956 325
Size: 1498
Fragments arranged by size:
1980 1498 1255 849 747 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3160 3476 3611
Size: 274 80 33 51 1443 1183 316 135
Cuts at: 3611 3991 4241 5375 7087 96
Size: 380 250 1134 1712 138
Fragments arranged by size:
1712 1443 1183 1134 380 316 274 250
138 135 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7089 7121 7089
Size: 32 7097
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7129
XbaI T'CTAG_A
Cuts at: 4465 4465
Size: 7129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3231 3231
Size: 7129
XhoI C'TCGA_G
Cuts at: 1943 2747 1943
Size: 804 6325
XmaI C'CCGG_G
Cuts at: 1870 4454 1870
Size: 2584 4545
XmnI GAAnn'nnTTC
Cuts at: 3114 3428 5258 3114
Size: 314 1830 4985
Fragments arranged by size:
4985 1830 314
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4937 276
Size: 53 83 186 936 3403 2468
Fragments arranged by size:
3403 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BbeI BfrBI BsiWI BsmBI DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SfiI SfoI SrfI SwaI