Restriction map for
pFN21AB6293
(Circular) (Six-base) MAPSORT of: pFN21AB6293.seq Check: 8658 from: 1 to: 7123
pFN21AB6293 7123 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2497 4351 2497
Size: 1854 5269
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2589 2655 4933 278
Size: 53 83 186 936 1053 66 2278 2468
Fragments arranged by size:
2468 2278 1053 936 186 83 66 53
AccI GT'mk_AC
Cuts at: 1937 4466 1937
Size: 2529 4594
Acc65I G'GTAC_C
Cuts at: 4444 4444
Size: 7123
AclI AA'CG_TT
Cuts at: 1563 5250 5623 1563
Size: 3687 373 3063
Fragments arranged by size:
3687 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5186 6570 6838 1632
Size: 3554 1384 268 1917
Fragments arranged by size:
3554 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6926
AflIII A'CryG_T
Cuts at: 1203 2418 4269 6022 6999 1203
Size: 1215 1851 1753 977 1327
Fragments arranged by size:
1851 1753 1327 1215 977
AgeI A'CCGG_T
Cuts at: 5954 5954
Size: 7123
AhdI GACnn_n'nnGTC
Cuts at: 2098 3625 5852 2098
Size: 1527 2227 3369
Fragments arranged by size:
3369 2227 1527
AleI CACnn'nnGTG
Cuts at: 1166 4253 1166
Size: 3087 4036
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7123
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4190 4220 4222 4252 4190
Size: 30 2 30 7061
Fragments arranged by size:
7061 30 30 2
AlwNI CAG_nnn'CTG
Cuts at: 2146 2176 2257 2692 2979 4231 4375 4866 6438
Size: 30 81 435 287 1252 144 491 1572
Cuts at: 6438 2146
Size: 2831
Fragments arranged by size:
2831 1572 1252 491 435 287 144 81
30
ApaLI G'TGCA_C
Cuts at: 5180 6336 5180
Size: 1156 5967
ApoI r'AATT_y
Cuts at: 1493 3463 4432 4658 4810 6919 1493
Size: 1970 969 226 152 2109 1697
Fragments arranged by size:
2109 1970 1697 969 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5677 160
Size: 872 4645 1606
Fragments arranged by size:
4645 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7123
AvaI C'yCGr_G
Cuts at: 1870 1943 2472 2538 3307 3740 4009 4448 1870
Size: 73 529 66 769 433 269 439 4545
Fragments arranged by size:
4545 769 529 439 433 269 73 66
BamHI G'GATC_C
Cuts at: 2049 3312 4453 4820 2049
Size: 1263 1141 367 4352
Fragments arranged by size:
4352 1263 1141 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3323 4444 5899 618
Size: 325 217 627 1536 1121 1455 1842
Fragments arranged by size:
1842 1536 1455 1121 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2313 2550 2906 4062 4442 1391
Size: 922 237 356 1156 380 4072
Fragments arranged by size:
4072 1156 922 380 356 237
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2866 928
Size: 971 967 5185
Fragments arranged by size:
5185 971 967
BbvCI CC'TCA_GC
Cuts at: 2030 2126 3089 3693 2030
Size: 96 963 604 5460
Fragments arranged by size:
5460 963 604 96
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5314 5348 1903
Size: 34 3377 34 3678
Fragments arranged by size:
3678 3377 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3873 5015 6225 3873
Size: 1142 1210 4771
Fragments arranged by size:
4771 1210 1142
BclI T'GATC_A
Cuts at: 1548 3535 1548
Size: 1987 5136
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5734 136
Size: 107 122 71 631 605 4062 1525
Fragments arranged by size:
4062 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2769 3496 7118 2769
Size: 727 3622 2774
Fragments arranged by size:
3622 2774 727
BlpI GC'TnA_GC
Cuts at: 4540 4540
Size: 7123
Bme1580I G_kGCm'C
Cuts at: 1790 3326 4306 5184 6340 1790
Size: 1536 980 878 1156 2573
Fragments arranged by size:
2573 1536 1156 980 878
BmgBI CAC'GTC
Cuts at: 1318 2562 2968 3451 1318
Size: 1244 406 483 4990
Fragments arranged by size:
4990 1244 483 406
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3241 4854 5812
Size: 416 53 83 399 24 1824 1613 958
Cuts at: 5812 442
Size: 1753
Fragments arranged by size:
1824 1753 1613 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 7123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2597 2629 2714 2758 5783 1239
Size: 1358 32 85 44 3025 2579
Fragments arranged by size:
3025 2579 1358 85 44 32
Bpu10I CC'TnA_GC
Cuts at: 2030 2126 3089 3693 2030
Size: 96 963 604 5460
Fragments arranged by size:
5460 963 604 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4130 5248 6113 6411 4130
Size: 1118 865 298 4842
Fragments arranged by size:
4842 1118 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2780 5786 882
Size: 617 1281 3006 2219
Fragments arranged by size:
3006 2219 1281 617
BsaAI yAC'GTr
Cuts at: 493 1204 2155 2599 3379 4914 493
Size: 711 951 444 780 1535 2702
Fragments arranged by size:
2702 1535 951 780 711 444
BsaBI GATnn'nnATC
Cuts at: 7117 7117
Size: 7123
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2586 2652 4139 4930
Size: 53 83 186 936 1053 66 1487 791
Cuts at: 4930 5312 5947 275
Size: 382 635 1451
Fragments arranged by size:
1487 1451 1053 936 791 635 382 186
83 66 53
BsaWI w'CCGG_w
Cuts at: 3831 5556 5954 6228 6375 6786 3831
Size: 1725 398 274 147 411 4168
Fragments arranged by size:
4168 1725 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2309 2339 2348 2378 4190
Size: 30 165 30 906 30 9 30 1812
Cuts at: 4190 4220 4250 1178
Size: 30 30 4051
Fragments arranged by size:
4051 1812 906 165 30 30 30 30
30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2594 3941 3944 3947 4214 1186
Size: 949 459 1347 3 3 267 4095
Fragments arranged by size:
4095 1347 949 459 267 3 3
BseYI C'CCAG_C
Cuts at: 2824 3101 3844 4077 6326 6822 2824
Size: 277 743 233 2249 496 3125
Fragments arranged by size:
3125 2249 743 496 277 233
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6550
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2385 2891 3397 3629 4157 4254
Size: 275 186 384 506 506 232 528 97
Cuts at: 4254 4573 5334 6362 1540
Size: 319 761 1028 2301
Fragments arranged by size:
2301 1028 761 528 506 506 384 319
275 232 186 97
BsiHKAI G_wGCw'C
Cuts at: 2280 2313 5184 5269 6340 2280
Size: 33 2871 85 1071 3063
Fragments arranged by size:
3063 2871 1071 85 33
BsmI GAATG_Cn'
Cuts at: 4634 4727 4634
Size: 93 7030
BsmBI CGTCTCn'nnnn_
Cuts at: 2423 2920 4196 2423
Size: 497 1276 5350
Fragments arranged by size:
5350 1276 497
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2280 2313 2550 2906 3326 4062 4306
Size: 399 490 33 237 356 420 736 244
Cuts at: 4306 4442 5184 5269 6340 1391
Size: 136 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 736 490 420 399 356
244 237 136 85 33
BspEI T'CCGG_A
Cuts at: 6786 6786
Size: 7123
BspHI T'CATG_A
Cuts at: 5012 6742 5012
Size: 1730 5393
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2497 4351 4478 844
Size: 1653 1854 127 3489
Fragments arranged by size:
3489 1854 1653 127
BsrBI CCG'CTC
Cuts at: 1394 1823 2299 2746 2887 2937 3169 4164 5010
Size: 429 476 447 141 50 232 995 846
Cuts at: 5010 1394
Size: 3507
Fragments arranged by size:
3507 995 846 476 447 429 232 141
50
BsrDI GCAATG_nn'
Cuts at: 65 1248 3661 4420 5618 5792 65
Size: 1183 2413 759 1198 174 1396
Fragments arranged by size:
2413 1396 1198 1183 759 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2569 3012 3637 4152 4156 5767 5954
Size: 110 759 443 625 515 4 1611 187
Cuts at: 5954 1700
Size: 2869
Fragments arranged by size:
2869 1611 759 625 515 443 187 110
4
BsrGI T'GTAC_A
Cuts at: 96 2445 96
Size: 2349 4774
BssHII G'CGCG_C
Cuts at: 1925 2660 2662 1925
Size: 735 2 6386
Fragments arranged by size:
6386 735 2
BssSI C'ACGA_G
Cuts at: 2273 3764 5183 6195 2273
Size: 1491 1419 1012 3201
Fragments arranged by size:
3201 1491 1419 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2955 4281 794
Size: 2161 1326 3636
Fragments arranged by size:
3636 2161 1326
BstBI TT'CG_AA
Cuts at: 5927 5927
Size: 7123
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7123
BstXI CCAn_nnnn'nTGG
Cuts at: 3151 3383 3151
Size: 232 6891
BstYI r'GATC_y
Cuts at: 1972 2049 2136 2769 3312 3496 4292 4453 4820
Size: 77 87 633 543 184 796 161 367
Cuts at: 4820 5205 5222 6674 6760 6772 7118 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 796 633 543 385 367 346
184 161 87 86 77 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2064 2371 2907 3318 3457 3519
Size: 552 939 60 307 536 411 139 62
Cuts at: 3519 513
Size: 4117
Fragments arranged by size:
4117 939 552 536 411 307 139 62
60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3057 487
Size: 683 56 1831 4553
Fragments arranged by size:
4553 1831 683 56
BtsI GCAGTG_nn'
Cuts at: 2953 3100 4639 5433 5453 2953
Size: 147 1539 794 20 4623
Fragments arranged by size:
4623 1539 794 147 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7123
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7088
DraI TTT'AAA
Cuts at: 4427 4427
Size: 7123
DraIII CAC_nnn'GTG
Cuts at: 2046 2089 3330 4270 2046
Size: 43 1241 940 4899
Fragments arranged by size:
4899 1241 940 43
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2228 6130 809
Size: 507 912 3902 1802
Fragments arranged by size:
3902 1802 912 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2206 2382 2888
Size: 54 1425 50 275 18 376 176 506
Cuts at: 2888 3207 3394 3888 4154 4417 4570 5459 7088
Size: 319 187 494 266 263 153 889 1629
Cuts at: 7088 8
Size: 43
Fragments arranged by size:
1629 1425 889 506 494 376 319 275
266 263 187 176 153 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2382 2888 3394 4154 4570 1537
Size: 275 570 506 506 760 416 4090
Fragments arranged by size:
4090 760 570 506 506 416 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2222 2927 4403 5053 1353
Size: 869 705 1476 650 3423
Fragments arranged by size:
3423 1476 869 705 650
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2146 2222 2252 5710 6084 6230
Size: 1064 138 557 76 30 3458 374 146
Cuts at: 6230 387
Size: 1280
Fragments arranged by size:
3458 1280 1064 557 374 146 138 76
30
EcoICRI GAG'CTC
Cuts at: 2311 2311
Size: 7123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2597 2629 2714 2758 5186 5783 6570
Size: 393 965 32 85 44 2428 597 787
Cuts at: 6570 6838 1239
Size: 268 1524
Fragments arranged by size:
2428 1524 965 787 597 393 268 85
44 32
EcoO109I rG'GnC_Cy
Cuts at: 2530 2689 3019 4023 4132 2530
Size: 159 330 1004 109 5521
Fragments arranged by size:
5521 1004 330 159 109
EcoRI G'AATT_C
Cuts at: 4432 4810 4432
Size: 378 6745
FspI TGC'GCA
Cuts at: 3653 5629 3653
Size: 1976 5147
HaeII r_GCGC'y
Cuts at: 1678 2576 3566 3775 6270 1678
Size: 898 990 209 2495 2531
Fragments arranged by size:
2531 2495 990 898 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2116 2309 2341 2348
Size: 32 135 32 512 32 193 32 7
Cuts at: 2348 2380 3333 3365 3611 3643 5764 5796 5838
Size: 32 953 32 246 32 2121 32 42
Cuts at: 5838 5870 1373
Size: 32 2626
Fragments arranged by size:
2626 2121 953 512 246 193 135 42
32 32 32 32 32 32 32 32
32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2914 4467 4713 669
Size: 988 281 976 1553 246 3079
Fragments arranged by size:
3079 1553 988 976 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7123
HpaI GTT'AAC
Cuts at: 4713 4713
Size: 7123
KpnI G_GTAC'C
Cuts at: 4448 4448
Size: 7123
MfeI C'AATT_G
Cuts at: 4722 4722
Size: 7123
MluI A'CGCG_T
Cuts at: 6999 6999
Size: 7123
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2061 2340 2831 3312 6237 6421 1305
Size: 756 279 491 481 2925 184 2007
Fragments arranged by size:
2925 2007 756 491 481 279 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3890 10
Size: 54 1425 343 2058 3243
Fragments arranged by size:
3243 2058 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2483 2861 4253 4361 5081 5440 5599
Size: 648 1317 378 1392 108 720 359 159
Cuts at: 5599 518
Size: 2042
Fragments arranged by size:
2042 1392 1317 720 648 378 359 159
108
MspA1I CmG'CkG
Cuts at: 1606 3226 3521 3692 4317 4539 5216 6364 6609
Size: 1620 295 171 625 222 677 1148 245
Cuts at: 6609 1606
Size: 2120
Fragments arranged by size:
2120 1620 1148 677 625 295 245 222
171
NaeI GCC'GGC
Cuts at: 2571 3014 4158 2571
Size: 443 1144 5536
Fragments arranged by size:
5536 1144 443
NcoI C'CATG_G
Cuts at: 513 1065 2004 2064 2907 3457 513
Size: 552 939 60 843 550 4179
Fragments arranged by size:
4179 939 843 552 550 60
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7123
NgoMIV G'CCGG_C
Cuts at: 2569 3012 4156 2569
Size: 443 1144 5536
Fragments arranged by size:
5536 1144 443
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7123
NotI GC'GGCC_GC
Cuts at: 2382 4570 2382
Size: 2188 4935
NspI r_CATG'y
Cuts at: 1132 1229 2422 4481 6026 1132
Size: 97 1193 2059 1545 2229
Fragments arranged by size:
2229 2059 1545 1193 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7123
PciI A'CATG_T
Cuts at: 2418 6022 2418
Size: 3604 3519
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2089 2257 3621 4122 1108
Size: 981 168 1364 501 4109
Fragments arranged by size:
4109 1364 981 501 168
PfoI T'CCnGG_A
Cuts at: 2139 2774 2139
Size: 635 6488
PmeI GTTT'AAAC
Cuts at: 4427 4427
Size: 7123
PmlI CAC'GTG
Cuts at: 2155 2599 3379 2155
Size: 444 780 5899
Fragments arranged by size:
5899 780 444
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4220 4222 4252 5192 5224 6705 6737 4190
Size: 30 2 30 940 32 1481 32 4576
Fragments arranged by size:
4576 1481 940 32 32 30 30 2
PpuMI rG'GwC_Cy
Cuts at: 2689 3019 4023 4132 2689
Size: 330 1004 109 5680
Fragments arranged by size:
5680 1004 330 109
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7123
PsiI TTA'TAA
Cuts at: 4693 4693
Size: 7123
PstI C_TGCA'G
Cuts at: 830 2172 2555 4380 4475 830
Size: 1342 383 1825 95 3478
Fragments arranged by size:
3478 1825 1342 383 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7123
PvuII CAG'CTG
Cuts at: 4317 4317
Size: 7123
SacI G_AGCT'C
Cuts at: 2313 2313
Size: 7123
SacII CC_GC'GG
Cuts at: 3522 3522
Size: 7123
SalI G'TCGA_C
Cuts at: 1936 4465 1936
Size: 2529 4594
SanDI GG'GwC_CC
Cuts at: 4023 4023
Size: 7123
SapI GCTCTTCn'nnn_
Cuts at: 2927 4403 2927
Size: 1476 5647
SbfI CC_TGCA'GG
Cuts at: 4475 4475
Size: 7123
ScaI AGT'ACT
Cuts at: 5371 7083 5371
Size: 1712 5411
SfcI C'TryA_G
Cuts at: 826 1045 2168 2182 2551 4063 4376 4471 5606
Size: 219 1123 14 369 1512 313 95 1135
Cuts at: 5606 6287 6478 826
Size: 681 191 1471
Fragments arranged by size:
1512 1471 1135 1123 681 369 313 219
191 95 14
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7123
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7123
SmaI CCC'GGG
Cuts at: 1872 2540 3309 4450 1872
Size: 668 769 1141 4545
Fragments arranged by size:
4545 1141 769 668
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4145 5227 6128 6390 820
Size: 197 926 2202 1082 901 262 1553
Fragments arranged by size:
2202 1553 1082 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4914 493
Size: 4421 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7123
SphI G_CATG'C
Cuts at: 1132 4481 1132
Size: 3349 3774
SspI AAT'ATT
Cuts at: 5 52 5047 5
Size: 47 4995 2081
Fragments arranged by size:
4995 2081 47
StuI AGG'CCT
Cuts at: 1517 2166 1517
Size: 649 6474
StyI C'CwwG_G
Cuts at: 513 1065 2004 2064 2112 2292 2739 2907 3264
Size: 552 939 60 48 180 447 168 357
Cuts at: 3264 3457 3615 3809 4050 4562 513
Size: 193 158 194 241 512 3074
Fragments arranged by size:
3074 939 552 512 447 357 241 194
193 180 168 158 60 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2384 5150 5167 5320 5505 5950 325
Size: 1255 804 2766 17 153 185 445 1498
Fragments arranged by size:
2766 1498 1255 804 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2445 5369 7081
Size: 274 80 33 51 1443 468 2924 1712
Cuts at: 7081 96
Size: 138
Fragments arranged by size:
2924 1712 1443 468 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7083 7115 7083
Size: 32 7091
Tth111I GACn'n_nGTC
Cuts at: 1316 2870 1316
Size: 1554 5569
XbaI T'CTAG_A
Cuts at: 4459 4459
Size: 7123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2254 2572 2821 3562 2254
Size: 318 249 741 5815
Fragments arranged by size:
5815 741 318 249
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7123
XmaI C'CCGG_G
Cuts at: 1870 2538 3307 4448 1870
Size: 668 769 1141 4545
Fragments arranged by size:
4545 1141 769 668
XmnI GAAnn'nnTTC
Cuts at: 3467 5252 3467
Size: 1785 5338
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2587 2653 4931 276
Size: 53 83 186 936 1053 66 2278 2468
Fragments arranged by size:
2468 2278 1053 936 186 83 66 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII
SexAI SfiI SfoI SrfI SwaI