Restriction map for
pFN21AB6292
(Circular) (Six-base) MAPSORT of: pFN21AB6292.seq Check: 3852 from: 1 to: 7072
pFN21AB6292 7072 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3756 3852 3756
Size: 96 6976
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4882 278
Size: 53 83 186 936 3346 2468
Fragments arranged by size:
3346 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3234 4415 1937
Size: 1297 1181 4594
Fragments arranged by size:
4594 1297 1181
Acc65I G'GTAC_C
Cuts at: 4327 4393 4327
Size: 66 7006
AclI AA'CG_TT
Cuts at: 1563 3630 5199 5572 1563
Size: 2067 1569 373 3063
Fragments arranged by size:
3063 2067 1569 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3614 3686 5135 6519 6787 1632
Size: 1982 72 1449 1384 268 1917
Fragments arranged by size:
1982 1917 1449 1384 268 72
AfeI AGC'GCT
Cuts at: 3175 3175
Size: 7072
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6875
AflIII A'CryG_T
Cuts at: 1203 2228 3030 3475 5971 6948 1203
Size: 1025 802 445 2496 977 1327
Fragments arranged by size:
2496 1327 1025 977 802 445
AgeI A'CCGG_T
Cuts at: 5903 5903
Size: 7072
AhdI GACnn_n'nnGTC
Cuts at: 5801 5801
Size: 7072
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7072
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2387 1184
Size: 1203 5869
AlwNI CAG_nnn'CTG
Cuts at: 2051 4815 6387 2051
Size: 2764 1572 2736
Fragments arranged by size:
2764 2736 1572
ApaI G_GGCC'C
Cuts at: 2806 2990 2806
Size: 184 6888
ApaLI G'TGCA_C
Cuts at: 3683 3911 5129 6285 3683
Size: 228 1218 1156 4470
Fragments arranged by size:
4470 1218 1156 228
ApoI r'AATT_y
Cuts at: 1493 4170 4381 4607 4759 6868 1493
Size: 2677 211 226 152 2109 1697
Fragments arranged by size:
2677 2109 1697 226 211 152
AseI AT'TA_AT
Cuts at: 160 1032 5626 160
Size: 872 4594 1606
Fragments arranged by size:
4594 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7072
AvaI C'yCGr_G
Cuts at: 1870 1943 2400 2781 3217 3295 3942 4121 4397
Size: 73 457 381 436 78 647 179 276
Cuts at: 4397 1870
Size: 4545
Fragments arranged by size:
4545 647 457 436 381 276 179 78
73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2109 2142 2109
Size: 33 7039
BamHI G'GATC_C
Cuts at: 4366 4402 4769 4366
Size: 36 367 6669
Fragments arranged by size:
6669 367 36
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3367 4039 4327 4393 5848
Size: 325 217 627 1580 672 288 66 1455
Cuts at: 5848 618
Size: 1842
Fragments arranged by size:
1842 1580 1455 672 627 325 288 217
66
BanII G_rGCy'C
Cuts at: 1391 2762 2806 2945 2990 3059 3302 3322 4391
Size: 1371 44 139 45 69 243 20 1069
Cuts at: 4391 1391
Size: 4072
Fragments arranged by size:
4072 1371 1069 243 139 69 45 44
20
BbeI G_GCGC'C
Cuts at: 3371 4043 3371
Size: 672 6400
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2755 2823 928
Size: 971 856 68 5177
Fragments arranged by size:
5177 971 856 68
BbvCI CC'TCA_GC
Cuts at: 3499 3499
Size: 7072
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3229 3263 3286 3320 5263 5297 1903
Size: 34 1292 34 23 34 1943 34 3678
Fragments arranged by size:
3678 1943 1292 34 34 34 34 23
BciVI GTATCCnnnnn_n'
Cuts at: 4964 6174 4964
Size: 1210 5862
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7072
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3467 5683 136
Size: 107 122 71 631 605 1795 2216 1525
Fragments arranged by size:
2216 1795 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4060 4282 7067 4060
Size: 222 2785 4065
Fragments arranged by size:
4065 2785 222
BlpI GC'TnA_GC
Cuts at: 4489 4489
Size: 7072
Bme1580I G_kGCm'C
Cuts at: 1790 2228 2806 2990 3230 3687 3890 3915 5133
Size: 438 578 184 240 457 203 25 1218
Cuts at: 5133 6289 1790
Size: 1156 2573
Fragments arranged by size:
2573 1218 1156 578 457 438 240 203
184 25
BmgBI CAC'GTC
Cuts at: 1318 2753 4129 1318
Size: 1435 1376 4261
Fragments arranged by size:
4261 1435 1376
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3898 3961 4803
Size: 416 53 83 399 24 2481 63 842
Cuts at: 4803 5761 442
Size: 958 1753
Fragments arranged by size:
2481 1753 958 842 416 399 83 63
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7072
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2929 3317 3879 5732 1239
Size: 1690 388 562 1853 2579
Fragments arranged by size:
2579 1853 1690 562 388
Bpu10I CC'TnA_GC
Cuts at: 3170 3499 3170
Size: 329 6743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2921 2982 3818 3986 5197 6062 6360 2921
Size: 61 836 168 1211 865 298 3633
Fragments arranged by size:
3633 1211 865 836 298 168 61
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2848 3447 5735 882
Size: 617 1349 599 2288 2219
Fragments arranged by size:
2288 2219 1349 617 599
BsaAI yAC'GTr
Cuts at: 493 1204 2862 4863 493
Size: 711 1658 2001 2702
Fragments arranged by size:
2702 2001 1658 711
BsaBI GATnn'nnATC
Cuts at: 2438 7066 2438
Size: 4628 2444
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3368 4040 4879 5261
Size: 53 83 186 936 1835 672 839 382
Cuts at: 5261 5896 275
Size: 635 1451
Fragments arranged by size:
1835 1451 936 839 672 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5505 5903 6177 6324 6735 5505
Size: 398 274 147 411 5842
Fragments arranged by size:
5842 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2996 3026 3947 3977 1178
Size: 30 165 30 1593 30 921 30 4273
Fragments arranged by size:
4273 1593 921 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3236 4238 4243 1186
Size: 2050 1002 5 4015
Fragments arranged by size:
4015 2050 1002 5
BseYI C'CCAG_C
Cuts at: 3518 4307 6275 6771 3518
Size: 789 1968 496 3819
Fragments arranged by size:
3819 1968 789 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3503 3908 3920 4282 1163
Size: 573 1767 405 12 362 3953
Fragments arranged by size:
3953 1767 573 405 362 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4522 5283 6311 1540
Size: 275 186 2521 761 1028 2301
Fragments arranged by size:
2521 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2594 2945 3006 3302 3687 3915 3965 5133 5218
Size: 351 61 296 385 228 50 1168 85
Cuts at: 5218 6289 2594
Size: 1071 3377
Fragments arranged by size:
3377 1168 1071 385 351 296 228 85
61 50
BsmI GAATG_Cn'
Cuts at: 3629 3797 4583 4676 3629
Size: 168 786 93 6025
Fragments arranged by size:
6025 786 168 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2228 2594 2762 2806 2945 2990 3006
Size: 399 438 366 168 44 139 45 16
Cuts at: 3006 3059 3230 3302 3322 3687 3890 3915 3965
Size: 53 171 72 20 365 203 25 50
Cuts at: 3965 4391 5133 5218 6289 1391
Size: 426 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 438 426 399 366 365
203 171 168 139 85 72 53 50
45 44 25 20 16
BspEI T'CCGG_A
Cuts at: 6735 6735
Size: 7072
BspHI T'CATG_A
Cuts at: 4293 4961 6691 4293
Size: 668 1730 4674
Fragments arranged by size:
4674 1730 668
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3756 3852 4427 844
Size: 2912 96 575 3489
Fragments arranged by size:
3489 2912 575 96
BsrBI CCG'CTC
Cuts at: 1394 1823 3949 4959 1394
Size: 429 2126 1010 3507
Fragments arranged by size:
3507 2126 1010 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5567 5741 65
Size: 1183 4319 174 1396
Fragments arranged by size:
4319 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3566 4116 5716 5903 1700
Size: 110 1756 550 1600 187 2869
Fragments arranged by size:
2869 1756 1600 550 187 110
BsrGI T'GTAC_A
Cuts at: 96 2069 96
Size: 1973 5099
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7072
BssSI C'ACGA_G
Cuts at: 5132 6144 5132
Size: 1012 6060
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3633 3885 794
Size: 2839 252 3981
Fragments arranged by size:
3981 2839 252
BstBI TT'CG_AA
Cuts at: 5876 5876
Size: 7072
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7072
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 7072
BstYI r'GATC_y
Cuts at: 1972 2307 4060 4282 4366 4402 4769 5154 5171
Size: 335 1753 222 84 36 367 385 17
Cuts at: 5171 6623 6709 6721 7067 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1753 1452 385 367 346 335 222
86 84 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 4255 4255
Size: 7072
BtgI C'CryG_G
Cuts at: 513 1065 3447 3465 4179 513
Size: 552 2382 18 714 3406
Fragments arranged by size:
3406 2382 714 552 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3521 487
Size: 683 56 2295 4038
Fragments arranged by size:
4038 2295 683 56
BtsI GCAGTG_nn'
Cuts at: 4588 5382 5402 4588
Size: 794 20 6258
Fragments arranged by size:
6258 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7072
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7037
DraI TTT'AAA
Cuts at: 4376 4376
Size: 7072
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6079 809
Size: 507 4763 1802
Fragments arranged by size:
4763 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3649 4176 4519
Size: 54 1425 50 275 18 1819 527 343
Cuts at: 4519 5408 7037 8
Size: 889 1629 43
Fragments arranged by size:
1819 1629 1425 889 527 343 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4519 1537
Size: 275 2707 4090
Fragments arranged by size:
4090 2707 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2055 2178 2349 3218 5002 1353
Size: 702 123 171 869 1784 3423
Fragments arranged by size:
3423 1784 869 702 171 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2147 5659 6033 6179 387
Size: 1064 138 558 3512 374 146 1280
Fragments arranged by size:
3512 1280 1064 558 374 146 138
EcoICRI GAG'CTC
Cuts at: 2943 3300 2943
Size: 357 6715
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2929 3317 3614 3686 3879 5135 5732
Size: 393 1297 388 297 72 193 1256 597
Cuts at: 5732 6519 6787 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1297 1256 787 597 393 388 297
268 193 72
EcoNI CCTnn'n_nnAGG
Cuts at: 2146 2146
Size: 7072
EcoO109I rG'GnC_Cy
Cuts at: 2689 2731 2803 2987 2689
Size: 42 72 184 6774
Fragments arranged by size:
6774 184 72 42
EcoRI G'AATT_C
Cuts at: 4381 4759 4381
Size: 378 6694
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3154 3154
Size: 7072
FspI TGC'GCA
Cuts at: 5578 5578
Size: 7072
HaeII r_GCGC'y
Cuts at: 1678 3177 3371 4043 6219 1678
Size: 1499 194 672 2176 2531
Fragments arranged by size:
2531 2176 1499 672 194
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5713 5745 5787 5819 1373
Size: 32 135 32 4141 32 42 32 2626
Fragments arranged by size:
4141 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4416 4662 669
Size: 988 281 2478 246 3079
Fragments arranged by size:
3079 2478 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2829 748
Size: 2081 4991
HpaI GTT'AAC
Cuts at: 4662 4662
Size: 7072
KasI G'GCGC_C
Cuts at: 3367 4039 3367
Size: 672 6400
KpnI G_GTAC'C
Cuts at: 4331 4397 4331
Size: 66 7006
MfeI C'AATT_G
Cuts at: 4671 4671
Size: 7072
MluI A'CGCG_T
Cuts at: 3030 6948 3030
Size: 3918 3154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2694 6186 6370 1305
Size: 1389 3492 184 2007
Fragments arranged by size:
3492 2007 1389 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5250
Fragments arranged by size:
5250 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3790 3874 4145 5030 5389 5548
Size: 648 843 1781 84 271 885 359 159
Cuts at: 5548 518
Size: 2042
Fragments arranged by size:
2042 1781 885 843 648 359 271 159
84
MspA1I CmG'CkG
Cuts at: 1606 3592 4488 5165 6313 6558 1606
Size: 1986 896 677 1148 245 2120
Fragments arranged by size:
2120 1986 1148 896 677 245
NarI GG'CG_CC
Cuts at: 3368 4040 3368
Size: 672 6400
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6520
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7072
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7072
NotI GC'GGCC_GC
Cuts at: 4519 4519
Size: 7072
NspI r_CATG'y
Cuts at: 1132 1229 3479 4430 5975 1132
Size: 97 2250 951 1545 2229
Fragments arranged by size:
2250 2229 1545 951 97
PasI CC'CwG_GG
Cuts at: 1348 2094 2963 3348 1348
Size: 746 869 385 5072
Fragments arranged by size:
5072 869 746 385
PciI A'CATG_T
Cuts at: 3475 5971 3475
Size: 2496 4576
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7072
PfoI T'CCnGG_A
Cuts at: 2533 2533
Size: 7072
PmeI GTTT'AAAC
Cuts at: 4376 4376
Size: 7072
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5141 5173 6654 6686 5141
Size: 32 1481 32 5527
Fragments arranged by size:
5527 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2689 2731 2689
Size: 42 7030
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7072
PsiI TTA'TAA
Cuts at: 4642 4642
Size: 7072
PspOMI G'GGCC_C
Cuts at: 2802 2986 2802
Size: 184 6888
PspXI vC'TCGA_Gb
Cuts at: 4121 4121
Size: 7072
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3020 3052 3020
Size: 32 7040
PstI C_TGCA'G
Cuts at: 830 3753 4424 830
Size: 2923 671 3478
Fragments arranged by size:
3478 2923 671
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7072
SacI G_AGCT'C
Cuts at: 2945 3302 2945
Size: 357 6715
SalI G'TCGA_C
Cuts at: 1936 4414 1936
Size: 2478 4594
SbfI CC_TGCA'GG
Cuts at: 4424 4424
Size: 7072
ScaI AGT'ACT
Cuts at: 5320 7032 5320
Size: 1712 5360
SexAI A'CCwGG_T
Cuts at: 3762 3762
Size: 7072
SfcI C'TryA_G
Cuts at: 826 1045 3235 3424 3749 3994 4155 4420 5555
Size: 219 2190 189 325 245 161 265 1135
Cuts at: 5555 6236 6427 826
Size: 681 191 1471
Fragments arranged by size:
2190 1471 1135 681 325 265 245 219
191 189 161
SfoI GGC'GCC
Cuts at: 3369 4041 3369
Size: 672 6400
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7072
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7072
SmaI CCC'GGG
Cuts at: 1872 2402 3944 4399 1872
Size: 530 1542 455 4545
Fragments arranged by size:
4545 1542 530 455
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2936 2997 3833 4001 4121 5176
Size: 197 926 993 61 836 168 120 1055
Cuts at: 5176 6077 6339 820
Size: 901 262 1553
Fragments arranged by size:
1553 1055 993 926 901 836 262 197
168 120 61
SnaBI TAC'GTA
Cuts at: 493 4863 493
Size: 4370 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7072
SphI G_CATG'C
Cuts at: 1132 4430 1132
Size: 3298 3774
SspI AAT'ATT
Cuts at: 5 52 2297 4996 5
Size: 47 2245 2699 2081
Fragments arranged by size:
2699 2245 2081 47
StuI AGG'CCT
Cuts at: 1517 3422 1517
Size: 1905 5167
StyI C'CwwG_G
Cuts at: 513 1065 2147 4053 4511 513
Size: 552 1082 1906 458 3074
Fragments arranged by size:
3074 1906 1082 552 458
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3917 5099 5116 5269 5454 5899 325
Size: 1255 2337 1182 17 153 185 445 1498
Fragments arranged by size:
2337 1498 1255 1182 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2069 2637 5318
Size: 274 80 33 51 1443 92 568 2681
Cuts at: 5318 7030 96
Size: 1712 138
Fragments arranged by size:
2681 1712 1443 568 274 138 92 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2996 3028 3947 3979 7032 7064 2996
Size: 32 919 32 3053 32 3004
Fragments arranged by size:
3053 3004 919 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7072
XbaI T'CTAG_A
Cuts at: 4408 4408
Size: 7072
XhoI C'TCGA_G
Cuts at: 1943 4121 1943
Size: 2178 4894
XmaI C'CCGG_G
Cuts at: 1870 2400 3942 4397 1870
Size: 530 1542 455 4545
Fragments arranged by size:
4545 1542 530 455
XmnI GAAnn'nnTTC
Cuts at: 2517 5201 2517
Size: 2684 4388
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4880 276
Size: 53 83 186 936 3346 2468
Fragments arranged by size:
3346 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI SacI
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BfrBI BplI BsiWI BsmBI BstZ17I
DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PvuII RsrII SacII SanDI SapI SfiI
SrfI SwaI XcmI