Restriction map for
pFN21AB6290
(Circular) (Six-base) MAPSORT of: pFN21AB6290.seq Check: 8348 from: 1 to: 7060
pFN21AB6290 7060 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3734 3827 3939 4306 3734
Size: 93 112 367 6488
Fragments arranged by size:
6488 367 112 93
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4870 278
Size: 53 83 186 936 3334 2468
Fragments arranged by size:
3334 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4403 1937
Size: 2466 4594
Acc65I G'GTAC_C
Cuts at: 4381 4381
Size: 7060
AclI AA'CG_TT
Cuts at: 1563 5187 5560 1563
Size: 3624 373 3063
Fragments arranged by size:
3624 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3308 3606 4209 5123 6507 6775 1632
Size: 1676 298 603 914 1384 268 1917
Fragments arranged by size:
1917 1676 1384 914 603 298 268
AfeI AGC'GCT
Cuts at: 4085 4085
Size: 7060
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6863
AflIII A'CryG_T
Cuts at: 1203 4007 5959 6936 1203
Size: 2804 1952 977 1327
Fragments arranged by size:
2804 1952 1327 977
AgeI A'CCGG_T
Cuts at: 5891 5891
Size: 7060
AhdI GACnn_n'nnGTC
Cuts at: 4105 5789 4105
Size: 1684 5376
AleI CACnn'nnGTG
Cuts at: 1166 4047 1166
Size: 2881 4179
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2221 2589 3835 1184
Size: 1037 368 1246 4409
Fragments arranged by size:
4409 1246 1037 368
AlwNI CAG_nnn'CTG
Cuts at: 2432 3554 4803 6375 2432
Size: 1122 1249 1572 3117
Fragments arranged by size:
3117 1572 1249 1122
ApaI G_GGCC'C
Cuts at: 2255 2587 3378 2255
Size: 332 791 5937
Fragments arranged by size:
5937 791 332
ApaLI G'TGCA_C
Cuts at: 3635 5117 6273 3635
Size: 1482 1156 4422
Fragments arranged by size:
4422 1482 1156
ApoI r'AATT_y
Cuts at: 1493 3198 4058 4369 4595 4747 6856 1493
Size: 1705 860 311 226 152 2109 1697
Fragments arranged by size:
2109 1705 1697 860 311 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4338 5614 160
Size: 872 3306 1276 1606
Fragments arranged by size:
3306 1606 1276 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7060
AvaI C'yCGr_G
Cuts at: 1870 1943 2254 3853 4385 1870
Size: 73 311 1599 532 4545
Fragments arranged by size:
4545 1599 532 311 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2754 2787 2754
Size: 33 7027
BamHI G'GATC_C
Cuts at: 3859 4390 4757 3859
Size: 531 367 6162
Fragments arranged by size:
6162 531 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2102 2312 4381 5836 618
Size: 325 217 627 315 210 2069 1455 1842
Fragments arranged by size:
2069 1842 1455 627 325 315 217 210
BanII G_rGCy'C
Cuts at: 1391 2255 2587 3378 3694 3854 4379 1391
Size: 864 332 791 316 160 525 4072
Fragments arranged by size:
4072 864 791 525 332 316 160
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2403 928
Size: 971 504 5585
Fragments arranged by size:
5585 971 504
BbvCI CC'TCA_GC
Cuts at: 3213 3523 3213
Size: 310 6750
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4339 4373 5251 5285 1903
Size: 34 2402 34 878 34 3678
Fragments arranged by size:
3678 2402 878 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4952 6162 4952
Size: 1210 5850
BclI T'GATC_A
Cuts at: 1548 3682 1548
Size: 2134 4926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2582 3242 4259
Size: 107 122 71 631 605 910 660 1017
Cuts at: 4259 5671 136
Size: 1412 1525
Fragments arranged by size:
1525 1412 1017 910 660 631 605 122
107 71
BglII A'GATC_T
Cuts at: 4324 7055 4324
Size: 2731 4329
BlpI GC'TnA_GC
Cuts at: 3693 4477 3693
Size: 784 6276
Bme1580I G_kGCm'C
Cuts at: 1790 2255 2587 3378 3639 3940 4172 5121 6277
Size: 465 332 791 261 301 232 949 1156
Cuts at: 6277 1790
Size: 2573
Fragments arranged by size:
2573 1156 949 791 465 332 301 261
232
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7060
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2589 4791 5749
Size: 416 53 83 399 24 1172 2202 958
Cuts at: 5749 442
Size: 1753
Fragments arranged by size:
2202 1753 1172 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7060
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2905 2905
Size: 7060
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2172 2520 2684 3153 3224 3293 3302 3575
Size: 933 348 164 469 71 69 9 273
Cuts at: 3575 5720 1239
Size: 2145 2579
Fragments arranged by size:
2579 2145 933 469 348 273 164 71
69 9
Bpu10I CC'TnA_GC
Cuts at: 3213 3454 3523 4080 3213
Size: 241 69 557 6193
Fragments arranged by size:
6193 557 241 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3042 3270 5185 6050 6348 3042
Size: 228 1915 865 298 3754
Fragments arranged by size:
3754 1915 865 298 228
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3707 5723 882
Size: 617 2208 2016 2219
Fragments arranged by size:
2219 2208 2016 617
BsaAI yAC'GTr
Cuts at: 493 1204 4851 493
Size: 711 3647 2702
Fragments arranged by size:
3647 2702 711
BsaBI GATnn'nnATC
Cuts at: 7054 7054
Size: 7060
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4867 5249 5884 275
Size: 53 83 186 936 3334 382 635 1451
Fragments arranged by size:
3334 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3357 5493 5891 6165 6312 6723 3357
Size: 2136 398 274 147 411 3694
Fragments arranged by size:
3694 2136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2821 2851 3222 3252 4204
Size: 30 165 30 1418 30 371 30 952
Cuts at: 4204 4234 1178
Size: 30 4004
Fragments arranged by size:
4004 1418 952 371 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2169 2172 2332 3565 4108 1186
Size: 983 3 160 1233 543 4138
Fragments arranged by size:
4138 1233 983 543 160 3
BseYI C'CCAG_C
Cuts at: 2172 2840 6263 6759 2172
Size: 668 3423 496 2473
Fragments arranged by size:
3423 2473 668 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2612 1163
Size: 573 876 5611
Fragments arranged by size:
5611 876 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3793 4510 5271 6299 1540
Size: 275 186 1792 717 761 1028 2301
Fragments arranged by size:
2301 1792 1028 761 717 275 186
BsiHKAI G_wGCw'C
Cuts at: 3639 3694 5121 5206 6277 3639
Size: 55 1427 85 1071 4422
Fragments arranged by size:
4422 1427 1071 85 55
BsmI GAATG_Cn'
Cuts at: 3565 4571 4664 3565
Size: 1006 93 5961
Fragments arranged by size:
5961 1006 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2255 2587 3378 3639 3694 3854 3940
Size: 399 465 332 791 261 55 160 86
Cuts at: 3940 4172 4379 5121 5206 6277 1391
Size: 232 207 742 85 1071 2174
Fragments arranged by size:
2174 1071 791 742 465 399 332 261
232 207 160 86 85 55
BspEI T'CCGG_A
Cuts at: 3357 6723 3357
Size: 3366 3694
BspHI T'CATG_A
Cuts at: 4089 4949 6679 4089
Size: 860 1730 4470
Fragments arranged by size:
4470 1730 860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3734 3827 3939 4184 4306 4415 844
Size: 2890 93 112 245 122 109 3489
Fragments arranged by size:
3489 2890 245 122 112 109 93
BsrBI CCG'CTC
Cuts at: 1394 1823 2459 4947 1394
Size: 429 636 2488 3507
Fragments arranged by size:
3507 2488 636 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2092 2404 2676 3140 3516 4278 4357
Size: 1183 844 312 272 464 376 762 79
Cuts at: 4357 5555 5729 65
Size: 1198 174 1396
Fragments arranged by size:
1396 1198 1183 844 762 464 376 312
272 174 79
BsrFI r'CCGG_y
Cuts at: 1700 1810 3788 5704 5891 1700
Size: 110 1978 1916 187 2869
Fragments arranged by size:
2869 1978 1916 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7060
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7060
BssSI C'ACGA_G
Cuts at: 5120 6132 5120
Size: 1012 6048
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2563 794
Size: 1769 5291
BstBI TT'CG_AA
Cuts at: 5864 5864
Size: 7060
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7060
BstXI CCAn_nnnn'nTGG
Cuts at: 2885 3010 3131 3713 3902 4246 2885
Size: 125 121 582 189 344 5699
Fragments arranged by size:
5699 582 344 189 125 121
BstYI r'GATC_y
Cuts at: 1972 3859 4324 4390 4757 5142 5159 6611 6697
Size: 1887 465 66 367 385 17 1452 86
Cuts at: 6697 6709 7055 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1887 1452 465 385 367 346 86
66 17 12
Bsu36I CC'TnA_GG
Cuts at: 2905 2905
Size: 7060
BtgI C'CryG_G
Cuts at: 513 1065 2025 3706 4254 513
Size: 552 960 1681 548 3319
Fragments arranged by size:
3319 1681 960 552 548
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6321
Fragments arranged by size:
6321 683 56
BtsI GCAGTG_nn'
Cuts at: 2410 4212 4213 4576 5370 5390 2410
Size: 1802 1 363 794 20 4080
Fragments arranged by size:
4080 1802 794 363 20 1
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7060
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7025
DraI TTT'AAA
Cuts at: 4364 4364
Size: 7060
DraIII CAC_nnn'GTG
Cuts at: 2312 2312
Size: 7060
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6067 809
Size: 507 4751 1802
Fragments arranged by size:
4751 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4507 5396 7025
Size: 54 1425 50 275 18 2677 889 1629
Cuts at: 7025 8
Size: 43
Fragments arranged by size:
2677 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4507 1537
Size: 275 2695 4090
Fragments arranged by size:
4090 2695 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2341 4990 1353
Size: 988 2649 3423
Fragments arranged by size:
3423 2649 988
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5647 6021 6167 387
Size: 1064 138 4058 374 146 1280
Fragments arranged by size:
4058 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3692 3692
Size: 7060
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2172 2520 2684 3153 3224 3293 3302
Size: 393 540 348 164 469 71 69 9
Cuts at: 3302 3308 3575 3606 4209 5123 5720 6507 6775
Size: 6 267 31 603 914 597 787 268
Cuts at: 6775 1239
Size: 1524
Fragments arranged by size:
1524 914 787 603 597 540 469 393
348 268 267 164 71 69 31 9
6
EcoO109I rG'GnC_Cy
Cuts at: 2251 2583 2874 3235 3244 3374 3798 2251
Size: 332 291 361 9 130 424 5513
Fragments arranged by size:
5513 424 361 332 291 130 9
EcoRI G'AATT_C
Cuts at: 4369 4747 4369
Size: 378 6682
FspI TGC'GCA
Cuts at: 5566 5566
Size: 7060
HaeII r_GCGC'y
Cuts at: 1678 4087 6207 1678
Size: 2409 2120 2531
Fragments arranged by size:
2531 2409 2120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2658 2690 3040 3072 3222
Size: 32 135 32 1086 32 350 32 150
Cuts at: 3222 3254 4091 4123 4316 4348 5701 5733 5775
Size: 32 837 32 193 32 1353 32 42
Cuts at: 5775 5807 1373
Size: 32 2626
Fragments arranged by size:
2626 1353 1086 837 350 193 150 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2812 4404 4650 669
Size: 988 281 874 1592 246 3079
Fragments arranged by size:
3079 1592 988 874 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7060
HpaI GTT'AAC
Cuts at: 4650 4650
Size: 7060
KpnI G_GTAC'C
Cuts at: 4385 4385
Size: 7060
MfeI C'AATT_G
Cuts at: 4659 4659
Size: 7060
MluI A'CGCG_T
Cuts at: 6936 6936
Size: 7060
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3773 6174 6358 1305
Size: 2468 2401 184 2007
Fragments arranged by size:
2468 2401 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5238
Fragments arranged by size:
5238 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3973 4047 4244 4316 5018 5377 5536
Size: 648 2807 74 197 72 702 359 159
Cuts at: 5536 518
Size: 2042
Fragments arranged by size:
2807 2042 702 648 359 197 159 74
72
MspA1I CmG'CkG
Cuts at: 1606 2042 2563 3409 3527 4476 5153 6301 6546
Size: 436 521 846 118 949 677 1148 245
Cuts at: 6546 1606
Size: 2120
Fragments arranged by size:
2120 1148 949 846 677 521 436 245
118
NcoI C'CATG_G
Cuts at: 513 1065 2025 3706 4254 513
Size: 552 960 1681 548 3319
Fragments arranged by size:
3319 1681 960 552 548
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7060
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7060
NotI GC'GGCC_GC
Cuts at: 4507 4507
Size: 7060
NspI r_CATG'y
Cuts at: 1132 1229 3816 4418 5963 1132
Size: 97 2587 602 1545 2229
Fragments arranged by size:
2587 2229 1545 602 97
PasI CC'CwG_GG
Cuts at: 1348 2248 1348
Size: 900 6160
PciI A'CATG_T
Cuts at: 5959 5959
Size: 7060
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2322 2725 3341 1108
Size: 1214 403 616 4827
Fragments arranged by size:
4827 1214 616 403
PmeI GTTT'AAAC
Cuts at: 4364 4364
Size: 7060
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5129 5161 6642 6674 5129
Size: 32 1481 32 5515
Fragments arranged by size:
5515 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2874 3798 2874
Size: 924 6136
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7060
PsiI TTA'TAA
Cuts at: 4630 4630
Size: 7060
PspOMI G'GGCC_C
Cuts at: 2251 2583 3374 2251
Size: 332 791 5937
Fragments arranged by size:
5937 791 332
PstI C_TGCA'G
Cuts at: 830 2568 2827 3414 4332 4412 830
Size: 1738 259 587 918 80 3478
Fragments arranged by size:
3478 1738 918 587 259 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7060
PvuII CAG'CTG
Cuts at: 2042 2563 3409 3527 2042
Size: 521 846 118 5575
Fragments arranged by size:
5575 846 521 118
SacI G_AGCT'C
Cuts at: 3694 3694
Size: 7060
SalI G'TCGA_C
Cuts at: 1936 4402 1936
Size: 2466 4594
SanDI GG'GwC_CC
Cuts at: 2874 2874
Size: 7060
SbfI CC_TGCA'GG
Cuts at: 4412 4412
Size: 7060
ScaI AGT'ACT
Cuts at: 5308 7020 5308
Size: 1712 5348
SexAI A'CCwGG_T
Cuts at: 2626 3980 2626
Size: 1354 5706
SfcI C'TryA_G
Cuts at: 826 1045 2564 2823 3089 3404 3410 3663 4022
Size: 219 1519 259 266 315 6 253 359
Cuts at: 4022 4328 4408 5543 6224 6415 826
Size: 306 80 1135 681 191 1471
Fragments arranged by size:
1519 1471 1135 681 359 315 306 266
259 253 219 191 80 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 3242 3242
Size: 7060
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7060
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7060
SmaI CCC'GGG
Cuts at: 1872 4387 1872
Size: 2515 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3057 3285 5164 6065 6327 820
Size: 197 926 1114 228 1879 901 262 1553
Fragments arranged by size:
1879 1553 1114 926 901 262 228 197
SnaBI TAC'GTA
Cuts at: 493 4851 493
Size: 4358 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7060
SphI G_CATG'C
Cuts at: 1132 3816 4418 1132
Size: 2684 602 3774
Fragments arranged by size:
3774 2684 602
SspI AAT'ATT
Cuts at: 5 52 2515 4984 5
Size: 47 2463 2469 2081
Fragments arranged by size:
2469 2463 2081 47
StuI AGG'CCT
Cuts at: 1517 2724 3588 3651 1517
Size: 1207 864 63 4926
Fragments arranged by size:
4926 1207 864 63
StyI C'CwwG_G
Cuts at: 513 1065 2025 2719 3477 3706 3895 4254 4499
Size: 552 960 694 758 229 189 359 245
Cuts at: 4499 513
Size: 3074
Fragments arranged by size:
3074 960 758 694 552 359 245 229
189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2239 3529 3654 5087 5104 5257 5442
Size: 1255 659 1290 125 1433 17 153 185
Cuts at: 5442 5887 325
Size: 445 1498
Fragments arranged by size:
1498 1433 1290 1255 659 445 185 153
125 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2737 5306 7018
Size: 274 80 33 51 1443 760 2569 1712
Cuts at: 7018 96
Size: 138
Fragments arranged by size:
2569 1712 1443 760 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3618 3650 4204 4236 7020 7052 3618
Size: 32 554 32 2784 32 3626
Fragments arranged by size:
3626 2784 554 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7060
XbaI T'CTAG_A
Cuts at: 4396 4396
Size: 7060
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2549 2773 3007 3080 3713 2549
Size: 224 234 73 633 5896
Fragments arranged by size:
5896 633 234 224 73
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7060
XmaI C'CCGG_G
Cuts at: 1870 4385 1870
Size: 2515 4545
XmnI GAAnn'nnTTC
Cuts at: 4001 5189 4001
Size: 1188 5872
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4868 276
Size: 53 83 186 936 3334 2468
Fragments arranged by size:
3334 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SacII SapI SfoI SrfI SwaI