Restriction map for
pFN21AB6285
(Circular) (Six-base) MAPSORT of: pFN21AB6285.seq Check: 894 from: 1 to: 7291
pFN21AB6285 7291 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5101 278
Size: 53 83 186 936 3565 2468
Fragments arranged by size:
3565 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4634 1937
Size: 2697 4594
Acc65I G'GTAC_C
Cuts at: 4612 4612
Size: 7291
AclI AA'CG_TT
Cuts at: 1563 5418 5791 1563
Size: 3855 373 3063
Fragments arranged by size:
3855 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2690 3281 3411 3594 4607 5354 6738 7006
Size: 1058 591 130 183 1013 747 1384 268
Cuts at: 7006 1632
Size: 1917
Fragments arranged by size:
1917 1384 1058 1013 747 591 268 183
130
AflII C'TTAA_G
Cuts at: 820 1017 2972 4250 820
Size: 197 1955 1278 3861
Fragments arranged by size:
3861 1955 1278 197
AflIII A'CryG_T
Cuts at: 1203 6190 7167 1203
Size: 4987 977 1327
Fragments arranged by size:
4987 1327 977
AgeI A'CCGG_T
Cuts at: 6122 6122
Size: 7291
AhdI GACnn_n'nnGTC
Cuts at: 6020 6020
Size: 7291
AleI CACnn'nnGTG
Cuts at: 1166 2120 1166
Size: 954 6337
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7291
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4040 4072 4040
Size: 32 7259
AlwNI CAG_nnn'CTG
Cuts at: 2160 2188 2741 5034 6606 2160
Size: 28 553 2293 1572 2845
Fragments arranged by size:
2845 2293 1572 553 28
ApaI G_GGCC'C
Cuts at: 3254 3542 3254
Size: 288 7003
ApaLI G'TGCA_C
Cuts at: 5348 6504 5348
Size: 1156 6135
ApoI r'AATT_y
Cuts at: 1493 2909 3768 3993 4184 4600 4826 4978 7087
Size: 1416 859 225 191 416 226 152 2109
Cuts at: 7087 1493
Size: 1697
Fragments arranged by size:
2109 1697 1416 859 416 226 225 191
152
AseI AT'TA_AT
Cuts at: 160 1032 2510 2751 5845 160
Size: 872 1478 241 3094 1606
Fragments arranged by size:
3094 1606 1478 872 241
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7291
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 4563 4616 1870
Size: 73 119 2501 53 4545
Fragments arranged by size:
4545 2501 119 73 53
AvrII C'CTAG_G
Cuts at: 2261 2261
Size: 7291
BamHI G'GATC_C
Cuts at: 4621 4988 4621
Size: 367 6924
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 2132 4612 6067 618
Size: 325 217 627 246 99 2480 1455 1842
Fragments arranged by size:
2480 1842 1455 627 325 246 217 99
BanII G_rGCy'C
Cuts at: 1391 2172 2715 3254 3542 3982 4570 4610 1391
Size: 781 543 539 288 440 588 40 4072
Fragments arranged by size:
4072 781 588 543 539 440 288 40
BbeI G_GCGC'C
Cuts at: 2037 2136 2037
Size: 99 7192
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2934 3437 3689 928
Size: 971 1035 503 252 4530
Fragments arranged by size:
4530 1035 971 503 252
BbvCI CC'TCA_GC
Cuts at: 2397 2949 2397
Size: 552 6739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5482 5516 1903
Size: 34 50 34 3461 34 3678
Fragments arranged by size:
3678 3461 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5183 6393 5183
Size: 1210 6081
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7291
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5902 136
Size: 107 122 71 631 605 4230 1525
Fragments arranged by size:
4230 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7286 7286
Size: 7291
BlpI GC'TnA_GC
Cuts at: 4113 4708 4113
Size: 595 6696
Bme1580I G_kGCm'C
Cuts at: 1790 3254 3542 5352 6508 1790
Size: 1464 288 1810 1156 2573
Fragments arranged by size:
2573 1810 1464 1156 288
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7291
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5022 5980 442
Size: 416 53 83 399 24 3605 958 1753
Fragments arranged by size:
3605 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2328 3174 3246 3495 3711 5951 1239
Size: 1089 846 72 249 216 2240 2579
Fragments arranged by size:
2579 2240 1089 846 249 216 72
Bpu10I CC'TnA_GC
Cuts at: 2397 2949 2397
Size: 552 6739
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2425 2921 3106 5416 6281 6579 2425
Size: 496 185 2310 865 298 3137
Fragments arranged by size:
3137 2310 865 496 298 185
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2414 3352 4396 5954 882
Size: 617 915 938 1044 1558 2219
Fragments arranged by size:
2219 1558 1044 938 915 617
BsaAI yAC'GTr
Cuts at: 493 1204 5082 493
Size: 711 3878 2702
Fragments arranged by size:
3878 2702 711
BsaBI GATnn'nnATC
Cuts at: 7285 7285
Size: 7291
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2034 2133 2177 5098
Size: 53 83 186 936 501 99 44 2921
Cuts at: 5098 5480 6115 275
Size: 382 635 1451
Fragments arranged by size:
2921 1451 936 635 501 382 186 99
83 53 44
BsaWI w'CCGG_w
Cuts at: 2119 2227 5724 6122 6396 6543 6954 2119
Size: 108 3497 398 274 147 411 2456
Fragments arranged by size:
3497 2456 411 398 274 147 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2131 2161 2620 2650 3079
Size: 30 165 30 728 30 459 30 429
Cuts at: 3079 3109 4040 4070 4541 4571 1178
Size: 30 931 30 471 30 3898
Fragments arranged by size:
3898 931 728 471 459 429 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2265 2385 1186
Size: 1079 120 6092
Fragments arranged by size:
6092 1079 120
BseYI C'CCAG_C
Cuts at: 2181 3476 3531 3560 6494 6990 2181
Size: 1295 55 29 2934 496 2482
Fragments arranged by size:
2934 2482 1295 496 55 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3453 1163
Size: 573 1717 5001
Fragments arranged by size:
5001 1717 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2088 4741 5502 6530 1540
Size: 275 186 87 2653 761 1028 2301
Fragments arranged by size:
2653 2301 1028 761 275 186 87
BsiHKAI G_wGCw'C
Cuts at: 2117 3982 4570 5352 5437 6508 2117
Size: 1865 588 782 85 1071 2900
Fragments arranged by size:
2900 1865 1071 782 588 85
BsmI GAATG_Cn'
Cuts at: 4802 4895 4802
Size: 93 7198
BsmBI CGTCTCn'nnnn_
Cuts at: 4350 4350
Size: 7291
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2172 2715 3254 3542 3982 4570
Size: 399 327 55 543 539 288 440 588
Cuts at: 4570 4610 5352 5437 6508 1391
Size: 40 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 588 543 539 440 399
327 288 85 55 40
BspEI T'CCGG_A
Cuts at: 6954 6954
Size: 7291
BspHI T'CATG_A
Cuts at: 5180 6910 5180
Size: 1730 5561
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4646 844
Size: 3802 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2039 5178 1394
Size: 429 216 3139 3507
Fragments arranged by size:
3507 3139 429 216
BsrDI GCAATG_nn'
Cuts at: 65 1248 2811 3038 3063 3625 5786 5960 65
Size: 1183 1563 227 25 562 2161 174 1396
Fragments arranged by size:
2161 1563 1396 1183 562 227 174 25
BsrFI r'CCGG_y
Cuts at: 1700 1810 2012 2030 2098 5935 6122 1700
Size: 110 202 18 68 3837 187 2869
Fragments arranged by size:
3837 2869 202 187 110 68 18
BsrGI T'GTAC_A
Cuts at: 96 2483 96
Size: 2387 4904
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7291
BssSI C'ACGA_G
Cuts at: 4501 5351 6363 4501
Size: 850 1012 5429
Fragments arranged by size:
5429 1012 850
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7291
BstBI TT'CG_AA
Cuts at: 6095 6095
Size: 7291
BstEII G'GTnAC_C
Cuts at: 1184 2212 2569 1184
Size: 1028 357 5906
Fragments arranged by size:
5906 1028 357
BstYI r'GATC_y
Cuts at: 1972 4282 4621 4988 5373 5390 6842 6928 6940
Size: 2310 339 367 385 17 1452 86 12
Cuts at: 6940 7286 1972
Size: 346 1977
Fragments arranged by size:
2310 1977 1452 385 367 346 339 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 4514 4514
Size: 7291
BtgI C'CryG_G
Cuts at: 513 1065 2076 2172 2199 3342 513
Size: 552 1011 96 27 1143 4462
Fragments arranged by size:
4462 1143 1011 552 96 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6552
Fragments arranged by size:
6552 683 56
BtsI GCAGTG_nn'
Cuts at: 2532 3350 4807 5601 5621 2532
Size: 818 1457 794 20 4202
Fragments arranged by size:
4202 1457 818 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7291
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7256
DraI TTT'AAA
Cuts at: 4595 4595
Size: 7291
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6298 809
Size: 507 4982 1802
Fragments arranged by size:
4982 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 4738 5627
Size: 54 1425 50 275 18 255 2653 889
Cuts at: 5627 7256 8
Size: 1629 43
Fragments arranged by size:
2653 1629 1425 889 275 255 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2085 4738 1537
Size: 275 273 2653 4090
Fragments arranged by size:
4090 2653 275 273
EarI CTCTTCn'nnn_
Cuts at: 1353 4394 5221 1353
Size: 3041 827 3423
Fragments arranged by size:
3423 3041 827
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5878 6252 6398 387
Size: 1064 138 4289 374 146 1280
Fragments arranged by size:
4289 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3980 4568 3980
Size: 588 6703
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2328 2690 3174 3246 3281 3411 3495
Size: 393 696 362 484 72 35 130 84
Cuts at: 3495 3594 3711 4607 5354 5951 6738 7006 1239
Size: 99 117 896 747 597 787 268 1524
Fragments arranged by size:
1524 896 787 747 696 597 484 393
362 268 130 117 99 84 72 35
EcoNI CCTnn'n_nnAGG
Cuts at: 3612 4586 3612
Size: 974 6317
EcoO109I rG'GnC_Cy
Cuts at: 2205 3539 2205
Size: 1334 5957
EcoRI G'AATT_C
Cuts at: 4600 4978 4600
Size: 378 6913
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4457 4457
Size: 7291
FspI TGC'GCA
Cuts at: 5797 5797
Size: 7291
HaeII r_GCGC'y
Cuts at: 1678 2037 2061 2096 2136 2238 6438 1678
Size: 359 24 35 40 102 4200 2531
Fragments arranged by size:
4200 2531 359 102 40 35 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2131 2163 4157 4189 5932
Size: 32 135 32 559 32 1994 32 1743
Cuts at: 5932 5964 6006 6038 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1994 1743 559 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3880 4635 4881 669
Size: 988 281 1942 755 246 3079
Fragments arranged by size:
3079 1942 988 755 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7291
HpaI GTT'AAC
Cuts at: 4881 4881
Size: 7291
KasI G'GCGC_C
Cuts at: 2033 2132 2033
Size: 99 7192
KpnI G_GTAC'C
Cuts at: 4616 4616
Size: 7291
MfeI C'AATT_G
Cuts at: 4107 4205 4328 4890 4107
Size: 98 123 562 6508
Fragments arranged by size:
6508 562 123 98
MluI A'CGCG_T
Cuts at: 7167 7167
Size: 7291
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2786 6405 6589 1305
Size: 1481 3619 184 2007
Fragments arranged by size:
3619 2007 1481 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5469
Fragments arranged by size:
5469 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 5249 5608 5767 518
Size: 648 954 3129 359 159 2042
Fragments arranged by size:
3129 2042 954 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2256 2741 3281 3560 4294 4707 5384 6532
Size: 650 485 540 279 734 413 677 1148
Cuts at: 6532 6777 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 734 677 650 540 485 413
279 245
NaeI GCC'GGC
Cuts at: 2014 2032 2014
Size: 18 7273
NarI GG'CG_CC
Cuts at: 2034 2133 2034
Size: 99 7192
NcoI C'CATG_G
Cuts at: 513 1065 2076 513
Size: 552 1011 5728
Fragments arranged by size:
5728 1011 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7291
NgoMIV G'CCGG_C
Cuts at: 2012 2030 2012
Size: 18 7273
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7291
NotI GC'GGCC_GC
Cuts at: 4738 4738
Size: 7291
NspI r_CATG'y
Cuts at: 1132 1229 2826 4649 6194 1132
Size: 97 1597 1823 1545 2229
Fragments arranged by size:
2229 1823 1597 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2128 1348
Size: 780 6511
PciI A'CATG_T
Cuts at: 6190 6190
Size: 7291
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3203 1108
Size: 2095 5196
PfoI T'CCnGG_A
Cuts at: 2344 2638 2344
Size: 294 6997
PmeI GTTT'AAAC
Cuts at: 4595 4595
Size: 7291
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2486 2518 4040 4072 5360 5392 6873 6905 2486
Size: 32 1522 32 1288 32 1481 32 2872
Fragments arranged by size:
2872 1522 1481 1288 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2205 2205
Size: 7291
PshAI GACnn'nnGTC
Cuts at: 1613 3344 1613
Size: 1731 5560
PsiI TTA'TAA
Cuts at: 4861 4861
Size: 7291
PspOMI G'GGCC_C
Cuts at: 3250 3538 3250
Size: 288 7003
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2562 2594 2562
Size: 32 7259
PstI C_TGCA'G
Cuts at: 830 4643 830
Size: 3813 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7291
PvuII CAG'CTG
Cuts at: 2741 3281 3560 4294 2741
Size: 540 279 734 5738
Fragments arranged by size:
5738 734 540 279
SacI G_AGCT'C
Cuts at: 3982 4570 3982
Size: 588 6703
SalI G'TCGA_C
Cuts at: 1936 4633 1936
Size: 2697 4594
SanDI GG'GwC_CC
Cuts at: 2205 2205
Size: 7291
SbfI CC_TGCA'GG
Cuts at: 4643 4643
Size: 7291
ScaI AGT'ACT
Cuts at: 5539 7251 5539
Size: 1712 5579
SexAI A'CCwGG_T
Cuts at: 2958 4169 2958
Size: 1211 6080
SfcI C'TryA_G
Cuts at: 826 1045 3111 4639 5774 6455 6646 826
Size: 219 2066 1528 1135 681 191 1471
Fragments arranged by size:
2066 1528 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2035 2134 2035
Size: 99 7192
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7291
SgrAI Cr'CCGG_yG
Cuts at: 1700 2030 1700
Size: 330 6961
SmaI CCC'GGG
Cuts at: 1872 4618 1872
Size: 2746 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2440 2936 2972 3121 4250 4563
Size: 197 926 497 496 36 149 1129 313
Cuts at: 4563 5395 6296 6558 820
Size: 832 901 262 1553
Fragments arranged by size:
1553 1129 926 901 832 497 496 313
262 197 149 36
SnaBI TAC'GTA
Cuts at: 493 5082 493
Size: 4589 2702
SpeI A'CTAG_T
Cuts at: 152 3592 3713 152
Size: 3440 121 3730
Fragments arranged by size:
3730 3440 121
SphI G_CATG'C
Cuts at: 1132 4649 1132
Size: 3517 3774
SspI AAT'ATT
Cuts at: 5 52 5215 5
Size: 47 5163 2081
Fragments arranged by size:
5163 2081 47
StuI AGG'CCT
Cuts at: 1517 4086 1517
Size: 2569 4722
StyI C'CwwG_G
Cuts at: 513 1065 2076 2261 4391 4730 513
Size: 552 1011 185 2130 339 3074
Fragments arranged by size:
3074 2130 1011 552 339 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3181 5318 5335 5488 5673 6118 325
Size: 1255 1601 2137 17 153 185 445 1498
Fragments arranged by size:
2137 1601 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2483 2596 3074
Size: 274 80 33 51 1443 506 113 478
Cuts at: 3074 5537 7249 96
Size: 2463 1712 138
Fragments arranged by size:
2463 1712 1443 506 478 274 138 113
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2550 2582 2620 2652 7251 7283 2550
Size: 32 38 32 4599 32 2558
Fragments arranged by size:
4599 2558 38 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3059 1316
Size: 1743 5548
XbaI T'CTAG_A
Cuts at: 4090 4627 4090
Size: 537 6754
XhoI C'TCGA_G
Cuts at: 1943 4563 1943
Size: 2620 4671
XmaI C'CCGG_G
Cuts at: 1870 4616 1870
Size: 2746 4545
XmnI GAAnn'nnTTC
Cuts at: 3963 5420 3963
Size: 1457 5834
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5099 276
Size: 53 83 186 936 3565 2468
Fragments arranged by size:
3565 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BfrBI BplI BsiWI BstXI
BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI
PmlI PspXI RsrII SacII SapI SfiI SrfI SwaI
XcmI