Restriction map for
pFN21AB6281
(Circular) (Six-base) MAPSORT of: pFN21AB6281.seq Check: 7010 from: 1 to: 7099
pFN21AB6281 7099 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2620 4909 278
Size: 53 83 186 936 1084 2289 2468
Fragments arranged by size:
2468 2289 1084 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4442 1937
Size: 2505 4594
Acc65I G'GTAC_C
Cuts at: 4420 4420
Size: 7099
AclI AA'CG_TT
Cuts at: 1563 5226 5599 1563
Size: 3663 373 3063
Fragments arranged by size:
3663 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2972 3959 5162 6546 6814 1632
Size: 1340 987 1203 1384 268 1917
Fragments arranged by size:
1917 1384 1340 1203 987 268
AflII C'TTAA_G
Cuts at: 820 1017 2435 2854 820
Size: 197 1418 419 5065
Fragments arranged by size:
5065 1418 419 197
AflIII A'CryG_T
Cuts at: 1203 2016 5998 6975 1203
Size: 813 3982 977 1327
Fragments arranged by size:
3982 1327 977 813
AgeI A'CCGG_T
Cuts at: 3446 5930 3446
Size: 2484 4615
AhdI GACnn_n'nnGTC
Cuts at: 2035 5828 2035
Size: 3793 3306
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7099
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7099
AlwNI CAG_nnn'CTG
Cuts at: 4842 6414 4842
Size: 1572 5527
ApaLI G'TGCA_C
Cuts at: 4120 5156 6312 4120
Size: 1036 1156 4907
Fragments arranged by size:
4907 1156 1036
ApoI r'AATT_y
Cuts at: 1493 2563 3508 3847 4408 4634 4786 6895 1493
Size: 1070 945 339 561 226 152 2109 1697
Fragments arranged by size:
2109 1697 1070 945 561 339 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3174 3198 5653 160
Size: 872 2142 24 2455 1606
Fragments arranged by size:
2455 2142 1606 872 24
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7099
AvaI C'yCGr_G
Cuts at: 1870 1943 4424 1870
Size: 73 2481 4545
Fragments arranged by size:
4545 2481 73
BamHI G'GATC_C
Cuts at: 4429 4796 4429
Size: 367 6732
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2396 2452 2974 4420 5875
Size: 325 217 627 609 56 522 1446 1455
Cuts at: 5875 618
Size: 1842
Fragments arranged by size:
1842 1455 1446 627 609 522 325 217
56
BanII G_rGCy'C
Cuts at: 1391 3216 4418 1391
Size: 1825 1202 4072
Fragments arranged by size:
4072 1825 1202
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2085 2319 3003 3125 928
Size: 971 186 234 684 122 4902
Fragments arranged by size:
4902 971 684 234 186 122
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2045 2079 3050 3084 5290 5324 1903
Size: 34 108 34 971 34 2206 34 3678
Fragments arranged by size:
3678 2206 971 108 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2137 4202 4991 6201 2137
Size: 2065 789 1210 3035
Fragments arranged by size:
3035 2065 1210 789
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7099
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5710 136
Size: 107 122 71 631 605 4038 1525
Fragments arranged by size:
4038 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2098 7094 2098
Size: 4996 2103
BlpI GC'TnA_GC
Cuts at: 4516 4516
Size: 7099
Bme1580I G_kGCm'C
Cuts at: 1790 4124 5160 6316 1790
Size: 2334 1036 1156 2573
Fragments arranged by size:
2573 2334 1156 1036
BmgBI CAC'GTC
Cuts at: 1318 2353 1318
Size: 1035 6064
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3126 3405 4830
Size: 416 53 83 399 24 1709 279 1425
Cuts at: 4830 5788 442
Size: 958 1753
Fragments arranged by size:
1753 1709 1425 958 416 399 279 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2273 1056
Size: 1217 5882
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3759 5759 1239
Size: 2520 2000 2579
Fragments arranged by size:
2579 2520 2000
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2153 5224 6089 6387 2153
Size: 3071 865 298 2865
Fragments arranged by size:
3071 2865 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2498 3340 3777 5762 882
Size: 617 999 842 437 1985 2219
Fragments arranged by size:
2219 1985 999 842 617 437
BsaAI yAC'GTr
Cuts at: 493 1204 4890 493
Size: 711 3686 2702
Fragments arranged by size:
3686 2702 711
BsaBI GATnn'nnATC
Cuts at: 7093 7093
Size: 7099
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2617 4906 5288 5923
Size: 53 83 186 936 1084 2289 382 635
Cuts at: 5923 275
Size: 1451
Fragments arranged by size:
2289 1451 1084 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3446 5532 5930 6204 6351 6762 3446
Size: 2086 398 274 147 411 3783
Fragments arranged by size:
3783 2086 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2887 2917 3198 3228 1178
Size: 30 165 30 1484 30 281 30 5049
Fragments arranged by size:
5049 1484 281 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2655 2892 2895 3241 4130 1186
Size: 1469 237 3 346 889 4155
Fragments arranged by size:
4155 1469 889 346 237 3
BseYI C'CCAG_C
Cuts at: 6302 6798 6302
Size: 496 6603
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2197 1163
Size: 573 461 6065
Fragments arranged by size:
6065 573 461
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4549 5310 6338 1540
Size: 275 186 2548 761 1028 2301
Fragments arranged by size:
2548 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3216 4124 5160 5245 6316 3216
Size: 908 1036 85 1071 3999
Fragments arranged by size:
3999 1071 1036 908 85
BsmI GAATG_Cn'
Cuts at: 2489 4610 4703 2489
Size: 2121 93 4885
Fragments arranged by size:
4885 2121 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2343 2433 2343
Size: 90 7009
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3216 4124 4418 5160 5245 6316 1391
Size: 399 1426 908 294 742 85 1071 2174
Fragments arranged by size:
2174 1426 1071 908 742 399 294 85
BspEI T'CCGG_A
Cuts at: 6762 6762
Size: 7099
BspHI T'CATG_A
Cuts at: 4988 6718 4988
Size: 1730 5369
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3322 4212 4454 844
Size: 2478 890 242 3489
Fragments arranged by size:
3489 2478 890 242
BsrBI CCG'CTC
Cuts at: 1394 1823 4986 1394
Size: 429 3163 3507
Fragments arranged by size:
3507 3163 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5594 5768 65
Size: 1183 4346 174 1396
Fragments arranged by size:
4346 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3446 5743 5930 1700
Size: 110 1636 2297 187 2869
Fragments arranged by size:
2869 2297 1636 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7099
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7099
BssSI C'ACGA_G
Cuts at: 3107 3929 5159 6171 3107
Size: 822 1230 1012 4035
Fragments arranged by size:
4035 1230 1012 822
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7099
BstBI TT'CG_AA
Cuts at: 5903 5903
Size: 7099
BstEII G'GTnAC_C
Cuts at: 1184 3149 1184
Size: 1965 5134
BstYI r'GATC_y
Cuts at: 1972 2098 3785 3797 4429 4796 5181 5198 6650
Size: 126 1687 12 632 367 385 17 1452
Cuts at: 6650 6736 6748 7094 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1687 1452 632 385 367 346 126
86 17 12 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3522 513
Size: 552 939 1518 4090
Fragments arranged by size:
4090 1518 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6360
Fragments arranged by size:
6360 683 56
BtsI GCAGTG_nn'
Cuts at: 2185 2494 4615 5409 5429 2185
Size: 309 2121 794 20 3855
Fragments arranged by size:
3855 2121 794 309 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7099
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7064
DraI TTT'AAA
Cuts at: 4403 4403
Size: 7099
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2797 6106 809
Size: 507 1481 3309 1802
Fragments arranged by size:
3309 1802 1481 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4546 5435 7064
Size: 54 1425 50 275 18 2716 889 1629
Cuts at: 7064 8
Size: 43
Fragments arranged by size:
2716 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4546 1537
Size: 275 2734 4090
Fragments arranged by size:
4090 2734 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2244 2415 2579 2787 3796 3935 4020 5029
Size: 891 171 164 208 1009 139 85 1009
Cuts at: 5029 1353
Size: 3423
Fragments arranged by size:
3423 1009 1009 891 208 171 164 139
85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5686 6060 6206 387
Size: 1064 138 4097 374 146 1280
Fragments arranged by size:
4097 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3214 3214
Size: 7099
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2972 3759 3959 5162 5759 6546 6814
Size: 393 1340 787 200 1203 597 787 268
Cuts at: 6814 1239
Size: 1524
Fragments arranged by size:
1524 1340 1203 787 787 597 393 268
200
EcoO109I rG'GnC_Cy
Cuts at: 3714 3714
Size: 7099
EcoRI G'AATT_C
Cuts at: 2563 4408 4786 2563
Size: 1845 378 4876
Fragments arranged by size:
4876 1845 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 7099
FspI TGC'GCA
Cuts at: 4220 4394 5605 4220
Size: 174 1211 5714
Fragments arranged by size:
5714 1211 174
HaeII r_GCGC'y
Cuts at: 1678 4033 6246 1678
Size: 2355 2213 2531
Fragments arranged by size:
2531 2355 2213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 2578 2610 3236
Size: 32 135 32 449 32 525 32 626
Cuts at: 3236 3268 3641 3673 3688 3720 4289 4321 5740
Size: 32 373 32 15 32 569 32 1419
Cuts at: 5740 5772 5814 5846 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1419 626 569 525 449 373 135
42 32 32 32 32 32 32 32
32 32 32 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2597 4443 4689 669
Size: 988 281 659 1846 246 3079
Fragments arranged by size:
3079 1846 988 659 281 246
HindIII A'AGCT_T
Cuts at: 748 4387 748
Size: 3639 3460
HpaI GTT'AAC
Cuts at: 4689 4689
Size: 7099
KpnI G_GTAC'C
Cuts at: 4424 4424
Size: 7099
MfeI C'AATT_G
Cuts at: 2547 4020 4698 2547
Size: 1473 678 4948
Fragments arranged by size:
4948 1473 678
MluI A'CGCG_T
Cuts at: 6975 6975
Size: 7099
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6213 6397 1305
Size: 4908 184 2007
Fragments arranged by size:
4908 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5277
Fragments arranged by size:
5277 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5057 5416 5575 518
Size: 648 3891 359 159 2042
Fragments arranged by size:
3891 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4515 5192 6340 6585 1606
Size: 2909 677 1148 245 2120
Fragments arranged by size:
2909 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3522 513
Size: 552 939 1518 4090
Fragments arranged by size:
4090 1518 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7099
NheI G'CTAG_C
Cuts at: 1052 2269 1052
Size: 1217 5882
NotI GC'GGCC_GC
Cuts at: 4546 4546
Size: 7099
NspI r_CATG'y
Cuts at: 1132 1229 2020 4457 6002 1132
Size: 97 791 2437 1545 2229
Fragments arranged by size:
2437 2229 1545 791 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7099
PciI A'CATG_T
Cuts at: 2016 5998 2016
Size: 3982 3117
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 6197
PmeI GTTT'AAAC
Cuts at: 4403 4403
Size: 7099
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3856 3888 5168 5200 6681 6713 3856
Size: 32 1280 32 1481 32 4242
Fragments arranged by size:
4242 1481 1280 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3714 3714
Size: 7099
PshAI GACnn'nnGTC
Cuts at: 1613 3730 1613
Size: 2117 4982
PsiI TTA'TAA
Cuts at: 2478 3506 4669 2478
Size: 1028 1163 4908
Fragments arranged by size:
4908 1163 1028
PstI C_TGCA'G
Cuts at: 830 2881 4451 830
Size: 2051 1570 3478
Fragments arranged by size:
3478 2051 1570
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7099
SacI G_AGCT'C
Cuts at: 3216 3216
Size: 7099
SalI G'TCGA_C
Cuts at: 1936 4441 1936
Size: 2505 4594
SapI GCTCTTCn'nnn_
Cuts at: 2415 4020 2415
Size: 1605 5494
SbfI CC_TGCA'GG
Cuts at: 4451 4451
Size: 7099
ScaI AGT'ACT
Cuts at: 5347 7059 5347
Size: 1712 5387
SfcI C'TryA_G
Cuts at: 826 1045 2877 3826 3886 4447 5582 6263 6454
Size: 219 1832 949 60 561 1135 681 191
Cuts at: 6454 826
Size: 1471
Fragments arranged by size:
1832 1471 1135 949 681 561 219 191
60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7099
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7099
SmaI CCC'GGG
Cuts at: 1872 4426 1872
Size: 2554 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2168 2435 2854 5203 6104 6366
Size: 197 926 225 267 419 2349 901 262
Cuts at: 6366 820
Size: 1553
Fragments arranged by size:
2349 1553 926 901 419 267 262 225
197
SnaBI TAC'GTA
Cuts at: 493 4890 493
Size: 4397 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7099
SphI G_CATG'C
Cuts at: 1132 4457 1132
Size: 3325 3774
SspI AAT'ATT
Cuts at: 5 52 5023 5
Size: 47 4971 2081
Fragments arranged by size:
4971 2081 47
StuI AGG'CCT
Cuts at: 1517 2883 1517
Size: 1366 5733
StyI C'CwwG_G
Cuts at: 513 1065 2004 2814 3522 3575 4538 513
Size: 552 939 810 708 53 963 3074
Fragments arranged by size:
3074 963 939 810 708 552 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2150 2908 3392 3679 5126 5143 5296
Size: 1255 570 758 484 287 1447 17 153
Cuts at: 5296 5481 5926 325
Size: 185 445 1498
Fragments arranged by size:
1498 1447 1255 758 570 484 445 287
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2208 4248 5345
Size: 274 80 33 51 1443 231 2040 1097
Cuts at: 5345 7057 96
Size: 1712 138
Fragments arranged by size:
2040 1712 1443 1097 274 231 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2887 2919 3198 3230 7059 7091 2887
Size: 32 279 32 3829 32 2895
Fragments arranged by size:
3829 2895 279 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7099
XbaI T'CTAG_A
Cuts at: 3224 4435 3224
Size: 1211 5888
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7099
XmaI C'CCGG_G
Cuts at: 1870 4424 1870
Size: 2554 4545
XmnI GAAnn'nnTTC
Cuts at: 5228 5228
Size: 7099
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2618 4907 276
Size: 53 83 186 936 1084 2289 2468
Fragments arranged by size:
2468 2289 1084 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI