Restriction map for
pFN21AB6279
(Circular) (Six-base) MAPSORT of: pFN21AB6279.seq Check: 692 from: 1 to: 7087
pFN21AB6279 7087 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4897 278
Size: 53 83 186 936 3361 2468
Fragments arranged by size:
3361 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2111 2418 4430 1937
Size: 174 307 2012 4594
Fragments arranged by size:
4594 2012 307 174
Acc65I G'GTAC_C
Cuts at: 3479 4408 3479
Size: 929 6158
AclI AA'CG_TT
Cuts at: 1563 2449 5214 5587 1563
Size: 886 2765 373 3063
Fragments arranged by size:
3063 2765 886 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2630 3667 3714 3720 5150 6534 6802 1632
Size: 998 1037 47 6 1430 1384 268 1917
Fragments arranged by size:
1917 1430 1384 1037 998 268 47 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6890
AflIII A'CryG_T
Cuts at: 1203 2569 3804 5986 6963 1203
Size: 1366 1235 2182 977 1327
Fragments arranged by size:
2182 1366 1327 1235 977
AgeI A'CCGG_T
Cuts at: 5918 5918
Size: 7087
AhdI GACnn_n'nnGTC
Cuts at: 5816 5816
Size: 7087
AleI CACnn'nnGTG
Cuts at: 1166 2520 1166
Size: 1354 5733
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2550 3972 1184
Size: 1366 1422 4299
Fragments arranged by size:
4299 1422 1366
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2232 2264 2434 2466 4185 4217 2232
Size: 32 170 32 1719 32 5102
Fragments arranged by size:
5102 1719 170 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3217 3571 3634 3698 4830 6402 3217
Size: 354 63 64 1132 1572 3902
Fragments arranged by size:
3902 1572 1132 354 64 63
ApaI G_GGCC'C
Cuts at: 3366 3366
Size: 7087
ApaLI G'TGCA_C
Cuts at: 5144 6300 5144
Size: 1156 5931
ApoI r'AATT_y
Cuts at: 1493 3724 3994 4298 4307 4396 4622 4774 6883
Size: 2231 270 304 9 89 226 152 2109
Cuts at: 6883 1493
Size: 1697
Fragments arranged by size:
2231 2109 1697 304 270 226 152 89
9
AseI AT'TA_AT
Cuts at: 160 1032 3189 5641 160
Size: 872 2157 2452 1606
Fragments arranged by size:
2452 2157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7087
AvaI C'yCGr_G
Cuts at: 1870 1943 3880 4412 1870
Size: 73 1937 532 4545
Fragments arranged by size:
4545 1937 532 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2355 2388 2621 2654 3687 3720 2355
Size: 33 233 33 1033 33 5722
Fragments arranged by size:
5722 1033 233 33 33 33
BamHI G'GATC_C
Cuts at: 4417 4784 4417
Size: 367 6720
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3479 4408 5863 618
Size: 325 217 627 1692 929 1455 1842
Fragments arranged by size:
1842 1692 1455 929 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3131 3366 4187 4406 1391
Size: 1740 235 821 219 4072
Fragments arranged by size:
4072 1740 821 235 219
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2061 2566 928
Size: 971 162 505 5449
Fragments arranged by size:
5449 971 505 162
BbvCI CC'TCA_GC
Cuts at: 4149 4149
Size: 7087
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5278 5312 1903
Size: 34 3341 34 3678
Fragments arranged by size:
3678 3341 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4979 6189 4979
Size: 1210 5877
BclI T'GATC_A
Cuts at: 1548 3337 1548
Size: 1789 5298
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5698 136
Size: 107 122 71 631 605 4026 1525
Fragments arranged by size:
4026 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7082 7082
Size: 7087
BlpI GC'TnA_GC
Cuts at: 4504 4504
Size: 7087
Bme1580I G_kGCm'C
Cuts at: 1790 3366 5148 6304 1790
Size: 1576 1782 1156 2573
Fragments arranged by size:
2573 1782 1576 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7087
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3360 4818 5776
Size: 416 53 83 399 24 1943 1458 958
Cuts at: 5776 442
Size: 1753
Fragments arranged by size:
1943 1753 1458 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7087
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2142 3239 3999 5747 1239
Size: 903 1097 760 1748 2579
Fragments arranged by size:
2579 1748 1097 903 760
Bpu10I CC'TnA_GC
Cuts at: 4149 4149
Size: 7087
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2292 5212 6077 6375 2292
Size: 2920 865 298 3004
Fragments arranged by size:
3004 2920 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5750 882
Size: 617 4251 2219
Fragments arranged by size:
4251 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4878 493
Size: 711 3674 2702
Fragments arranged by size:
3674 2702 711
BsaBI GATnn'nnATC
Cuts at: 7081 7081
Size: 7087
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4894 5276 5911 275
Size: 53 83 186 936 3361 382 635 1451
Fragments arranged by size:
3361 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5520 5918 6192 6339 6750 5520
Size: 398 274 147 411 5857
Fragments arranged by size:
5857 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6862
Fragments arranged by size:
6862 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 1186
Size: 1084 6003
BseYI C'CCAG_C
Cuts at: 4155 4340 6290 6786 4155
Size: 185 1950 496 4456
Fragments arranged by size:
4456 1950 496 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6514
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4537 5298 6326 1540
Size: 275 186 2536 761 1028 2301
Fragments arranged by size:
2536 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2529 4187 4210 5148 5233 6304 2529
Size: 1658 23 938 85 1071 3312
Fragments arranged by size:
3312 1658 1071 938 85 23
BsmI GAATG_Cn'
Cuts at: 3929 4598 4691 3929
Size: 669 93 6325
Fragments arranged by size:
6325 669 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2529 3131 3366 4187 4210 4406 5148
Size: 399 739 602 235 821 23 196 742
Cuts at: 5148 5233 6304 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 821 742 739 602 399 235
196 85 23
BspEI T'CCGG_A
Cuts at: 6750 6750
Size: 7087
BspHI T'CATG_A
Cuts at: 2845 3595 4976 6706 2845
Size: 750 1381 1730 3226
Fragments arranged by size:
3226 1730 1381 750
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2649 3940 4442 844
Size: 1805 1291 502 3489
Fragments arranged by size:
3489 1805 1291 502
BsrBI CCG'CTC
Cuts at: 1394 1823 4974 1394
Size: 429 3151 3507
Fragments arranged by size:
3507 3151 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2539 3331 5582 5756 65
Size: 1183 1291 792 2251 174 1396
Fragments arranged by size:
2251 1396 1291 1183 792 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5731 5918 1700
Size: 110 3921 187 2869
Fragments arranged by size:
3921 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7087
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7087
BssSI C'ACGA_G
Cuts at: 5147 6159 5147
Size: 1012 6075
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3095 794
Size: 2301 4786
BstBI TT'CG_AA
Cuts at: 5891 5891
Size: 7087
BstEII G'GTnAC_C
Cuts at: 1184 3745 1184
Size: 2561 4526
BstXI CCAn_nnnn'nTGG
Cuts at: 2080 4382 2080
Size: 2302 4785
BstYI r'GATC_y
Cuts at: 1972 2172 4417 4784 5169 5186 6638 6724 6736
Size: 200 2245 367 385 17 1452 86 12
Cuts at: 6736 7082 1972
Size: 346 1977
Fragments arranged by size:
2245 1977 1452 385 367 346 200 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3543 513
Size: 552 2478 4057
Fragments arranged by size:
4057 2478 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6348
Fragments arranged by size:
6348 683 56
BtsI GCAGTG_nn'
Cuts at: 2527 2936 4251 4603 5397 5417 2527
Size: 409 1315 352 794 20 4197
Fragments arranged by size:
4197 1315 794 409 352 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7087
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7052
DraI TTT'AAA
Cuts at: 4391 4391
Size: 7087
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3950 6094 809
Size: 507 2634 2144 1802
Fragments arranged by size:
2634 2144 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2104 4372 4534
Size: 54 1425 50 275 18 274 2268 162
Cuts at: 4534 5423 7052 8
Size: 889 1629 43
Fragments arranged by size:
2268 1629 1425 889 275 274 162 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4534 1537
Size: 275 2722 4090
Fragments arranged by size:
4090 2722 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2271 3252 3940 5017 1353
Size: 918 981 688 1077 3423
Fragments arranged by size:
3423 1077 981 918 688
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5674 6048 6194 387
Size: 1064 138 4085 374 146 1280
Fragments arranged by size:
4085 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4185 4185
Size: 7087
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2630 3239 3667 3714 3720 3999
Size: 393 510 488 609 428 47 6 279
Cuts at: 3999 5150 5747 6534 6802 1239
Size: 1151 597 787 268 1524
Fragments arranged by size:
1524 1151 787 609 597 510 488 428
393 279 268 47 6
EcoO109I rG'GnC_Cy
Cuts at: 3362 3362
Size: 7087
EcoRI G'AATT_C
Cuts at: 3994 4396 4774 3994
Size: 402 378 6307
Fragments arranged by size:
6307 402 378
EcoRV GAT'ATC
Cuts at: 2229 3678 2229
Size: 1449 5638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2900 4045 2900
Size: 1145 5942
FspI TGC'GCA
Cuts at: 5593 5593
Size: 7087
HaeII r_GCGC'y
Cuts at: 1678 6234 1678
Size: 4556 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2213 2245 2471 2503 2856
Size: 32 135 32 641 32 226 32 353
Cuts at: 2856 2888 2957 2989 5728 5760 5802 5834 1373
Size: 32 69 32 2739 32 42 32 2626
Fragments arranged by size:
2739 2626 641 353 226 135 69 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4431 4677 669
Size: 988 281 2493 246 3079
Fragments arranged by size:
3079 2493 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4170 748
Size: 3422 3665
HpaI GTT'AAC
Cuts at: 4677 4677
Size: 7087
KpnI G_GTAC'C
Cuts at: 3483 4412 3483
Size: 929 6158
MfeI C'AATT_G
Cuts at: 4686 4686
Size: 7087
MluI A'CGCG_T
Cuts at: 6963 6963
Size: 7087
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3618 3745 6201 6385 1305
Size: 2313 127 2456 184 2007
Fragments arranged by size:
2456 2313 2007 184 127
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2106 4374 10
Size: 54 1425 343 274 2268 2723
Fragments arranged by size:
2723 2268 1425 343 274 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2520 2532 2586 2602 2670 5045 5404
Size: 648 1354 12 54 16 68 2375 359
Cuts at: 5404 5563 518
Size: 159 2042
Fragments arranged by size:
2375 2042 1354 648 359 159 68 54
16 12
MspA1I CmG'CkG
Cuts at: 1606 3515 4503 5180 6328 6573 1606
Size: 1909 988 677 1148 245 2120
Fragments arranged by size:
2120 1909 1148 988 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6535
NdeI CA'TA_TG
Cuts at: 387 2727 3253 387
Size: 2340 526 4221
Fragments arranged by size:
4221 2340 526
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7087
NotI GC'GGCC_GC
Cuts at: 4534 4534
Size: 7087
NspI r_CATG'y
Cuts at: 1132 1229 2573 2623 3808 4445 5990 1132
Size: 97 1344 50 1185 637 1545 2229
Fragments arranged by size:
2229 1545 1344 1185 637 97 50
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7087
PciI A'CATG_T
Cuts at: 2569 3804 5986 2569
Size: 1235 2182 3670
Fragments arranged by size:
3670 2182 1235
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7087
PfoI T'CCnGG_A
Cuts at: 2072 2072
Size: 7087
PmeI GTTT'AAAC
Cuts at: 4391 4391
Size: 7087
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5156 5188 6669 6701 5156
Size: 32 1481 32 5542
Fragments arranged by size:
5542 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7087
PsiI TTA'TAA
Cuts at: 4657 4657
Size: 7087
PspOMI G'GGCC_C
Cuts at: 3362 3362
Size: 7087
PstI C_TGCA'G
Cuts at: 830 2185 3630 3834 4439 830
Size: 1355 1445 204 605 3478
Fragments arranged by size:
3478 1445 1355 605 204
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7087
PvuII CAG'CTG
Cuts at: 3515 3515
Size: 7087
SacI G_AGCT'C
Cuts at: 4187 4187
Size: 7087
SalI G'TCGA_C
Cuts at: 1936 4429 1936
Size: 2493 4594
SapI GCTCTTCn'nnn_
Cuts at: 2271 2271
Size: 7087
SbfI CC_TGCA'GG
Cuts at: 4439 4439
Size: 7087
ScaI AGT'ACT
Cuts at: 2142 3061 3388 5335 7047 2142
Size: 919 327 1947 1712 2182
Fragments arranged by size:
2182 1947 1712 919 327
SfcI C'TryA_G
Cuts at: 826 1045 2129 2181 2315 3626 3830 4435 5570
Size: 219 1084 52 134 1311 204 605 1135
Cuts at: 5570 6251 6442 826
Size: 681 191 1471
Fragments arranged by size:
1471 1311 1135 1084 681 605 219 204
191 134 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7087
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7087
SmaI CCC'GGG
Cuts at: 1872 4414 1872
Size: 2542 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2307 5191 6092 6354 820
Size: 197 926 364 2884 901 262 1553
Fragments arranged by size:
2884 1553 926 901 364 262 197
SnaBI TAC'GTA
Cuts at: 493 4878 493
Size: 4385 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7087
SphI G_CATG'C
Cuts at: 1132 4445 1132
Size: 3313 3774
SspI AAT'ATT
Cuts at: 5 52 5011 5
Size: 47 4959 2081
Fragments arranged by size:
4959 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7087
StyI C'CwwG_G
Cuts at: 513 1065 4314 4526 513
Size: 552 3249 212 3074
Fragments arranged by size:
3249 3074 552 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2081 3177 5114 5131 5284 5469 5914
Size: 1255 501 1096 1937 17 153 185 445
Cuts at: 5914 325
Size: 1498
Fragments arranged by size:
1937 1498 1255 1096 501 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2140 3059 3386
Size: 274 80 33 51 1443 163 919 327
Cuts at: 3386 5333 7045 96
Size: 1947 1712 138
Fragments arranged by size:
1947 1712 1443 919 327 274 163 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2316 2348 7047 7079 2316
Size: 32 4699 32 2324
Fragments arranged by size:
4699 2324 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7087
XbaI T'CTAG_A
Cuts at: 4423 4423
Size: 7087
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3659 3659
Size: 7087
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7087
XmaI C'CCGG_G
Cuts at: 1870 4412 1870
Size: 2542 4545
XmnI GAAnn'nnTTC
Cuts at: 2301 3994 5216 2301
Size: 1693 1222 4172
Fragments arranged by size:
4172 1693 1222
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4895 276
Size: 53 83 186 936 3361 2468
Fragments arranged by size:
3361 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI