Restriction map for
pFN21AB6274
(Circular) (Six-base) MAPSORT of: pFN21AB6274.seq Check: 4239 from: 1 to: 6796
pFN21AB6274 6796 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4606 278
Size: 53 83 186 936 3070 2468
Fragments arranged by size:
3070 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4139 1937
Size: 2202 4594
Acc65I G'GTAC_C
Cuts at: 4117 4117
Size: 6796
AclI AA'CG_TT
Cuts at: 1563 4923 5296 1563
Size: 3360 373 3063
Fragments arranged by size:
3360 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2618 2717 3040 3467 4859 6243 6511 1632
Size: 986 99 323 427 1392 1384 268 1917
Fragments arranged by size:
1917 1392 1384 986 427 323 268 99
AfeI AGC'GCT
Cuts at: 3011 3963 3011
Size: 952 5844
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6599
AflIII A'CryG_T
Cuts at: 1203 3205 5695 6672 1203
Size: 2002 2490 977 1327
Fragments arranged by size:
2490 2002 1327 977
AgeI A'CCGG_T
Cuts at: 5627 5627
Size: 6796
AhdI GACnn_n'nnGTC
Cuts at: 5525 5525
Size: 6796
AleI CACnn'nnGTG
Cuts at: 1166 3194 4028 1166
Size: 2028 834 3934
Fragments arranged by size:
3934 2028 834
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4071 1184
Size: 2887 3909
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3810 3842 3810
Size: 32 6764
AlwNI CAG_nnn'CTG
Cuts at: 2157 2194 2267 3809 4082 4539 6111 2157
Size: 37 73 1542 273 457 1572 2842
Fragments arranged by size:
2842 1572 1542 457 273 73 37
ApaI G_GGCC'C
Cuts at: 2021 2279 2960 2021
Size: 258 681 5857
Fragments arranged by size:
5857 681 258
ApaLI G'TGCA_C
Cuts at: 2378 2417 3831 4046 4853 6009 2378
Size: 39 1414 215 807 1156 3165
Fragments arranged by size:
3165 1414 1156 807 215 39
ApoI r'AATT_y
Cuts at: 1493 2240 4105 4331 4483 6592 1493
Size: 747 1865 226 152 2109 1697
Fragments arranged by size:
2109 1865 1697 747 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5350 160
Size: 872 4318 1606
Fragments arranged by size:
4318 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6796
AvaI C'yCGr_G
Cuts at: 1870 1943 3527 3741 4121 1870
Size: 73 1584 214 380 4545
Fragments arranged by size:
4545 1584 380 214 73
BamHI G'GATC_C
Cuts at: 4126 4493 4126
Size: 367 6429
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2481 2809 3955 4117 5572
Size: 325 217 627 694 328 1146 162 1455
Cuts at: 5572 618
Size: 1842
Fragments arranged by size:
1842 1455 1146 694 627 328 325 217
162
BanII G_rGCy'C
Cuts at: 1391 2021 2077 2279 2506 2960 3122 3132 3369
Size: 630 56 202 227 454 162 10 237
Cuts at: 3369 3803 4115 1391
Size: 434 312 4072
Fragments arranged by size:
4072 630 454 434 312 237 227 202
162 56 10
BbeI G_GCGC'C
Cuts at: 2485 2813 3959 2485
Size: 328 1146 5322
Fragments arranged by size:
5322 1146 328
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2642 3514 928
Size: 971 743 872 4210
Fragments arranged by size:
4210 971 872 743
BbvCI CC'TCA_GC
Cuts at: 3474 3474
Size: 6796
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4987 5021 1903
Size: 34 3050 34 3678
Fragments arranged by size:
3678 3050 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4688 5898 4688
Size: 1210 5586
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6796
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3912 3921 5407
Size: 107 122 71 631 605 2240 9 1486
Cuts at: 5407 136
Size: 1525
Fragments arranged by size:
2240 1525 1486 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 3144 6791 3144
Size: 3647 3149
BlpI GC'TnA_GC
Cuts at: 2723 4213 2723
Size: 1490 5306
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2279 2382 2421 2445 2960 3835 4050
Size: 231 258 103 39 24 515 875 215
Cuts at: 4050 4089 4857 6013 1790
Size: 39 768 1156 2573
Fragments arranged by size:
2573 1156 875 768 515 258 231 215
103 39 39 24
BmgBI CAC'GTC
Cuts at: 1318 3160 1318
Size: 1842 4954
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2752 3868 4070
Size: 416 53 83 399 24 1335 1116 202
Cuts at: 4070 4527 5485 442
Size: 457 958 1753
Fragments arranged by size:
1753 1335 1116 958 457 416 399 202
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2513 2885 3957 5456 1239
Size: 1274 372 1072 1499 2579
Fragments arranged by size:
2579 1499 1274 1072 372
Bpu10I CC'TnA_GC
Cuts at: 3474 3802 3474
Size: 328 6468
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2789 4921 5786 6084 2789
Size: 2132 865 298 3501
Fragments arranged by size:
3501 2132 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2579 2672 5459 882
Size: 617 1080 93 2787 2219
Fragments arranged by size:
2787 2219 1080 617 93
BsaAI yAC'GTr
Cuts at: 493 1204 2527 4030 4587 493
Size: 711 1323 1503 557 2702
Fragments arranged by size:
2702 1503 1323 711 557
BsaBI GATnn'nnATC
Cuts at: 6790 6790
Size: 6796
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2482 2790 2810 3037
Size: 53 83 186 936 949 308 20 227
Cuts at: 3037 3956 4603 4985 5620 275
Size: 919 647 382 635 1451
Fragments arranged by size:
1451 949 936 919 647 635 382 308
227 186 83 53 20
BsaWI w'CCGG_w
Cuts at: 2748 5229 5627 5901 6048 6459 2748
Size: 2481 398 274 147 411 3085
Fragments arranged by size:
3085 2481 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2342 2372 2738 2768 2840
Size: 30 165 30 939 30 366 30 72
Cuts at: 2840 2870 3677 3707 1178
Size: 30 807 30 4267
Fragments arranged by size:
4267 939 807 366 165 72 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2354 2861 2888 3698 3701 1186
Size: 1168 507 27 810 3 4281
Fragments arranged by size:
4281 1168 810 507 27 3
BseYI C'CCAG_C
Cuts at: 2267 3217 3258 3349 5999 6495 2267
Size: 950 41 91 2650 496 2568
Fragments arranged by size:
2650 2568 950 496 91 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2143 3026 3172 1163
Size: 573 407 883 146 4787
Fragments arranged by size:
4787 883 573 407 146
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3241 4246 5007 6035 1540
Size: 275 186 1240 1005 761 1028 2301
Fragments arranged by size:
2301 1240 1028 1005 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2382 2421 3132 3835 4050 4857 4942 6013 2382
Size: 39 711 703 215 807 85 1071 3165
Fragments arranged by size:
3165 1071 807 711 703 215 85 39
BsmI GAATG_Cn'
Cuts at: 4307 4400 4307
Size: 93 6703
BsmBI CGTCTCn'nnnn_
Cuts at: 2797 2797
Size: 6796
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2077 2279 2382 2421 2445 2506
Size: 399 231 56 202 103 39 24 61
Cuts at: 2506 2960 3122 3132 3369 3803 3835 4050 4089
Size: 454 162 10 237 434 32 215 39
Cuts at: 4089 4115 4857 4942 6013 1391
Size: 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 454 434 399 237 231
215 202 162 103 85 61 56 39
39 32 26 24 10
BspEI T'CCGG_A
Cuts at: 6459 6459
Size: 6796
BspHI T'CATG_A
Cuts at: 4685 6415 4685
Size: 1730 5066
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4151 844
Size: 3307 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2123 2944 3865 4683 1394
Size: 429 300 821 921 818 3507
Fragments arranged by size:
3507 921 821 818 429 300
BsrDI GCAATG_nn'
Cuts at: 65 1248 2204 3582 5291 5465 65
Size: 1183 956 1378 1709 174 1396
Fragments arranged by size:
1709 1396 1378 1183 956 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2421 2718 2869 3240 5440 5627 1700
Size: 110 611 297 151 371 2200 187 2869
Fragments arranged by size:
2869 2200 611 371 297 187 151 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6796
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6796
BssSI C'ACGA_G
Cuts at: 4856 5868 4856
Size: 1012 5784
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2157 2194 2394 3549 794
Size: 1363 37 200 1155 4041
Fragments arranged by size:
4041 1363 1155 200 37
BstBI TT'CG_AA
Cuts at: 5600 5600
Size: 6796
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6796
BstXI CCAn_nnnn'nTGG
Cuts at: 3599 3599
Size: 6796
BstYI r'GATC_y
Cuts at: 1972 2496 2520 3144 3882 4126 4493 4878 4895
Size: 524 24 624 738 244 367 385 17
Cuts at: 4895 6347 6433 6445 6791 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 738 624 524 385 367 346
244 86 24 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2476 2814 3750 513
Size: 552 939 472 338 936 3559
Fragments arranged by size:
3559 939 936 552 472 338
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6057
Fragments arranged by size:
6057 683 56
BtsI GCAGTG_nn'
Cuts at: 2155 4312 5106 5126 2155
Size: 2157 794 20 3825
Fragments arranged by size:
3825 2157 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6796
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6761
DraI TTT'AAA
Cuts at: 4100 4100
Size: 6796
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3164 3983 5803 809
Size: 507 1848 819 1820 1802
Fragments arranged by size:
1848 1820 1802 819 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2874 3105 3238
Size: 54 1425 50 275 18 1044 231 133
Cuts at: 3238 3753 3913 3990 4243 5132 6761 8
Size: 515 160 77 253 889 1629 43
Fragments arranged by size:
1629 1425 1044 889 515 275 253 231
160 133 77 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3238 4243 1537
Size: 275 1426 1005 4090
Fragments arranged by size:
4090 1426 1005 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2968 3413 4726 1353
Size: 1615 445 1313 3423
Fragments arranged by size:
3423 1615 1313 445
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2303 5383 5757 5903 387
Size: 1064 138 714 3080 374 146 1280
Fragments arranged by size:
3080 1280 1064 714 374 146 138
EcoICRI GAG'CTC
Cuts at: 3130 3130
Size: 6796
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2513 2618 2717 2885 3040 3467 3957
Size: 393 881 105 99 168 155 427 490
Cuts at: 3957 4859 5456 6243 6511 1239
Size: 902 597 787 268 1524
Fragments arranged by size:
1524 902 881 787 597 490 427 393
268 168 155 105 99
EcoO109I rG'GnC_Cy
Cuts at: 2018 2045 2191 2275 2276 2957 3230 3442 3555
Size: 27 146 84 1 681 273 212 113
Cuts at: 3555 3806 2018
Size: 251 5008
Fragments arranged by size:
5008 681 273 251 212 146 113 84
27 1
EcoRI G'AATT_C
Cuts at: 4105 4483 4105
Size: 378 6418
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 6796
FseI GG_CCGG'CC
Cuts at: 3244 3244
Size: 6796
FspI TGC'GCA
Cuts at: 5302 5302
Size: 6796
HaeII r_GCGC'y
Cuts at: 1678 2268 2428 2476 2485 2719 2813 3013 3218
Size: 590 160 48 9 234 94 200 205
Cuts at: 3218 3796 3959 3965 5943 1678
Size: 578 163 6 1978 2531
Fragments arranged by size:
2531 1978 590 578 234 205 200 163
160 94 48 9 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2488 2520 2738 2770 3605
Size: 32 135 32 916 32 218 32 835
Cuts at: 3605 3637 3867 3874 3899 3906 5437 5469 5511
Size: 32 230 7 25 7 1531 32 42
Cuts at: 5511 5543 1373
Size: 32 2626
Fragments arranged by size:
2626 1531 916 835 230 218 135 42
32 32 32 32 32 32 32 25
7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 4140 4386 669
Size: 988 281 2202 246 3079
Fragments arranged by size:
3079 2202 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6796
HpaI GTT'AAC
Cuts at: 4386 4386
Size: 6796
KasI G'GCGC_C
Cuts at: 2481 2809 3955 2481
Size: 328 1146 5322
Fragments arranged by size:
5322 1146 328
KpnI G_GTAC'C
Cuts at: 4121 4121
Size: 6796
MfeI C'AATT_G
Cuts at: 4395 4395
Size: 6796
MluI A'CGCG_T
Cuts at: 6672 6672
Size: 6796
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3075 5910 6094 1305
Size: 1770 2835 184 2007
Fragments arranged by size:
2835 2007 1770 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3755 3992 10
Size: 54 1425 343 1923 237 2814
Fragments arranged by size:
2814 1923 1425 343 237 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3194 4028 4053 4754 5113 5272 518
Size: 648 2028 834 25 701 359 159 2042
Fragments arranged by size:
2042 2028 834 701 648 359 159 25
MspA1I CmG'CkG
Cuts at: 1606 2478 2491 3349 3849 3921 4212 4889 6037
Size: 872 13 858 500 72 291 677 1148
Cuts at: 6037 6282 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 872 858 677 500 291 245
72 13
NaeI GCC'GGC
Cuts at: 2720 3242 2720
Size: 522 6274
NarI GG'CG_CC
Cuts at: 2482 2810 3956 2482
Size: 328 1146 5322
Fragments arranged by size:
5322 1146 328
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5305
Fragments arranged by size:
5305 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6796
NgoMIV G'CCGG_C
Cuts at: 2718 3240 2718
Size: 522 6274
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6796
NotI GC'GGCC_GC
Cuts at: 4243 4243
Size: 6796
NspI r_CATG'y
Cuts at: 1132 1229 3508 4154 5699 1132
Size: 97 2279 646 1545 2229
Fragments arranged by size:
2279 2229 1545 646 97
PasI CC'CwG_GG
Cuts at: 1348 3785 1348
Size: 2437 4359
PciI A'CATG_T
Cuts at: 5695 5695
Size: 6796
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2267 1108
Size: 1159 5637
PfoI T'CCnGG_A
Cuts at: 3031 3168 3031
Size: 137 6659
PmeI GTTT'AAAC
Cuts at: 4100 4100
Size: 6796
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4865 4897 6378 6410 4865
Size: 32 1481 32 5251
Fragments arranged by size:
5251 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2045 3230 3442 3555 2045
Size: 1185 212 113 5286
Fragments arranged by size:
5286 1185 212 113
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6796
PsiI TTA'TAA
Cuts at: 4366 4366
Size: 6796
PspOMI G'GGCC_C
Cuts at: 2017 2275 2956 2017
Size: 258 681 5857
Fragments arranged by size:
5857 681 258
PstI C_TGCA'G
Cuts at: 830 2153 2213 2490 2894 4148 830
Size: 1323 60 277 404 1254 3478
Fragments arranged by size:
3478 1323 1254 404 277 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6796
PvuII CAG'CTG
Cuts at: 2491 3349 2491
Size: 858 5938
RsrII CG'GwC_CG
Cuts at: 3286 3286
Size: 6796
SacI G_AGCT'C
Cuts at: 3132 3132
Size: 6796
SacII CC_GC'GG
Cuts at: 2479 2479
Size: 6796
SalI G'TCGA_C
Cuts at: 1936 4138 1936
Size: 2202 4594
SanDI GG'GwC_CC
Cuts at: 2045 3555 2045
Size: 1510 5286
SbfI CC_TGCA'GG
Cuts at: 4148 4148
Size: 6796
ScaI AGT'ACT
Cuts at: 5044 6756 5044
Size: 1712 5084
SfcI C'TryA_G
Cuts at: 826 1045 2149 2209 2326 2486 2890 3436 4144
Size: 219 1104 60 117 160 404 546 708
Cuts at: 4144 5279 5960 6151 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1104 708 681 546 404 219
191 160 117 60
SfoI GGC'GCC
Cuts at: 2483 2811 3957 2483
Size: 328 1146 5322
Fragments arranged by size:
5322 1146 328
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6796
SgrAI Cr'CCGG_yG
Cuts at: 1700 2421 1700
Size: 721 6075
SmaI CCC'GGG
Cuts at: 1872 3529 3743 4123 1872
Size: 1657 214 380 4545
Fragments arranged by size:
4545 1657 380 214
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2804 4900 5801 6063 820
Size: 197 926 861 2096 901 262 1553
Fragments arranged by size:
2096 1553 926 901 861 262 197
SnaBI TAC'GTA
Cuts at: 493 4587 493
Size: 4094 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6796
SphI G_CATG'C
Cuts at: 1132 4154 1132
Size: 3022 3774
SspI AAT'ATT
Cuts at: 5 52 4720 5
Size: 47 4668 2081
Fragments arranged by size:
4668 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6796
StyI C'CwwG_G
Cuts at: 513 1065 2004 4235 513
Size: 552 939 2231 3074
Fragments arranged by size:
3074 2231 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3272 3798 4823 4840 4993 5178 5623
Size: 1255 1692 526 1025 17 153 185 445
Cuts at: 5623 325
Size: 1498
Fragments arranged by size:
1692 1498 1255 1025 526 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5042 6754 96
Size: 274 80 33 51 1443 3065 1712 138
Fragments arranged by size:
3065 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2342 2374 6756 6788 2342
Size: 32 4382 32 2350
Fragments arranged by size:
4382 2350 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6796
XbaI T'CTAG_A
Cuts at: 4132 4132
Size: 6796
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2531 2011
Size: 520 6276
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6796
XmaI C'CCGG_G
Cuts at: 1870 3527 3741 4121 1870
Size: 1657 214 380 4545
Fragments arranged by size:
4545 1657 380 214
XmnI GAAnn'nnTTC
Cuts at: 3521 4925 3521
Size: 1404 5392
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4604 276
Size: 53 83 186 936 3070 2468
Fragments arranged by size:
3070 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
Bsu36I DraIII EcoNI EcoRV FspAI NruI NsiI PacI
PmlI PspXI PsrI SapI SexAI SfiI SrfI SwaI