Restriction map for
pFN21AB6273
(Circular) (Six-base) MAPSORT of: pFN21AB6273.seq Check: 8373 from: 1 to: 7042
pFN21AB6273 7042 bp
With 182 enzymes: SgfI *
February 5, 2010 17:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2660 3586 3809 2660
Size: 926 223 5893
Fragments arranged by size:
5893 926 223
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4852 278
Size: 53 83 186 936 3316 2468
Fragments arranged by size:
3316 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4385 1937
Size: 2448 4594
Acc65I G'GTAC_C
Cuts at: 4363 4363
Size: 7042
AclI AA'CG_TT
Cuts at: 1563 5169 5542 1563
Size: 3606 373 3063
Fragments arranged by size:
3606 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2181 2937 5105 6489 6757 1632
Size: 549 756 2168 1384 268 1917
Fragments arranged by size:
2168 1917 1384 756 549 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6845
AflIII A'CryG_T
Cuts at: 1203 5941 6918 1203
Size: 4738 977 1327
Fragments arranged by size:
4738 1327 977
AgeI A'CCGG_T
Cuts at: 5873 5873
Size: 7042
AhdI GACnn_n'nnGTC
Cuts at: 5771 5771
Size: 7042
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7042
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2668 1184
Size: 1484 5558
AlwNI CAG_nnn'CTG
Cuts at: 4785 6357 4785
Size: 1572 5470
ApaI G_GGCC'C
Cuts at: 3673 3673
Size: 7042
ApaLI G'TGCA_C
Cuts at: 5099 6255 5099
Size: 1156 5886
ApoI r'AATT_y
Cuts at: 1493 2850 4337 4351 4577 4729 6838 1493
Size: 1357 1487 14 226 152 2109 1697
Fragments arranged by size:
2109 1697 1487 1357 226 152 14
AseI AT'TA_AT
Cuts at: 160 1032 5596 160
Size: 872 4564 1606
Fragments arranged by size:
4564 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7042
AvaI C'yCGr_G
Cuts at: 1870 1943 4367 1870
Size: 73 2424 4545
Fragments arranged by size:
4545 2424 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4193 4226 4193
Size: 33 7009
BamHI G'GATC_C
Cuts at: 4372 4739 4372
Size: 367 6675
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3719 4363 5818 618
Size: 325 217 627 1932 644 1455 1842
Fragments arranged by size:
1932 1842 1455 644 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3659 3673 4361 1391
Size: 2268 14 688 4072
Fragments arranged by size:
4072 2268 688 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2580 3351 928
Size: 971 681 771 4619
Fragments arranged by size:
4619 971 771 681
BbvCI CC'TCA_GC
Cuts at: 2781 2786 3954 4152 2781
Size: 5 1168 198 5671
Fragments arranged by size:
5671 1168 198 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5233 5267 1903
Size: 34 50 34 3212 34 3678
Fragments arranged by size:
3678 3212 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4934 6144 4934
Size: 1210 5832
BclI T'GATC_A
Cuts at: 1548 3169 1548
Size: 1621 5421
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5653 136
Size: 107 122 71 631 605 3981 1525
Fragments arranged by size:
3981 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2755 7037 2755
Size: 4282 2760
BlpI GC'TnA_GC
Cuts at: 3292 4041 4263 4459 3292
Size: 749 222 196 5875
Fragments arranged by size:
5875 749 222 196
Bme1580I G_kGCm'C
Cuts at: 1790 2062 3673 5103 6259 1790
Size: 272 1611 1430 1156 2573
Fragments arranged by size:
2573 1611 1430 1156 272
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7042
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3786 3928 4773
Size: 416 53 83 399 24 2369 142 845
Cuts at: 4773 5731 442
Size: 958 1753
Fragments arranged by size:
2369 1753 958 845 416 399 142 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7042
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3268 3300 3546 3857 4089 5702 1239
Size: 2029 32 246 311 232 1613 2579
Fragments arranged by size:
2579 2029 1613 311 246 232 32
Bpu10I CC'TnA_GC
Cuts at: 2276 2781 2786 3418 3746 3954 4137 4152 2276
Size: 505 5 632 328 208 183 15 5166
Fragments arranged by size:
5166 632 505 328 208 183 15 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2609 3397 3798 5167 6032 6330 2609
Size: 788 401 1369 865 298 3321
Fragments arranged by size:
3321 1369 865 788 401 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2062 2297 5705 882
Size: 617 563 235 3408 2219
Fragments arranged by size:
3408 2219 617 563 235
BsaAI yAC'GTr
Cuts at: 493 1204 2441 4833 493
Size: 711 1237 2392 2702
Fragments arranged by size:
2702 2392 1237 711
BsaBI GATnn'nnATC
Cuts at: 3540 7036 3540
Size: 3496 3546
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4849 5231 5866 275
Size: 53 83 186 936 3316 382 635 1451
Fragments arranged by size:
3316 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3262 5475 5873 6147 6294 6705 3262
Size: 2213 398 274 147 411 3599
Fragments arranged by size:
3599 2213 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2881 2911 3475 3505 1178
Size: 30 165 30 1478 30 564 30 4715
Fragments arranged by size:
4715 1478 564 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2766 2769 3477 3480 4162 1186
Size: 1580 3 708 3 682 4066
Fragments arranged by size:
4066 1580 708 682 3 3
BseYI C'CCAG_C
Cuts at: 3703 6245 6741 3703
Size: 2542 496 4004
Fragments arranged by size:
4004 2542 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2058 2638 1163
Size: 573 322 580 5567
Fragments arranged by size:
5567 580 573 322
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4492 5253 6281 1540
Size: 275 186 2491 761 1028 2301
Fragments arranged by size:
2491 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5103 5188 6259 5103
Size: 85 1071 5886
Fragments arranged by size:
5886 1071 85
BsmI GAATG_Cn'
Cuts at: 4553 4646 4553
Size: 93 6949
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 3659 3673 4361 5103 5188 6259
Size: 399 272 1597 14 688 742 85 1071
Cuts at: 6259 1391
Size: 2174
Fragments arranged by size:
2174 1597 1071 742 688 399 272 85
14
BspEI T'CCGG_A
Cuts at: 6705 6705
Size: 7042
BspHI T'CATG_A
Cuts at: 4931 6661 4931
Size: 1730 5312
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2660 2819 3586 3809 4397 844
Size: 1816 159 767 223 588 3489
Fragments arranged by size:
3489 1816 767 588 223 159
BsrBI CCG'CTC
Cuts at: 1394 1823 3750 4929 1394
Size: 429 1927 1179 3507
Fragments arranged by size:
3507 1927 1179 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2413 3804 5537 5711 65
Size: 1183 1165 1391 1733 174 1396
Fragments arranged by size:
1733 1396 1391 1183 1165 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5686 5873 1700
Size: 110 3876 187 2869
Fragments arranged by size:
3876 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2533 96
Size: 2437 4605
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7042
BssSI C'ACGA_G
Cuts at: 5102 6114 5102
Size: 1012 6030
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3626 794
Size: 2832 4210
BstBI TT'CG_AA
Cuts at: 5846 5846
Size: 7042
BstEII G'GTnAC_C
Cuts at: 1184 2477 1184
Size: 1293 5749
BstXI CCAn_nnnn'nTGG
Cuts at: 2985 2985
Size: 7042
BstYI r'GATC_y
Cuts at: 1972 2732 2755 3082 3535 4372 4739 5124 5141
Size: 760 23 327 453 837 367 385 17
Cuts at: 5141 6593 6679 6691 7037 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 837 760 453 385 367 346
327 86 23 17 12
Bsu36I CC'TnA_GG
Cuts at: 3433 3763 3433
Size: 330 6712
BtgI C'CryG_G
Cuts at: 513 1065 3600 513
Size: 552 2535 3955
Fragments arranged by size:
3955 2535 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6303
Fragments arranged by size:
6303 683 56
BtsI GCAGTG_nn'
Cuts at: 2655 3785 4558 5352 5372 2655
Size: 1130 773 794 20 4325
Fragments arranged by size:
4325 1130 794 773 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7042
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7007
DraI TTT'AAA
Cuts at: 3837 4346 3837
Size: 509 6533
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6049 809
Size: 507 4733 1802
Fragments arranged by size:
4733 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2904 2987 3603
Size: 54 1425 50 275 18 1074 83 616
Cuts at: 3603 4489 5378 7007 8
Size: 886 889 1629 43
Fragments arranged by size:
1629 1425 1074 889 886 616 275 83
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4489 1537
Size: 275 2677 4090
Fragments arranged by size:
4090 2677 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4307 4972 1353
Size: 2954 665 3423
Fragments arranged by size:
3423 2954 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5629 6003 6149 387
Size: 1064 138 4040 374 146 1280
Fragments arranged by size:
4040 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2181 2937 3268 3300 3546 3857 4089
Size: 393 549 756 331 32 246 311 232
Cuts at: 4089 5105 5702 6489 6757 1239
Size: 1016 597 787 268 1524
Fragments arranged by size:
1524 1016 787 756 597 549 393 331
311 268 246 232 32
EcoO109I rG'GnC_Cy
Cuts at: 3669 3858 3669
Size: 189 6853
EcoRI G'AATT_C
Cuts at: 4351 4729 4351
Size: 378 6664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2597 2597
Size: 7042
FspI TGC'GCA
Cuts at: 5548 5548
Size: 7042
HaeII r_GCGC'y
Cuts at: 1678 6189 1678
Size: 4511 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2207 2239 2680 2712 4152
Size: 32 135 32 635 32 441 32 1440
Cuts at: 4152 4184 5683 5715 5757 5789 1373
Size: 32 1499 32 42 32 2626
Fragments arranged by size:
2626 1499 1440 635 441 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4386 4632 669
Size: 988 281 2448 246 3079
Fragments arranged by size:
3079 2448 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2013 2201 748
Size: 1265 188 5589
Fragments arranged by size:
5589 1265 188
HpaI GTT'AAC
Cuts at: 4632 4632
Size: 7042
KpnI G_GTAC'C
Cuts at: 4367 4367
Size: 7042
MfeI C'AATT_G
Cuts at: 4641 4641
Size: 7042
MluI A'CGCG_T
Cuts at: 6918 6918
Size: 7042
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2531 3506 6156 6340 1305
Size: 1226 975 2650 184 2007
Fragments arranged by size:
2650 2007 1226 975 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2906 2989 10
Size: 54 1425 343 1074 83 4063
Fragments arranged by size:
4063 1425 1074 343 83 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5000 5359 5518 518
Size: 648 3834 359 159 2042
Fragments arranged by size:
3834 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2615 2785 3566 4100 4458 5135 6283 6528
Size: 1009 170 781 534 358 677 1148 245
Cuts at: 6528 1606
Size: 2120
Fragments arranged by size:
2120 1148 1009 781 677 534 358 245
170
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6490
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7042
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7042
NotI GC'GGCC_GC
Cuts at: 4489 4489
Size: 7042
NspI r_CATG'y
Cuts at: 1132 1229 3556 4400 5945 1132
Size: 97 2327 844 1545 2229
Fragments arranged by size:
2327 2229 1545 844 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7042
PciI A'CATG_T
Cuts at: 5941 5941
Size: 7042
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3616 1108
Size: 2508 4534
PmeI GTTT'AAAC
Cuts at: 4346 4346
Size: 7042
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5111 5143 6624 6656 5111
Size: 32 1481 32 5497
Fragments arranged by size:
5497 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7042
PsiI TTA'TAA
Cuts at: 4612 4612
Size: 7042
PspOMI G'GGCC_C
Cuts at: 3669 3669
Size: 7042
PstI C_TGCA'G
Cuts at: 830 3736 3912 4394 830
Size: 2906 176 482 3478
Fragments arranged by size:
3478 2906 482 176
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7042
PvuII CAG'CTG
Cuts at: 2615 2785 3566 4100 2615
Size: 170 781 534 5557
Fragments arranged by size:
5557 781 534 170
SalI G'TCGA_C
Cuts at: 1936 4384 1936
Size: 2448 4594
SbfI CC_TGCA'GG
Cuts at: 3736 4394 3736
Size: 658 6384
ScaI AGT'ACT
Cuts at: 5290 7002 5290
Size: 1712 5330
SfcI C'TryA_G
Cuts at: 826 1045 2446 2610 2640 3732 3908 4390 5525
Size: 219 1401 164 30 1092 176 482 1135
Cuts at: 5525 6206 6397 826
Size: 681 191 1471
Fragments arranged by size:
1471 1401 1135 1092 681 482 219 191
176 164 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7042
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7042
SmaI CCC'GGG
Cuts at: 1872 4369 1872
Size: 2497 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2588 3412 3813 5146 6047 6309
Size: 197 926 645 824 401 1333 901 262
Cuts at: 6309 820
Size: 1553
Fragments arranged by size:
1553 1333 926 901 824 645 401 262
197
SnaBI TAC'GTA
Cuts at: 493 4833 493
Size: 4340 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7042
SphI G_CATG'C
Cuts at: 1132 3556 4400 1132
Size: 2424 844 3774
Fragments arranged by size:
3774 2424 844
SspI AAT'ATT
Cuts at: 5 52 2345 2407 4966 5
Size: 47 2293 62 2559 2081
Fragments arranged by size:
2559 2293 2081 62 47
StuI AGG'CCT
Cuts at: 1517 4031 1517
Size: 2514 4528
StyI C'CwwG_G
Cuts at: 513 1065 4481 513
Size: 552 3416 3074
Fragments arranged by size:
3416 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3218 3623 5069 5086 5239 5424 5869
Size: 1255 1638 405 1446 17 153 185 445
Cuts at: 5869 325
Size: 1498
Fragments arranged by size:
1638 1498 1446 1255 445 405 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2533 5288 7000
Size: 274 80 33 51 1443 556 2755 1712
Cuts at: 7000 96
Size: 138
Fragments arranged by size:
2755 1712 1443 556 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7002 7034 7002
Size: 32 7010
Tth111I GACn'n_nGTC
Cuts at: 1316 2221 1316
Size: 905 6137
XbaI T'CTAG_A
Cuts at: 4378 4378
Size: 7042
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3613 3881 3613
Size: 268 6774
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7042
XmaI C'CCGG_G
Cuts at: 1870 4367 1870
Size: 2497 4545
XmnI GAAnn'nnTTC
Cuts at: 3427 5171 3427
Size: 1744 5298
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4850 276
Size: 53 83 186 936 3316 2468
Fragments arranged by size:
3316 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI