Restriction map for
pFN21AB6271
(Circular) (Six-base) MAPSORT of: pFN21AB6271.seq Check: 6911 from: 1 to: 6715
pFN21AB6271 6715 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2896 3684 3751 2896
Size: 788 67 5860
Fragments arranged by size:
5860 788 67
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4525 278
Size: 53 83 186 936 2989 2468
Fragments arranged by size:
2989 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2372 4058 1937
Size: 435 1686 4594
Fragments arranged by size:
4594 1686 435
Acc65I G'GTAC_C
Cuts at: 4036 4036
Size: 6715
AclI AA'CG_TT
Cuts at: 1563 4842 5215 1563
Size: 3279 373 3063
Fragments arranged by size:
3279 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2276 2366 2402 2746 3254 3671 3749 4778
Size: 644 90 36 344 508 417 78 1029
Cuts at: 4778 6162 6430 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1029 644 508 417 344 268
90 78 36
AfeI AGC'GCT
Cuts at: 2812 3341 2812
Size: 529 6186
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6518
AflIII A'CryG_T
Cuts at: 1203 5614 6591 1203
Size: 4411 977 1327
Fragments arranged by size:
4411 1327 977
AgeI A'CCGG_T
Cuts at: 3522 5546 3522
Size: 2024 4691
AhdI GACnn_n'nnGTC
Cuts at: 5444 5444
Size: 6715
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6715
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6715
AlwNI CAG_nnn'CTG
Cuts at: 2254 3199 3334 4458 6030 2254
Size: 945 135 1124 1572 2939
Fragments arranged by size:
2939 1572 1124 945 135
ApaI G_GGCC'C
Cuts at: 2119 3498 3543 3830 2119
Size: 1379 45 287 5004
Fragments arranged by size:
5004 1379 287 45
ApaLI G'TGCA_C
Cuts at: 2399 2972 4772 5928 2399
Size: 573 1800 1156 3186
Fragments arranged by size:
3186 1800 1156 573
ApoI r'AATT_y
Cuts at: 1493 4024 4250 4402 6511 1493
Size: 2531 226 152 2109 1697
Fragments arranged by size:
2531 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2690 2690
Size: 6715
AseI AT'TA_AT
Cuts at: 160 1032 5269 160
Size: 872 4237 1606
Fragments arranged by size:
4237 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6715
AvaI C'yCGr_G
Cuts at: 1870 1943 2570 3174 3555 4040 1870
Size: 73 627 604 381 485 4545
Fragments arranged by size:
4545 627 604 485 381 73
BamHI G'GATC_C
Cuts at: 4045 4412 4045
Size: 367 6348
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2417 2666 2717 3749
Size: 325 217 627 249 381 249 51 1032
Cuts at: 3749 4036 5491 618
Size: 287 1455 1842
Fragments arranged by size:
1842 1455 1032 627 381 325 287 249
249 217 51
BanII G_rGCy'C
Cuts at: 1391 2054 2119 2277 2577 2801 3126 3366 3498
Size: 663 65 158 300 224 325 240 132
Cuts at: 3498 3543 3830 4034 1391
Size: 45 287 204 4072
Fragments arranged by size:
4072 663 325 300 287 240 224 204
158 132 65 45
BbeI G_GCGC'C
Cuts at: 2040 2421 3753 2040
Size: 381 1332 5002
Fragments arranged by size:
5002 1332 381
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3136 928
Size: 971 1237 4507
Fragments arranged by size:
4507 1237 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4906 4940 1903
Size: 34 2969 34 3678
Fragments arranged by size:
3678 2969 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4607 5817 4607
Size: 1210 5505
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6715
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2756 2897 2951
Size: 107 122 71 631 605 1084 141 54
Cuts at: 2951 3641 5326 136
Size: 690 1685 1525
Fragments arranged by size:
1685 1525 1084 690 631 605 141 122
107 71 54
BglII A'GATC_T
Cuts at: 6710 6710
Size: 6715
BlpI GC'TnA_GC
Cuts at: 2225 2276 4132 2225
Size: 51 1856 4808
Fragments arranged by size:
4808 1856 51
Bme1580I G_kGCm'C
Cuts at: 1790 2119 2403 2629 2671 2722 2976 3498 3543
Size: 329 284 226 42 51 254 522 45
Cuts at: 3543 3830 3967 4776 5932 1790
Size: 287 137 809 1156 2573
Fragments arranged by size:
2573 1156 809 522 329 287 284 254
226 137 51 45 42
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6715
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2613 3962 4446
Size: 416 53 83 399 24 1196 1349 484
Cuts at: 4446 5404 442
Size: 958 1753
Fragments arranged by size:
1753 1349 1196 958 484 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6715
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2800 2896 2410
Size: 390 96 6229
Fragments arranged by size:
6229 390 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2240 2288 2468 2519 2852 3221 3934
Size: 794 207 48 180 51 333 369 713
Cuts at: 3934 3963 5375 1239
Size: 29 1412 2579
Fragments arranged by size:
2579 1412 794 713 369 333 207 180
51 48 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4840 5705 6003 4840
Size: 865 298 5552
Fragments arranged by size:
5552 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2345 5378 882
Size: 617 846 3033 2219
Fragments arranged by size:
3033 2219 846 617
BsaAI yAC'GTr
Cuts at: 493 1204 3010 3079 3885 4506 493
Size: 711 1806 69 806 621 2702
Fragments arranged by size:
2702 1806 806 711 621 69
BsaBI GATnn'nnATC
Cuts at: 6709 6709
Size: 6715
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 2418 3750 4522
Size: 53 83 186 936 504 381 1332 772
Cuts at: 4522 4904 5539 275
Size: 382 635 1451
Fragments arranged by size:
1451 1332 936 772 635 504 382 381
186 83 53
BsaWI w'CCGG_w
Cuts at: 2976 3522 5148 5546 5820 5967 6378 2976
Size: 546 1626 398 274 147 411 3313
Fragments arranged by size:
3313 1626 546 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2222 2252 2405 2435 3044
Size: 30 165 30 819 30 153 30 609
Cuts at: 3044 3074 1178
Size: 30 4819
Fragments arranged by size:
4819 819 609 165 153 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2060 2276 2285 3154 3218 3272 3573
Size: 844 30 216 9 869 64 54 301
Cuts at: 3573 3896 1186
Size: 323 4005
Fragments arranged by size:
4005 869 844 323 301 216 64 54
30 9
BseYI C'CCAG_C
Cuts at: 2477 2616 2753 2919 2988 3106 3490 3779 3794
Size: 139 137 166 69 118 384 289 15
Cuts at: 3794 3823 3927 3954 5918 6414 2477
Size: 29 104 27 1964 496 2778
Fragments arranged by size:
2778 1964 496 384 289 166 139 137
118 104 69 29 27 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2692 3257 1163
Size: 573 956 565 4621
Fragments arranged by size:
4621 956 573 565
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2214 3114 3370 4165 4926 5954
Size: 275 186 213 900 256 795 761 1028
Cuts at: 5954 1540
Size: 2301
Fragments arranged by size:
2301 1028 900 795 761 275 256 213
186
BsiHKAI G_wGCw'C
Cuts at: 2277 2403 2976 3126 3366 4776 4861 5932 2277
Size: 126 573 150 240 1410 85 1071 3060
Fragments arranged by size:
3060 1410 1071 573 240 150 126 85
BsmI GAATG_Cn'
Cuts at: 4226 4319 4226
Size: 93 6622
BsmBI CGTCTCn'nnnn_
Cuts at: 2201 2201
Size: 6715
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2119 2277 2403 2577 2629 2671
Size: 399 264 65 158 126 174 52 42
Cuts at: 2671 2722 2801 2976 3126 3366 3498 3543 3830
Size: 51 79 175 150 240 132 45 287
Cuts at: 3830 3967 4034 4776 4861 5932 1391
Size: 137 67 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 287 264 240 175
174 158 150 137 132 126 85 79
67 65 52 51 45 42
BspEI T'CCGG_A
Cuts at: 6378 6378
Size: 6715
BspHI T'CATG_A
Cuts at: 2841 4604 6334 2841
Size: 1763 1730 3222
Fragments arranged by size:
3222 1763 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2253 2896 3130 3315 3684 3751 4070 844
Size: 1409 643 234 185 369 67 319 3489
Fragments arranged by size:
3489 1409 643 369 319 234 185 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2703 3060 4602 1394
Size: 429 880 357 1542 3507
Fragments arranged by size:
3507 1542 880 429 357
BsrDI GCAATG_nn'
Cuts at: 65 1248 5210 5384 65
Size: 1183 3962 174 1396
Fragments arranged by size:
3962 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2039 2298 3522 3810 4012 5359 5546
Size: 110 229 259 1224 288 202 1347 187
Cuts at: 5546 1700
Size: 2869
Fragments arranged by size:
2869 1347 1224 288 259 229 202 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6715
BssHII G'CGCG_C
Cuts at: 1925 2543 2690 1925
Size: 618 147 5950
Fragments arranged by size:
5950 618 147
BssSI C'ACGA_G
Cuts at: 3134 4775 5787 3134
Size: 1641 1012 4062
Fragments arranged by size:
4062 1641 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2467 794
Size: 1673 5042
BstBI TT'CG_AA
Cuts at: 5519 5519
Size: 6715
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6715
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2362 3696 2011
Size: 351 1334 5030
Fragments arranged by size:
5030 1334 351
BstYI r'GATC_y
Cuts at: 1972 2085 4045 4412 4797 4814 6266 6352 6364
Size: 113 1960 367 385 17 1452 86 12
Cuts at: 6364 6710 1972
Size: 346 1977
Fragments arranged by size:
1977 1960 1452 385 367 346 113 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2771 2771
Size: 6715
BtgI C'CryG_G
Cuts at: 513 1065 2054 2895 2982 3084 3636 513
Size: 552 989 841 87 102 552 3592
Fragments arranged by size:
3592 989 841 552 552 102 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3592 487
Size: 683 56 2366 3610
Fragments arranged by size:
3610 2366 683 56
BtsI GCAGTG_nn'
Cuts at: 4231 5025 5045 4231
Size: 794 20 5901
Fragments arranged by size:
5901 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6715
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6680
DraI TTT'AAA
Cuts at: 4019 4019
Size: 6715
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3599 5722 809
Size: 507 2283 2123 1802
Fragments arranged by size:
2283 2123 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2095 2296 3348
Size: 54 1425 50 275 18 265 201 1052
Cuts at: 3348 4010 4162 5051 6680 8
Size: 662 152 889 1629 43
Fragments arranged by size:
1629 1425 1052 889 662 275 265 201
152 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4162 1537
Size: 275 2350 4090
Fragments arranged by size:
4090 2350 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2582 2660 4645 1353
Size: 1229 78 1985 3423
Fragments arranged by size:
3423 1985 1229 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3972 5302 5676 5822 387
Size: 1064 138 2383 1330 374 146 1280
Fragments arranged by size:
2383 1330 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2275 3124 3364 2275
Size: 849 240 5626
Fragments arranged by size:
5626 849 240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2240 2276 2288 2366 2402 2468
Size: 393 401 207 36 12 78 36 66
Cuts at: 2468 2519 2746 2852 3221 3254 3671 3749 3934
Size: 51 227 106 369 33 417 78 185
Cuts at: 3934 3963 4778 5375 6162 6430 1239
Size: 29 815 597 787 268 1524
Fragments arranged by size:
1524 815 787 597 417 401 393 369
268 227 207 185 106 78 78 66
51 36 36 33 29 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2459 2743 2933 3251 3296 3458 3653 2459
Size: 284 190 318 45 162 195 5521
Fragments arranged by size:
5521 318 284 195 190 162 45
EcoO109I rG'GnC_Cy
Cuts at: 2115 2452 2608 2757 2875 3179 3400 3470 3494
Size: 337 156 149 118 304 221 70 24
Cuts at: 3494 3495 3539 3827 2115
Size: 1 44 288 5003
Fragments arranged by size:
5003 337 304 288 221 156 149 118
70 44 24 1
EcoRI G'AATT_C
Cuts at: 4024 4402 4024
Size: 378 6337
FspI TGC'GCA
Cuts at: 2827 5221 2827
Size: 2394 4321
HaeII r_GCGC'y
Cuts at: 1678 2040 2166 2421 2814 3266 3343 3753 5862
Size: 362 126 255 393 452 77 410 2109
Cuts at: 5862 1678
Size: 2531
Fragments arranged by size:
2531 2109 452 410 393 362 255 126
77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2196 2228 2405 2437 5356
Size: 32 135 32 624 32 177 32 2919
Cuts at: 5356 5388 5430 5462 1373
Size: 32 42 32 2626
Fragments arranged by size:
2919 2626 624 177 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2236 4059 4305 669
Size: 988 281 298 1823 246 3079
Fragments arranged by size:
3079 1823 988 298 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6715
HpaI GTT'AAC
Cuts at: 4305 4305
Size: 6715
KasI G'GCGC_C
Cuts at: 2036 2417 3749 2036
Size: 381 1332 5002
Fragments arranged by size:
5002 1332 381
KpnI G_GTAC'C
Cuts at: 4040 4040
Size: 6715
MfeI C'AATT_G
Cuts at: 4314 4314
Size: 6715
MluI A'CGCG_T
Cuts at: 6591 6591
Size: 6715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2265 5829 6013 1305
Size: 960 3564 184 2007
Fragments arranged by size:
3564 2007 960 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3350 10
Size: 54 1425 343 1518 3375
Fragments arranged by size:
3375 1518 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3694 3883 4673 5032 5191 518
Size: 648 2528 189 790 359 159 2042
Fragments arranged by size:
2528 2042 790 648 359 189 159
MspA1I CmG'CkG
Cuts at: 1606 2056 2101 2251 2280 2380 2527 2753 2923
Size: 450 45 150 29 100 147 226 170
Cuts at: 2923 3638 4131 4808 5956 6201 1606
Size: 715 493 677 1148 245 2120
Fragments arranged by size:
2120 1148 715 677 493 450 245 226
170 150 147 100 45 29
NaeI GCC'GGC
Cuts at: 2041 2300 3812 2041
Size: 259 1512 4944
Fragments arranged by size:
4944 1512 259
NarI GG'CG_CC
Cuts at: 2037 2418 3750 2037
Size: 381 1332 5002
Fragments arranged by size:
5002 1332 381
NcoI C'CATG_G
Cuts at: 513 1065 2895 2982 3084 513
Size: 552 1830 87 102 4144
Fragments arranged by size:
4144 1830 552 102 87
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6715
NgoMIV G'CCGG_C
Cuts at: 2039 2298 3810 2039
Size: 259 1512 4944
Fragments arranged by size:
4944 1512 259
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6715
NotI GC'GGCC_GC
Cuts at: 4162 4162
Size: 6715
NspI r_CATG'y
Cuts at: 1132 1229 3041 4073 5618 1132
Size: 97 1812 1032 1545 2229
Fragments arranged by size:
2229 1812 1545 1032 97
PasI CC'CwG_GG
Cuts at: 1348 2338 3460 3831 4004 1348
Size: 990 1122 371 173 4059
Fragments arranged by size:
4059 1122 990 371 173
PciI A'CATG_T
Cuts at: 5614 5614
Size: 6715
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3490 1108
Size: 2382 4333
PfoI T'CCnGG_A
Cuts at: 2010 2535 3020 2010
Size: 525 485 5705
Fragments arranged by size:
5705 525 485
PmeI GTTT'AAAC
Cuts at: 4019 4019
Size: 6715
PmlI CAC'GTG
Cuts at: 3885 3885
Size: 6715
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4784 4816 6297 6329 4784
Size: 32 1481 32 5170
Fragments arranged by size:
5170 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2608 2608
Size: 6715
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6715
PsiI TTA'TAA
Cuts at: 4285 4285
Size: 6715
PspOMI G'GGCC_C
Cuts at: 2115 3494 3539 3826 2115
Size: 1379 45 287 5004
Fragments arranged by size:
5004 1379 287 45
PstI C_TGCA'G
Cuts at: 830 2064 2106 2151 2250 3153 3339 3762 4067
Size: 1234 42 45 99 903 186 423 305
Cuts at: 4067 830
Size: 3478
Fragments arranged by size:
3478 1234 903 423 305 186 99 45
42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6715
PvuII CAG'CTG
Cuts at: 2101 2251 2527 2923 2101
Size: 150 276 396 5893
Fragments arranged by size:
5893 396 276 150
SacI G_AGCT'C
Cuts at: 2277 3126 3366 2277
Size: 849 240 5626
Fragments arranged by size:
5626 849 240
SacII CC_GC'GG
Cuts at: 2057 3639 2057
Size: 1582 5133
SalI G'TCGA_C
Cuts at: 1936 4057 1936
Size: 2121 4594
SanDI GG'GwC_CC
Cuts at: 2608 2608
Size: 6715
SapI GCTCTTCn'nnn_
Cuts at: 2582 2582
Size: 6715
SbfI CC_TGCA'GG
Cuts at: 3762 4067 3762
Size: 305 6410
ScaI AGT'ACT
Cuts at: 3053 4963 6675 3053
Size: 1910 1712 3093
Fragments arranged by size:
3093 1910 1712
SfcI C'TryA_G
Cuts at: 826 1045 2060 2102 2147 2176 2246 3149 3335
Size: 219 1015 42 45 29 70 903 186
Cuts at: 3335 3758 4063 5198 5879 6070 826
Size: 423 305 1135 681 191 1471
Fragments arranged by size:
1471 1135 1015 903 681 423 305 219
191 186 70 45 42 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 2756 2756
Size: 6715
SfoI GGC'GCC
Cuts at: 2038 2419 3751 2038
Size: 381 1332 5002
Fragments arranged by size:
5002 1332 381
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6715
SgrAI Cr'CCGG_yG
Cuts at: 1700 2039 1700
Size: 339 6376
SmaI CCC'GGG
Cuts at: 1872 4042 1872
Size: 2170 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4819 5720 5982 820
Size: 197 926 2876 901 262 1553
Fragments arranged by size:
2876 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4506 493
Size: 4013 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6715
SphI G_CATG'C
Cuts at: 1132 4073 1132
Size: 2941 3774
SspI AAT'ATT
Cuts at: 5 52 4639 5
Size: 47 4587 2081
Fragments arranged by size:
4587 2081 47
StuI AGG'CCT
Cuts at: 1517 2738 1517
Size: 1221 5494
StyI C'CwwG_G
Cuts at: 513 1065 2355 2895 2982 3084 4154 513
Size: 552 1290 540 87 102 1070 3074
Fragments arranged by size:
3074 1290 1070 552 540 102 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3128 4017 4742 4759 4912 5097 5542
Size: 1255 1548 889 725 17 153 185 445
Cuts at: 5542 325
Size: 1498
Fragments arranged by size:
1548 1498 1255 889 725 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2961 3051 4961
Size: 274 80 33 51 1443 984 90 1910
Cuts at: 4961 6673 96
Size: 1712 138
Fragments arranged by size:
1910 1712 1443 984 274 138 90 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6675 6707 6675
Size: 32 6683
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6715
XbaI T'CTAG_A
Cuts at: 3785 4051 3785
Size: 266 6449
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2335 2681 2750 3696 3760 3857 2335
Size: 346 69 946 64 97 5193
Fragments arranged by size:
5193 946 346 97 69 64
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6715
XmaI C'CCGG_G
Cuts at: 1870 4040 1870
Size: 2170 4545
XmnI GAAnn'nnTTC
Cuts at: 4844 4844
Size: 6715
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4523 276
Size: 53 83 186 936 2989 2468
Fragments arranged by size:
2989 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I
DraIII EcoRV FalI FseI FspAI NruI NsiI PacI
PspXI PsrI RsrII SexAI SrfI SwaI