Restriction map for
pFN21AB6269
(Circular) (Six-base) MAPSORT of: pFN21AB6269.seq Check: 8891 from: 1 to: 6787
pFN21AB6269 6787 bp
With 182 enzymes: SgfI *
March 28, 2012 12:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4597 278
Size: 53 83 186 936 3061 2468
Fragments arranged by size:
3061 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2088 4130 1937
Size: 151 2042 4594
Fragments arranged by size:
4594 2042 151
Acc65I G'GTAC_C
Cuts at: 4108 4108
Size: 6787
AclI AA'CG_TT
Cuts at: 1563 4914 5287 1563
Size: 3351 373 3063
Fragments arranged by size:
3351 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2036 2657 2880 2902 4850 6234 6502 1632
Size: 404 621 223 22 1948 1384 268 1917
Fragments arranged by size:
1948 1917 1384 621 404 268 223 22
AflII C'TTAA_G
Cuts at: 820 1017 3863 820
Size: 197 2846 3744
Fragments arranged by size:
3744 2846 197
AflIII A'CryG_T
Cuts at: 1203 3481 5686 6663 1203
Size: 2278 2205 977 1327
Fragments arranged by size:
2278 2205 1327 977
AgeI A'CCGG_T
Cuts at: 3103 5618 3103
Size: 2515 4272
AhdI GACnn_n'nnGTC
Cuts at: 5516 5516
Size: 6787
AleI CACnn'nnGTG
Cuts at: 1166 2324 1166
Size: 1158 5629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6787
AlwNI CAG_nnn'CTG
Cuts at: 3254 3916 4530 6102 3254
Size: 662 614 1572 3939
Fragments arranged by size:
3939 1572 662 614
ApaLI G'TGCA_C
Cuts at: 2342 4844 6000 2342
Size: 2502 1156 3129
Fragments arranged by size:
3129 2502 1156
ApoI r'AATT_y
Cuts at: 1493 2827 2890 3040 3817 4096 4322 4474 6583
Size: 1334 63 150 777 279 226 152 2109
Cuts at: 6583 1493
Size: 1697
Fragments arranged by size:
2109 1697 1334 777 279 226 152 150
63
AseI AT'TA_AT
Cuts at: 160 1032 5341 160
Size: 872 4309 1606
Fragments arranged by size:
4309 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6787
AvaI C'yCGr_G
Cuts at: 1870 1943 2197 2264 2297 2475 4112 1870
Size: 73 254 67 33 178 1637 4545
Fragments arranged by size:
4545 1637 254 178 73 67 33
BamHI G'GATC_C
Cuts at: 4117 4484 4117
Size: 367 6420
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2390 2423 2521 2545 4108
Size: 325 217 627 603 33 98 24 1563
Cuts at: 4108 5563 618
Size: 1455 1842
Fragments arranged by size:
1842 1563 1455 627 603 325 217 98
33 24
BanII G_rGCy'C
Cuts at: 1391 2082 4106 1391
Size: 691 2024 4072
Fragments arranged by size:
4072 2024 691
BbeI G_GCGC'C
Cuts at: 2394 2427 2394
Size: 33 6754
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2799 3210 3597 928
Size: 971 900 411 387 4118
Fragments arranged by size:
4118 971 900 411 387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3770 3804 4978 5012 1903
Size: 34 1833 34 1174 34 3678
Fragments arranged by size:
3678 1833 1174 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4679 5889 4679
Size: 1210 5577
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6787
BfrBI ATG'CAT
Cuts at: 2333 2333
Size: 6787
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5398 136
Size: 107 122 71 631 605 3726 1525
Fragments arranged by size:
3726 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2095 3466 6782 2095
Size: 1371 3316 2100
Fragments arranged by size:
3316 2100 1371
BlpI GC'TnA_GC
Cuts at: 2066 2180 4204 2066
Size: 114 2024 4649
Fragments arranged by size:
4649 2024 114
Bme1580I G_kGCm'C
Cuts at: 1790 2346 4848 6004 1790
Size: 556 2502 1156 2573
Fragments arranged by size:
2573 2502 1156 556
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6787
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2728 3414 4518
Size: 416 53 83 399 24 1311 686 1104
Cuts at: 4518 5476 442
Size: 958 1753
Fragments arranged by size:
1753 1311 1104 958 686 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6787
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2860 2860
Size: 6787
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2855 3980 5447 1239
Size: 1616 1125 1467 2579
Fragments arranged by size:
2579 1616 1467 1125
Bpu10I CC'TnA_GC
Cuts at: 2541 3060 2541
Size: 519 6268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3628 4912 5777 6075 3628
Size: 1284 865 298 4340
Fragments arranged by size:
4340 1284 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5450 882
Size: 617 3951 2219
Fragments arranged by size:
3951 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3484 4578 493
Size: 711 2280 1094 2702
Fragments arranged by size:
2702 2280 1094 711
BsaBI GATnn'nnATC
Cuts at: 2943 6781 2943
Size: 3838 2949
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2391 2424 2502 4594
Size: 53 83 186 936 858 33 78 2092
Cuts at: 4594 4976 5611 275
Size: 382 635 1451
Fragments arranged by size:
2092 1451 936 858 635 382 186 83
78 53 33
BsaWI w'CCGG_w
Cuts at: 2411 3103 5220 5618 5892 6039 6450 2411
Size: 692 2117 398 274 147 411 2748
Fragments arranged by size:
2748 2117 692 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6562
Fragments arranged by size:
6562 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2430 2528 2588 1186
Size: 1244 98 60 5385
Fragments arranged by size:
5385 1244 98 60
BseYI C'CCAG_C
Cuts at: 2622 5990 6486 2622
Size: 3368 496 2923
Fragments arranged by size:
3368 2923 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 2474 3504 1163
Size: 573 297 441 1030 4446
Fragments arranged by size:
4446 1030 573 441 297
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 4237 4998 6026 1540
Size: 275 186 33 2203 761 1028 2301
Fragments arranged by size:
2301 2203 1028 761 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 2346 4848 4933 6004 2346
Size: 2502 85 1071 3129
Fragments arranged by size:
3129 2502 1071 85
BsmI GAATG_Cn'
Cuts at: 4298 4391 4298
Size: 93 6694
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2346 4106 4848 4933 6004 1391
Size: 399 292 264 1760 742 85 1071 2174
Fragments arranged by size:
2174 1760 1071 742 399 292 264 85
BspEI T'CCGG_A
Cuts at: 6450 6450
Size: 6787
BspHI T'CATG_A
Cuts at: 2950 3379 3931 4676 6406 2950
Size: 429 552 745 1730 3331
Fragments arranged by size:
3331 1730 745 552 429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 4142 844
Size: 1268 2030 3489
Fragments arranged by size:
3489 2030 1268
BsrBI CCG'CTC
Cuts at: 1394 1823 2480 4674 1394
Size: 429 657 2194 3507
Fragments arranged by size:
3507 2194 657 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5282 5456 65
Size: 1183 4034 174 1396
Fragments arranged by size:
4034 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2033 2420 3103 5431 5618 1700
Size: 110 223 387 683 2328 187 2869
Fragments arranged by size:
2869 2328 683 387 223 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6787
BssHII G'CGCG_C
Cuts at: 1925 2212 2290 2292 1925
Size: 287 78 2 6420
Fragments arranged by size:
6420 287 78 2
BssSI C'ACGA_G
Cuts at: 4847 5859 4847
Size: 1012 5775
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6787
BstBI TT'CG_AA
Cuts at: 5591 5591
Size: 6787
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6787
BstXI CCAn_nnnn'nTGG
Cuts at: 4039 4039
Size: 6787
BstYI r'GATC_y
Cuts at: 1972 2095 2992 3466 4117 4484 4869 4886 6338
Size: 123 897 474 651 367 385 17 1452
Cuts at: 6338 6424 6436 6782 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 897 651 474 385 367 346
123 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4048 4048
Size: 6787
BtgI C'CryG_G
Cuts at: 513 1065 2377 2526 3460 3548 513
Size: 552 1312 149 934 88 3752
Fragments arranged by size:
3752 1312 934 552 149 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6048
Fragments arranged by size:
6048 683 56
BtsI GCAGTG_nn'
Cuts at: 4303 5097 5117 4303
Size: 794 20 5973
Fragments arranged by size:
5973 794 20
ClaI AT'CG_AT
Cuts at: 1554 3222 1554
Size: 1668 5119
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6752
DraI TTT'AAA
Cuts at: 4091 4091
Size: 6787
DraIII CAC_nnn'GTG
Cuts at: 2350 3325 2350
Size: 975 5812
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3013 3728 5794 809
Size: 507 1697 715 2066 1802
Fragments arranged by size:
2066 1802 1697 715 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2160 3195
Size: 54 1425 50 275 18 201 129 1035
Cuts at: 3195 4234 5123 6752 8
Size: 1039 889 1629 43
Fragments arranged by size:
1629 1425 1039 1035 889 275 201 129
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2031 4234 1537
Size: 275 219 2203 4090
Fragments arranged by size:
4090 2203 275 219
EarI CTCTTCn'nnn_
Cuts at: 1353 2505 2868 2983 3751 4717 1353
Size: 1152 363 115 768 966 3423
Fragments arranged by size:
3423 1152 966 768 363 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2158 2574 5374 5748 5894 387
Size: 1064 138 569 416 2800 374 146 1280
Fragments arranged by size:
2800 1280 1064 569 416 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2657 2855 2880 2902 3980 4850
Size: 393 404 621 198 25 22 1078 870
Cuts at: 4850 5447 6234 6502 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1078 870 787 621 597 404 393
268 198 25 22
EcoNI CCTnn'n_nnAGG
Cuts at: 2129 2865 2129
Size: 736 6051
EcoO109I rG'GnC_Cy
Cuts at: 2302 2470 2302
Size: 168 6619
EcoRI G'AATT_C
Cuts at: 2890 4096 4474 2890
Size: 1206 378 5203
Fragments arranged by size:
5203 1206 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3752 3752
Size: 6787
FspI TGC'GCA
Cuts at: 3780 5293 3780
Size: 1513 5274
HaeII r_GCGC'y
Cuts at: 1678 2394 2427 5934 1678
Size: 716 33 3507 2531
Fragments arranged by size:
3507 2531 716 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3350 3382 5428 5460 5502
Size: 32 135 32 1778 32 2046 32 42
Cuts at: 5502 5534 1373
Size: 32 2626
Fragments arranged by size:
2626 2046 1778 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3109 4131 4377 669
Size: 988 281 1171 1022 246 3079
Fragments arranged by size:
3079 1171 1022 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3425 3633 748
Size: 2677 208 3902
Fragments arranged by size:
3902 2677 208
HpaI GTT'AAC
Cuts at: 4377 4377
Size: 6787
KasI G'GCGC_C
Cuts at: 2390 2423 2390
Size: 33 6754
KpnI G_GTAC'C
Cuts at: 4112 4112
Size: 6787
MfeI C'AATT_G
Cuts at: 4386 4386
Size: 6787
MluI A'CGCG_T
Cuts at: 6663 6663
Size: 6787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3020 5901 6085 1305
Size: 1715 2881 184 2007
Fragments arranged by size:
2881 2007 1715 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3197 10
Size: 54 1425 343 1365 3600
Fragments arranged by size:
3600 1425 1365 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2324 2763 2907 4745 5104 5263 518
Size: 648 1158 439 144 1838 359 159 2042
Fragments arranged by size:
2042 1838 1158 648 439 359 159 144
MspA1I CmG'CkG
Cuts at: 1606 2275 2528 4203 4880 6028 6273 1606
Size: 669 253 1675 677 1148 245 2120
Fragments arranged by size:
2120 1675 1148 677 669 253 245
NaeI GCC'GGC
Cuts at: 2035 2422 2035
Size: 387 6400
NarI GG'CG_CC
Cuts at: 2391 2424 2391
Size: 33 6754
NcoI C'CATG_G
Cuts at: 513 1065 3460 513
Size: 552 2395 3840
Fragments arranged by size:
3840 2395 552
NdeI CA'TA_TG
Cuts at: 387 3117 387
Size: 2730 4057
NgoMIV G'CCGG_C
Cuts at: 2033 2420 2033
Size: 387 6400
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6787
NotI GC'GGCC_GC
Cuts at: 4234 4234
Size: 6787
NsiI A_TGCA'T
Cuts at: 2335 2335
Size: 6787
NspI r_CATG'y
Cuts at: 1132 1229 3676 3700 4145 5690 1132
Size: 97 2447 24 445 1545 2229
Fragments arranged by size:
2447 2229 1545 445 97 24
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6787
PciI A'CATG_T
Cuts at: 5686 5686
Size: 6787
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6787
PfoI T'CCnGG_A
Cuts at: 2280 3073 2280
Size: 793 5994
PmeI GTTT'AAAC
Cuts at: 4091 4091
Size: 6787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2129 2161 4856 4888 6369 6401 2129
Size: 32 2695 32 1481 32 2515
Fragments arranged by size:
2695 2515 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2302 2470 2302
Size: 168 6619
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6787
PsiI TTA'TAA
Cuts at: 4357 4357
Size: 6787
PstI C_TGCA'G
Cuts at: 830 2133 3036 4139 830
Size: 1303 903 1103 3478
Fragments arranged by size:
3478 1303 1103 903
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6787
PvuII CAG'CTG
Cuts at: 2275 2275
Size: 6787
SacII CC_GC'GG
Cuts at: 2529 2529
Size: 6787
SalI G'TCGA_C
Cuts at: 1936 4129 1936
Size: 2193 4594
SanDI GG'GwC_CC
Cuts at: 2302 2470 2302
Size: 168 6619
SbfI CC_TGCA'GG
Cuts at: 4139 4139
Size: 6787
ScaI AGT'ACT
Cuts at: 5035 6747 5035
Size: 1712 5075
SfcI C'TryA_G
Cuts at: 826 1045 2129 3032 3342 3396 3654 4135 5270
Size: 219 1084 903 310 54 258 481 1135
Cuts at: 5270 5951 6142 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1084 903 681 481 310 258
219 191 54
SfoI GGC'GCC
Cuts at: 2392 2425 2392
Size: 33 6754
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6787
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6787
SmaI CCC'GGG
Cuts at: 1872 4114 1872
Size: 2242 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3607 3863 4891 5792 6054 820
Size: 197 926 1664 256 1028 901 262 1553
Fragments arranged by size:
1664 1553 1028 926 901 262 256 197
SnaBI TAC'GTA
Cuts at: 493 4578 493
Size: 4085 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6787
SphI G_CATG'C
Cuts at: 1132 3676 4145 1132
Size: 2544 469 3774
Fragments arranged by size:
3774 2544 469
SspI AAT'ATT
Cuts at: 5 52 4711 5
Size: 47 4659 2081
Fragments arranged by size:
4659 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6787
StyI C'CwwG_G
Cuts at: 513 1065 2232 2496 3460 3961 4226 513
Size: 552 1167 264 964 501 265 3074
Fragments arranged by size:
3074 1167 964 552 501 265 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4814 4831 4984 5169 5614 325
Size: 1255 3234 17 153 185 445 1498
Fragments arranged by size:
3234 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5033 6745 96
Size: 274 80 33 51 1443 3056 1712 138
Fragments arranged by size:
3056 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6747 6779 6747
Size: 32 6755
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6787
XbaI T'CTAG_A
Cuts at: 2744 4123 2744
Size: 1379 5408
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4039 4039
Size: 6787
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6787
XmaI C'CCGG_G
Cuts at: 1870 4112 1870
Size: 2242 4545
XmnI GAAnn'nnTTC
Cuts at: 3027 3543 4916 3027
Size: 516 1373 4898
Fragments arranged by size:
4898 1373 516
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4595 276
Size: 53 83 186 936 3061 2468
Fragments arranged by size:
3061 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI NruI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SapI
SexAI SfiI SrfI SwaI