Restriction map for
pFN21AB6268
(Circular) (Six-base) MAPSORT of: pFN21AB6268.seq Check: 2286 from: 1 to: 7021
pFN21AB6268 7021 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3598 3598
Size: 7021
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4032 4831 278
Size: 53 83 186 936 2496 799 2468
Fragments arranged by size:
2496 2468 936 799 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2124 2577 4364 1937
Size: 187 453 1787 4594
Fragments arranged by size:
4594 1787 453 187
Acc65I G'GTAC_C
Cuts at: 4342 4342
Size: 7021
AclI AA'CG_TT
Cuts at: 1563 5148 5521 1563
Size: 3585 373 3063
Fragments arranged by size:
3585 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2487 2949 2951 3267 3887 5084 6468 6736
Size: 855 462 2 316 620 1197 1384 268
Cuts at: 6736 1632
Size: 1917
Fragments arranged by size:
1917 1384 1197 855 620 462 316 268
2
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6824
AflIII A'CryG_T
Cuts at: 1203 5920 6897 1203
Size: 4717 977 1327
Fragments arranged by size:
4717 1327 977
AgeI A'CCGG_T
Cuts at: 5852 5852
Size: 7021
AhdI GACnn_n'nnGTC
Cuts at: 2512 5750 2512
Size: 3238 3783
AleI CACnn'nnGTG
Cuts at: 1166 2694 1166
Size: 1528 5493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7021
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4295 4327 4295
Size: 32 6989
AlwNI CAG_nnn'CTG
Cuts at: 2510 4764 6336 2510
Size: 2254 1572 3195
Fragments arranged by size:
3195 2254 1572
ApaLI G'TGCA_C
Cuts at: 5078 6234 5078
Size: 1156 5865
ApoI r'AATT_y
Cuts at: 1493 3200 3595 4330 4556 4708 6817 1493
Size: 1707 395 735 226 152 2109 1697
Fragments arranged by size:
2109 1707 1697 735 395 226 152
AscI GG'CGCG_CC
Cuts at: 2048 2048
Size: 7021
AseI AT'TA_AT
Cuts at: 160 1032 3800 5575 160
Size: 872 2768 1775 1606
Fragments arranged by size:
2768 1775 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7021
AvaI C'yCGr_G
Cuts at: 1870 1943 2298 2420 2669 3506 3912 4346 1870
Size: 73 355 122 249 837 406 434 4545
Fragments arranged by size:
4545 837 434 406 355 249 122 73
AvrII C'CTAG_G
Cuts at: 4241 4241
Size: 7021
BamHI G'GATC_C
Cuts at: 4351 4718 4351
Size: 367 6654
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3430 4207 4342 5797 618
Size: 325 217 627 1643 777 135 1455 1842
Fragments arranged by size:
1842 1643 1455 777 627 325 217 135
BanII G_rGCy'C
Cuts at: 1391 2421 2613 3164 4340 1391
Size: 1030 192 551 1176 4072
Fragments arranged by size:
4072 1176 1030 551 192
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2508 928
Size: 971 609 5441
Fragments arranged by size:
5441 971 609
BbvCI CC'TCA_GC
Cuts at: 3236 3236
Size: 7021
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5212 5246 1903
Size: 34 3275 34 3678
Fragments arranged by size:
3678 3275 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4913 6123 4913
Size: 1210 5811
BclI T'GATC_A
Cuts at: 1548 3763 1548
Size: 2215 4806
BfrBI ATG'CAT
Cuts at: 3669 3669
Size: 7021
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4205 5632
Size: 107 122 71 631 605 337 2196 1427
Cuts at: 5632 136
Size: 1525
Fragments arranged by size:
2196 1525 1427 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 7016 7016
Size: 7021
BlpI GC'TnA_GC
Cuts at: 2250 4438 2250
Size: 2188 4833
Bme1580I G_kGCm'C
Cuts at: 1790 4201 5082 6238 1790
Size: 2411 881 1156 2573
Fragments arranged by size:
2573 2411 1156 881
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7021
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2151 2209 3167
Size: 416 53 83 399 24 734 58 958
Cuts at: 3167 3591 4752 5710 442
Size: 424 1161 958 1753
Fragments arranged by size:
1753 1161 958 958 734 424 416 399
83 58 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7021
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2809 2809
Size: 7021
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2615 3102 3423 5681 1239
Size: 1376 487 321 2258 2579
Fragments arranged by size:
2579 2258 1376 487 321
Bpu10I CC'TnA_GC
Cuts at: 3236 3236
Size: 7021
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5146 6011 6309 5146
Size: 865 298 5858
Fragments arranged by size:
5858 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2419 2991 3063 5684 882
Size: 617 920 572 72 2621 2219
Fragments arranged by size:
2621 2219 920 617 572 72
BsaAI yAC'GTr
Cuts at: 493 1204 4812 493
Size: 711 3608 2702
Fragments arranged by size:
3608 2702 711
BsaBI GATnn'nnATC
Cuts at: 7015 7015
Size: 7021
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3909 4029 4828 5210
Size: 53 83 186 936 2376 120 799 382
Cuts at: 5210 5845 275
Size: 635 1451
Fragments arranged by size:
2376 1451 936 799 635 382 186 120
83 53
BsaWI w'CCGG_w
Cuts at: 4032 5454 5852 6126 6273 6684 4032
Size: 1422 398 274 147 411 4369
Fragments arranged by size:
4369 1422 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4056 4086 4295 4325 1178
Size: 30 165 30 2653 30 209 30 3874
Fragments arranged by size:
3874 2653 209 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 2336 2470 2612 2852 3194 3729 4051
Size: 1147 3 134 142 240 342 535 322
Cuts at: 4051 4077 4178 1186
Size: 26 101 4029
Fragments arranged by size:
4029 1147 535 342 322 240 142 134
101 26 3
BseYI C'CCAG_C
Cuts at: 4154 6224 6720 4154
Size: 2070 496 4455
Fragments arranged by size:
4455 2070 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6448
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4471 5232 6260 1540
Size: 275 186 2470 761 1028 2301
Fragments arranged by size:
2470 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2421 2739 5082 5167 6238 2421
Size: 318 2343 85 1071 3204
Fragments arranged by size:
3204 2343 1071 318 85
BsmI GAATG_Cn'
Cuts at: 4532 4625 4532
Size: 93 6928
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2421 2613 2739 3164 4201 4340 5082
Size: 399 631 192 126 425 1037 139 742
Cuts at: 5082 5167 6238 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1037 742 631 425 399 192
139 126 85
BspEI T'CCGG_A
Cuts at: 4032 6684 4032
Size: 2652 4369
BspHI T'CATG_A
Cuts at: 2449 4910 6640 2449
Size: 2461 1730 2830
Fragments arranged by size:
2830 2461 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3598 3903 4376 844
Size: 2754 305 473 3489
Fragments arranged by size:
3489 2754 473 305
BsrBI CCG'CTC
Cuts at: 1394 1823 4908 1394
Size: 429 3085 3507
Fragments arranged by size:
3507 3085 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2656 3671 5516 5690 65
Size: 1183 1408 1015 1845 174 1396
Fragments arranged by size:
1845 1408 1396 1183 1015 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3397 4168 5665 5852 1700
Size: 110 1587 771 1497 187 2869
Fragments arranged by size:
2869 1587 1497 771 187 110
BsrGI T'GTAC_A
Cuts at: 96 3755 96
Size: 3659 3362
BssHII G'CGCG_C
Cuts at: 1925 2048 1925
Size: 123 6898
BssSI C'ACGA_G
Cuts at: 2177 4023 5081 6093 2177
Size: 1846 1058 1012 3105
Fragments arranged by size:
3105 1846 1058 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7021
BstBI TT'CG_AA
Cuts at: 5825 5825
Size: 7021
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7021
BstXI CCAn_nnnn'nTGG
Cuts at: 2633 2633
Size: 7021
BstYI r'GATC_y
Cuts at: 1972 2588 4351 4718 5103 5120 6572 6658 6670
Size: 616 1763 367 385 17 1452 86 12
Cuts at: 6670 7016 1972
Size: 346 1977
Fragments arranged by size:
1977 1763 1452 616 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2857 4161 2857
Size: 1304 5717
BtgI C'CryG_G
Cuts at: 513 1065 2004 2358 2704 2766 3288 4110 513
Size: 552 939 354 346 62 522 822 3424
Fragments arranged by size:
3424 939 822 552 522 354 346 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6282
Fragments arranged by size:
6282 683 56
BtsI GCAGTG_nn'
Cuts at: 3494 4201 4537 5331 5351 3494
Size: 707 336 794 20 5164
Fragments arranged by size:
5164 794 707 336 20
ClaI AT'CG_AT
Cuts at: 1554 3693 1554
Size: 2139 4882
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3277 3312 529
Size: 35 2713 35 4238
Fragments arranged by size:
4238 2713 35 35
DraI TTT'AAA
Cuts at: 4325 4325
Size: 7021
DraIII CAC_nnn'GTG
Cuts at: 2437 4025 2437
Size: 1588 5433
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6028 809
Size: 507 4712 1802
Fragments arranged by size:
4712 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2527 4011
Size: 54 1425 50 275 18 225 472 1484
Cuts at: 4011 4278 4468 5357 6986 8
Size: 267 190 889 1629 43
Fragments arranged by size:
1629 1484 1425 889 472 275 267 225
190 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4468 1537
Size: 275 2656 4090
Fragments arranged by size:
4090 2656 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2270 2506 3553 3861 4951 1353
Size: 917 236 1047 308 1090 3423
Fragments arranged by size:
3423 1090 1047 917 308 236
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 4120 5608 5982 6128 387
Size: 1064 138 438 2093 1488 374 146 1280
Fragments arranged by size:
2093 1488 1280 1064 438 374 146 138
EcoICRI GAG'CTC
Cuts at: 2419 2419
Size: 7021
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2487 2615 2949 2951 3102 3267 3423
Size: 393 855 128 334 2 151 165 156
Cuts at: 3423 3887 5084 5681 6468 6736 1239
Size: 464 1197 597 787 268 1524
Fragments arranged by size:
1524 1197 855 787 597 464 393 334
268 165 156 151 128 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2312 2597 2637 2312
Size: 285 40 6696
Fragments arranged by size:
6696 285 40
EcoO109I rG'GnC_Cy
Cuts at: 2203 2824 2203
Size: 621 6400
EcoRI G'AATT_C
Cuts at: 3200 3595 4330 4708 3200
Size: 395 735 378 5513
Fragments arranged by size:
5513 735 395 378
EcoRV GAT'ATC
Cuts at: 3516 3516
Size: 7021
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4012 4012
Size: 7021
FspI TGC'GCA
Cuts at: 5527 5527
Size: 7021
HaeII r_GCGC'y
Cuts at: 1678 2024 2296 6168 1678
Size: 346 272 3872 2531
Fragments arranged by size:
3872 2531 346 272
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2498 2530 2726 2758 3137
Size: 32 135 32 926 32 196 32 379
Cuts at: 3137 3169 3500 3532 3860 3892 4056 4088 5662
Size: 32 331 32 328 32 164 32 1574
Cuts at: 5662 5694 5736 5768 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1574 926 379 331 328 196 164
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2578 4003 4365 4611 669
Size: 988 281 640 1425 362 246 3079
Fragments arranged by size:
3079 1425 988 640 362 281 246
HindIII A'AGCT_T
Cuts at: 748 2191 4293 748
Size: 1443 2102 3476
Fragments arranged by size:
3476 2102 1443
HpaI GTT'AAC
Cuts at: 4611 4611
Size: 7021
KpnI G_GTAC'C
Cuts at: 4346 4346
Size: 7021
MfeI C'AATT_G
Cuts at: 4620 4620
Size: 7021
MluI A'CGCG_T
Cuts at: 6897 6897
Size: 7021
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2167 6135 6319 1305
Size: 862 3968 184 2007
Fragments arranged by size:
3968 2007 862 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2529 4013 4280 10
Size: 54 1425 343 697 1484 267 2751
Fragments arranged by size:
2751 1484 1425 697 343 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2181 2448 2649 2694 4979 5338 5497
Size: 648 1015 267 201 45 2285 359 159
Cuts at: 5497 518
Size: 2042
Fragments arranged by size:
2285 2042 1015 648 359 267 201 159
45
MspA1I CmG'CkG
Cuts at: 1606 3769 3922 4070 4437 5114 6262 6507 1606
Size: 2163 153 148 367 677 1148 245 2120
Fragments arranged by size:
2163 2120 1148 677 367 245 153 148
NaeI GCC'GGC
Cuts at: 3399 4170 3399
Size: 771 6250
NcoI C'CATG_G
Cuts at: 513 1065 2004 2704 4110 513
Size: 552 939 700 1406 3424
Fragments arranged by size:
3424 1406 939 700 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7021
NgoMIV G'CCGG_C
Cuts at: 3397 4168 3397
Size: 771 6250
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7021
NotI GC'GGCC_GC
Cuts at: 4468 4468
Size: 7021
NsiI A_TGCA'T
Cuts at: 3671 3671
Size: 7021
NspI r_CATG'y
Cuts at: 1132 1229 2186 3962 4379 5924 1132
Size: 97 957 1776 417 1545 2229
Fragments arranged by size:
2229 1776 1545 957 417 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7021
PciI A'CATG_T
Cuts at: 5920 5920
Size: 7021
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2032 1108
Size: 924 6097
PmeI GTTT'AAAC
Cuts at: 4325 4325
Size: 7021
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4295 4327 5090 5122 6603 6635 4295
Size: 32 763 32 1481 32 4681
Fragments arranged by size:
4681 1481 763 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2203 2203
Size: 7021
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7021
PsiI TTA'TAA
Cuts at: 4591 4591
Size: 7021
PspXI vC'TCGA_Gb
Cuts at: 3506 3506
Size: 7021
PstI C_TGCA'G
Cuts at: 830 2046 2313 4373 830
Size: 1216 267 2060 3478
Fragments arranged by size:
3478 2060 1216 267
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7021
PvuII CAG'CTG
Cuts at: 3769 4070 3769
Size: 301 6720
SacI G_AGCT'C
Cuts at: 2421 2421
Size: 7021
SalI G'TCGA_C
Cuts at: 1936 2576 4363 1936
Size: 640 1787 4594
Fragments arranged by size:
4594 1787 640
SapI GCTCTTCn'nnn_
Cuts at: 2270 2270
Size: 7021
SbfI CC_TGCA'GG
Cuts at: 4373 4373
Size: 7021
ScaI AGT'ACT
Cuts at: 3678 3933 5269 6981 3678
Size: 255 1336 1712 3718
Fragments arranged by size:
3718 1712 1336 255
SfcI C'TryA_G
Cuts at: 826 1045 2042 2309 2374 2584 4369 5504 6185
Size: 219 997 267 65 210 1785 1135 681
Cuts at: 6185 6376 826
Size: 191 1471
Fragments arranged by size:
1785 1471 1135 997 681 267 219 210
191 65
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7021
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7021
SmaI CCC'GGG
Cuts at: 1872 4348 1872
Size: 2476 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2420 3506 5125 6026 6288 820
Size: 197 926 477 1086 1619 901 262 1553
Fragments arranged by size:
1619 1553 1086 926 901 477 262 197
SnaBI TAC'GTA
Cuts at: 493 4812 493
Size: 4319 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7021
SphI G_CATG'C
Cuts at: 1132 4379 1132
Size: 3247 3774
SspI AAT'ATT
Cuts at: 5 52 2090 4945 5
Size: 47 2038 2855 2081
Fragments arranged by size:
2855 2081 2038 47
StuI AGG'CCT
Cuts at: 1517 2792 1517
Size: 1275 5746
StyI C'CwwG_G
Cuts at: 513 1065 2004 2704 3081 3369 4110 4241 4460
Size: 552 939 700 377 288 741 131 219
Cuts at: 4460 513
Size: 3074
Fragments arranged by size:
3074 939 741 700 552 377 288 219
131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2684 5048 5065 5218 5403 5848 325
Size: 1255 1104 2364 17 153 185 445 1498
Fragments arranged by size:
2364 1498 1255 1104 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3676 3755 3931
Size: 274 80 33 51 1443 1699 79 176
Cuts at: 3931 5267 6979 96
Size: 1336 1712 138
Fragments arranged by size:
1712 1699 1443 1336 274 176 138 80
79 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2573 2605 2729 2761 6981 7013 2573
Size: 32 124 32 4220 32 2581
Fragments arranged by size:
4220 2581 124 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7021
XbaI T'CTAG_A
Cuts at: 4357 4357
Size: 7021
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2206 2206
Size: 7021
XhoI C'TCGA_G
Cuts at: 1943 2420 3506 1943
Size: 477 1086 5458
Fragments arranged by size:
5458 1086 477
XmaI C'CCGG_G
Cuts at: 1870 4346 1870
Size: 2476 4545
XmnI GAAnn'nnTTC
Cuts at: 3595 4307 5150 3595
Size: 712 843 5466
Fragments arranged by size:
5466 843 712
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4030 4829 276
Size: 53 83 186 936 2496 799 2468
Fragments arranged by size:
2496 2468 936 799 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI BaeI BbeI BsiWI BsmBI BstZ17I FseI
FspAI KasI NarI NruI PacI PfoI PmlI PspOMI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI