Restriction map for
pFN21AB6266
(Circular) (Six-base) MAPSORT of: pFN21AB6266.seq Check: 1967 from: 1 to: 7015
pFN21AB6266 7015 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4098 4098
Size: 7015
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3719 4825 278
Size: 53 83 186 936 2183 1106 2468
Fragments arranged by size:
2468 2183 1106 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4358 1937
Size: 2421 4594
Acc65I G'GTAC_C
Cuts at: 4336 4336
Size: 7015
AclI AA'CG_TT
Cuts at: 1563 5142 5515 1563
Size: 3579 373 3063
Fragments arranged by size:
3579 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2438 5078 6462 6730 1632
Size: 806 2640 1384 268 1917
Fragments arranged by size:
2640 1917 1384 806 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6818
AflIII A'CryG_T
Cuts at: 1203 3192 3496 3498 5914 6891 1203
Size: 1989 304 2 2416 977 1327
Fragments arranged by size:
2416 1989 1327 977 304 2
AgeI A'CCGG_T
Cuts at: 5846 5846
Size: 7015
AhdI GACnn_n'nnGTC
Cuts at: 5744 5744
Size: 7015
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7015
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2058 2580 1184
Size: 874 522 5619
Fragments arranged by size:
5619 874 522
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2090 2122 2409 2441 2090
Size: 32 287 32 6664
Fragments arranged by size:
6664 287 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2110 3170 3930 4758 6330 2110
Size: 1060 760 828 1572 2795
Fragments arranged by size:
2795 1572 1060 828 760
ApaLI G'TGCA_C
Cuts at: 2271 5072 6228 2271
Size: 2801 1156 3058
Fragments arranged by size:
3058 2801 1156
ApoI r'AATT_y
Cuts at: 1493 2099 2660 4118 4324 4550 4702 6811 1493
Size: 606 561 1458 206 226 152 2109 1697
Fragments arranged by size:
2109 1697 1458 606 561 226 206 152
AseI AT'TA_AT
Cuts at: 160 1032 5569 160
Size: 872 4537 1606
Fragments arranged by size:
4537 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7015
AvaI C'yCGr_G
Cuts at: 1870 1943 2477 2808 4276 4340 1870
Size: 73 534 331 1468 64 4545
Fragments arranged by size:
4545 1468 534 331 73 64
BamHI G'GATC_C
Cuts at: 4345 4712 4345
Size: 367 6648
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2022 2993 3887 4336 5791
Size: 325 217 627 235 971 894 449 1455
Cuts at: 5791 618
Size: 1842
Fragments arranged by size:
1842 1455 971 894 627 449 325 235
217
BanII G_rGCy'C
Cuts at: 1391 3563 4334 1391
Size: 2172 771 4072
Fragments arranged by size:
4072 2172 771
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6044
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5206 5240 1903
Size: 34 3269 34 3678
Fragments arranged by size:
3678 3269 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4907 6117 4907
Size: 1210 5805
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7015
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2882 3224 3236
Size: 107 122 71 631 605 1210 342 12
Cuts at: 3236 3549 5626 136
Size: 313 2077 1525
Fragments arranged by size:
2077 1525 1210 631 605 342 313 122
107 71 12
BglII A'GATC_T
Cuts at: 3981 7010 3981
Size: 3029 3986
BlpI GC'TnA_GC
Cuts at: 2394 4232 4432 2394
Size: 1838 200 4977
Fragments arranged by size:
4977 1838 200
Bme1580I G_kGCm'C
Cuts at: 1790 2275 2597 2996 3099 5076 6232 1790
Size: 485 322 399 103 1977 1156 2573
Fragments arranged by size:
2573 1977 1156 485 399 322 103
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7015
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2417 4746 5704
Size: 416 53 83 399 24 1000 2329 958
Cuts at: 5704 442
Size: 1753
Fragments arranged by size:
2329 1753 1000 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2689 2991 3021 5675 1239
Size: 1450 302 30 2654 2579
Fragments arranged by size:
2654 2579 1450 302 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3097 5140 6005 6303 3097
Size: 2043 865 298 3809
Fragments arranged by size:
3809 2043 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3029 5678 882
Size: 617 1530 2649 2219
Fragments arranged by size:
2649 2219 1530 617
BsaAI yAC'GTr
Cuts at: 493 1204 3499 4060 4806 493
Size: 711 2295 561 746 2702
Fragments arranged by size:
2702 2295 746 711 561
BsaBI GATnn'nnATC
Cuts at: 7009 7009
Size: 7015
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2030 3396 3716 4822
Size: 53 83 186 936 497 1366 320 1106
Cuts at: 4822 5204 5839 275
Size: 382 635 1451
Fragments arranged by size:
1451 1366 1106 936 635 497 382 320
186 83 53
BsaWI w'CCGG_w
Cuts at: 2085 5448 5846 6120 6267 6678 2085
Size: 3363 398 274 147 411 2422
Fragments arranged by size:
3363 2422 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3233 3263 3286 3316 3449
Size: 30 165 30 1830 30 23 30 133
Cuts at: 3449 3479 3701 3731 3959 3989 1178
Size: 30 222 30 228 30 4204
Fragments arranged by size:
4204 1830 228 222 165 133 30 30
30 30 30 30 30 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2869 4157 1186
Size: 1683 1288 4044
Fragments arranged by size:
4044 1683 1288
BseYI C'CCAG_C
Cuts at: 3570 6218 6714 3570
Size: 2648 496 3871
Fragments arranged by size:
3871 2648 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3616 3797 4040 4070 1163
Size: 573 1880 181 243 30 4108
Fragments arranged by size:
4108 1880 573 243 181 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3228 4465 5226 6254 1540
Size: 275 186 1227 1237 761 1028 2301
Fragments arranged by size:
2301 1237 1227 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2275 2515 2574 5076 5161 6232 2275
Size: 240 59 2502 85 1071 3058
Fragments arranged by size:
3058 2502 1071 240 85 59
BsmI GAATG_Cn'
Cuts at: 2368 4526 4619 2368
Size: 2158 93 4764
Fragments arranged by size:
4764 2158 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2275 2515 2574 2597 2996 3099 3563
Size: 399 485 240 59 23 399 103 464
Cuts at: 3563 4334 5076 5161 6232 1391
Size: 771 742 85 1071 2174
Fragments arranged by size:
2174 1071 771 742 485 464 399 399
240 103 85 59 23
BspEI T'CCGG_A
Cuts at: 6678 6678
Size: 7015
BspHI T'CATG_A
Cuts at: 4904 6634 4904
Size: 1730 5285
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3343 3945 4098 4370 844
Size: 2499 602 153 272 3489
Fragments arranged by size:
3489 2499 602 272 153
BsrBI CCG'CTC
Cuts at: 1394 1823 4902 1394
Size: 429 3079 3507
Fragments arranged by size:
3507 3079 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4048 5510 5684 65
Size: 1183 2800 1462 174 1396
Fragments arranged by size:
2800 1462 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2019 2489 5659 5846 1700
Size: 110 209 470 3170 187 2869
Fragments arranged by size:
3170 2869 470 209 187 110
BsrGI T'GTAC_A
Cuts at: 96 3501 96
Size: 3405 3610
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7015
BssSI C'ACGA_G
Cuts at: 3490 5075 6087 3490
Size: 1585 1012 4418
Fragments arranged by size:
4418 1585 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2386 2795 794
Size: 1592 409 5014
Fragments arranged by size:
5014 1592 409
BstBI TT'CG_AA
Cuts at: 5819 5819
Size: 7015
BstEII G'GTnAC_C
Cuts at: 1184 2344 1184
Size: 1160 5855
BstYI r'GATC_y
Cuts at: 1972 3808 3981 4345 4712 5097 5114 6566 6652
Size: 1836 173 364 367 385 17 1452 86
Cuts at: 6652 6664 7010 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1836 1452 385 367 364 346 173
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2349 2413 2871 3222 3231 3255 3451
Size: 552 1284 64 458 351 9 24 196
Cuts at: 3451 513
Size: 4077
Fragments arranged by size:
4077 1284 552 458 351 196 64 24
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2751 3781 487
Size: 683 56 1525 1030 3721
Fragments arranged by size:
3721 1525 1030 683 56
BtsI GCAGTG_nn'
Cuts at: 2491 3099 4029 4531 5325 5345 2491
Size: 608 930 502 794 20 4161
Fragments arranged by size:
4161 930 794 608 502 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7015
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6980
DraI TTT'AAA
Cuts at: 4319 4319
Size: 7015
DraIII CAC_nnn'GTG
Cuts at: 2188 2188
Size: 7015
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3920 6022 809
Size: 507 2604 2102 1802
Fragments arranged by size:
2604 2102 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2410 3225 3744
Size: 54 1425 50 275 18 580 815 519
Cuts at: 3744 4462 5351 6980 8
Size: 718 889 1629 43
Fragments arranged by size:
1629 1425 889 815 718 580 519 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3225 4462 1537
Size: 275 1413 1237 4090
Fragments arranged by size:
4090 1413 1237 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4945 1353
Size: 3592 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3722 4195 5602 5976 6122 387
Size: 1064 138 2133 473 1407 374 146 1280
Fragments arranged by size:
2133 1407 1280 1064 473 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2438 2689 2991 3021 5078 5675 6462
Size: 393 806 251 302 30 2057 597 787
Cuts at: 6462 6730 1239
Size: 268 1524
Fragments arranged by size:
2057 1524 806 787 597 393 302 268
251 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3941 3941
Size: 7015
EcoO109I rG'GnC_Cy
Cuts at: 2770 3676 3927 3934 2770
Size: 906 251 7 5851
Fragments arranged by size:
5851 906 251 7
EcoRI G'AATT_C
Cuts at: 2099 4324 4702 2099
Size: 2225 378 4412
Fragments arranged by size:
4412 2225 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2910 3787 2910
Size: 877 6138
FspI TGC'GCA
Cuts at: 5521 5521
Size: 7015
HaeII r_GCGC'y
Cuts at: 1678 3003 3171 3213 3250 6162 1678
Size: 1325 168 42 37 2912 2531
Fragments arranged by size:
2912 2531 1325 168 42 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2687 2719 3399 3431 3701
Size: 32 135 32 1115 32 680 32 270
Cuts at: 3701 3733 3959 3991 4208 4240 5656 5688 5730
Size: 32 226 32 217 32 1416 32 42
Cuts at: 5730 5762 1373
Size: 32 2626
Fragments arranged by size:
2626 1416 1115 680 270 226 217 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3058 4359 4605 669
Size: 988 281 1120 1301 246 3079
Fragments arranged by size:
3079 1301 1120 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7015
HpaI GTT'AAC
Cuts at: 3058 4605 3058
Size: 1547 5468
KpnI G_GTAC'C
Cuts at: 4340 4340
Size: 7015
MfeI C'AATT_G
Cuts at: 4614 4614
Size: 7015
MluI A'CGCG_T
Cuts at: 6891 6891
Size: 7015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3713 6129 6313 1305
Size: 2408 2416 184 2007
Fragments arranged by size:
2416 2408 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2412 3746 10
Size: 54 1425 343 580 1334 3279
Fragments arranged by size:
3279 1425 1334 580 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 2562 3800 4092 4247 4973 5332
Size: 648 1059 337 1238 292 155 726 359
Cuts at: 5332 5491 518
Size: 159 2042
Fragments arranged by size:
2042 1238 1059 726 648 359 337 292
159 155
MspA1I CmG'CkG
Cuts at: 1606 3071 3224 3864 3955 4084 4431 5108 6256
Size: 1465 153 640 91 129 347 677 1148
Cuts at: 6256 6501 1606
Size: 245 2120
Fragments arranged by size:
2120 1465 1148 677 640 347 245 153
129 91
NaeI GCC'GGC
Cuts at: 2021 2491 2021
Size: 470 6545
NcoI C'CATG_G
Cuts at: 513 1065 2349 3255 513
Size: 552 1284 906 4273
Fragments arranged by size:
4273 1284 906 552
NdeI CA'TA_TG
Cuts at: 387 2209 387
Size: 1822 5193
NgoMIV G'CCGG_C
Cuts at: 2019 2489 2019
Size: 470 6545
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7015
NotI GC'GGCC_GC
Cuts at: 3225 4462 3225
Size: 1237 5778
NspI r_CATG'y
Cuts at: 1132 1229 2983 3196 3428 4373 5918 1132
Size: 97 1754 213 232 945 1545 2229
Fragments arranged by size:
2229 1754 1545 945 232 213 97
PasI CC'CwG_GG
Cuts at: 1348 3323 3991 1348
Size: 1975 668 4372
Fragments arranged by size:
4372 1975 668
PciI A'CATG_T
Cuts at: 3192 5914 3192
Size: 2722 4293
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3897 1108
Size: 2789 4226
PfoI T'CCnGG_A
Cuts at: 2070 2461 2070
Size: 391 6624
PmeI GTTT'AAAC
Cuts at: 4319 4319
Size: 7015
PmlI CAC'GTG
Cuts at: 3499 4060 3499
Size: 561 6454
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5084 5116 6597 6629 5084
Size: 32 1481 32 5470
Fragments arranged by size:
5470 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2770 3676 3927 3934 2770
Size: 906 251 7 5851
Fragments arranged by size:
5851 906 251 7
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7015
PsiI TTA'TAA
Cuts at: 4585 4585
Size: 7015
PstI C_TGCA'G
Cuts at: 830 2047 2115 3361 3785 3960 4367 830
Size: 1217 68 1246 424 175 407 3478
Fragments arranged by size:
3478 1246 1217 424 407 175 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7015
PvuII CAG'CTG
Cuts at: 3071 3864 3955 4084 3071
Size: 793 91 129 6002
Fragments arranged by size:
6002 793 129 91
SacII CC_GC'GG
Cuts at: 3225 3225
Size: 7015
SalI G'TCGA_C
Cuts at: 1936 4357 1936
Size: 2421 4594
SbfI CC_TGCA'GG
Cuts at: 2115 4367 2115
Size: 2252 4763
ScaI AGT'ACT
Cuts at: 3661 4150 5263 6975 3661
Size: 489 1113 1712 3701
Fragments arranged by size:
3701 1712 1113 489
SfcI C'TryA_G
Cuts at: 826 1045 2043 2111 3357 3781 3956 4293 4363
Size: 219 998 68 1246 424 175 337 70
Cuts at: 4363 5498 6179 6370 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1246 1135 998 681 424 337 219
191 175 70 68
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7015
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7015
SmaI CCC'GGG
Cuts at: 1872 2810 4342 1872
Size: 938 1532 4545
Fragments arranged by size:
4545 1532 938
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3076 5119 6020 6282 820
Size: 197 926 1133 2043 901 262 1553
Fragments arranged by size:
2043 1553 1133 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4806 493
Size: 4313 2702
SpeI A'CTAG_T
Cuts at: 152 3826 152
Size: 3674 3341
SphI G_CATG'C
Cuts at: 1132 3428 4373 1132
Size: 2296 945 3774
Fragments arranged by size:
3774 2296 945
SspI AAT'ATT
Cuts at: 5 52 4939 5
Size: 47 4887 2081
Fragments arranged by size:
4887 2081 47
StuI AGG'CCT
Cuts at: 1517 3012 1517
Size: 1495 5520
StyI C'CwwG_G
Cuts at: 513 1065 2349 3255 3762 4454 513
Size: 552 1284 906 507 692 3074
Fragments arranged by size:
3074 1284 906 692 552 507
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5042 5059 5212 5397 5842 325
Size: 1255 3462 17 153 185 445 1498
Fragments arranged by size:
3462 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3501 3659 4148
Size: 274 80 33 51 1443 1524 158 489
Cuts at: 4148 4260 5261 6973 96
Size: 112 1001 1712 138
Fragments arranged by size:
1712 1524 1443 1001 489 274 158 138
112 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2265 2297 3449 3481 6975 7007 2265
Size: 32 1152 32 3494 32 2273
Fragments arranged by size:
3494 2273 1152 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7015
XbaI T'CTAG_A
Cuts at: 4351 4351
Size: 7015
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3068 3676 3068
Size: 608 6407
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7015
XmaI C'CCGG_G
Cuts at: 1870 2808 4340 1870
Size: 938 1532 4545
Fragments arranged by size:
4545 1532 938
XmnI GAAnn'nnTTC
Cuts at: 5144 5144
Size: 7015
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3717 4823 276
Size: 53 83 186 936 2183 1106 2468
Fragments arranged by size:
2468 2183 1106 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SfoI SrfI SwaI