Restriction map for
pFN21AB6265
(Circular) (Six-base) MAPSORT of: pFN21AB6265.seq Check: 4044 from: 1 to: 6775
pFN21AB6265 6775 bp
With 182 enzymes: SgfI *
March 28, 2012 13:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4585 278
Size: 53 83 186 936 3049 2468
Fragments arranged by size:
3049 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4118 1937
Size: 2181 4594
Acc65I G'GTAC_C
Cuts at: 4096 4096
Size: 6775
AclI AA'CG_TT
Cuts at: 1563 4902 5275 1563
Size: 3339 373 3063
Fragments arranged by size:
3339 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2122 2589 3618 3669 3813 3875 4838 6222
Size: 490 467 1029 51 144 62 963 1384
Cuts at: 6222 6490 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1029 963 490 467 268 144
62 51
AflII C'TTAA_G
Cuts at: 820 1017 3353 820
Size: 197 2336 4242
Fragments arranged by size:
4242 2336 197
AflIII A'CryG_T
Cuts at: 1203 5674 6651 1203
Size: 4471 977 1327
Fragments arranged by size:
4471 1327 977
AgeI A'CCGG_T
Cuts at: 5606 5606
Size: 6775
AhdI GACnn_n'nnGTC
Cuts at: 5504 5504
Size: 6775
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6775
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6775
AlwNI CAG_nnn'CTG
Cuts at: 3955 4518 6090 3955
Size: 563 1572 4640
Fragments arranged by size:
4640 1572 563
ApaLI G'TGCA_C
Cuts at: 2115 4832 5988 2115
Size: 2717 1156 2902
Fragments arranged by size:
2902 2717 1156
ApoI r'AATT_y
Cuts at: 1493 2832 3055 3842 4084 4310 4462 6571 1493
Size: 1339 223 787 242 226 152 2109 1697
Fragments arranged by size:
2109 1697 1339 787 242 226 223 152
AseI AT'TA_AT
Cuts at: 160 1032 5329 160
Size: 872 4297 1606
Fragments arranged by size:
4297 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6775
AvaI C'yCGr_G
Cuts at: 1870 1943 3860 4100 1870
Size: 73 1917 240 4545
Fragments arranged by size:
4545 1917 240 73
AvrII C'CTAG_G
Cuts at: 2509 2509
Size: 6775
BamHI G'GATC_C
Cuts at: 4105 4472 4105
Size: 367 6408
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4096 5551 618
Size: 325 217 627 2309 1455 1842
Fragments arranged by size:
2309 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3392 3672 3703 3861 4094 1391
Size: 2001 280 31 158 233 4072
Fragments arranged by size:
4072 2001 280 233 158 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2283 928
Size: 971 384 5420
Fragments arranged by size:
5420 971 384
BbvCI CC'TCA_GC
Cuts at: 2913 2913
Size: 6775
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4966 5000 1903
Size: 34 3029 34 3678
Fragments arranged by size:
3678 3029 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3643 4667 5877 3643
Size: 1024 1210 4541
Fragments arranged by size:
4541 1210 1024
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6775
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5386 136
Size: 107 122 71 631 605 3714 1525
Fragments arranged by size:
3714 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6770 6770
Size: 6775
BlpI GC'TnA_GC
Cuts at: 2866 4192 2866
Size: 1326 5449
Bme1580I G_kGCm'C
Cuts at: 1790 2119 4836 5992 1790
Size: 329 2717 1156 2573
Fragments arranged by size:
2717 2573 1156 329
BmgBI CAC'GTC
Cuts at: 1318 2154 1318
Size: 836 5939
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4506 5464 442
Size: 416 53 83 399 24 3089 958 1753
Fragments arranged by size:
3089 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6775
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2220 2925 4031 5435 1239
Size: 981 705 1106 1404 2579
Fragments arranged by size:
2579 1404 1106 981 705
Bpu10I CC'TnA_GC
Cuts at: 2913 2913
Size: 6775
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2263 3645 4900 5765 6063 2263
Size: 1382 1255 865 298 2975
Fragments arranged by size:
2975 1382 1255 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2751 5438 882
Size: 617 1252 2687 2219
Fragments arranged by size:
2687 2219 1252 617
BsaAI yAC'GTr
Cuts at: 493 1204 4566 493
Size: 711 3362 2702
Fragments arranged by size:
3362 2702 711
BsaBI GATnn'nnATC
Cuts at: 3996 6769 3996
Size: 2773 4002
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4582 4964 5599 275
Size: 53 83 186 936 3049 382 635 1451
Fragments arranged by size:
3049 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5208 5606 5880 6027 6438 5208
Size: 398 274 147 411 5545
Fragments arranged by size:
5545 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6550
Fragments arranged by size:
6550 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3189 3534 1186
Size: 2003 345 4427
Fragments arranged by size:
4427 2003 345
BseYI C'CCAG_C
Cuts at: 2716 3590 5978 6474 2716
Size: 874 2388 496 3017
Fragments arranged by size:
3017 2388 874 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3552 1163
Size: 573 1816 4386
Fragments arranged by size:
4386 1816 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4225 4986 6014 1540
Size: 275 186 2224 761 1028 2301
Fragments arranged by size:
2301 2224 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2119 2959 3310 3861 4836 4921 5992 2119
Size: 840 351 551 975 85 1071 2902
Fragments arranged by size:
2902 1071 975 840 551 351 85
BsmI GAATG_Cn'
Cuts at: 4286 4379 4286
Size: 93 6682
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2119 2959 3310 3392 3672 3703 3861
Size: 399 329 840 351 82 280 31 158
Cuts at: 3861 4094 4836 4921 5992 1391
Size: 233 742 85 1071 2174
Fragments arranged by size:
2174 1071 840 742 399 351 329 280
233 158 85 82 31
BspEI T'CCGG_A
Cuts at: 6438 6438
Size: 6775
BspHI T'CATG_A
Cuts at: 4664 6394 4664
Size: 1730 5045
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4130 844
Size: 3286 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4662 1394
Size: 429 2839 3507
Fragments arranged by size:
3507 2839 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2889 2901 3927 5270 5444 65
Size: 1183 1641 12 1026 1343 174 1396
Fragments arranged by size:
1641 1396 1343 1183 1026 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 5419 5606 1700
Size: 110 3609 187 2869
Fragments arranged by size:
3609 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3162 96
Size: 3066 3709
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6775
BssSI C'ACGA_G
Cuts at: 4835 5847 4835
Size: 1012 5763
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3955 794
Size: 3161 3614
BstBI TT'CG_AA
Cuts at: 5579 5579
Size: 6775
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6775
BstXI CCAn_nnnn'nTGG
Cuts at: 2896 2896
Size: 6775
BstYI r'GATC_y
Cuts at: 1972 4105 4472 4857 4874 6326 6412 6424 6770
Size: 2133 367 385 17 1452 86 12 346
Cuts at: 6770 1972
Size: 1977
Fragments arranged by size:
2133 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3128 513
Size: 552 2063 4160
Fragments arranged by size:
4160 2063 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6036
Fragments arranged by size:
6036 683 56
BtsI GCAGTG_nn'
Cuts at: 3953 4291 5085 5105 3953
Size: 338 794 20 5623
Fragments arranged by size:
5623 794 338 20
ClaI AT'CG_AT
Cuts at: 1554 2948 1554
Size: 1394 5381
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2642 2677 529
Size: 35 2078 35 4627
Fragments arranged by size:
4627 2078 35 35
DraI TTT'AAA
Cuts at: 3886 4079 3886
Size: 193 6582
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2246 5782 809
Size: 507 930 3536 1802
Fragments arranged by size:
3536 1802 930 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4222 5111 6740
Size: 54 1425 50 275 18 2392 889 1629
Cuts at: 6740 8
Size: 43
Fragments arranged by size:
2392 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4222 1537
Size: 275 2410 4090
Fragments arranged by size:
4090 2410 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2608 2931 3660 3705 4705 1353
Size: 1255 323 729 45 1000 3423
Fragments arranged by size:
3423 1255 1000 729 323 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5362 5736 5882 387
Size: 1064 138 3773 374 146 1280
Fragments arranged by size:
3773 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3859 3859
Size: 6775
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2122 2220 2589 2925 3618 3669 3813
Size: 393 490 98 369 336 693 51 144
Cuts at: 3813 3875 4031 4838 5435 6222 6490 1239
Size: 62 156 807 597 787 268 1524
Fragments arranged by size:
1524 807 787 693 597 490 393 369
336 268 156 144 98 62 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3516 3600 3516
Size: 84 6691
EcoO109I rG'GnC_Cy
Cuts at: 3524 3524
Size: 6775
EcoRI G'AATT_C
Cuts at: 2832 4084 4462 2832
Size: 1252 378 5145
Fragments arranged by size:
5145 1252 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 6775
FspI TGC'GCA
Cuts at: 5281 5281
Size: 6775
HaeII r_GCGC'y
Cuts at: 1678 5922 1678
Size: 4244 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2617 2649 2930 2962 3454
Size: 32 135 32 1045 32 281 32 492
Cuts at: 3454 3486 3617 3649 4010 4042 5416 5448 5490
Size: 32 131 32 361 32 1374 32 42
Cuts at: 5490 5522 1373
Size: 32 2626
Fragments arranged by size:
2626 1374 1045 492 361 281 135 131
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3439 4119 4365 669
Size: 988 281 1501 680 246 3079
Fragments arranged by size:
3079 1501 988 680 281 246
HindIII A'AGCT_T
Cuts at: 748 2613 3907 748
Size: 1865 1294 3616
Fragments arranged by size:
3616 1865 1294
HpaI GTT'AAC
Cuts at: 4365 4365
Size: 6775
KpnI G_GTAC'C
Cuts at: 4100 4100
Size: 6775
MfeI C'AATT_G
Cuts at: 3221 4374 3221
Size: 1153 5622
MluI A'CGCG_T
Cuts at: 6651 6651
Size: 6775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5889 6073 1305
Size: 4584 184 2007
Fragments arranged by size:
4584 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4953
Fragments arranged by size:
4953 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2894 4733 5092 5251 518
Size: 648 1728 1839 359 159 2042
Fragments arranged by size:
2042 1839 1728 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4191 4868 6016 6261 1606
Size: 2585 677 1148 245 2120
Fragments arranged by size:
2585 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6223
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6775
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6775
NotI GC'GGCC_GC
Cuts at: 4222 4222
Size: 6775
NspI r_CATG'y
Cuts at: 1132 1229 4133 5678 1132
Size: 97 2904 1545 2229
Fragments arranged by size:
2904 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6775
PciI A'CATG_T
Cuts at: 5674 5674
Size: 6775
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6775
PmeI GTTT'AAAC
Cuts at: 4079 4079
Size: 6775
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4844 4876 6357 6389 4844
Size: 32 1481 32 5230
Fragments arranged by size:
5230 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6775
PsiI TTA'TAA
Cuts at: 3825 4345 3825
Size: 520 6255
PstI C_TGCA'G
Cuts at: 830 4127 830
Size: 3297 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6775
SacI G_AGCT'C
Cuts at: 3861 3861
Size: 6775
SalI G'TCGA_C
Cuts at: 1936 4117 1936
Size: 2181 4594
SapI GCTCTTCn'nnn_
Cuts at: 2931 3660 2931
Size: 729 6046
SbfI CC_TGCA'GG
Cuts at: 4127 4127
Size: 6775
ScaI AGT'ACT
Cuts at: 5023 6735 5023
Size: 1712 5063
SfcI C'TryA_G
Cuts at: 826 1045 2028 2767 3311 4123 5258 5939 6130
Size: 219 983 739 544 812 1135 681 191
Cuts at: 6130 826
Size: 1471
Fragments arranged by size:
1471 1135 983 812 739 681 544 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6775
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6775
SmaI CCC'GGG
Cuts at: 1872 4102 1872
Size: 2230 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2278 3353 3660 3860 4879 5780
Size: 197 926 335 1075 307 200 1019 901
Cuts at: 5780 6042 820
Size: 262 1553
Fragments arranged by size:
1553 1075 1019 926 901 335 307 262
200 197
SnaBI TAC'GTA
Cuts at: 493 4566 493
Size: 4073 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6775
SphI G_CATG'C
Cuts at: 1132 4133 1132
Size: 3001 3774
SspI AAT'ATT
Cuts at: 5 52 2266 3073 4699 5
Size: 47 2214 807 1626 2081
Fragments arranged by size:
2214 2081 1626 807 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6775
StyI C'CwwG_G
Cuts at: 513 1065 2509 3278 4214 513
Size: 552 1444 769 936 3074
Fragments arranged by size:
3074 1444 936 769 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3351 4802 4819 4972 5157 5602 325
Size: 1255 1771 1451 17 153 185 445 1498
Fragments arranged by size:
1771 1498 1451 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2608 3020 3094
Size: 274 80 33 51 1443 631 412 74
Cuts at: 3094 3155 3162 5021 6733 96
Size: 61 7 1859 1712 138
Fragments arranged by size:
1859 1712 1443 631 412 274 138 80
74 61 51 33 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2394 2426 6735 6767 2394
Size: 32 4309 32 2402
Fragments arranged by size:
4309 2402 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6775
XbaI T'CTAG_A
Cuts at: 3296 4111 3296
Size: 815 5960
XhoI C'TCGA_G
Cuts at: 1943 3860 1943
Size: 1917 4858
XmaI C'CCGG_G
Cuts at: 1870 4100 1870
Size: 2230 4545
XmnI GAAnn'nnTTC
Cuts at: 2602 3450 3681 4904 2602
Size: 848 231 1223 4473
Fragments arranged by size:
4473 1223 848 231
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4583 276
Size: 53 83 186 936 3049 2468
Fragments arranged by size:
3049 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI