Restriction map for
pFN21AB6260
(Circular) (Six-base) MAPSORT of: pFN21AB6260.seq Check: 111 from: 1 to: 6886
pFN21AB6260 6886 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3672 3672
Size: 6886
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4696 278
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4229 1937
Size: 2292 4594
Acc65I G'GTAC_C
Cuts at: 4207 4207
Size: 6886
AclI AA'CG_TT
Cuts at: 1563 4185 5013 5386 1563
Size: 2622 828 373 3063
Fragments arranged by size:
3063 2622 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3692 4023 4949 6333 6601 1632
Size: 2060 331 926 1384 268 1917
Fragments arranged by size:
2060 1917 1384 926 331 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6689
AflIII A'CryG_T
Cuts at: 1203 2202 2287 5785 6762 1203
Size: 999 85 3498 977 1327
Fragments arranged by size:
3498 1327 999 977 85
AgeI A'CCGG_T
Cuts at: 5717 5717
Size: 6886
AhdI GACnn_n'nnGTC
Cuts at: 2068 5615 2068
Size: 3547 3339
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6886
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6886
AlwNI CAG_nnn'CTG
Cuts at: 2617 3637 3702 4175 4629 6201 2617
Size: 1020 65 473 454 1572 3302
Fragments arranged by size:
3302 1572 1020 473 454 65
ApaLI G'TGCA_C
Cuts at: 3776 4943 6099 3776
Size: 1167 1156 4563
Fragments arranged by size:
4563 1167 1156
ApoI r'AATT_y
Cuts at: 1493 3559 3578 4195 4421 4573 6682 1493
Size: 2066 19 617 226 152 2109 1697
Fragments arranged by size:
2109 2066 1697 617 226 152 19
AseI AT'TA_AT
Cuts at: 160 1032 5440 160
Size: 872 4408 1606
Fragments arranged by size:
4408 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
AvaI C'yCGr_G
Cuts at: 1870 1943 2978 3267 3338 4211 1870
Size: 73 1035 289 71 873 4545
Fragments arranged by size:
4545 1035 873 289 73 71
BamHI G'GATC_C
Cuts at: 4216 4583 4216
Size: 367 6519
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3541 4207 5662 618
Size: 325 217 627 1754 666 1455 1842
Fragments arranged by size:
1842 1754 1455 666 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2829 3189 4205 1391
Size: 1438 360 1016 4072
Fragments arranged by size:
4072 1438 1016 360
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2085 3507 4092 928
Size: 971 186 1422 585 3722
Fragments arranged by size:
3722 1422 971 585 186
BbvCI CC'TCA_GC
Cuts at: 3357 3357
Size: 6886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5077 5111 1903
Size: 34 3140 34 3678
Fragments arranged by size:
3678 3140 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4778 5988 4778
Size: 1210 5676
BclI T'GATC_A
Cuts at: 1548 2935 1548
Size: 1387 5499
BfrBI ATG'CAT
Cuts at: 2103 2103
Size: 6886
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3118 3272 5497
Size: 107 122 71 631 605 1446 154 2225
Cuts at: 5497 136
Size: 1525
Fragments arranged by size:
2225 1525 1446 631 605 154 122 107
71
BglII A'GATC_T
Cuts at: 3363 6881 3363
Size: 3518 3368
BlpI GC'TnA_GC
Cuts at: 4303 4303
Size: 6886
Bme1580I G_kGCm'C
Cuts at: 1790 2853 3544 3780 4947 6103 1790
Size: 1063 691 236 1167 1156 2573
Fragments arranged by size:
2573 1167 1156 1063 691 236
BmgBI CAC'GTC
Cuts at: 1318 2848 1318
Size: 1530 5356
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2176 4617 5575
Size: 416 53 83 399 24 759 2441 958
Cuts at: 5575 442
Size: 1753
Fragments arranged by size:
2441 1753 958 759 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6886
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3822 3856 3822
Size: 34 6852
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2217 3531 4126 5546 1239
Size: 978 1314 595 1420 2579
Fragments arranged by size:
2579 1420 1314 978 595
Bpu10I CC'TnA_GC
Cuts at: 2280 3183 3357 2280
Size: 903 174 5809
Fragments arranged by size:
5809 903 174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2303 2744 3266 5011 5876 6174 2303
Size: 441 522 1745 865 298 3015
Fragments arranged by size:
3015 1745 865 522 441 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5549 882
Size: 617 4050 2219
Fragments arranged by size:
4050 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4677 493
Size: 711 3473 2702
Fragments arranged by size:
3473 2702 711
BsaBI GATnn'nnATC
Cuts at: 6880 6880
Size: 6886
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2916 4693 5075 5710
Size: 53 83 186 936 1383 1777 382 635
Cuts at: 5710 275
Size: 1451
Fragments arranged by size:
1777 1451 1383 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2464 5319 5717 5991 6138 6549 2464
Size: 2855 398 274 147 411 2801
Fragments arranged by size:
2855 2801 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3626 3656 4163 4193 1178
Size: 30 165 30 2223 30 507 30 3871
Fragments arranged by size:
3871 2223 507 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2729 3008 3011 3164 3188 3374 3767 3941
Size: 1543 279 3 153 24 186 393 174
Cuts at: 3941 3988 4009 4138 4141 4144 4156 1186
Size: 47 21 129 3 3 12 3916
Fragments arranged by size:
3916 1543 393 279 186 174 153 129
47 24 21 12 3 3 3
BseYI C'CCAG_C
Cuts at: 2610 2852 6089 6585 2610
Size: 242 3237 496 2911
Fragments arranged by size:
3237 2911 496 242
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2896 3840 1163
Size: 573 1160 944 4209
Fragments arranged by size:
4209 1160 944 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4336 5097 6125 1540
Size: 275 186 2335 761 1028 2301
Fragments arranged by size:
2335 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2829 3189 3735 3780 4947 5032 6103 2829
Size: 360 546 45 1167 85 1071 3612
Fragments arranged by size:
3612 1167 1071 546 360 85 45
BsmI GAATG_Cn'
Cuts at: 2105 2575 4397 4490 2105
Size: 470 1822 93 4501
Fragments arranged by size:
4501 1822 470 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2829 2853 3189 3544 3735 3780 4205
Size: 399 1039 24 336 355 191 45 425
Cuts at: 4205 4947 5032 6103 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1039 742 425 399 355 336
191 85 45 24
BspEI T'CCGG_A
Cuts at: 2464 6549 2464
Size: 4085 2801
BspHI T'CATG_A
Cuts at: 2670 4775 6505 2670
Size: 2105 1730 3051
Fragments arranged by size:
3051 2105 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3672 4241 844
Size: 2828 569 3489
Fragments arranged by size:
3489 2828 569
BsrBI CCG'CTC
Cuts at: 1394 1823 3178 4773 1394
Size: 429 1355 1595 3507
Fragments arranged by size:
3507 1595 1355 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5381 5555 65
Size: 1183 4133 174 1396
Fragments arranged by size:
4133 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5530 5717 1700
Size: 110 3720 187 2869
Fragments arranged by size:
3720 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6886
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6886
BssSI C'ACGA_G
Cuts at: 2630 4946 5958 2630
Size: 2316 1012 3558
Fragments arranged by size:
3558 2316 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6886
BstBI TT'CG_AA
Cuts at: 5690 5690
Size: 6886
BstEII G'GTnAC_C
Cuts at: 1184 3614 1184
Size: 2430 4456
BstXI CCAn_nnnn'nTGG
Cuts at: 2035 2035
Size: 6886
BstYI r'GATC_y
Cuts at: 1972 3363 4216 4583 4968 4985 6437 6523 6535
Size: 1391 853 367 385 17 1452 86 12
Cuts at: 6535 6881 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1391 853 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2991 4156 2991
Size: 1165 5721
BtgI C'CryG_G
Cuts at: 513 1065 3435 513
Size: 552 2370 3964
Fragments arranged by size:
3964 2370 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6147
Fragments arranged by size:
6147 683 56
BtsI GCAGTG_nn'
Cuts at: 2891 4402 5196 5216 2891
Size: 1511 794 20 4561
Fragments arranged by size:
4561 1511 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6886
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6851
DraI TTT'AAA
Cuts at: 2185 4190 2185
Size: 2005 4881
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5893 809
Size: 507 4577 1802
Fragments arranged by size:
4577 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2657 2832
Size: 54 1425 50 275 18 213 614 175
Cuts at: 2832 3129 3747 4333 5222 6851 8
Size: 297 618 586 889 1629 43
Fragments arranged by size:
1629 1425 889 618 614 586 297 275
213 175 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4333 1537
Size: 275 2521 4090
Fragments arranged by size:
4090 2521 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3015 3933 3966 4062 4816 1353
Size: 1662 918 33 96 754 3423
Fragments arranged by size:
3423 1662 918 754 96 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5473 5847 5993 387
Size: 1064 138 3884 374 146 1280
Fragments arranged by size:
3884 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2827 3187 2827
Size: 360 6526
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2217 3531 3692 4023 4126 4949 5546
Size: 393 585 1314 161 331 103 823 597
Cuts at: 5546 6333 6601 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1314 823 787 597 585 393 331
268 161 103
EcoNI CCTnn'n_nnAGG
Cuts at: 2304 2741 2990 2304
Size: 437 249 6200
Fragments arranged by size:
6200 437 249
EcoRI G'AATT_C
Cuts at: 4195 4573 4195
Size: 378 6508
FspI TGC'GCA
Cuts at: 2821 5392 2821
Size: 2571 4315
HaeII r_GCGC'y
Cuts at: 1678 3281 6033 1678
Size: 1603 2752 2531
Fragments arranged by size:
2752 2531 1603
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2054 2086 2667 2699 3626
Size: 32 135 32 482 32 581 32 927
Cuts at: 3626 3658 3682 3714 4109 4141 4163 4195 5527
Size: 32 24 32 395 32 22 32 1332
Cuts at: 5527 5559 5601 5633 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1332 927 581 482 395 135 42
32 32 32 32 32 32 32 32
32 32 24 22
HincII GTy'rAC
Cuts at: 669 1657 1938 4230 4476 669
Size: 988 281 2292 246 3079
Fragments arranged by size:
3079 2292 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6886
HpaI GTT'AAC
Cuts at: 4476 4476
Size: 6886
KpnI G_GTAC'C
Cuts at: 4211 4211
Size: 6886
MfeI C'AATT_G
Cuts at: 4485 4485
Size: 6886
MluI A'CGCG_T
Cuts at: 6762 6762
Size: 6886
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3273 3897 4206 6000 6184 1305
Size: 1968 624 309 1794 184 2007
Fragments arranged by size:
2007 1968 1794 624 309 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2834 10
Size: 54 1425 343 1002 4062
Fragments arranged by size:
4062 1425 1002 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3440 4844 5203 5362 518
Size: 648 2274 1404 359 159 2042
Fragments arranged by size:
2274 2042 1404 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2617 3007 3166 3551 3670 4302 4979 6127
Size: 1011 390 159 385 119 632 677 1148
Cuts at: 6127 6372 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1011 677 632 390 385 245
159 119
NcoI C'CATG_G
Cuts at: 513 1065 3435 513
Size: 552 2370 3964
Fragments arranged by size:
3964 2370 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6886
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6886
NotI GC'GGCC_GC
Cuts at: 4333 4333
Size: 6886
NsiI A_TGCA'T
Cuts at: 2105 2105
Size: 6886
NspI r_CATG'y
Cuts at: 1132 1229 2086 2206 2291 4244 5789 1132
Size: 97 857 120 85 1953 1545 2229
Fragments arranged by size:
2229 1953 1545 857 120 97 85
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6886
PciI A'CATG_T
Cuts at: 2202 2287 5785 2202
Size: 85 3498 3303
Fragments arranged by size:
3498 3303 85
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6886
PmeI GTTT'AAAC
Cuts at: 4190 4190
Size: 6886
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2742 2774 2792 2824 4955 4987 6468 6500 2742
Size: 32 18 32 2131 32 1481 32 3128
Fragments arranged by size:
3128 2131 1481 32 32 32 32 18
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6886
PsiI TTA'TAA
Cuts at: 4456 4456
Size: 6886
PstI C_TGCA'G
Cuts at: 830 3171 3909 4238 830
Size: 2341 738 329 3478
Fragments arranged by size:
3478 2341 738 329
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6886
PvuII CAG'CTG
Cuts at: 2617 3166 3551 2617
Size: 549 385 5952
Fragments arranged by size:
5952 549 385
SacI G_AGCT'C
Cuts at: 2829 3189 2829
Size: 360 6526
SalI G'TCGA_C
Cuts at: 1936 4228 1936
Size: 2292 4594
SbfI CC_TGCA'GG
Cuts at: 4238 4238
Size: 6886
ScaI AGT'ACT
Cuts at: 5134 6846 5134
Size: 1712 5174
SfcI C'TryA_G
Cuts at: 826 1045 3167 3905 4234 5369 6050 6241 826
Size: 219 2122 738 329 1135 681 191 1471
Fragments arranged by size:
2122 1471 1135 738 681 329 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6886
SmaI CCC'GGG
Cuts at: 1872 4213 1872
Size: 2341 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2318 2759 2978 3281 4990 5891
Size: 197 926 375 441 219 303 1709 901
Cuts at: 5891 6153 820
Size: 262 1553
Fragments arranged by size:
1709 1553 926 901 441 375 303 262
219 197
SnaBI TAC'GTA
Cuts at: 493 4677 493
Size: 4184 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6886
SphI G_CATG'C
Cuts at: 1132 4244 1132
Size: 3112 3774
SspI AAT'ATT
Cuts at: 5 52 4810 5
Size: 47 4758 2081
Fragments arranged by size:
4758 2081 47
StuI AGG'CCT
Cuts at: 1517 3425 1517
Size: 1908 4978
StyI C'CwwG_G
Cuts at: 513 1065 2742 3435 4325 513
Size: 552 1677 693 890 3074
Fragments arranged by size:
3074 1677 890 693 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2209 2343 2642 2709 4913 4930 5083
Size: 1255 629 134 299 67 2204 17 153
Cuts at: 5083 5268 5713 325
Size: 185 445 1498
Fragments arranged by size:
2204 1498 1255 629 445 299 185 153
134 67 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2814 3258 3768
Size: 274 80 33 51 1443 837 444 510
Cuts at: 3768 3834 5132 6844 96
Size: 66 1298 1712 138
Fragments arranged by size:
1712 1443 1298 837 510 444 274 138
80 66 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6846 6878 6846
Size: 32 6854
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6886
XbaI T'CTAG_A
Cuts at: 4222 4222
Size: 6886
XhoI C'TCGA_G
Cuts at: 1943 2978 1943
Size: 1035 5851
XmaI C'CCGG_G
Cuts at: 1870 4211 1870
Size: 2341 4545
XmnI GAAnn'nnTTC
Cuts at: 5015 5015
Size: 6886
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4694 276
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstZ17I DraIII EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI