Restriction map for
pFN21AB6257
(Circular) (Six-base) MAPSORT of: pFN21AB6257.seq Check: 2586 from: 1 to: 6994
pFN21AB6257 6994 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3908 4083 3908
Size: 175 6819
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4804 278
Size: 53 83 186 936 3268 2468
Fragments arranged by size:
3268 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4337 1937
Size: 2400 4594
Acc65I G'GTAC_C
Cuts at: 4315 4315
Size: 6994
AclI AA'CG_TT
Cuts at: 1563 4293 5121 5494 1563
Size: 2730 828 373 3063
Fragments arranged by size:
3063 2730 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2899 3309 5057 6441 6709 1632
Size: 1267 410 1748 1384 268 1917
Fragments arranged by size:
1917 1748 1384 1267 410 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6797
AflIII A'CryG_T
Cuts at: 1203 5893 6870 1203
Size: 4690 977 1327
Fragments arranged by size:
4690 1327 977
AgeI A'CCGG_T
Cuts at: 5825 5825
Size: 6994
AhdI GACnn_n'nnGTC
Cuts at: 5723 5723
Size: 6994
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6994
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3045 3938 1184
Size: 1861 893 4240
Fragments arranged by size:
4240 1861 893
AlwNI CAG_nnn'CTG
Cuts at: 2981 3187 3526 3853 4115 4737 6309 2981
Size: 206 339 327 262 622 1572 3666
Fragments arranged by size:
3666 1572 622 339 327 262 206
ApaLI G'TGCA_C
Cuts at: 5051 6207 5051
Size: 1156 5838
ApoI r'AATT_y
Cuts at: 1493 2201 3554 3627 4206 4303 4529 4681 6790
Size: 708 1353 73 579 97 226 152 2109
Cuts at: 6790 1493
Size: 1697
Fragments arranged by size:
2109 1697 1353 708 579 226 152 97
73
AseI AT'TA_AT
Cuts at: 160 1032 2837 5548 160
Size: 872 1805 2711 1606
Fragments arranged by size:
2711 1805 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6994
AvaI C'yCGr_G
Cuts at: 1870 1943 2579 3642 4319 1870
Size: 73 636 1063 677 4545
Fragments arranged by size:
4545 1063 677 636 73
BamHI G'GATC_C
Cuts at: 4324 4691 4324
Size: 367 6627
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4315 5770 618
Size: 325 217 627 2528 1455 1842
Fragments arranged by size:
2528 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2145 2466 4313 1391
Size: 754 321 1847 4072
Fragments arranged by size:
4072 1847 754 321
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2040 2292 928
Size: 971 141 252 5630
Fragments arranged by size:
5630 971 252 141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2529 2563 5185 5219 1903
Size: 34 50 34 508 34 2622 34 3678
Fragments arranged by size:
3678 2622 508 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4886 6096 4886
Size: 1210 5784
BclI T'GATC_A
Cuts at: 1548 2619 2860 1548
Size: 1071 241 5682
Fragments arranged by size:
5682 1071 241
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5605 136
Size: 107 122 71 631 605 3933 1525
Fragments arranged by size:
3933 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6989 6989
Size: 6994
BlpI GC'TnA_GC
Cuts at: 2587 4411 2587
Size: 1824 5170
Bme1580I G_kGCm'C
Cuts at: 1790 5055 6211 1790
Size: 3265 1156 2573
Fragments arranged by size:
3265 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6994
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2487 4725 5683
Size: 416 53 83 399 24 1070 2238 958
Cuts at: 5683 442
Size: 1753
Fragments arranged by size:
2238 1753 1070 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3044 1056
Size: 1988 5006
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3534 3534
Size: 6994
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2783 2892 3045 3527 3630 3875 4059 5654
Size: 1544 109 153 482 103 245 184 1595
Cuts at: 5654 1239
Size: 2579
Fragments arranged by size:
2579 1595 1544 482 245 184 153 109
103
Bpu10I CC'TnA_GC
Cuts at: 3001 3001
Size: 6994
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 5119 5984 6282 4075
Size: 1044 865 298 4787
Fragments arranged by size:
4787 1044 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3473 5657 882
Size: 617 1974 2184 2219
Fragments arranged by size:
2219 2184 1974 617
BsaAI yAC'GTr
Cuts at: 493 1204 4785 493
Size: 711 3581 2702
Fragments arranged by size:
3581 2702 711
BsaBI GATnn'nnATC
Cuts at: 3579 6988 3579
Size: 3409 3585
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4801 5183 5818 275
Size: 53 83 186 936 3268 382 635 1451
Fragments arranged by size:
3268 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5427 5825 6099 6246 6657 5427
Size: 398 274 147 411 5764
Fragments arranged by size:
5764 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4249 4279 1178
Size: 30 165 30 2846 30 3893
Fragments arranged by size:
3893 2846 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 2891 3839 4172 1186
Size: 958 747 948 333 4008
Fragments arranged by size:
4008 958 948 747 333
BseYI C'CCAG_C
Cuts at: 2146 2886 3036 3304 4055 6197 6693 2146
Size: 740 150 268 751 2142 496 2447
Fragments arranged by size:
2447 2142 751 740 496 268 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6421
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4444 5205 6233 1540
Size: 275 186 2443 761 1028 2301
Fragments arranged by size:
2443 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2466 3143 4089 4139 5055 5140 6211 2466
Size: 677 946 50 916 85 1071 3249
Fragments arranged by size:
3249 1071 946 916 677 85 50
BsmI GAATG_Cn'
Cuts at: 3123 3691 4505 4598 3123
Size: 568 814 93 5519
Fragments arranged by size:
5519 814 568 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3842 3842
Size: 6994
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2466 3143 4089 4139 4313 5055
Size: 399 355 321 677 946 50 174 742
Cuts at: 5055 5140 6211 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 946 742 677 399 355 321
174 85 50
BspEI T'CCGG_A
Cuts at: 6657 6657
Size: 6994
BspHI T'CATG_A
Cuts at: 4883 6613 4883
Size: 1730 5264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3043 3908 4083 4349 844
Size: 2199 865 175 266 3489
Fragments arranged by size:
3489 2199 865 266 175
BsrBI CCG'CTC
Cuts at: 1394 1823 4881 1394
Size: 429 3058 3507
Fragments arranged by size:
3507 3058 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2547 3054 3573 3664 4011 5489 5663
Size: 1183 1299 507 519 91 347 1478 174
Cuts at: 5663 65
Size: 1396
Fragments arranged by size:
1478 1396 1299 1183 519 507 347 174
91
BsrFI r'CCGG_y
Cuts at: 1700 1810 5638 5825 1700
Size: 110 3828 187 2869
Fragments arranged by size:
3828 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6994
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6994
BssSI C'ACGA_G
Cuts at: 5054 6066 5054
Size: 1012 5982
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2981 3970 794
Size: 2187 989 3818
Fragments arranged by size:
3818 2187 989
BstBI TT'CG_AA
Cuts at: 5798 5798
Size: 6994
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6994
BstXI CCAn_nnnn'nTGG
Cuts at: 4051 4051
Size: 6994
BstYI r'GATC_y
Cuts at: 1972 2808 3723 3776 4005 4324 4691 5076 5093
Size: 836 915 53 229 319 367 385 17
Cuts at: 5093 6545 6631 6643 6989 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 915 836 385 367 346 319
229 86 53 17 12
Bsu36I CC'TnA_GG
Cuts at: 2028 2028
Size: 6994
BtgI C'CryG_G
Cuts at: 513 1065 2097 2970 4050 513
Size: 552 1032 873 1080 3457
Fragments arranged by size:
3457 1080 1032 873 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4030 487
Size: 683 56 2804 3451
Fragments arranged by size:
3451 2804 683 56
BtsI GCAGTG_nn'
Cuts at: 4017 4510 5304 5324 4017
Size: 493 794 20 5687
Fragments arranged by size:
5687 794 493 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6994
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3568 3603 529
Size: 35 3004 35 3920
Fragments arranged by size:
3920 3004 35 35
DraI TTT'AAA
Cuts at: 4298 4298
Size: 6994
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6001 809
Size: 507 4685 1802
Fragments arranged by size:
4685 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2239 2724 4441
Size: 54 1425 50 275 18 409 485 1717
Cuts at: 4441 5330 6959 8
Size: 889 1629 43
Fragments arranged by size:
1717 1629 1425 889 485 409 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4441 1537
Size: 275 2629 4090
Fragments arranged by size:
4090 2629 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2121 2454 4170 4224 4924 1353
Size: 768 333 1716 54 700 3423
Fragments arranged by size:
3423 1716 768 700 333 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5581 5955 6101 387
Size: 1064 138 3992 374 146 1280
Fragments arranged by size:
3992 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2464 2464
Size: 6994
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2783 2892 2899 3045 3309 3527 3630
Size: 393 1151 109 7 146 264 218 103
Cuts at: 3630 3875 4059 5057 5654 6441 6709 1239
Size: 245 184 998 597 787 268 1524
Fragments arranged by size:
1524 1151 998 787 597 393 268 264
245 218 184 146 109 103 7
EcoO109I rG'GnC_Cy
Cuts at: 3284 3284
Size: 6994
EcoRI G'AATT_C
Cuts at: 4303 4681 4303
Size: 378 6616
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2064 2549 3113 2064
Size: 485 564 5945
Fragments arranged by size:
5945 564 485
FspI TGC'GCA
Cuts at: 3926 3953 5500 3926
Size: 27 1547 5420
Fragments arranged by size:
5420 1547 27
HaeII r_GCGC'y
Cuts at: 1678 6141 1678
Size: 4463 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2360 2392 2886 2918 3209
Size: 32 135 32 788 32 494 32 291
Cuts at: 3209 3241 5635 5667 5709 5741 1373
Size: 32 2394 32 42 32 2626
Fragments arranged by size:
2626 2394 788 494 291 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2434 4338 4584 669
Size: 988 281 496 1904 246 3079
Fragments arranged by size:
3079 1904 988 496 281 246
HindIII A'AGCT_T
Cuts at: 748 2531 748
Size: 1783 5211
HpaI GTT'AAC
Cuts at: 4584 4584
Size: 6994
KpnI G_GTAC'C
Cuts at: 4319 4319
Size: 6994
MfeI C'AATT_G
Cuts at: 4593 4593
Size: 6994
MluI A'CGCG_T
Cuts at: 6870 6870
Size: 6994
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3037 3439 3780 6108 6292 1305
Size: 1732 402 341 2328 184 2007
Fragments arranged by size:
2328 2007 1732 402 341 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2241 2726 10
Size: 54 1425 343 409 485 4278
Fragments arranged by size:
4278 1425 485 409 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2615 4049 4952 5311 5470 518
Size: 648 1449 1434 903 359 159 2042
Fragments arranged by size:
2042 1449 1434 903 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2629 2650 2827 2882 2956 2978 4410 5087
Size: 1023 21 177 55 74 22 1432 677
Cuts at: 5087 6235 6480 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1432 1148 1023 677 245 177 74
55 22 21
NcoI C'CATG_G
Cuts at: 513 1065 2097 2970 4050 513
Size: 552 1032 873 1080 3457
Fragments arranged by size:
3457 1080 1032 873 552
NdeI CA'TA_TG
Cuts at: 387 3252 387
Size: 2865 4129
NheI G'CTAG_C
Cuts at: 1052 3040 1052
Size: 1988 5006
NotI GC'GGCC_GC
Cuts at: 4441 4441
Size: 6994
NspI r_CATG'y
Cuts at: 1132 1229 2171 3748 4352 5897 1132
Size: 97 942 1577 604 1545 2229
Fragments arranged by size:
2229 1577 1545 942 604 97
PasI CC'CwG_GG
Cuts at: 1348 2389 1348
Size: 1041 5953
PciI A'CATG_T
Cuts at: 5893 5893
Size: 6994
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4115 1108
Size: 3007 3987
PfoI T'CCnGG_A
Cuts at: 3061 3061
Size: 6994
PmeI GTTT'AAAC
Cuts at: 4298 4298
Size: 6994
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5063 5095 6576 6608 5063
Size: 32 1481 32 5449
Fragments arranged by size:
5449 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6994
PsiI TTA'TAA
Cuts at: 4564 4564
Size: 6994
PstI C_TGCA'G
Cuts at: 830 2881 3021 4346 830
Size: 2051 140 1325 3478
Fragments arranged by size:
3478 2051 1325 140
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6994
PvuII CAG'CTG
Cuts at: 2629 2650 2827 2882 2956 2978 2629
Size: 21 177 55 74 22 6645
Fragments arranged by size:
6645 177 74 55 22 21
SacI G_AGCT'C
Cuts at: 2466 2466
Size: 6994
SalI G'TCGA_C
Cuts at: 1936 4336 1936
Size: 2400 4594
SapI GCTCTTCn'nnn_
Cuts at: 2454 2454
Size: 6994
SbfI CC_TGCA'GG
Cuts at: 4346 4346
Size: 6994
ScaI AGT'ACT
Cuts at: 3980 5242 6954 3980
Size: 1262 1712 4020
Fragments arranged by size:
4020 1712 1262
SfcI C'TryA_G
Cuts at: 826 1045 2771 2877 3017 4342 5477 6158 6349
Size: 219 1726 106 140 1325 1135 681 191
Cuts at: 6349 826
Size: 1471
Fragments arranged by size:
1726 1471 1325 1135 681 219 191 140
106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6994
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6994
SmaI CCC'GGG
Cuts at: 1872 4321 1872
Size: 2449 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4090 5098 5999 6261 820
Size: 197 926 2147 1008 901 262 1553
Fragments arranged by size:
2147 1553 1008 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4785 493
Size: 4292 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6994
SphI G_CATG'C
Cuts at: 1132 4352 1132
Size: 3220 3774
SspI AAT'ATT
Cuts at: 5 52 4918 5
Size: 47 4866 2081
Fragments arranged by size:
4866 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6994
StyI C'CwwG_G
Cuts at: 513 1065 2097 2970 3088 3097 3375 4050 4433
Size: 552 1032 873 118 9 278 675 383
Cuts at: 4433 513
Size: 3074
Fragments arranged by size:
3074 1032 873 675 552 383 278 118
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3172 3244 3253 5021 5038 5191 5376
Size: 1255 1592 72 9 1768 17 153 185
Cuts at: 5376 5821 325
Size: 445 1498
Fragments arranged by size:
1768 1592 1498 1255 445 185 153 72
17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2381 3876 3978
Size: 274 80 33 51 1443 404 1495 102
Cuts at: 3978 5240 6952 96
Size: 1262 1712 138
Fragments arranged by size:
1712 1495 1443 1262 404 274 138 102
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4249 4281 6954 6986 4249
Size: 32 2673 32 4257
Fragments arranged by size:
4257 2673 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6994
XbaI T'CTAG_A
Cuts at: 4330 4330
Size: 6994
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3095 3095
Size: 6994
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6994
XmaI C'CCGG_G
Cuts at: 1870 4319 1870
Size: 2449 4545
XmnI GAAnn'nnTTC
Cuts at: 3689 5123 3689
Size: 1434 5560
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4802 276
Size: 53 83 186 936 3268 2468
Fragments arranged by size:
3268 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI