Restriction map for
pFN21AB6254
(Circular) (Six-base) MAPSORT of: pFN21AB6254.seq Check: 9911 from: 1 to: 6754
pFN21AB6254 6754 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2697 2740 3490 2697
Size: 43 750 5961
Fragments arranged by size:
5961 750 43
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4564 278
Size: 53 83 186 936 3028 2468
Fragments arranged by size:
3028 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4097 1937
Size: 2160 4594
Acc65I G'GTAC_C
Cuts at: 3594 4075 3594
Size: 481 6273
AclI AA'CG_TT
Cuts at: 1563 4881 5254 1563
Size: 3318 373 3063
Fragments arranged by size:
3318 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2768 4817 6201 6469 1632
Size: 1136 2049 1384 268 1917
Fragments arranged by size:
2049 1917 1384 1136 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6557
AflIII A'CryG_T
Cuts at: 1203 5653 6630 1203
Size: 4450 977 1327
Fragments arranged by size:
4450 1327 977
AgeI A'CCGG_T
Cuts at: 2238 5585 2238
Size: 3347 3407
AhdI GACnn_n'nnGTC
Cuts at: 5483 5483
Size: 6754
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6754
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2735 1184
Size: 1551 5203
AlwNI CAG_nnn'CTG
Cuts at: 2850 3404 3482 3575 4497 6069 2850
Size: 554 78 93 922 1572 3535
Fragments arranged by size:
3535 1572 922 554 93 78
ApaI G_GGCC'C
Cuts at: 2116 3018 3286 2116
Size: 902 268 5584
Fragments arranged by size:
5584 902 268
ApaLI G'TGCA_C
Cuts at: 4811 5967 4811
Size: 1156 5598
ApoI r'AATT_y
Cuts at: 1493 2963 4063 4289 4441 6550 1493
Size: 1470 1100 226 152 2109 1697
Fragments arranged by size:
2109 1697 1470 1100 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5308 160
Size: 872 4276 1606
Fragments arranged by size:
4276 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 2178 2246 2255 2836 3000 3009
Size: 73 129 106 68 9 581 164 9
Cuts at: 3009 3089 3286 3900 4079 1870
Size: 80 197 614 179 4545
Fragments arranged by size:
4545 614 581 197 179 164 129 106
80 73 68 9 9
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2875 2908 2875
Size: 33 6721
BamHI G'GATC_C
Cuts at: 4084 4451 4084
Size: 367 6387
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2251 2471 2685 3169 3213
Size: 325 217 627 464 220 214 484 44
Cuts at: 3213 3594 3850 4075 5530 618
Size: 381 256 225 1455 1842
Fragments arranged by size:
1842 1455 627 484 464 381 325 256
225 220 217 214 44
BanII G_rGCy'C
Cuts at: 1391 2116 2455 2517 3009 3018 3286 3318 3424
Size: 725 339 62 492 9 268 32 106
Cuts at: 3424 3453 4073 1391
Size: 29 620 4072
Fragments arranged by size:
4072 725 620 492 339 268 106 62
32 29 9
BbeI G_GCGC'C
Cuts at: 3217 3217
Size: 6754
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3988 928
Size: 971 2089 3694
Fragments arranged by size:
3694 2089 971
BbvCI CC'TCA_GC
Cuts at: 2366 2366
Size: 6754
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2050 2084 4945 4979 1903
Size: 34 113 34 2861 34 3678
Fragments arranged by size:
3678 2861 113 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4646 5856 4646
Size: 1210 5544
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6754
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2021 3013
Size: 107 122 71 631 605 337 12 992
Cuts at: 3013 3800 3964 5365 136
Size: 787 164 1401 1525
Fragments arranged by size:
1525 1401 992 787 631 605 337 164
122 107 71 12
BglII A'GATC_T
Cuts at: 6749 6749
Size: 6754
BlpI GC'TnA_GC
Cuts at: 3040 4171 3040
Size: 1131 5623
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2116 2256 3018 3172 3286 3753 3768
Size: 283 43 140 762 154 114 467 15
Cuts at: 3768 3796 3896 4815 5971 1790
Size: 28 100 919 1156 2573
Fragments arranged by size:
2573 1156 919 762 467 283 154 140
114 100 43 28 15
BmgBI CAC'GTC
Cuts at: 1318 2500 1318
Size: 1182 5572
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2657 4485 5443
Size: 416 53 83 399 24 1240 1828 958
Cuts at: 5443 442
Size: 1753
Fragments arranged by size:
1828 1753 1240 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6754
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3055 3055
Size: 6754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2064 2303 3872 3941 5414 1239
Size: 825 239 1569 69 1473 2579
Fragments arranged by size:
2579 1569 1473 825 239 69
Bpu10I CC'TnA_GC
Cuts at: 2291 2366 3317 3553 2291
Size: 75 951 236 5492
Fragments arranged by size:
5492 951 236 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3339 3668 3782 4879 5744 6042 3339
Size: 329 114 1097 865 298 4051
Fragments arranged by size:
4051 1097 865 329 298 114
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2082 3045 3505 3577 5417 882
Size: 617 583 963 460 72 1840 2219
Fragments arranged by size:
2219 1840 963 617 583 460 72
BsaAI yAC'GTr
Cuts at: 493 1204 4003 4545 493
Size: 711 2799 542 2702
Fragments arranged by size:
2799 2702 711 542
BsaBI GATnn'nnATC
Cuts at: 6748 6748
Size: 6754
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3214 4561 4943 5578
Size: 53 83 186 936 1681 1347 382 635
Cuts at: 5578 275
Size: 1451
Fragments arranged by size:
1681 1451 1347 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2238 2571 2982 5187 5585 5859 6006 6417 2238
Size: 333 411 2205 398 274 147 411 2575
Fragments arranged by size:
2575 2205 411 411 398 333 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2086 2116 2636 2666 3042
Size: 30 165 30 683 30 520 30 376
Cuts at: 3042 3072 1178
Size: 30 4860
Fragments arranged by size:
4860 683 520 376 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2135 3134 3203 3218 3529 3875 1186
Size: 874 75 999 69 15 311 346 4065
Fragments arranged by size:
4065 999 874 346 311 75 69 15
BseYI C'CCAG_C
Cuts at: 2326 2446 2605 5957 6453 2326
Size: 120 159 3352 496 2627
Fragments arranged by size:
3352 2627 496 159 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2124 3080 1163
Size: 573 388 956 4837
Fragments arranged by size:
4837 956 573 388
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 3740 4204 4965 5993 1540
Size: 275 186 15 1724 464 761 1028 2301
Fragments arranged by size:
2301 1724 1028 761 464 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 2517 3872 4815 4900 5971 2517
Size: 1355 943 85 1071 3300
Fragments arranged by size:
3300 1355 1071 943 85
BsmI GAATG_Cn'
Cuts at: 4265 4358 4265
Size: 93 6661
BsmBI CGTCTCn'nnnn_
Cuts at: 2089 2656 2089
Size: 567 6187
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2116 2256 2455 2517 3009 3018
Size: 399 283 43 140 199 62 492 9
Cuts at: 3018 3172 3286 3318 3424 3453 3753 3768 3796
Size: 154 114 32 106 29 300 15 28
Cuts at: 3796 3872 3896 4073 4815 4900 5971 1391
Size: 76 24 177 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 492 399 300 283 199
177 154 140 114 106 85 76 62
43 32 29 28 24 15 9
BspEI T'CCGG_A
Cuts at: 2571 2982 6417 2571
Size: 411 3435 2908
Fragments arranged by size:
3435 2908 411
BspHI T'CATG_A
Cuts at: 4643 6373 4643
Size: 1730 5024
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2683 2697 2740 3490 4109 844
Size: 1839 14 43 750 619 3489
Fragments arranged by size:
3489 1839 750 619 43 14
BsrBI CCG'CTC
Cuts at: 1394 1823 2110 3208 3244 3270 4641 1394
Size: 429 287 1098 36 26 1371 3507
Fragments arranged by size:
3507 1371 1098 429 287 36 26
BsrDI GCAATG_nn'
Cuts at: 65 1248 5249 5423 65
Size: 1183 4001 174 1396
Fragments arranged by size:
4001 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2157 2238 3193 5398 5585 1700
Size: 110 347 81 955 2205 187 2869
Fragments arranged by size:
2869 2205 955 347 187 110 81
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6754
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6754
BssSI C'ACGA_G
Cuts at: 4814 5826 4814
Size: 1012 5742
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3404 794
Size: 2610 4144
BstBI TT'CG_AA
Cuts at: 5558 5558
Size: 6754
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6754
BstYI r'GATC_y
Cuts at: 1972 2863 2985 3880 4084 4451 4836 4853 6305
Size: 891 122 895 204 367 385 17 1452
Cuts at: 6305 6391 6403 6749 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 895 891 385 367 346 204
122 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2417 2681 3675 3855 2417
Size: 264 994 180 5316
Fragments arranged by size:
5316 994 264 180
BtgI C'CryG_G
Cuts at: 513 1065 2004 2127 3488 513
Size: 552 939 123 1361 3779
Fragments arranged by size:
3779 1361 939 552 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6015
Fragments arranged by size:
6015 683 56
BtsI GCAGTG_nn'
Cuts at: 2107 3894 4270 5064 5084 2107
Size: 1787 376 794 20 3777
Fragments arranged by size:
3777 1787 794 376 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6754
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6719
DraI TTT'AAA
Cuts at: 4058 4058
Size: 6754
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2567 3083 5761 809
Size: 507 1251 516 2678 1802
Fragments arranged by size:
2678 1802 1251 516 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2130 3491
Size: 54 1425 50 275 18 183 117 1361
Cuts at: 3491 3684 3696 3723 3737 4201 5090 6719 8
Size: 193 12 27 14 464 889 1629 43
Fragments arranged by size:
1629 1425 1361 889 464 275 193 183
117 54 50 43 27 18 14 12
EagI C'GGCC_G
Cuts at: 1537 1812 2013 3737 4201 1537
Size: 275 201 1724 464 4090
Fragments arranged by size:
4090 1724 464 275 201
EarI CTCTTCn'nnn_
Cuts at: 1353 2554 2595 3096 3533 4684 1353
Size: 1201 41 501 437 1151 3423
Fragments arranged by size:
3423 1201 1151 501 437 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2615 5341 5715 5861 387
Size: 1064 138 1026 2726 374 146 1280
Fragments arranged by size:
2726 1280 1064 1026 374 146 138
EcoICRI GAG'CTC
Cuts at: 2515 2515
Size: 6754
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2064 2303 2768 3872 3941 4817 5414
Size: 393 432 239 465 1104 69 876 597
Cuts at: 5414 6201 6469 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1104 876 787 597 465 432 393
268 239 69
EcoNI CCTnn'n_nnAGG
Cuts at: 2120 3150 3674 3686 2120
Size: 1030 524 12 5188
Fragments arranged by size:
5188 1030 524 12
EcoO109I rG'GnC_Cy
Cuts at: 2113 2139 2287 2422 2560 2611 2815 3014 3282
Size: 26 148 135 138 51 204 199 268
Cuts at: 3282 3283 3557 3643 3679 3949 2113
Size: 1 274 86 36 270 4918
Fragments arranged by size:
4918 274 270 268 204 199 148 138
135 86 51 36 26 1
EcoRI G'AATT_C
Cuts at: 4063 4441 4063
Size: 378 6376
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3470 3470
Size: 6754
FspI TGC'GCA
Cuts at: 5260 5260
Size: 6754
HaeII r_GCGC'y
Cuts at: 1678 2164 3217 5901 1678
Size: 486 1053 2684 2531
Fragments arranged by size:
2684 2531 1053 486
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2504 2536 2830 2862 3854
Size: 32 135 32 932 32 294 32 992
Cuts at: 3854 3886 5395 5427 5469 5501 1373
Size: 32 1509 32 42 32 2626
Fragments arranged by size:
2626 1509 992 932 294 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2955 4098 4344 669
Size: 988 281 1017 1143 246 3079
Fragments arranged by size:
3079 1143 1017 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6754
HpaI GTT'AAC
Cuts at: 4344 4344
Size: 6754
KasI G'GCGC_C
Cuts at: 3213 3213
Size: 6754
KpnI G_GTAC'C
Cuts at: 3598 4079 3598
Size: 481 6273
MfeI C'AATT_G
Cuts at: 2305 4353 2305
Size: 2048 4706
MluI A'CGCG_T
Cuts at: 6630 6630
Size: 6754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5868 6052 1305
Size: 4563 184 2007
Fragments arranged by size:
4563 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2132 3698 3725 10
Size: 54 1425 343 300 1566 27 3039
Fragments arranged by size:
3039 1566 1425 343 300 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 3761 4712 5071 5230 518
Size: 648 2595 951 359 159 2042
Fragments arranged by size:
2595 2042 951 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2272 2380 2410 3044 3232 3410 3634 3664
Size: 666 108 30 634 188 178 224 30
Cuts at: 3664 3721 4170 4847 5995 6240 1606
Size: 57 449 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 666 634 449 245 224
188 178 108 57 30 30
NaeI GCC'GGC
Cuts at: 3195 3195
Size: 6754
NarI GG'CG_CC
Cuts at: 3214 3214
Size: 6754
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5263
Fragments arranged by size:
5263 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6754
NgoMIV G'CCGG_C
Cuts at: 3193 3193
Size: 6754
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6754
NotI GC'GGCC_GC
Cuts at: 4201 4201
Size: 6754
NspI r_CATG'y
Cuts at: 1132 1229 4112 5657 1132
Size: 97 2883 1545 2229
Fragments arranged by size:
2883 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2476 2809 3146 1348
Size: 1128 333 337 4956
Fragments arranged by size:
4956 1128 337 333
PciI A'CATG_T
Cuts at: 5653 5653
Size: 6754
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6754
PfoI T'CCnGG_A
Cuts at: 2231 2872 2231
Size: 641 6113
PmeI GTTT'AAAC
Cuts at: 4058 4058
Size: 6754
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4823 4855 6336 6368 4823
Size: 32 1481 32 5209
Fragments arranged by size:
5209 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2139 2422 2560 2611 2815 3643 3679 2139
Size: 283 138 51 204 828 36 5214
Fragments arranged by size:
5214 828 283 204 138 51 36
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6754
PsiI TTA'TAA
Cuts at: 4324 4324
Size: 6754
PspOMI G'GGCC_C
Cuts at: 2112 3014 3282 2112
Size: 902 268 5584
Fragments arranged by size:
5584 902 268
PstI C_TGCA'G
Cuts at: 830 2694 2724 2860 3409 3663 4106 830
Size: 1864 30 136 549 254 443 3478
Fragments arranged by size:
3478 1864 549 443 254 136 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6754
PvuII CAG'CTG
Cuts at: 2272 2380 2410 3232 3634 3721 2272
Size: 108 30 822 402 87 5305
Fragments arranged by size:
5305 822 402 108 87 30
RsrII CG'GwC_CG
Cuts at: 3912 3912
Size: 6754
SacI G_AGCT'C
Cuts at: 2517 2517
Size: 6754
SalI G'TCGA_C
Cuts at: 1936 4096 1936
Size: 2160 4594
SanDI GG'GwC_CC
Cuts at: 2139 2139
Size: 6754
SapI GCTCTTCn'nnn_
Cuts at: 2595 3096 2595
Size: 501 6253
SbfI CC_TGCA'GG
Cuts at: 2694 4106 2694
Size: 1412 5342
ScaI AGT'ACT
Cuts at: 5002 6714 5002
Size: 1712 5042
SfcI C'TryA_G
Cuts at: 826 1045 2690 2720 2856 3405 3659 4102 5237
Size: 219 1645 30 136 549 254 443 1135
Cuts at: 5237 5918 6109 826
Size: 681 191 1471
Fragments arranged by size:
1645 1471 1135 681 549 443 254 219
191 136 30
SfoI GGC'GCC
Cuts at: 3215 3215
Size: 6754
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6754
SmaI CCC'GGG
Cuts at: 1872 2074 2180 3002 3011 3091 3902 4081 1872
Size: 202 106 822 9 80 811 179 4545
Fragments arranged by size:
4545 822 811 202 179 106 80 9
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3354 3647 3797 4858 5759 6021
Size: 197 926 1411 293 150 1061 901 262
Cuts at: 6021 820
Size: 1553
Fragments arranged by size:
1553 1411 1061 926 901 293 262 197
150
SnaBI TAC'GTA
Cuts at: 493 4545 493
Size: 4052 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6754
SphI G_CATG'C
Cuts at: 1132 4112 1132
Size: 2980 3774
SspI AAT'ATT
Cuts at: 5 52 4678 5
Size: 47 4626 2081
Fragments arranged by size:
4626 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6754
StyI C'CwwG_G
Cuts at: 513 1065 2004 2331 4193 513
Size: 552 939 327 1862 3074
Fragments arranged by size:
3074 1862 939 552 327
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2231 4781 4798 4951 5136 5581 325
Size: 1255 651 2550 17 153 185 445 1498
Fragments arranged by size:
2550 1498 1255 651 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5000 6712 96
Size: 274 80 33 51 1443 3023 1712 138
Fragments arranged by size:
3023 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2086 2118 3042 3074 6714 6746 2086
Size: 32 924 32 3640 32 2094
Fragments arranged by size:
3640 2094 924 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3575 1316
Size: 2259 4495
XbaI T'CTAG_A
Cuts at: 3826 4090 3826
Size: 264 6490
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6754
XmaI C'CCGG_G
Cuts at: 1870 2072 2178 3000 3009 3089 3900 4079 1870
Size: 202 106 822 9 80 811 179 4545
Fragments arranged by size:
4545 822 811 202 179 106 80 9
XmnI GAAnn'nnTTC
Cuts at: 2171 4883 2171
Size: 2712 4042
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4562 276
Size: 53 83 186 936 3028 2468
Fragments arranged by size:
3028 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BsiWI BstXI BstZ17I
DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI SacII SexAI SfiI SrfI SwaI XcmI