Restriction map for
pFN21AB6248
(Circular) (Six-base) MAPSORT of: pFN21AB6248.seq Check: 5256 from: 1 to: 6748
pFN21AB6248 6748 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2428 2760 3238 2428
Size: 332 478 5938
Fragments arranged by size:
5938 478 332
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4558 278
Size: 53 83 186 936 3022 2468
Fragments arranged by size:
3022 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4091 1937
Size: 2154 4594
Acc65I G'GTAC_C
Cuts at: 4069 4069
Size: 6748
AclI AA'CG_TT
Cuts at: 1563 4875 5248 1563
Size: 3312 373 3063
Fragments arranged by size:
3312 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4811 6195 6463 1632
Size: 3179 1384 268 1917
Fragments arranged by size:
3179 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6551
AflIII A'CryG_T
Cuts at: 1203 2624 5647 6624 1203
Size: 1421 3023 977 1327
Fragments arranged by size:
3023 1421 1327 977
AgeI A'CCGG_T
Cuts at: 3940 5579 3940
Size: 1639 5109
AhdI GACnn_n'nnGTC
Cuts at: 5477 5477
Size: 6748
AleI CACnn'nnGTG
Cuts at: 1166 2066 2600 1166
Size: 900 534 5314
Fragments arranged by size:
5314 900 534
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2084 1184
Size: 900 5848
AlwNI CAG_nnn'CTG
Cuts at: 2231 2369 3631 4491 6063 2231
Size: 138 1262 860 1572 2916
Fragments arranged by size:
2916 1572 1262 860 138
ApaLI G'TGCA_C
Cuts at: 2843 3011 4805 5961 2843
Size: 168 1794 1156 3630
Fragments arranged by size:
3630 1794 1156 168
ApoI r'AATT_y
Cuts at: 1493 2049 2303 2505 4057 4283 4435 6544 1493
Size: 556 254 202 1552 226 152 2109 1697
Fragments arranged by size:
2109 1697 1552 556 254 226 202 152
AseI AT'TA_AT
Cuts at: 160 1032 5302 160
Size: 872 4270 1606
Fragments arranged by size:
4270 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6748
AvaI C'yCGr_G
Cuts at: 1870 1943 2848 4073 1870
Size: 73 905 1225 4545
Fragments arranged by size:
4545 1225 905 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2908 2941 3076 3109 2908
Size: 33 135 33 6547
Fragments arranged by size:
6547 135 33 33
BamHI G'GATC_C
Cuts at: 2673 4078 4445 2673
Size: 1405 367 4976
Fragments arranged by size:
4976 1405 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2589 3448 3517 4069 5524
Size: 325 217 627 802 859 69 552 1455
Cuts at: 5524 618
Size: 1842
Fragments arranged by size:
1842 1455 859 802 627 552 325 217
69
BanII G_rGCy'C
Cuts at: 1391 2042 4067 1391
Size: 651 2025 4072
Fragments arranged by size:
4072 2025 651
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3937 928
Size: 971 2038 3739
Fragments arranged by size:
3739 2038 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4939 4973 1903
Size: 34 3002 34 3678
Fragments arranged by size:
3678 3002 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3104 4640 5850 3104
Size: 1536 1210 4002
Fragments arranged by size:
4002 1536 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6748
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2598 3584 5359
Size: 107 122 71 631 605 926 986 1775
Cuts at: 5359 136
Size: 1525
Fragments arranged by size:
1775 1525 986 926 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2706 6743 2706
Size: 4037 2711
BlpI GC'TnA_GC
Cuts at: 4165 4165
Size: 6748
Bme1580I G_kGCm'C
Cuts at: 1790 2847 3015 3453 3474 3522 4809 5965 1790
Size: 1057 168 438 21 48 1287 1156 2573
Fragments arranged by size:
2573 1287 1156 1057 438 168 48 21
BmgBI CAC'GTC
Cuts at: 1318 2625 3376 1318
Size: 1307 751 4690
Fragments arranged by size:
4690 1307 751
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3469 4479 5437
Size: 416 53 83 399 24 2052 1010 958
Cuts at: 5437 442
Size: 1753
Fragments arranged by size:
2052 1753 1010 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6748
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3055 3055
Size: 6748
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2283 2716 3046 3371 3428 3440 5408 1239
Size: 1044 433 330 325 57 12 1968 2579
Fragments arranged by size:
2579 1968 1044 433 330 325 57 12
Bpu10I CC'TnA_GC
Cuts at: 3161 3161
Size: 6748
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2894 4873 5738 6036 2894
Size: 1979 865 298 3606
Fragments arranged by size:
3606 1979 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2267 5411 882
Size: 617 768 3144 2219
Fragments arranged by size:
3144 2219 768 617
BsaAI yAC'GTr
Cuts at: 493 1204 4539 493
Size: 711 3335 2702
Fragments arranged by size:
3335 2702 711
BsaBI GATnn'nnATC
Cuts at: 6742 6742
Size: 6748
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2772 4555 4937 5572
Size: 53 83 186 936 1239 1783 382 635
Cuts at: 5572 275
Size: 1451
Fragments arranged by size:
1783 1451 1239 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2994 3940 5181 5579 5853 6000 6411 2994
Size: 946 1241 398 274 147 411 3331
Fragments arranged by size:
3331 1241 946 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3044 3074 1178
Size: 30 165 30 1641 30 4852
Fragments arranged by size:
4852 1641 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3940 3943 1186
Size: 2754 3 3991
Fragments arranged by size:
3991 2754 3
BseYI C'CCAG_C
Cuts at: 2195 3152 3404 3461 3699 3960 5951 6447 2195
Size: 957 252 57 238 261 1991 496 2496
Fragments arranged by size:
2496 1991 957 496 261 252 238 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2875 3085 3734 1163
Size: 573 1139 210 649 4177
Fragments arranged by size:
4177 1139 649 573 210
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 4198 4959 5987 1540
Size: 275 186 1108 1089 761 1028 2301
Fragments arranged by size:
2301 1108 1089 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2847 3015 3041 4809 4894 5965 2847
Size: 168 26 1768 85 1071 3630
Fragments arranged by size:
3630 1768 1071 168 85 26
BsmI GAATG_Cn'
Cuts at: 3050 4259 4352 3050
Size: 1209 93 5446
Fragments arranged by size:
5446 1209 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2847 3015 3041 3453 3474 3522
Size: 399 252 805 168 26 412 21 48
Cuts at: 3522 4067 4809 4894 5965 1391
Size: 545 742 85 1071 2174
Fragments arranged by size:
2174 1071 805 742 545 412 399 252
168 85 48 26 21
BspEI T'CCGG_A
Cuts at: 2994 6411 2994
Size: 3417 3331
BspHI T'CATG_A
Cuts at: 4637 6367 4637
Size: 1730 5018
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2343 2428 2760 3238 4103 844
Size: 1499 85 332 478 865 3489
Fragments arranged by size:
3489 1499 865 478 332 85
BsrBI CCG'CTC
Cuts at: 1394 1823 2494 2689 4635 1394
Size: 429 671 195 1946 3507
Fragments arranged by size:
3507 1946 671 429 195
BsrDI GCAATG_nn'
Cuts at: 65 1248 2887 2959 3699 5243 5417 65
Size: 1183 1639 72 740 1544 174 1396
Fragments arranged by size:
1639 1544 1396 1183 740 174 72
BsrFI r'CCGG_y
Cuts at: 1700 1810 3227 3243 3618 3703 3940 5392 5579
Size: 110 1417 16 375 85 237 1452 187
Cuts at: 5579 1700
Size: 2869
Fragments arranged by size:
2869 1452 1417 375 237 187 110 85
16
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6748
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6748
BssSI C'ACGA_G
Cuts at: 3086 3725 4808 5820 3086
Size: 639 1083 1012 4014
Fragments arranged by size:
4014 1083 1012 639
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3129 3517 3631 794
Size: 2335 388 114 3911
Fragments arranged by size:
3911 2335 388 114
BstBI TT'CG_AA
Cuts at: 5552 5552
Size: 6748
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6748
BstYI r'GATC_y
Cuts at: 1972 2673 2706 3627 4078 4445 4830 4847 6299
Size: 701 33 921 451 367 385 17 1452
Cuts at: 6299 6385 6397 6743 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 921 701 451 385 367 346
86 33 17 12
Bsu36I CC'TnA_GG
Cuts at: 2669 3775 2669
Size: 1106 5642
BtgI C'CryG_G
Cuts at: 513 1065 2340 3184 3768 513
Size: 552 1275 844 584 3493
Fragments arranged by size:
3493 1275 844 584 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3309 3901 3915 487
Size: 683 56 2083 592 14 3320
Fragments arranged by size:
3320 2083 683 592 56 14
BtsI GCAGTG_nn'
Cuts at: 3097 3280 3564 3927 4264 5058 5078 3097
Size: 183 284 363 337 794 20 4767
Fragments arranged by size:
4767 794 363 337 284 183 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6748
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2140 2175 529
Size: 35 1576 35 5102
Fragments arranged by size:
5102 1576 35 35
DraI TTT'AAA
Cuts at: 2502 4052 2502
Size: 1550 5198
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5755 809
Size: 507 4439 1802
Fragments arranged by size:
4439 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3252 3924 4195
Size: 54 1425 50 275 18 1422 672 271
Cuts at: 4195 5084 6713 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1422 889 672 275 271 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4195 1537
Size: 275 2383 4090
Fragments arranged by size:
4090 2383 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3266 3484 3961 4678 1353
Size: 1913 218 477 717 3423
Fragments arranged by size:
3423 1913 717 477 218
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5335 5709 5855 387
Size: 1064 138 3746 374 146 1280
Fragments arranged by size:
3746 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 2716 3046 3371 3428 3440 4811
Size: 393 651 433 330 325 57 12 1371
Cuts at: 4811 5408 6195 6463 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1371 787 651 597 433 393 330
325 268 57 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3635 3635
Size: 6748
EcoRI G'AATT_C
Cuts at: 4057 4435 4057
Size: 378 6370
FspI TGC'GCA
Cuts at: 3080 5254 3080
Size: 2174 4574
HaeII r_GCGC'y
Cuts at: 1678 2802 3938 5895 1678
Size: 1124 1136 1957 2531
Fragments arranged by size:
2531 1957 1136 1124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3193 3225 3483 3515 5389
Size: 32 135 32 1621 32 258 32 1874
Cuts at: 5389 5421 5463 5495 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1874 1621 258 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4092 4338 669
Size: 988 281 2154 246 3079
Fragments arranged by size:
3079 2154 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6748
HpaI GTT'AAC
Cuts at: 4338 4338
Size: 6748
KpnI G_GTAC'C
Cuts at: 4073 4073
Size: 6748
MfeI C'AATT_G
Cuts at: 4347 4347
Size: 6748
MluI A'CGCG_T
Cuts at: 6624 6624
Size: 6748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3000 5862 6046 1305
Size: 1695 2862 184 2007
Fragments arranged by size:
2862 2007 1695 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3254 3926 10
Size: 54 1425 343 1422 672 2832
Fragments arranged by size:
2832 1425 1422 672 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2066 2532 2600 2840 3920 4706 5065
Size: 648 900 466 68 240 1080 786 359
Cuts at: 5065 5224 518
Size: 159 2042
Fragments arranged by size:
2042 1080 900 786 648 466 359 240
159 68
MspA1I CmG'CkG
Cuts at: 1606 2170 2639 2889 2937 3554 4164 4841 5989
Size: 564 469 250 48 617 610 677 1148
Cuts at: 5989 6234 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 617 610 564 469 250
245 48
NaeI GCC'GGC
Cuts at: 3245 3620 3705 3245
Size: 375 85 6288
Fragments arranged by size:
6288 375 85
NcoI C'CATG_G
Cuts at: 513 1065 3184 513
Size: 552 2119 4077
Fragments arranged by size:
4077 2119 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6748
NgoMIV G'CCGG_C
Cuts at: 3243 3618 3703 3243
Size: 375 85 6288
Fragments arranged by size:
6288 375 85
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6748
NotI GC'GGCC_GC
Cuts at: 4195 4195
Size: 6748
NspI r_CATG'y
Cuts at: 1132 1229 2587 2839 4106 5651 1132
Size: 97 1358 252 1267 1545 2229
Fragments arranged by size:
2229 1545 1358 1267 252 97
PasI CC'CwG_GG
Cuts at: 1348 3608 1348
Size: 2260 4488
PciI A'CATG_T
Cuts at: 5647 5647
Size: 6748
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6748
PmeI GTTT'AAAC
Cuts at: 4052 4052
Size: 6748
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4817 4849 6330 6362 4817
Size: 32 1481 32 5203
Fragments arranged by size:
5203 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6748
PsiI TTA'TAA
Cuts at: 4318 4318
Size: 6748
PstI C_TGCA'G
Cuts at: 830 3134 3559 3627 4100 830
Size: 2304 425 68 473 3478
Fragments arranged by size:
3478 2304 473 425 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6748
PvuII CAG'CTG
Cuts at: 2170 2170
Size: 6748
SalI G'TCGA_C
Cuts at: 1936 4090 1936
Size: 2154 4594
SbfI CC_TGCA'GG
Cuts at: 4100 4100
Size: 6748
ScaI AGT'ACT
Cuts at: 4996 6708 4996
Size: 1712 5036
SfcI C'TryA_G
Cuts at: 826 1045 2794 3130 3555 3623 4096 5231 5912
Size: 219 1749 336 425 68 473 1135 681
Cuts at: 5912 6103 826
Size: 191 1471
Fragments arranged by size:
1749 1471 1135 681 473 425 336 219
191 68
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6748
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6748
SmaI CCC'GGG
Cuts at: 1872 4075 1872
Size: 2203 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2909 4852 5753 6015 820
Size: 197 926 966 1943 901 262 1553
Fragments arranged by size:
1943 1553 966 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4539 493
Size: 4046 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6748
SphI G_CATG'C
Cuts at: 1132 4106 1132
Size: 2974 3774
SspI AAT'ATT
Cuts at: 5 52 4672 5
Size: 47 4620 2081
Fragments arranged by size:
4620 2081 47
StuI AGG'CCT
Cuts at: 1517 3852 1517
Size: 2335 4413
StyI C'CwwG_G
Cuts at: 513 1065 3184 3579 4187 513
Size: 552 2119 395 608 3074
Fragments arranged by size:
3074 2119 608 552 395
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2252 2963 3737 4775 4792 4945 5130
Size: 1255 672 711 774 1038 17 153 185
Cuts at: 5130 5575 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1038 774 711 672 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3351 4994 6706
Size: 274 80 33 51 1443 1374 1643 1712
Cuts at: 6706 96
Size: 138
Fragments arranged by size:
1712 1643 1443 1374 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2741 2773 6708 6740 2741
Size: 32 3935 32 2749
Fragments arranged by size:
3935 2749 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6748
XbaI T'CTAG_A
Cuts at: 3211 4084 3211
Size: 873 5875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3794 3794
Size: 6748
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6748
XmaI C'CCGG_G
Cuts at: 1870 4073 1870
Size: 2203 4545
XmnI GAAnn'nnTTC
Cuts at: 2861 2945 3179 3275 4877 2861
Size: 84 234 96 1602 4732
Fragments arranged by size:
4732 1602 234 96 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4556 276
Size: 53 83 186 936 3022 2468
Fragments arranged by size:
3022 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI