Restriction map for
pFN21AB6247
(Circular) (Six-base) MAPSORT of: pFN21AB6247.seq Check: 9989 from: 1 to: 6967
pFN21AB6247 6967 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2807 2807
Size: 6967
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4777 278
Size: 53 83 186 936 3241 2468
Fragments arranged by size:
3241 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2718 4310 1937
Size: 781 1592 4594
Fragments arranged by size:
4594 1592 781
Acc65I G'GTAC_C
Cuts at: 4288 4288
Size: 6967
AclI AA'CG_TT
Cuts at: 1563 5094 5467 1563
Size: 3531 373 3063
Fragments arranged by size:
3531 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2158 3247 3667 5030 6414 6682 1632
Size: 526 1089 420 1363 1384 268 1917
Fragments arranged by size:
1917 1384 1363 1089 526 420 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6770
AflIII A'CryG_T
Cuts at: 1203 5866 6843 1203
Size: 4663 977 1327
Fragments arranged by size:
4663 1327 977
AgeI A'CCGG_T
Cuts at: 5798 5798
Size: 6967
AhdI GACnn_n'nnGTC
Cuts at: 3545 5696 3545
Size: 2151 4816
AleI CACnn'nnGTG
Cuts at: 1166 2412 3894 3978 1166
Size: 1246 1482 84 4155
Fragments arranged by size:
4155 1482 1246 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6967
AlwNI CAG_nnn'CTG
Cuts at: 2284 2311 2534 2936 3550 4265 4710 6282 2284
Size: 27 223 402 614 715 445 1572 2969
Fragments arranged by size:
2969 1572 715 614 445 402 223 27
ApaLI G'TGCA_C
Cuts at: 2264 5024 6180 2264
Size: 2760 1156 3051
Fragments arranged by size:
3051 2760 1156
ApoI r'AATT_y
Cuts at: 1493 2026 2861 3801 3969 4276 4502 4654 6763
Size: 533 835 940 168 307 226 152 2109
Cuts at: 6763 1493
Size: 1697
Fragments arranged by size:
2109 1697 940 835 533 307 226 168
152
AseI AT'TA_AT
Cuts at: 160 1032 5521 160
Size: 872 4489 1606
Fragments arranged by size:
4489 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6967
AvaI C'yCGr_G
Cuts at: 1870 1943 4292 1870
Size: 73 2349 4545
Fragments arranged by size:
4545 2349 73
BamHI G'GATC_C
Cuts at: 4297 4664 4297
Size: 367 6600
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2073 3876 4288 5743 618
Size: 325 217 627 286 1803 412 1455 1842
Fragments arranged by size:
1842 1803 1455 627 412 325 286 217
BanII G_rGCy'C
Cuts at: 1391 2238 2334 2528 2542 2767 4286 1391
Size: 847 96 194 14 225 1519 4072
Fragments arranged by size:
4072 1519 847 225 194 96 14
BbeI G_GCGC'C
Cuts at: 2077 2077
Size: 6967
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3096 3263 928
Size: 971 1197 167 4632
Fragments arranged by size:
4632 1197 971 167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5158 5192 1903
Size: 34 3221 34 3678
Fragments arranged by size:
3678 3221 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2683 4859 6069 2683
Size: 2176 1210 3581
Fragments arranged by size:
3581 2176 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6967
BfrBI ATG'CAT
Cuts at: 2458 2458
Size: 6967
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2813 5578 136
Size: 107 122 71 631 605 1141 2765 1525
Fragments arranged by size:
2765 1525 1141 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6962 6962
Size: 6967
BlpI GC'TnA_GC
Cuts at: 2527 4384 2527
Size: 1857 5110
Bme1580I G_kGCm'C
Cuts at: 1790 2268 5028 6184 1790
Size: 478 2760 1156 2573
Fragments arranged by size:
2760 2573 1156 478
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6967
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2577 3483 3651
Size: 416 53 83 399 24 1160 906 168
Cuts at: 3651 3735 4071 4698 5656 442
Size: 84 336 627 958 1753
Fragments arranged by size:
1753 1160 958 906 627 416 399 336
168 84 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6967
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4258 4258
Size: 6967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2306 2556 2567 2595 2958 3243 3831
Size: 875 192 250 11 28 363 285 588
Cuts at: 3831 4015 4251 5627 1239
Size: 184 236 1376 2579
Fragments arranged by size:
2579 1376 875 588 363 285 250 236
192 184 28 11
Bpu10I CC'TnA_GC
Cuts at: 2312 2312
Size: 6967
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2321 2613 5092 5957 6255 2321
Size: 292 2479 865 298 3033
Fragments arranged by size:
3033 2479 865 298 292
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2640 5630 882
Size: 617 1141 2990 2219
Fragments arranged by size:
2990 2219 1141 617
BsaAI yAC'GTr
Cuts at: 493 1204 4758 493
Size: 711 3554 2702
Fragments arranged by size:
3554 2702 711
BsaBI GATnn'nnATC
Cuts at: 6961 6961
Size: 6967
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2074 4774 5156 5791
Size: 53 83 186 936 541 2700 382 635
Cuts at: 5791 275
Size: 1451
Fragments arranged by size:
2700 1451 936 635 541 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2334 4145 5400 5798 6072 6219 6630 2334
Size: 1811 1255 398 274 147 411 2671
Fragments arranged by size:
2671 1811 1255 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2562 2592 3682 3712 1178
Size: 30 165 30 1159 30 1090 30 4433
Fragments arranged by size:
4433 1159 1090 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2387 2438 2480 2490 1186
Size: 1201 51 42 10 5663
Fragments arranged by size:
5663 1201 51 42 10
BseYI C'CCAG_C
Cuts at: 2437 3276 6170 6666 2437
Size: 839 2894 496 2738
Fragments arranged by size:
2894 2738 839 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2366 3536 1163
Size: 573 630 1170 4594
Fragments arranged by size:
4594 1170 630 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4417 5178 6206 1540
Size: 275 186 2416 761 1028 2301
Fragments arranged by size:
2416 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2051 2238 2268 2334 5028 5113 6184 2051
Size: 187 30 66 2694 85 1071 2834
Fragments arranged by size:
2834 2694 1071 187 85 66 30
BsmI GAATG_Cn'
Cuts at: 3329 3497 3512 3596 4478 4571 3329
Size: 168 15 84 882 93 5725
Fragments arranged by size:
5725 882 168 93 84 15
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2238 2268 2334 2528 2542 2767
Size: 399 261 187 30 66 194 14 225
Cuts at: 2767 4286 5028 5113 6184 1391
Size: 1519 742 85 1071 2174
Fragments arranged by size:
2174 1519 1071 742 399 261 225 194
187 85 66 30 14
BspEI T'CCGG_A
Cuts at: 2334 6630 2334
Size: 4296 2671
BspHI T'CATG_A
Cuts at: 4856 6586 4856
Size: 1730 5237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2807 4322 844
Size: 1963 1515 3489
Fragments arranged by size:
3489 1963 1515
BsrBI CCG'CTC
Cuts at: 1394 1823 4854 1394
Size: 429 3031 3507
Fragments arranged by size:
3507 3031 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3502 3586 3922 4090 5462 5636 65
Size: 1183 2254 84 336 168 1372 174 1396
Fragments arranged by size:
2254 1396 1372 1183 336 174 168 84
BsrFI r'CCGG_y
Cuts at: 1700 1810 2223 5611 5798 1700
Size: 110 413 3388 187 2869
Fragments arranged by size:
3388 2869 413 187 110
BsrGI T'GTAC_A
Cuts at: 96 4126 96
Size: 4030 2937
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6967
BssSI C'ACGA_G
Cuts at: 2192 5027 6039 2192
Size: 2835 1012 3120
Fragments arranged by size:
3120 2835 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6967
BstBI TT'CG_AA
Cuts at: 3520 5771 3520
Size: 2251 4716
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6967
BstXI CCAn_nnnn'nTGG
Cuts at: 4039 4039
Size: 6967
BstYI r'GATC_y
Cuts at: 1972 2280 4297 4664 5049 5066 6518 6604 6616
Size: 308 2017 367 385 17 1452 86 12
Cuts at: 6616 6962 1972
Size: 346 1977
Fragments arranged by size:
2017 1977 1452 385 367 346 308 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 4243 4243
Size: 6967
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6415
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6228
Fragments arranged by size:
6228 683 56
BtsI GCAGTG_nn'
Cuts at: 2010 2049 2777 3334 4483 5277 5297 2010
Size: 39 728 557 1149 794 20 3680
Fragments arranged by size:
3680 1149 794 728 557 39 20
ClaI AT'CG_AT
Cuts at: 1554 3154 1554
Size: 1600 5367
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3484 3519 3568 3603 3904 3939 4072
Size: 35 2920 35 49 35 301 35 133
Cuts at: 4072 4107 529
Size: 35 3389
Fragments arranged by size:
3389 2920 301 133 49 35 35 35
35 35
DraI TTT'AAA
Cuts at: 2869 3135 4271 2869
Size: 266 1136 5565
Fragments arranged by size:
5565 1136 266
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5974 809
Size: 507 4658 1802
Fragments arranged by size:
4658 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3202 4414 5303
Size: 54 1425 50 275 18 1372 1212 889
Cuts at: 5303 6932 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1372 1212 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4414 1537
Size: 275 2602 4090
Fragments arranged by size:
4090 2602 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4897 1353
Size: 3544 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5554 5928 6074 387
Size: 1064 138 3965 374 146 1280
Fragments arranged by size:
3965 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2236 2332 2236
Size: 96 6871
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2158 2306 2556 2567 2595 2958
Size: 393 482 44 148 250 11 28 363
Cuts at: 2958 3243 3247 3667 3831 4015 4251 5030 5627
Size: 285 4 420 164 184 236 779 597
Cuts at: 5627 6414 6682 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 779 597 482 420 393 363
285 268 250 236 184 164 148 44
28 11 4
EcoO109I rG'GnC_Cy
Cuts at: 2682 2707 3547 2682
Size: 25 840 6102
Fragments arranged by size:
6102 840 25
EcoRI G'AATT_C
Cuts at: 3801 3969 4276 4654 3801
Size: 168 307 378 6114
Fragments arranged by size:
6114 378 307 168
FspI TGC'GCA
Cuts at: 5473 5473
Size: 6967
HaeII r_GCGC'y
Cuts at: 1678 2077 6114 1678
Size: 399 4037 2531
Fragments arranged by size:
4037 2531 399
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2461 2493 2696 2728 3531
Size: 32 135 32 889 32 203 32 803
Cuts at: 3531 3563 3682 3714 3850 3882 5608 5640 5682
Size: 32 119 32 136 32 1726 32 42
Cuts at: 5682 5714 1373
Size: 32 2626
Fragments arranged by size:
2626 1726 889 803 203 136 135 119
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2847 4311 4557 669
Size: 988 281 909 1464 246 3079
Fragments arranged by size:
3079 1464 988 909 281 246
HindIII A'AGCT_T
Cuts at: 748 3174 4098 748
Size: 2426 924 3617
Fragments arranged by size:
3617 2426 924
HpaI GTT'AAC
Cuts at: 4557 4557
Size: 6967
KasI G'GCGC_C
Cuts at: 2073 2073
Size: 6967
KpnI G_GTAC'C
Cuts at: 4292 4292
Size: 6967
MfeI C'AATT_G
Cuts at: 2180 4566 2180
Size: 2386 4581
MluI A'CGCG_T
Cuts at: 6843 6843
Size: 6967
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3668 6081 6265 1305
Size: 2363 2413 184 2007
Fragments arranged by size:
2413 2363 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3204 10
Size: 54 1425 343 1372 3773
Fragments arranged by size:
3773 1425 1372 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2412 3742 3894 3978 4925 5284 5443
Size: 648 1246 1330 152 84 947 359 159
Cuts at: 5443 518
Size: 2042
Fragments arranged by size:
2042 1330 1246 947 648 359 159 152
84
MspA1I CmG'CkG
Cuts at: 1606 2244 2350 4383 5060 6208 6453 1606
Size: 638 106 2033 677 1148 245 2120
Fragments arranged by size:
2120 2033 1148 677 638 245 106
NaeI GCC'GGC
Cuts at: 2225 2225
Size: 6967
NarI GG'CG_CC
Cuts at: 2074 2074
Size: 6967
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6415
NdeI CA'TA_TG
Cuts at: 387 2701 387
Size: 2314 4653
NgoMIV G'CCGG_C
Cuts at: 2223 2223
Size: 6967
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6967
NotI GC'GGCC_GC
Cuts at: 4414 4414
Size: 6967
NsiI A_TGCA'T
Cuts at: 2460 2460
Size: 6967
NspI r_CATG'y
Cuts at: 1132 1229 4325 5870 1132
Size: 97 3096 1545 2229
Fragments arranged by size:
3096 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2324 2564 1348
Size: 976 240 5751
Fragments arranged by size:
5751 976 240
PciI A'CATG_T
Cuts at: 5866 5866
Size: 6967
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2803 1108
Size: 1695 5272
PfoI T'CCnGG_A
Cuts at: 2283 2283
Size: 6967
PmeI GTTT'AAAC
Cuts at: 4271 4271
Size: 6967
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5036 5068 6549 6581 5036
Size: 32 1481 32 5422
Fragments arranged by size:
5422 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2707 3547 2707
Size: 840 6127
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6967
PsiI TTA'TAA
Cuts at: 4537 4537
Size: 6967
PstI C_TGCA'G
Cuts at: 830 2017 2772 4319 830
Size: 1187 755 1547 3478
Fragments arranged by size:
3478 1547 1187 755
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6967
PvuII CAG'CTG
Cuts at: 2350 2350
Size: 6967
SacI G_AGCT'C
Cuts at: 2238 2334 2238
Size: 96 6871
SalI G'TCGA_C
Cuts at: 1936 4309 1936
Size: 2373 4594
SbfI CC_TGCA'GG
Cuts at: 4319 4319
Size: 6967
ScaI AGT'ACT
Cuts at: 5215 6927 5215
Size: 1712 5255
SfcI C'TryA_G
Cuts at: 826 1045 2013 2715 2768 3346 4315 5450 6131
Size: 219 968 702 53 578 969 1135 681
Cuts at: 6131 6322 826
Size: 191 1471
Fragments arranged by size:
1471 1135 969 968 702 681 578 219
191 53
SfoI GGC'GCC
Cuts at: 2075 2075
Size: 6967
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6967
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6967
SmaI CCC'GGG
Cuts at: 1872 4294 1872
Size: 2422 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2300 2628 5071 5972 6234 820
Size: 197 926 357 328 2443 901 262 1553
Fragments arranged by size:
2443 1553 926 901 357 328 262 197
SnaBI TAC'GTA
Cuts at: 493 4758 493
Size: 4265 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6967
SphI G_CATG'C
Cuts at: 1132 4325 1132
Size: 3193 3774
SspI AAT'ATT
Cuts at: 5 52 4891 5
Size: 47 4839 2081
Fragments arranged by size:
4839 2081 47
StuI AGG'CCT
Cuts at: 1517 2034 3344 1517
Size: 517 1310 5140
Fragments arranged by size:
5140 1310 517
StyI C'CwwG_G
Cuts at: 513 1065 2271 2811 4406 513
Size: 552 1206 540 1595 3074
Fragments arranged by size:
3074 1595 1206 552 540
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2332 4994 5011 5164 5349 5794 325
Size: 1255 752 2662 17 153 185 445 1498
Fragments arranged by size:
2662 1498 1255 752 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2603 2665 4126
Size: 274 80 33 51 1443 626 62 1461
Cuts at: 4126 5213 6925 96
Size: 1087 1712 138
Fragments arranged by size:
1712 1461 1443 1087 626 274 138 80
62 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2562 2594 6927 6959 2562
Size: 32 4333 32 2570
Fragments arranged by size:
4333 2570 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6967
XbaI T'CTAG_A
Cuts at: 4303 4303
Size: 6967
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2198 2582 2198
Size: 384 6583
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6967
XmaI C'CCGG_G
Cuts at: 1870 4292 1870
Size: 2422 4545
XmnI GAAnn'nnTTC
Cuts at: 3260 3764 4016 5096 3260
Size: 504 252 1080 5131
Fragments arranged by size:
5131 1080 504 252
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4775 276
Size: 53 83 186 936 3241 2468
Fragments arranged by size:
3241 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BsiWI
BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI