Restriction map for
pFN21AB6244
(Circular) (Six-base) MAPSORT of: pFN21AB6244.seq Check: 398 from: 1 to: 6745
pFN21AB6244 6745 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4555 278
Size: 53 83 186 936 3019 2468
Fragments arranged by size:
3019 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2694 4088 1937
Size: 757 1394 4594
Fragments arranged by size:
4594 1394 757
Acc65I G'GTAC_C
Cuts at: 2866 4066 2866
Size: 1200 5545
AclI AA'CG_TT
Cuts at: 1563 4872 5245 1563
Size: 3309 373 3063
Fragments arranged by size:
3309 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2618 3329 4808 6192 6460 1632
Size: 986 711 1479 1384 268 1917
Fragments arranged by size:
1917 1479 1384 986 711 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6548
AflIII A'CryG_T
Cuts at: 1203 2538 5644 6621 1203
Size: 1335 3106 977 1327
Fragments arranged by size:
3106 1335 1327 977
AgeI A'CCGG_T
Cuts at: 5576 5576
Size: 6745
AhdI GACnn_n'nnGTC
Cuts at: 5474 5474
Size: 6745
AleI CACnn'nnGTG
Cuts at: 1166 2720 1166
Size: 1554 5191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2644 3548 1184
Size: 1460 904 4381
Fragments arranged by size:
4381 1460 904
AlwNI CAG_nnn'CTG
Cuts at: 3712 4488 6060 3712
Size: 776 1572 4397
Fragments arranged by size:
4397 1572 776
ApaLI G'TGCA_C
Cuts at: 4802 5958 4802
Size: 1156 5589
ApoI r'AATT_y
Cuts at: 1493 2008 2147 2759 2897 3830 4054 4280 4432
Size: 515 139 612 138 933 224 226 152
Cuts at: 4432 6541 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 933 612 515 226 224 152
139 138
AseI AT'TA_AT
Cuts at: 160 1032 5299 160
Size: 872 4267 1606
Fragments arranged by size:
4267 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6745
AvaI C'yCGr_G
Cuts at: 1870 1943 3903 3966 4070 1870
Size: 73 1960 63 104 4545
Fragments arranged by size:
4545 1960 104 73 63
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3614 3647 3614
Size: 33 6712
BamHI G'GATC_C
Cuts at: 4075 4442 4075
Size: 367 6378
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2437 2733 2866 3524 4066
Size: 325 217 627 650 296 133 658 542
Cuts at: 4066 5521 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 658 650 627 542 325 296
217 133
BanII G_rGCy'C
Cuts at: 1391 2352 3893 4064 1391
Size: 961 1541 171 4072
Fragments arranged by size:
4072 1541 961 171
BbeI G_GCGC'C
Cuts at: 2441 2441
Size: 6745
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2857 928
Size: 971 958 4816
Fragments arranged by size:
4816 971 958
BbvCI CC'TCA_GC
Cuts at: 3288 3456 3288
Size: 168 6577
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4936 4970 1903
Size: 34 2999 34 3678
Fragments arranged by size:
3678 2999 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2300 3167 3642 4637 5847 2300
Size: 867 475 995 1210 3198
Fragments arranged by size:
3198 1210 995 867 475
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6745
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2570 5356 136
Size: 107 122 71 631 605 898 2786 1525
Fragments arranged by size:
2786 1525 898 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6740 6740
Size: 6745
BlpI GC'TnA_GC
Cuts at: 2706 2750 4162 2706
Size: 44 1412 5289
Fragments arranged by size:
5289 1412 44
Bme1580I G_kGCm'C
Cuts at: 1790 2738 3527 4806 5962 1790
Size: 948 789 1279 1156 2573
Fragments arranged by size:
2573 1279 1156 948 789
BmgBI CAC'GTC
Cuts at: 1318 2539 2713 1318
Size: 1221 174 5350
Fragments arranged by size:
5350 1221 174
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2818 4476 5434
Size: 416 53 83 399 24 1401 1658 958
Cuts at: 5434 442
Size: 1753
Fragments arranged by size:
1753 1658 1401 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6745
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3757 3757
Size: 6745
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3564 3877 5405 1239
Size: 2325 313 1528 2579
Fragments arranged by size:
2579 2325 1528 313
Bpu10I CC'TnA_GC
Cuts at: 3288 3456 3774 3288
Size: 168 318 6259
Fragments arranged by size:
6259 318 168
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4870 5735 6033 4870
Size: 865 298 5582
Fragments arranged by size:
5582 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3893 5408 882
Size: 617 2394 1515 2219
Fragments arranged by size:
2394 2219 1515 617
BsaAI yAC'GTr
Cuts at: 493 1204 4536 493
Size: 711 3332 2702
Fragments arranged by size:
3332 2702 711
BsaBI GATnn'nnATC
Cuts at: 6739 6739
Size: 6745
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2438 4552 4934 5569
Size: 53 83 186 936 905 2114 382 635
Cuts at: 5569 275
Size: 1451
Fragments arranged by size:
2114 1451 936 905 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5178 5576 5850 5997 6408 5178
Size: 398 274 147 411 5515
Fragments arranged by size:
5515 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2644 2674 3818 3848 1178
Size: 30 165 30 1241 30 1144 30 4075
Fragments arranged by size:
4075 1241 1144 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2377 2652 3826 4049 1186
Size: 1191 275 1174 223 3882
Fragments arranged by size:
3882 1191 1174 275 223
BseYI C'CCAG_C
Cuts at: 2080 2459 5948 6444 2080
Size: 379 3489 496 2381
Fragments arranged by size:
3489 2381 496 379
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3598 1163
Size: 573 1862 4310
Fragments arranged by size:
4310 1862 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4195 4956 5984 1540
Size: 275 186 2194 761 1028 2301
Fragments arranged by size:
2301 2194 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2135 2561 2712 4806 4891 5962 2135
Size: 426 151 2094 85 1071 2918
Fragments arranged by size:
2918 2094 1071 426 151 85
BsmI GAATG_Cn'
Cuts at: 4256 4349 4256
Size: 93 6652
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2352 2561 2712 2738 3527 3893
Size: 399 345 217 209 151 26 789 366
Cuts at: 3893 4064 4806 4891 5962 1391
Size: 171 742 85 1071 2174
Fragments arranged by size:
2174 1071 789 742 399 366 345 217
209 171 151 85 26
BspEI T'CCGG_A
Cuts at: 6408 6408
Size: 6745
BspHI T'CATG_A
Cuts at: 4634 6364 4634
Size: 1730 5015
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4100 844
Size: 3256 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4632 1394
Size: 429 2809 3507
Fragments arranged by size:
3507 2809 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5240 5414 65
Size: 1183 3992 174 1396
Fragments arranged by size:
3992 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3816 5389 5576 1700
Size: 110 2006 1573 187 2869
Fragments arranged by size:
2869 2006 1573 187 110
BsrGI T'GTAC_A
Cuts at: 96 2913 96
Size: 2817 3928
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6745
BssSI C'ACGA_G
Cuts at: 4805 5817 4805
Size: 1012 5733
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6745
BstBI TT'CG_AA
Cuts at: 5549 5549
Size: 6745
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6745
BstYI r'GATC_y
Cuts at: 1972 2836 3033 3185 4075 4442 4827 4844 6296
Size: 864 197 152 890 367 385 17 1452
Cuts at: 6296 6382 6394 6740 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 890 864 385 367 346 197
152 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2664 2664
Size: 6745
BtgI C'CryG_G
Cuts at: 513 1065 3744 513
Size: 552 2679 3514
Fragments arranged by size:
3514 2679 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6006
Fragments arranged by size:
6006 683 56
BtsI GCAGTG_nn'
Cuts at: 3610 3874 4261 5055 5075 3610
Size: 264 387 794 20 5280
Fragments arranged by size:
5280 794 387 264 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6745
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6710
DraI TTT'AAA
Cuts at: 4049 4049
Size: 6745
DraIII CAC_nnn'GTG
Cuts at: 2027 2027
Size: 6745
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5752 809
Size: 507 4436 1802
Fragments arranged by size:
4436 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2327 2571 4192
Size: 54 1425 50 275 18 497 244 1621
Cuts at: 4192 5081 6710 8
Size: 889 1629 43
Fragments arranged by size:
1629 1621 1425 889 497 275 244 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4192 1537
Size: 275 2380 4090
Fragments arranged by size:
4090 2380 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3046 4675 1353
Size: 1693 1629 3423
Fragments arranged by size:
3423 1693 1629
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3080 3932 5332 5706 5852 387
Size: 1064 138 1491 852 1400 374 146 1280
Fragments arranged by size:
1491 1400 1280 1064 852 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2618 3329 3564 3877 4808 5405 6192
Size: 393 986 711 235 313 931 597 787
Cuts at: 6192 6460 1239
Size: 268 1524
Fragments arranged by size:
1524 986 931 787 711 597 393 313
268 235
EcoRI G'AATT_C
Cuts at: 2147 2759 2897 3830 4054 4432 2147
Size: 612 138 933 224 378 4460
Fragments arranged by size:
4460 933 612 378 224 138
FspI TGC'GCA
Cuts at: 5251 5251
Size: 6745
HaeII r_GCGC'y
Cuts at: 1678 2441 3133 5892 1678
Size: 763 692 2759 2531
Fragments arranged by size:
2759 2531 763 692
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2228 2260 2465 2497 2644
Size: 32 135 32 656 32 205 32 147
Cuts at: 2644 2661 2676 2693 2767 2799 3777 3809 3924
Size: 17 15 17 74 32 978 32 115
Cuts at: 3924 3956 5386 5418 5460 5492 1373
Size: 32 1430 32 42 32 2626
Fragments arranged by size:
2626 1430 978 656 205 147 135 115
74 42 32 32 32 32 32 32
32 32 32 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 4089 4335 669
Size: 988 281 2151 246 3079
Fragments arranged by size:
3079 2151 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6745
HpaI GTT'AAC
Cuts at: 4335 4335
Size: 6745
KasI G'GCGC_C
Cuts at: 2437 2437
Size: 6745
KpnI G_GTAC'C
Cuts at: 2870 4070 2870
Size: 1200 5545
MfeI C'AATT_G
Cuts at: 3297 4344 3297
Size: 1047 5698
MluI A'CGCG_T
Cuts at: 6621 6621
Size: 6745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2529 2923 5859 6043 1305
Size: 1224 394 2936 184 2007
Fragments arranged by size:
2936 2007 1224 394 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2329 10
Size: 54 1425 343 497 4426
Fragments arranged by size:
4426 1425 497 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2720 3515 3530 3536 4703 5062 5221
Size: 648 1554 795 15 6 1167 359 159
Cuts at: 5221 518
Size: 2042
Fragments arranged by size:
2042 1554 1167 795 648 359 159 15
6
MspA1I CmG'CkG
Cuts at: 1606 4161 4838 5986 6231 1606
Size: 2555 677 1148 245 2120
Fragments arranged by size:
2555 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2438 2438
Size: 6745
NcoI C'CATG_G
Cuts at: 513 1065 3744 513
Size: 552 2679 3514
Fragments arranged by size:
3514 2679 552
NdeI CA'TA_TG
Cuts at: 387 3379 387
Size: 2992 3753
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6745
NotI GC'GGCC_GC
Cuts at: 4192 4192
Size: 6745
NspI r_CATG'y
Cuts at: 1132 1229 2299 2631 3535 3791 4103 5648 1132
Size: 97 1070 332 904 256 312 1545 2229
Fragments arranged by size:
2229 1545 1070 904 332 312 256 97
PasI CC'CwG_GG
Cuts at: 1348 3706 1348
Size: 2358 4387
PciI A'CATG_T
Cuts at: 5644 5644
Size: 6745
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3542 1108
Size: 2434 4311
PfoI T'CCnGG_A
Cuts at: 3807 3807
Size: 6745
PmeI GTTT'AAAC
Cuts at: 4049 4049
Size: 6745
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2372 2404 4814 4846 6327 6359 2372
Size: 32 2410 32 1481 32 2758
Fragments arranged by size:
2758 2410 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6745
PsiI TTA'TAA
Cuts at: 4315 4315
Size: 6745
PstI C_TGCA'G
Cuts at: 830 4097 830
Size: 3267 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6745
SalI G'TCGA_C
Cuts at: 1936 4087 1936
Size: 2151 4594
SbfI CC_TGCA'GG
Cuts at: 4097 4097
Size: 6745
ScaI AGT'ACT
Cuts at: 2042 4993 6705 2042
Size: 2951 1712 2082
Fragments arranged by size:
2951 2082 1712
SfcI C'TryA_G
Cuts at: 826 1045 2036 3876 4093 5228 5909 6100 826
Size: 219 991 1840 217 1135 681 191 1471
Fragments arranged by size:
1840 1471 1135 991 681 219 217 191
SfoI GGC'GCC
Cuts at: 2439 2439
Size: 6745
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6745
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6745
SmaI CCC'GGG
Cuts at: 1872 3905 4072 1872
Size: 2033 167 4545
Fragments arranged by size:
4545 2033 167
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4849 5750 6012 820
Size: 197 926 2906 901 262 1553
Fragments arranged by size:
2906 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4536 493
Size: 4043 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6745
SphI G_CATG'C
Cuts at: 1132 2299 4103 1132
Size: 1167 1804 3774
Fragments arranged by size:
3774 1804 1167
SspI AAT'ATT
Cuts at: 5 52 4669 5
Size: 47 4617 2081
Fragments arranged by size:
4617 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6745
StyI C'CwwG_G
Cuts at: 513 1065 3744 4184 513
Size: 552 2679 440 3074
Fragments arranged by size:
3074 2679 552 440
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3542 3584 4772 4789 4942 5127 5572
Size: 1255 1962 42 1188 17 153 185 445
Cuts at: 5572 325
Size: 1498
Fragments arranged by size:
1962 1498 1255 1188 445 185 153 42
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2040 2334 2913
Size: 274 80 33 51 1443 63 294 579
Cuts at: 2913 3104 4991 6703 96
Size: 191 1887 1712 138
Fragments arranged by size:
1887 1712 1443 579 294 274 191 138
80 63 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2481 2513 3141 3173 3818 3850 6705 6737 2481
Size: 32 628 32 645 32 2855 32 2489
Fragments arranged by size:
2855 2489 645 628 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6745
XbaI T'CTAG_A
Cuts at: 4081 4081
Size: 6745
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3751 3751
Size: 6745
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6745
XmaI C'CCGG_G
Cuts at: 1870 3903 4070 1870
Size: 2033 167 4545
Fragments arranged by size:
4545 2033 167
XmnI GAAnn'nnTTC
Cuts at: 3983 4874 3983
Size: 891 5854
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4553 276
Size: 53 83 186 936 3019 2468
Fragments arranged by size:
3019 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BfrBI BsiWI
BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI