Restriction map for
pFN21AB6243
(Circular) (Six-base) MAPSORT of: pFN21AB6243.seq Check: 4468 from: 1 to: 6964
pFN21AB6243 6964 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4774 278
Size: 53 83 186 936 3238 2468
Fragments arranged by size:
3238 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4307 1937
Size: 2370 4594
Acc65I G'GTAC_C
Cuts at: 2421 4285 2421
Size: 1864 5100
AclI AA'CG_TT
Cuts at: 1563 2687 5091 5464 1563
Size: 1124 2404 373 3063
Fragments arranged by size:
3063 2404 1124 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2724 4026 4101 5027 6411 6679 1632
Size: 1092 1302 75 926 1384 268 1917
Fragments arranged by size:
1917 1384 1302 1092 926 268 75
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6767
AflIII A'CryG_T
Cuts at: 1203 5863 6840 1203
Size: 4660 977 1327
Fragments arranged by size:
4660 1327 977
AgeI A'CCGG_T
Cuts at: 5795 5795
Size: 6964
AhdI GACnn_n'nnGTC
Cuts at: 4189 5693 4189
Size: 1504 5460
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6964
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6964
AlwNI CAG_nnn'CTG
Cuts at: 3047 3610 4049 4180 4707 6279 3047
Size: 563 439 131 527 1572 3732
Fragments arranged by size:
3732 1572 563 527 439 131
ApaI G_GGCC'C
Cuts at: 4011 4011
Size: 6964
ApaLI G'TGCA_C
Cuts at: 2582 5021 6177 2582
Size: 2439 1156 3369
Fragments arranged by size:
3369 2439 1156
ApoI r'AATT_y
Cuts at: 1493 3061 3133 3241 3768 4273 4499 4651 6760
Size: 1568 72 108 527 505 226 152 2109
Cuts at: 6760 1493
Size: 1697
Fragments arranged by size:
2109 1697 1568 527 505 226 152 108
72
AseI AT'TA_AT
Cuts at: 160 1032 5518 160
Size: 872 4486 1606
Fragments arranged by size:
4486 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6964
AvaI C'yCGr_G
Cuts at: 1870 1943 4055 4289 1870
Size: 73 2112 234 4545
Fragments arranged by size:
4545 2112 234 73
AvrII C'CTAG_G
Cuts at: 3349 3349
Size: 6964
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2413 2446 3798 3831 2413
Size: 33 1352 33 5546
Fragments arranged by size:
5546 1352 33 33
BamHI G'GATC_C
Cuts at: 2568 4294 4661 2568
Size: 1726 367 4871
Fragments arranged by size:
4871 1726 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2110 2421 3475 3620 3737
Size: 325 217 627 323 311 1054 145 117
Cuts at: 3737 4285 5740 618
Size: 548 1455 1842
Fragments arranged by size:
1842 1455 1054 627 548 325 323 311
217 145 117
BanII G_rGCy'C
Cuts at: 1391 3588 3842 3992 4011 4283 1391
Size: 2197 254 150 19 272 4072
Fragments arranged by size:
4072 2197 272 254 150 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2495 928
Size: 971 596 5397
Fragments arranged by size:
5397 971 596
BbvCI CC'TCA_GC
Cuts at: 3089 3316 3089
Size: 227 6737
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5155 5189 1903
Size: 34 3218 34 3678
Fragments arranged by size:
3678 3218 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2989 4856 6066 2989
Size: 1867 1210 3887
Fragments arranged by size:
3887 1867 1210
BclI T'GATC_A
Cuts at: 1548 3873 1548
Size: 2325 4639
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5575 136
Size: 107 122 71 631 605 3903 1525
Fragments arranged by size:
3903 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6959 6959
Size: 6964
BlpI GC'TnA_GC
Cuts at: 4381 4381
Size: 6964
Bme1580I G_kGCm'C
Cuts at: 1790 2586 4011 5025 6181 1790
Size: 796 1425 1014 1156 2573
Fragments arranged by size:
2573 1425 1156 1014 796
BmgBI CAC'GTC
Cuts at: 1318 2614 1318
Size: 1296 5668
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2874 4695 5653
Size: 416 53 83 399 24 1457 1821 958
Cuts at: 5653 442
Size: 1753
Fragments arranged by size:
1821 1753 1457 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6964
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3390 3963 4157 5624 1239
Size: 2151 573 194 1467 2579
Fragments arranged by size:
2579 2151 1467 573 194
Bpu10I CC'TnA_GC
Cuts at: 2084 3089 3316 2084
Size: 1005 227 5732
Fragments arranged by size:
5732 1005 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3046 5089 5954 6252 3046
Size: 2043 865 298 3758
Fragments arranged by size:
3758 2043 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5627 882
Size: 617 4128 2219
Fragments arranged by size:
4128 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2575 4755 493
Size: 711 1371 2180 2702
Fragments arranged by size:
2702 2180 1371 711
BsaBI GATnn'nnATC
Cuts at: 3000 3878 6958 3000
Size: 878 3080 3006
Fragments arranged by size:
3080 3006 878
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4771 5153 5788 275
Size: 53 83 186 936 3238 382 635 1451
Fragments arranged by size:
3238 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2392 5397 5795 6069 6216 6627 2392
Size: 3005 398 274 147 411 2729
Fragments arranged by size:
3005 2729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3652 3682 3733 3763 1178
Size: 30 165 30 2249 30 51 30 4379
Fragments arranged by size:
4379 2249 165 51 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2514 3321 3955 4072 4244 4247 1186
Size: 1328 807 634 117 172 3 3903
Fragments arranged by size:
3903 1328 807 634 172 117 3
BseYI C'CCAG_C
Cuts at: 3297 3363 3512 4010 4024 6167 6663 3297
Size: 66 149 498 14 2143 496 3598
Fragments arranged by size:
3598 2143 498 496 149 66 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2306 2636 1163
Size: 573 570 330 5491
Fragments arranged by size:
5491 573 570 330
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4414 5175 6203 1540
Size: 275 186 2413 761 1028 2301
Fragments arranged by size:
2413 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2586 3842 5025 5110 6181 2586
Size: 1256 1183 85 1071 3369
Fragments arranged by size:
3369 1256 1183 1071 85
BsmI GAATG_Cn'
Cuts at: 2633 4475 4568 2633
Size: 1842 93 5029
Fragments arranged by size:
5029 1842 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2586 3588 3842 3992 4011 4283 5025
Size: 399 796 1002 254 150 19 272 742
Cuts at: 5025 5110 6181 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1002 796 742 399 272 254
150 85 19
BspEI T'CCGG_A
Cuts at: 2392 6627 2392
Size: 4235 2729
BspHI T'CATG_A
Cuts at: 2260 2941 4853 6583 2260
Size: 681 1912 1730 2641
Fragments arranged by size:
2641 1912 1730 681
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2481 3319 4319 844
Size: 1637 838 1000 3489
Fragments arranged by size:
3489 1637 1000 838
BsrBI CCG'CTC
Cuts at: 1394 1823 3937 4851 1394
Size: 429 2114 914 3507
Fragments arranged by size:
3507 2114 914 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2274 5459 5633 65
Size: 1183 1026 3185 174 1396
Fragments arranged by size:
3185 1396 1183 1026 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5608 5795 1700
Size: 110 3798 187 2869
Fragments arranged by size:
3798 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2646 96
Size: 2550 4414
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6964
BssSI C'ACGA_G
Cuts at: 5024 6036 5024
Size: 1012 5952
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6964
BstBI TT'CG_AA
Cuts at: 2755 5768 2755
Size: 3013 3951
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6964
BstXI CCAn_nnnn'nTGG
Cuts at: 3854 3854
Size: 6964
BstYI r'GATC_y
Cuts at: 1972 2568 3160 4294 4661 5046 5063 6515 6601
Size: 596 592 1134 367 385 17 1452 86
Cuts at: 6601 6613 6959 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1134 596 592 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2564 2564
Size: 6964
BtgI C'CryG_G
Cuts at: 513 1065 2004 3597 4146 513
Size: 552 939 1593 549 3331
Fragments arranged by size:
3331 1593 939 552 549
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6225
Fragments arranged by size:
6225 683 56
BtsI GCAGTG_nn'
Cuts at: 3543 3807 4480 5274 5294 3543
Size: 264 673 794 20 5213
Fragments arranged by size:
5213 794 673 264 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6964
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6929
DraI TTT'AAA
Cuts at: 4268 4268
Size: 6964
DraIII CAC_nnn'GTG
Cuts at: 2735 2735
Size: 6964
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5971 809
Size: 507 4655 1802
Fragments arranged by size:
4655 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4411 5300 6929
Size: 54 1425 50 275 18 2581 889 1629
Cuts at: 6929 8
Size: 43
Fragments arranged by size:
2581 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4411 1537
Size: 275 2599 4090
Fragments arranged by size:
4090 2599 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2232 3264 3885 4045 4894 1353
Size: 879 1032 621 160 849 3423
Fragments arranged by size:
3423 1032 879 849 621 160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3216 3324 5551 5925 6071 387
Size: 1064 138 1627 108 2227 374 146 1280
Fragments arranged by size:
2227 1627 1280 1064 374 146 138 108
EcoICRI GAG'CTC
Cuts at: 3840 3840
Size: 6964
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2724 3390 3963 4026 4101 4157 5027
Size: 393 1092 666 573 63 75 56 870
Cuts at: 5027 5624 6411 6679 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1092 870 787 666 597 573 393
268 75 63 56
EcoO109I rG'GnC_Cy
Cuts at: 2164 3085 3353 2164
Size: 921 268 5775
Fragments arranged by size:
5775 921 268
EcoRI G'AATT_C
Cuts at: 3133 4273 4651 3133
Size: 1140 378 5446
Fragments arranged by size:
5446 1140 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2564 3476 2564
Size: 912 6052
FspI TGC'GCA
Cuts at: 5470 5470
Size: 6964
HaeII r_GCGC'y
Cuts at: 1678 6111 1678
Size: 4433 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3194 3226 3926 3958 4151
Size: 32 135 32 1622 32 700 32 193
Cuts at: 4151 4175 4183 4207 5605 5637 5679 5711 1373
Size: 24 8 24 1398 32 42 32 2626
Fragments arranged by size:
2626 1622 1398 700 193 135 42 32
32 32 32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 4308 4554 669
Size: 988 281 2370 246 3079
Fragments arranged by size:
3079 2370 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6964
HpaI GTT'AAC
Cuts at: 4554 4554
Size: 6964
KpnI G_GTAC'C
Cuts at: 2425 4289 2425
Size: 1864 5100
MfeI C'AATT_G
Cuts at: 4563 4563
Size: 6964
MluI A'CGCG_T
Cuts at: 6840 6840
Size: 6964
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2364 6078 6262 1305
Size: 1059 3714 184 2007
Fragments arranged by size:
3714 2007 1059 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5142
Fragments arranged by size:
5142 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2342 2605 2782 2856 3901 4151 4922
Size: 648 1176 263 177 74 1045 250 771
Cuts at: 4922 5281 5440 518
Size: 359 159 2042
Fragments arranged by size:
2042 1176 1045 771 648 359 263 250
177 159 74
MspA1I CmG'CkG
Cuts at: 1606 3533 3548 4380 5057 6205 6450 1606
Size: 1927 15 832 677 1148 245 2120
Fragments arranged by size:
2120 1927 1148 832 677 245 15
NcoI C'CATG_G
Cuts at: 513 1065 2004 3597 4146 513
Size: 552 939 1593 549 3331
Fragments arranged by size:
3331 1593 939 552 549
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6964
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6964
NotI GC'GGCC_GC
Cuts at: 4411 4411
Size: 6964
NspI r_CATG'y
Cuts at: 1132 1229 3900 3918 4322 5867 1132
Size: 97 2671 18 404 1545 2229
Fragments arranged by size:
2671 2229 1545 404 97 18
PasI CC'CwG_GG
Cuts at: 1348 2275 3076 3616 3833 1348
Size: 927 801 540 217 4479
Fragments arranged by size:
4479 927 801 540 217
PciI A'CATG_T
Cuts at: 5863 5863
Size: 6964
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4180 1108
Size: 3072 3892
PmeI GTTT'AAAC
Cuts at: 4268 4268
Size: 6964
PmlI CAC'GTG
Cuts at: 2575 2575
Size: 6964
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5033 5065 6546 6578 5033
Size: 32 1481 32 5419
Fragments arranged by size:
5419 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2164 3085 3353 2164
Size: 921 268 5775
Fragments arranged by size:
5775 921 268
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6964
PsiI TTA'TAA
Cuts at: 4534 4534
Size: 6964
PspOMI G'GGCC_C
Cuts at: 4007 4007
Size: 6964
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3702 3734 3702
Size: 32 6932
PstI C_TGCA'G
Cuts at: 830 2382 2714 3690 3814 4020 4224 4316 830
Size: 1552 332 976 124 206 204 92 3478
Fragments arranged by size:
3478 1552 976 332 206 204 124 92
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6964
PvuII CAG'CTG
Cuts at: 3548 3548
Size: 6964
SacI G_AGCT'C
Cuts at: 3842 3842
Size: 6964
SalI G'TCGA_C
Cuts at: 1936 4306 1936
Size: 2370 4594
SbfI CC_TGCA'GG
Cuts at: 4316 4316
Size: 6964
ScaI AGT'ACT
Cuts at: 3577 5212 6924 3577
Size: 1635 1712 3617
Fragments arranged by size:
3617 1712 1635
SfcI C'TryA_G
Cuts at: 826 1045 2378 2455 2710 3686 3810 4016 4220
Size: 219 1333 77 255 976 124 206 204
Cuts at: 4220 4312 5447 6128 6319 826
Size: 92 1135 681 191 1471
Fragments arranged by size:
1471 1333 1135 976 681 255 219 206
204 191 124 92 77
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6964
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6964
SmaI CCC'GGG
Cuts at: 1872 4057 4291 1872
Size: 2185 234 4545
Fragments arranged by size:
4545 2185 234
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3025 5068 5969 6231 820
Size: 197 926 1082 2043 901 262 1553
Fragments arranged by size:
2043 1553 1082 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4755 493
Size: 4262 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6964
SphI G_CATG'C
Cuts at: 1132 3918 4322 1132
Size: 2786 404 3774
Fragments arranged by size:
3774 2786 404
SspI AAT'ATT
Cuts at: 5 52 2356 4888 5
Size: 47 2304 2532 2081
Fragments arranged by size:
2532 2304 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6964
StyI C'CwwG_G
Cuts at: 513 1065 2004 3349 3597 4146 4260 4403 513
Size: 552 939 1345 248 549 114 143 3074
Fragments arranged by size:
3074 1345 939 552 549 248 143 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2356 3608 4991 5008 5161 5346 5791
Size: 1255 776 1252 1383 17 153 185 445
Cuts at: 5791 325
Size: 1498
Fragments arranged by size:
1498 1383 1255 1252 776 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2505 2646 3268
Size: 274 80 33 51 1443 528 141 622
Cuts at: 3268 3575 5210 6922 96
Size: 307 1635 1712 138
Fragments arranged by size:
1712 1635 1443 622 528 307 274 141
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3652 3684 3733 3765 6924 6956 3652
Size: 32 49 32 3159 32 3660
Fragments arranged by size:
3660 3159 49 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6964
XbaI T'CTAG_A
Cuts at: 4300 4300
Size: 6964
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3700 4001 3700
Size: 301 6663
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6964
XmaI C'CCGG_G
Cuts at: 1870 4055 4289 1870
Size: 2185 234 4545
Fragments arranged by size:
4545 2185 234
XmnI GAAnn'nnTTC
Cuts at: 2633 2900 3768 5093 2633
Size: 267 868 1325 4504
Fragments arranged by size:
4504 1325 868 267
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4772 276
Size: 53 83 186 936 3238 2468
Fragments arranged by size:
3238 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PspXI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI