Restriction map for
pFN21AB6238
(Circular) (Six-base) MAPSORT of: pFN21AB6238.seq Check: 3026 from: 1 to: 6736
pFN21AB6238 6736 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2391 4546 278
Size: 53 83 186 936 855 2155 2468
Fragments arranged by size:
2468 2155 936 855 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4079 1937
Size: 2142 4594
Acc65I G'GTAC_C
Cuts at: 4057 4057
Size: 6736
AclI AA'CG_TT
Cuts at: 1563 4863 5236 1563
Size: 3300 373 3063
Fragments arranged by size:
3300 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2045 2502 2519 2558 2648 3545 4799 6183
Size: 413 457 17 39 90 897 1254 1384
Cuts at: 6183 6451 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1254 897 457 413 268 90
39 17
AfeI AGC'GCT
Cuts at: 2868 2868
Size: 6736
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6539
AflIII A'CryG_T
Cuts at: 1203 5635 6612 1203
Size: 4432 977 1327
Fragments arranged by size:
4432 1327 977
AgeI A'CCGG_T
Cuts at: 5567 5567
Size: 6736
AhdI GACnn_n'nnGTC
Cuts at: 5465 5465
Size: 6736
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6736
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2057 2757 1184
Size: 873 700 5163
Fragments arranged by size:
5163 873 700
AlwNI CAG_nnn'CTG
Cuts at: 2770 3562 3664 4479 6051 2770
Size: 792 102 815 1572 3455
Fragments arranged by size:
3455 1572 815 792 102
ApaI G_GGCC'C
Cuts at: 2617 3375 3409 2617
Size: 758 34 5944
Fragments arranged by size:
5944 758 34
ApaLI G'TGCA_C
Cuts at: 4793 5949 4793
Size: 1156 5580
ApoI r'AATT_y
Cuts at: 1493 3356 4045 4271 4423 6532 1493
Size: 1863 689 226 152 2109 1697
Fragments arranged by size:
2109 1863 1697 689 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5290 160
Size: 872 4258 1606
Fragments arranged by size:
4258 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
AvaI C'yCGr_G
Cuts at: 1870 1943 2373 2441 3402 4061 1870
Size: 73 430 68 961 659 4545
Fragments arranged by size:
4545 961 659 430 73 68
BamHI G'GATC_C
Cuts at: 3210 4066 4433 3210
Size: 856 367 5513
Fragments arranged by size:
5513 856 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2377 2593 4057 5512 618
Size: 325 217 627 590 216 1464 1455 1842
Fragments arranged by size:
1842 1464 1455 627 590 325 217 216
BanII G_rGCy'C
Cuts at: 1391 2617 2705 3375 3409 4055 1391
Size: 1226 88 670 34 646 4072
Fragments arranged by size:
4072 1226 670 646 88 34
BbeI G_GCGC'C
Cuts at: 2597 2597
Size: 6736
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2532 3110 928
Size: 971 633 578 4554
Fragments arranged by size:
4554 971 633 578
BbvCI CC'TCA_GC
Cuts at: 2010 3671 4023 2010
Size: 1661 352 4723
Fragments arranged by size:
4723 1661 352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4927 4961 1903
Size: 34 2990 34 3678
Fragments arranged by size:
3678 2990 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4628 5838 4628
Size: 1210 5526
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6736
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2720 3350 5347
Size: 107 122 71 631 605 1048 630 1997
Cuts at: 5347 136
Size: 1525
Fragments arranged by size:
1997 1525 1048 631 630 605 122 107
71
BglII A'GATC_T
Cuts at: 3021 6731 3021
Size: 3710 3026
BlpI GC'TnA_GC
Cuts at: 2030 2411 3147 3239 3426 4153 2030
Size: 381 736 92 187 727 4613
Fragments arranged by size:
4613 736 727 381 187 92
Bme1580I G_kGCm'C
Cuts at: 1790 2382 2617 2989 3375 3409 3931 4797 5953
Size: 592 235 372 386 34 522 866 1156
Cuts at: 5953 1790
Size: 2573
Fragments arranged by size:
2573 1156 866 592 522 386 372 235
34
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6736
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2992 4467 5425
Size: 416 53 83 399 24 1575 1475 958
Cuts at: 5425 442
Size: 1753
Fragments arranged by size:
1753 1575 1475 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2627 3622 3867 3954 3960 5396 1239
Size: 1388 995 245 87 6 1436 2579
Fragments arranged by size:
2579 1436 1388 995 245 87 6
Bpu10I CC'TnA_GC
Cuts at: 2010 3671 3691 4023 2010
Size: 1661 20 332 4723
Fragments arranged by size:
4723 1661 332 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4861 5726 6024 4861
Size: 865 298 5573
Fragments arranged by size:
5573 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2099 3763 3835 5399 882
Size: 617 600 1664 72 1564 2219
Fragments arranged by size:
2219 1664 1564 617 600 72
BsaAI yAC'GTr
Cuts at: 493 1204 4527 493
Size: 711 3323 2702
Fragments arranged by size:
3323 2702 711
BsaBI GATnn'nnATC
Cuts at: 6730 6730
Size: 6736
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2388 2594 3107 3342
Size: 53 83 186 936 855 206 513 235
Cuts at: 3342 4543 4925 5560 275
Size: 1201 382 635 1451
Fragments arranged by size:
1451 1201 936 855 635 513 382 235
206 186 83 53
BsaWI w'CCGG_w
Cuts at: 3252 4033 5169 5567 5841 5988 6399 3252
Size: 781 1136 398 274 147 411 3589
Fragments arranged by size:
3589 1136 781 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4001 4031 1178
Size: 30 165 30 2598 30 3883
Fragments arranged by size:
3883 2598 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2117 2342 3368 3594 1186
Size: 841 90 225 1026 226 4328
Fragments arranged by size:
4328 1026 841 226 225 90
BseYI C'CCAG_C
Cuts at: 2455 3090 3151 3654 3731 3782 3953 4002 5939
Size: 635 61 503 77 51 171 49 1937
Cuts at: 5939 6435 2455
Size: 496 2756
Fragments arranged by size:
2756 1937 635 503 496 171 77 61
51 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3293 3555 3758 1163
Size: 573 1557 262 203 4141
Fragments arranged by size:
4141 1557 573 262 203
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4186 4947 5975 1540
Size: 275 186 2185 761 1028 2301
Fragments arranged by size:
2301 2185 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3301 4797 4882 5953 3301
Size: 1496 85 1071 4084
Fragments arranged by size:
4084 1496 1071 85
BsmI GAATG_Cn'
Cuts at: 4247 4340 4247
Size: 93 6643
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2382 2617 2705 2989 3301 3375 3409
Size: 399 592 235 88 284 312 74 34
Cuts at: 3409 3931 4055 4797 4882 5953 1391
Size: 522 124 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 592 522 399 312 284
235 124 88 85 74 34
BspEI T'CCGG_A
Cuts at: 6399 6399
Size: 6736
BspHI T'CATG_A
Cuts at: 4625 6355 4625
Size: 1730 5006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2117 2942 3421 3649 4091 844
Size: 1273 825 479 228 442 3489
Fragments arranged by size:
3489 1273 825 479 442 228
BsrBI CCG'CTC
Cuts at: 1394 1823 3208 3578 4623 1394
Size: 429 1385 370 1045 3507
Fragments arranged by size:
3507 1385 1045 429 370
BsrDI GCAATG_nn'
Cuts at: 65 1248 2739 5231 5405 65
Size: 1183 1491 2492 174 1396
Fragments arranged by size:
2492 1491 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5380 5567 1700
Size: 110 3570 187 2869
Fragments arranged by size:
3570 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6736
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6736
BssSI C'ACGA_G
Cuts at: 2645 3573 4796 5808 2645
Size: 928 1223 1012 3573
Fragments arranged by size:
3573 1223 1012 928
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2162 794
Size: 1368 5368
BstBI TT'CG_AA
Cuts at: 5540 5540
Size: 6736
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6736
BstXI CCAn_nnnn'nTGG
Cuts at: 2065 2065
Size: 6736
BstYI r'GATC_y
Cuts at: 1972 3021 3210 4066 4433 4818 4835 6287 6373
Size: 1049 189 856 367 385 17 1452 86
Cuts at: 6373 6385 6731 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1049 856 385 367 346 189
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2079 2745 2774 513
Size: 552 939 75 666 29 4475
Fragments arranged by size:
4475 939 666 552 75 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2071 3118 3526 487
Size: 683 56 845 1047 408 3697
Fragments arranged by size:
3697 1047 845 683 408 56
BtsI GCAGTG_nn'
Cuts at: 3223 3786 3931 4252 5046 5066 3223
Size: 563 145 321 794 20 4893
Fragments arranged by size:
4893 794 563 321 145 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6736
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6701
DraI TTT'AAA
Cuts at: 4040 4040
Size: 6736
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3565 5743 809
Size: 507 2249 2178 1802
Fragments arranged by size:
2249 2178 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4183 5072 6701
Size: 54 1425 50 275 18 2353 889 1629
Cuts at: 6701 8
Size: 43
Fragments arranged by size:
2353 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4183 1537
Size: 275 2371 4090
Fragments arranged by size:
4090 2371 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4666 1353
Size: 3313 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2738 5323 5697 5843 387
Size: 1064 138 1149 2585 374 146 1280
Fragments arranged by size:
2585 1280 1149 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2502 2519 2558 2627 2648 3545
Size: 393 413 457 17 39 69 21 897
Cuts at: 3545 3622 3867 3954 3960 4799 5396 6183 6451
Size: 77 245 87 6 839 597 787 268
Cuts at: 6451 1239
Size: 1524
Fragments arranged by size:
1524 897 839 787 597 457 413 393
268 245 87 77 69 39 21 17
6
EcoNI CCTnn'n_nnAGG
Cuts at: 3485 3485
Size: 6736
EcoO109I rG'GnC_Cy
Cuts at: 2613 2614 3363 3372 3490 3661 3863 2613
Size: 1 749 9 118 171 202 5486
Fragments arranged by size:
5486 749 202 171 118 9 1
EcoRI G'AATT_C
Cuts at: 3356 4045 4423 3356
Size: 689 378 5669
Fragments arranged by size:
5669 689 378
FspI TGC'GCA
Cuts at: 5242 5242
Size: 6736
HaeII r_GCGC'y
Cuts at: 1678 2597 2870 3645 5883 1678
Size: 919 273 775 2238 2531
Fragments arranged by size:
2531 2238 919 775 273
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5377 5409 5451 5483 1373
Size: 32 135 32 3805 32 42 32 2626
Fragments arranged by size:
3805 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3436 4080 4326 669
Size: 988 281 1498 644 246 3079
Fragments arranged by size:
3079 1498 988 644 281 246
HindIII A'AGCT_T
Cuts at: 748 2027 748
Size: 1279 5457
HpaI GTT'AAC
Cuts at: 4326 4326
Size: 6736
KasI G'GCGC_C
Cuts at: 2593 2593
Size: 6736
KpnI G_GTAC'C
Cuts at: 4061 4061
Size: 6736
MfeI C'AATT_G
Cuts at: 4335 4335
Size: 6736
MluI A'CGCG_T
Cuts at: 6612 6612
Size: 6736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3632 5850 6034 1305
Size: 2327 2218 184 2007
Fragments arranged by size:
2327 2218 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4914
Fragments arranged by size:
4914 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2063 2180 4694 5053 5212 518
Size: 648 897 117 2514 359 159 2042
Fragments arranged by size:
2514 2042 897 648 359 159 117
MspA1I CmG'CkG
Cuts at: 1606 2116 2924 3921 4152 4829 5977 6222 1606
Size: 510 808 997 231 677 1148 245 2120
Fragments arranged by size:
2120 1148 997 808 677 510 245 231
NarI GG'CG_CC
Cuts at: 2594 2594
Size: 6736
NcoI C'CATG_G
Cuts at: 513 1065 2004 2079 513
Size: 552 939 75 5170
Fragments arranged by size:
5170 939 552 75
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6736
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6736
NotI GC'GGCC_GC
Cuts at: 4183 4183
Size: 6736
NspI r_CATG'y
Cuts at: 1132 1229 4094 5639 1132
Size: 97 2865 1545 2229
Fragments arranged by size:
2865 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6736
PciI A'CATG_T
Cuts at: 5635 5635
Size: 6736
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2255 3090 3500 3562 1108
Size: 1147 835 410 62 4282
Fragments arranged by size:
4282 1147 835 410 62
PfoI T'CCnGG_A
Cuts at: 3062 3062
Size: 6736
PmeI GTTT'AAAC
Cuts at: 4040 4040
Size: 6736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4805 4837 6318 6350 4805
Size: 32 1481 32 5191
Fragments arranged by size:
5191 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3363 3661 3363
Size: 298 6438
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6736
PsiI TTA'TAA
Cuts at: 4306 4306
Size: 6736
PspOMI G'GGCC_C
Cuts at: 2613 3371 3405 2613
Size: 758 34 5944
Fragments arranged by size:
5944 758 34
PstI C_TGCA'G
Cuts at: 830 3195 3423 4088 830
Size: 2365 228 665 3478
Fragments arranged by size:
3478 2365 665 228
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6736
PvuII CAG'CTG
Cuts at: 2116 2924 2116
Size: 808 5928
SalI G'TCGA_C
Cuts at: 1936 4078 1936
Size: 2142 4594
SanDI GG'GwC_CC
Cuts at: 3363 3363
Size: 6736
SbfI CC_TGCA'GG
Cuts at: 4088 4088
Size: 6736
ScaI AGT'ACT
Cuts at: 4984 6696 4984
Size: 1712 5024
SfcI C'TryA_G
Cuts at: 826 1045 3191 3419 4084 5219 5900 6091 826
Size: 219 2146 228 665 1135 681 191 1471
Fragments arranged by size:
2146 1471 1135 681 665 228 219 191
SfoI GGC'GCC
Cuts at: 2595 2595
Size: 6736
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6736
SmaI CCC'GGG
Cuts at: 1872 2375 4063 1872
Size: 503 1688 4545
Fragments arranged by size:
4545 1688 503
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4840 5741 6003 820
Size: 197 926 2897 901 262 1553
Fragments arranged by size:
2897 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4527 493
Size: 4034 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6736
SphI G_CATG'C
Cuts at: 1132 4094 1132
Size: 2962 3774
SspI AAT'ATT
Cuts at: 5 52 4660 5
Size: 47 4608 2081
Fragments arranged by size:
4608 2081 47
StuI AGG'CCT
Cuts at: 1517 2693 1517
Size: 1176 5560
StyI C'CwwG_G
Cuts at: 513 1065 2004 2079 3367 4175 513
Size: 552 939 75 1288 808 3074
Fragments arranged by size:
3074 1288 939 808 552 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3082 3668 4763 4780 4933 5118 5563
Size: 1255 1502 586 1095 17 153 185 445
Cuts at: 5563 325
Size: 1498
Fragments arranged by size:
1502 1498 1255 1095 586 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3819 4982 6694
Size: 274 80 33 51 1443 1842 1163 1712
Cuts at: 6694 96
Size: 138
Fragments arranged by size:
1842 1712 1443 1163 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3202 3234 3537 3569 3859 3891 6696 6728 3202
Size: 32 303 32 290 32 2805 32 3210
Fragments arranged by size:
3210 2805 303 290 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6736
XbaI T'CTAG_A
Cuts at: 2893 4072 2893
Size: 1179 5557
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2065 3091 3139 3751 2065
Size: 1026 48 612 5050
Fragments arranged by size:
5050 1026 612 48
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6736
XmaI C'CCGG_G
Cuts at: 1870 2373 4061 1870
Size: 503 1688 4545
Fragments arranged by size:
4545 1688 503
XmnI GAAnn'nnTTC
Cuts at: 4865 4865
Size: 6736
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2389 4544 276
Size: 53 83 186 936 855 2155 2468
Fragments arranged by size:
2468 2155 936 855 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SrfI
SwaI