Restriction map for
pFN21AB6236
(Circular) (Six-base) MAPSORT of: pFN21AB6236.seq Check: 4510 from: 1 to: 6733
pFN21AB6236 6733 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4543 278
Size: 53 83 186 936 3007 2468
Fragments arranged by size:
3007 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2174 4076 1937
Size: 237 1902 4594
Fragments arranged by size:
4594 1902 237
Acc65I G'GTAC_C
Cuts at: 4054 4054
Size: 6733
AclI AA'CG_TT
Cuts at: 1563 4860 5233 1563
Size: 3297 373 3063
Fragments arranged by size:
3297 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3128 3593 3847 4796 6180 6448 1632
Size: 1496 465 254 949 1384 268 1917
Fragments arranged by size:
1917 1496 1384 949 465 268 254
AfeI AGC'GCT
Cuts at: 2285 2285
Size: 6733
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6536
AflIII A'CryG_T
Cuts at: 1203 5632 6609 1203
Size: 4429 977 1327
Fragments arranged by size:
4429 1327 977
AgeI A'CCGG_T
Cuts at: 5564 5564
Size: 6733
AhdI GACnn_n'nnGTC
Cuts at: 3598 5462 3598
Size: 1864 4869
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6733
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2822 1184
Size: 1638 5095
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 6701
AlwNI CAG_nnn'CTG
Cuts at: 2249 2930 3571 4476 6048 2249
Size: 681 641 905 1572 2934
Fragments arranged by size:
2934 1572 905 681 641
ApaLI G'TGCA_C
Cuts at: 2807 3181 4790 5946 2807
Size: 374 1609 1156 3594
Fragments arranged by size:
3594 1609 1156 374
ApoI r'AATT_y
Cuts at: 1493 4010 4042 4268 4420 6529 1493
Size: 2517 32 226 152 2109 1697
Fragments arranged by size:
2517 2109 1697 226 152 32
AscI GG'CGCG_CC
Cuts at: 3929 3929
Size: 6733
AseI AT'TA_AT
Cuts at: 160 1032 5287 160
Size: 872 4255 1606
Fragments arranged by size:
4255 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6733
AvaI C'yCGr_G
Cuts at: 1870 1943 4058 1870
Size: 73 2115 4545
Fragments arranged by size:
4545 2115 73
BamHI G'GATC_C
Cuts at: 4063 4430 4063
Size: 367 6366
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2633 3533 4054 5509
Size: 325 217 627 221 625 900 521 1455
Cuts at: 5509 618
Size: 1842
Fragments arranged by size:
1842 1455 900 627 625 521 325 221
217
BanII G_rGCy'C
Cuts at: 1391 2051 2726 4052 1391
Size: 660 675 1326 4072
Fragments arranged by size:
4072 1326 675 660
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 6733
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2796 2966 928
Size: 971 897 170 4695
Fragments arranged by size:
4695 971 897 170
BbvCI CC'TCA_GC
Cuts at: 2868 2868
Size: 6733
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4924 4958 1903
Size: 34 2987 34 3678
Fragments arranged by size:
3678 2987 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2169 4625 5835 2169
Size: 2456 1210 3067
Fragments arranged by size:
3067 2456 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6733
BfrBI ATG'CAT
Cuts at: 2604 2604
Size: 6733
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3137 5344 136
Size: 107 122 71 631 605 1465 2207 1525
Fragments arranged by size:
2207 1525 1465 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3012 6728 3012
Size: 3716 3017
BlpI GC'TnA_GC
Cuts at: 2111 4150 2111
Size: 2039 4694
Bme1580I G_kGCm'C
Cuts at: 1790 2811 3185 4794 5950 1790
Size: 1021 374 1609 1156 2573
Fragments arranged by size:
2573 1609 1156 1021 374
BmgBI CAC'GTC
Cuts at: 1318 2377 3175 1318
Size: 1059 798 4876
Fragments arranged by size:
4876 1059 798
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4464 5422 442
Size: 416 53 83 399 24 3047 958 1753
Fragments arranged by size:
3047 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6733
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 6733
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2390 3761 5393 1239
Size: 1151 1371 1632 2579
Fragments arranged by size:
2579 1632 1371 1151
Bpu10I CC'TnA_GC
Cuts at: 2868 2868
Size: 6733
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3128 4858 5723 6021 3128
Size: 1730 865 298 3840
Fragments arranged by size:
3840 1730 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5396 882
Size: 617 3897 2219
Fragments arranged by size:
3897 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4524 493
Size: 711 3320 2702
Fragments arranged by size:
3320 2702 711
BsaBI GATnn'nnATC
Cuts at: 6727 6727
Size: 6733
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2184 4540 4922
Size: 53 83 186 936 476 175 2356 382
Cuts at: 4922 5557 275
Size: 635 1451
Fragments arranged by size:
2356 1451 936 635 476 382 186 175
83 53
BsaWI w'CCGG_w
Cuts at: 2963 5166 5564 5838 5985 6396 2963
Size: 2203 398 274 147 411 3300
Fragments arranged by size:
3300 2203 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2231 2261 2903 2933 1178
Size: 30 165 30 828 30 642 30 4978
Fragments arranged by size:
4978 828 642 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2252 2911 3132 1186
Size: 1066 659 221 4787
Fragments arranged by size:
4787 1066 659 221
BseYI C'CCAG_C
Cuts at: 2044 2972 3775 5936 6432 2044
Size: 928 803 2161 496 2345
Fragments arranged by size:
2345 2161 928 803 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2159 3232 1163
Size: 573 423 1073 4664
Fragments arranged by size:
4664 1073 573 423
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4183 4944 5972 1540
Size: 275 186 2182 761 1028 2301
Fragments arranged by size:
2301 2182 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2811 3185 4020 4794 4879 5950 2811
Size: 374 835 774 85 1071 3594
Fragments arranged by size:
3594 1071 835 774 374 85
BsmI GAATG_Cn'
Cuts at: 3980 4244 4337 3980
Size: 264 93 6376
Fragments arranged by size:
6376 264 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2726 2811 3185 4020 4052 4794
Size: 399 261 675 85 374 835 32 742
Cuts at: 4794 4879 5950 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 835 742 675 399 374 261
85 85 32
BspEI T'CCGG_A
Cuts at: 6396 6396
Size: 6733
BspHI T'CATG_A
Cuts at: 4622 6352 4622
Size: 1730 5003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2334 4088 844
Size: 1490 1754 3489
Fragments arranged by size:
3489 1754 1490
BsrBI CCG'CTC
Cuts at: 1394 1823 4620 1394
Size: 429 2797 3507
Fragments arranged by size:
3507 2797 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2582 5228 5402 65
Size: 1183 1334 2646 174 1396
Fragments arranged by size:
2646 1396 1334 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5377 5564 1700
Size: 110 3567 187 2869
Fragments arranged by size:
3567 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6733
BssHII G'CGCG_C
Cuts at: 1925 3929 1925
Size: 2004 4729
BssSI C'ACGA_G
Cuts at: 4793 5805 4793
Size: 1012 5721
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3033 3528 794
Size: 2239 495 3999
Fragments arranged by size:
3999 2239 495
BstBI TT'CG_AA
Cuts at: 5537 5537
Size: 6733
BstEII G'GTnAC_C
Cuts at: 1184 2254 1184
Size: 1070 5663
BstXI CCAn_nnnn'nTGG
Cuts at: 3688 3775 3688
Size: 87 6646
BstYI r'GATC_y
Cuts at: 1972 3012 3558 4063 4430 4815 4832 6284 6370
Size: 1040 546 505 367 385 17 1452 86
Cuts at: 6370 6382 6728 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1040 546 505 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2334 2334
Size: 6733
BtgI C'CryG_G
Cuts at: 513 1065 2004 2259 2437 2637 3186 3649 3966
Size: 552 939 255 178 200 549 463 317
Cuts at: 3966 513
Size: 3280
Fragments arranged by size:
3280 939 552 549 463 317 255 200
178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2008 487
Size: 683 56 782 5212
Fragments arranged by size:
5212 782 683 56
BtsI GCAGTG_nn'
Cuts at: 2407 2740 4249 5043 5063 2407
Size: 333 1509 794 20 4077
Fragments arranged by size:
4077 1509 794 333 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6733
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2482 2517 529
Size: 35 1918 35 4745
Fragments arranged by size:
4745 1918 35 35
DraI TTT'AAA
Cuts at: 4037 4037
Size: 6733
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5740 809
Size: 507 4424 1802
Fragments arranged by size:
4424 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 2640 2832
Size: 54 1425 50 275 18 447 363 192
Cuts at: 2832 3457 3634 4180 5069 6698 8
Size: 625 177 546 889 1629 43
Fragments arranged by size:
1629 1425 889 625 546 447 363 275
192 177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4180 1537
Size: 275 2368 4090
Fragments arranged by size:
4090 2368 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3677 4663 1353
Size: 2324 986 3423
Fragments arranged by size:
3423 2324 986
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5320 5694 5840 387
Size: 1064 138 3731 374 146 1280
Fragments arranged by size:
3731 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2390 3128 3593 3761 3847 4796 5393
Size: 393 758 738 465 168 86 949 597
Cuts at: 5393 6180 6448 1239
Size: 787 268 1524
Fragments arranged by size:
1524 949 787 758 738 597 465 393
268 168 86
EcoNI CCTnn'n_nnAGG
Cuts at: 3146 3146
Size: 6733
EcoO109I rG'GnC_Cy
Cuts at: 2545 3000 3064 3139 3304 3421 3568 3610 2545
Size: 455 64 75 165 117 147 42 5668
Fragments arranged by size:
5668 455 165 147 117 75 64 42
EcoRI G'AATT_C
Cuts at: 4042 4420 4042
Size: 378 6355
FspI TGC'GCA
Cuts at: 2021 2399 5239 2021
Size: 378 2840 3515
Fragments arranged by size:
3515 2840 378
HaeII r_GCGC'y
Cuts at: 1678 2012 2111 2287 5880 1678
Size: 334 99 176 3593 2531
Fragments arranged by size:
3593 2531 334 176 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2143 2156 2175 2188 2231
Size: 32 135 32 571 13 19 13 43
Cuts at: 2231 2263 2903 2935 3584 3616 5374 5406 5448
Size: 32 640 32 649 32 1758 32 42
Cuts at: 5448 5480 1373
Size: 32 2626
Fragments arranged by size:
2626 1758 649 640 571 135 43 42
32 32 32 32 32 32 32 19
13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2175 4077 4323 669
Size: 988 281 237 1902 246 3079
Fragments arranged by size:
3079 1902 988 281 246 237
HindIII A'AGCT_T
Cuts at: 748 2858 748
Size: 2110 4623
HpaI GTT'AAC
Cuts at: 4323 4323
Size: 6733
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 6733
KpnI G_GTAC'C
Cuts at: 4058 4058
Size: 6733
MfeI C'AATT_G
Cuts at: 4332 4332
Size: 6733
MluI A'CGCG_T
Cuts at: 6609 6609
Size: 6733
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3426 3831 5847 6031 1305
Size: 2121 405 2016 184 2007
Fragments arranged by size:
2121 2016 2007 405 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2834 3459 3636 10
Size: 54 1425 343 1002 625 177 3107
Fragments arranged by size:
3107 1425 1002 625 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2193 2687 2804 3654 3686 4691 5050
Size: 648 1027 494 117 850 32 1005 359
Cuts at: 5050 5209 518
Size: 159 2042
Fragments arranged by size:
2042 1027 1005 850 648 494 359 159
117 32
MspA1I CmG'CkG
Cuts at: 1606 2439 2639 3368 3711 4149 4826 5974 6219
Size: 833 200 729 343 438 677 1148 245
Cuts at: 6219 1606
Size: 2120
Fragments arranged by size:
2120 1148 833 729 677 438 343 245
200
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 6733
NcoI C'CATG_G
Cuts at: 513 1065 2004 3186 3649 3966 513
Size: 552 939 1182 463 317 3280
Fragments arranged by size:
3280 1182 939 552 463 317
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6733
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6733
NotI GC'GGCC_GC
Cuts at: 4180 4180
Size: 6733
NsiI A_TGCA'T
Cuts at: 2606 2606
Size: 6733
NspI r_CATG'y
Cuts at: 1132 1229 2692 2986 4091 5636 1132
Size: 97 1463 294 1105 1545 2229
Fragments arranged by size:
2229 1545 1463 1105 294 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6733
PciI A'CATG_T
Cuts at: 5632 5632
Size: 6733
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3825 1108
Size: 2717 4016
PfoI T'CCnGG_A
Cuts at: 2367 2900 3321 3738 4023 2367
Size: 533 421 417 285 5077
Fragments arranged by size:
5077 533 421 417 285
PmeI GTTT'AAAC
Cuts at: 4037 4037
Size: 6733
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2402 2434 4802 4834 6315 6347 2402
Size: 32 2368 32 1481 32 2788
Fragments arranged by size:
2788 2368 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3000 3064 3304 3421 3610 3000
Size: 64 240 117 189 6123
Fragments arranged by size:
6123 240 189 117 64
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6733
PsiI TTA'TAA
Cuts at: 4303 4303
Size: 6733
PstI C_TGCA'G
Cuts at: 830 2058 2331 3390 4085 830
Size: 1228 273 1059 695 3478
Fragments arranged by size:
3478 1228 1059 695 273
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6733
SacII CC_GC'GG
Cuts at: 2440 2640 2440
Size: 200 6533
SalI G'TCGA_C
Cuts at: 1936 2173 4075 1936
Size: 237 1902 4594
Fragments arranged by size:
4594 1902 237
SbfI CC_TGCA'GG
Cuts at: 2058 4085 2058
Size: 2027 4706
ScaI AGT'ACT
Cuts at: 3125 4981 6693 3125
Size: 1856 1712 3165
Fragments arranged by size:
3165 1856 1712
SfcI C'TryA_G
Cuts at: 826 1045 2054 2327 3386 3563 3892 3940 4081
Size: 219 1009 273 1059 177 329 48 141
Cuts at: 4081 5216 5897 6088 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1059 1009 681 329 273 219
191 177 141 48
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 6733
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6733
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6733
SmaI CCC'GGG
Cuts at: 1872 4060 1872
Size: 2188 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3143 4837 5738 6000 820
Size: 197 926 1200 1694 901 262 1553
Fragments arranged by size:
1694 1553 1200 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4524 493
Size: 4031 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6733
SphI G_CATG'C
Cuts at: 1132 4091 1132
Size: 2959 3774
SspI AAT'ATT
Cuts at: 5 52 4657 5
Size: 47 4605 2081
Fragments arranged by size:
4605 2081 47
StuI AGG'CCT
Cuts at: 1517 2873 1517
Size: 1356 5377
StyI C'CwwG_G
Cuts at: 513 1065 2004 2340 3186 3261 3649 3720 3729
Size: 552 939 336 846 75 388 71 9
Cuts at: 3729 3966 4172 513
Size: 237 206 3074
Fragments arranged by size:
3074 939 846 552 388 336 237 206
75 71 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2252 2903 3251 3898 4760 4777 4930
Size: 1255 672 651 348 647 862 17 153
Cuts at: 4930 5115 5560 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 862 672 651 647 445 348
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3123 3576 4979
Size: 274 80 33 51 1443 1146 453 1403
Cuts at: 4979 6691 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1403 1146 453 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6693 6725 6693
Size: 32 6701
Tth111I GACn'n_nGTC
Cuts at: 1316 2537 1316
Size: 1221 5512
XbaI T'CTAG_A
Cuts at: 4069 4069
Size: 6733
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3736 3736
Size: 6733
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6733
XmaI C'CCGG_G
Cuts at: 1870 4058 1870
Size: 2188 4545
XmnI GAAnn'nnTTC
Cuts at: 3500 4862 3500
Size: 1362 5371
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4541 276
Size: 53 83 186 936 3007 2468
Fragments arranged by size:
3007 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AvrII BaeI BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SanDI SapI SexAI SfiI SrfI SwaI