Restriction map for
pFN21AB6235
(Circular) (Six-base) MAPSORT of: pFN21AB6235.seq Check: 3990 from: 1 to: 6952
pFN21AB6235 6952 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2080 2080
Size: 6952
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3345 4762 278
Size: 53 83 186 936 1809 1417 2468
Fragments arranged by size:
2468 1809 1417 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3229 3591 3886 4295 1937
Size: 1292 362 295 409 4594
Fragments arranged by size:
4594 1292 409 362 295
Acc65I G'GTAC_C
Cuts at: 4273 4273
Size: 6952
AclI AA'CG_TT
Cuts at: 1563 5079 5452 1563
Size: 3516 373 3063
Fragments arranged by size:
3516 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3527 3668 4050 5015 6399 6667 1632
Size: 1895 141 382 965 1384 268 1917
Fragments arranged by size:
1917 1895 1384 965 382 268 141
AfeI AGC'GCT
Cuts at: 4225 4225
Size: 6952
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6755
AflIII A'CryG_T
Cuts at: 1203 2155 3501 5851 6828 1203
Size: 952 1346 2350 977 1327
Fragments arranged by size:
2350 1346 1327 977 952
AgeI A'CCGG_T
Cuts at: 5783 5783
Size: 6952
AhdI GACnn_n'nnGTC
Cuts at: 2518 5681 2518
Size: 3163 3789
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3729 1184
Size: 2545 4407
AlwNI CAG_nnn'CTG
Cuts at: 2021 2563 3490 3796 4695 6267 2021
Size: 542 927 306 899 1572 2706
Fragments arranged by size:
2706 1572 927 899 542 306
ApaLI G'TGCA_C
Cuts at: 5009 6165 5009
Size: 1156 5796
ApoI r'AATT_y
Cuts at: 1493 2431 3385 4261 4487 4639 6748 1493
Size: 938 954 876 226 152 2109 1697
Fragments arranged by size:
2109 1697 954 938 876 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2687 5506 160
Size: 872 1655 2819 1606
Fragments arranged by size:
2819 1655 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
AvaI C'yCGr_G
Cuts at: 1870 1943 2460 2985 4139 4277 1870
Size: 73 517 525 1154 138 4545
Fragments arranged by size:
4545 1154 525 517 138 73
BamHI G'GATC_C
Cuts at: 4282 4649 4282
Size: 367 6585
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4273 5728 618
Size: 325 217 627 2486 1455 1842
Fragments arranged by size:
2486 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4271 1391
Size: 2880 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2811 3769 928
Size: 971 912 958 4111
Fragments arranged by size:
4111 971 958 912
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5143 5177 1903
Size: 34 50 34 3122 34 3678
Fragments arranged by size:
3678 3122 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2244 4844 6054 2244
Size: 2600 1210 3142
Fragments arranged by size:
3142 2600 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6952
BfrBI ATG'CAT
Cuts at: 2981 2981
Size: 6952
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5563 136
Size: 107 122 71 631 605 3891 1525
Fragments arranged by size:
3891 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6947 6947
Size: 6952
BlpI GC'TnA_GC
Cuts at: 4100 4369 4100
Size: 269 6683
Bme1580I G_kGCm'C
Cuts at: 1790 2348 2732 5013 6169 1790
Size: 558 384 2281 1156 2573
Fragments arranged by size:
2573 2281 1156 558 384
BmgBI CAC'GTC
Cuts at: 1318 3504 1318
Size: 2186 4766
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2031 2654 3762
Size: 416 53 83 399 24 614 623 1108
Cuts at: 3762 4683 5641 442
Size: 921 958 1753
Fragments arranged by size:
1753 1108 958 921 623 614 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3861 1056
Size: 2805 4147
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2399 2399
Size: 6952
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2292 2418 2540 3722 5612 1239
Size: 1053 126 122 1182 1890 2579
Fragments arranged by size:
2579 1890 1182 1053 126 122
Bpu10I CC'TnA_GC
Cuts at: 2169 3933 2169
Size: 1764 5188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2277 4059 5077 5942 6240 2277
Size: 1782 1018 865 298 2989
Fragments arranged by size:
2989 1782 1018 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2329 5615 882
Size: 617 830 3286 2219
Fragments arranged by size:
3286 2219 830 617
BsaAI yAC'GTr
Cuts at: 493 1204 4743 493
Size: 711 3539 2702
Fragments arranged by size:
3539 2702 711
BsaBI GATnn'nnATC
Cuts at: 3332 6946 3332
Size: 3614 3338
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 3342 4759 5141
Size: 53 83 186 936 834 975 1417 382
Cuts at: 5141 5776 275
Size: 635 1451
Fragments arranged by size:
1451 1417 975 936 834 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5385 5783 6057 6204 6615 5385
Size: 398 274 147 411 5722
Fragments arranged by size:
5722 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4146 4176 1178
Size: 30 165 30 2743 30 3954
Fragments arranged by size:
3954 2743 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6952
BseYI C'CCAG_C
Cuts at: 2470 6155 6651 2470
Size: 3685 496 2771
Fragments arranged by size:
3685 2771 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2162 2195 3555 4240 1163
Size: 573 426 33 1360 685 3875
Fragments arranged by size:
3875 1360 685 573 426 33
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4402 5163 6191 1540
Size: 275 186 2401 761 1028 2301
Fragments arranged by size:
2401 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2992 3084 3698 4188 5013 5098 6169 2992
Size: 92 614 490 825 85 1071 3775
Fragments arranged by size:
3775 1071 825 614 490 92 85
BsmI GAATG_Cn'
Cuts at: 4463 4556 4463
Size: 93 6859
BsmBI CGTCTCn'nnnn_
Cuts at: 2489 2489
Size: 6952
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2348 2732 2992 3084 3698 4188 4271
Size: 399 558 384 260 92 614 490 83
Cuts at: 4271 5013 5098 6169 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 614 558 490 399 384
260 92 85 83
BspEI T'CCGG_A
Cuts at: 6615 6615
Size: 6952
BspHI T'CATG_A
Cuts at: 4841 6571 4841
Size: 1730 5222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2080 2119 2137 4307 844
Size: 1236 39 18 2170 3489
Fragments arranged by size:
3489 2170 1236 39 18
BsrBI CCG'CTC
Cuts at: 1394 1823 2168 4839 1394
Size: 429 345 2671 3507
Fragments arranged by size:
3507 2671 429 345
BsrDI GCAATG_nn'
Cuts at: 65 1248 5447 5621 65
Size: 1183 4199 174 1396
Fragments arranged by size:
4199 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5596 5783 1700
Size: 110 3786 187 2869
Fragments arranged by size:
3786 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2256 2616 96
Size: 2160 360 4432
Fragments arranged by size:
4432 2160 360
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6952
BssSI C'ACGA_G
Cuts at: 3531 5012 6024 3531
Size: 1481 1012 4459
Fragments arranged by size:
4459 1481 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3533 3796 794
Size: 2739 263 3950
Fragments arranged by size:
3950 2739 263
BstBI TT'CG_AA
Cuts at: 3382 5756 3382
Size: 2374 4578
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6952
BstXI CCAn_nnnn'nTGG
Cuts at: 2270 2725 2759 2270
Size: 455 34 6463
Fragments arranged by size:
6463 455 34
BstYI r'GATC_y
Cuts at: 1972 4282 4649 5034 5051 6503 6589 6601 6947
Size: 2310 367 385 17 1452 86 12 346
Cuts at: 6947 1972
Size: 1977
Fragments arranged by size:
2310 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2319 3301 513
Size: 552 1254 982 4164
Fragments arranged by size:
4164 1254 982 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6213
Fragments arranged by size:
6213 683 56
BtsI GCAGTG_nn'
Cuts at: 2085 3059 3543 3713 3856 4030 4242 4468 5262
Size: 974 484 170 143 174 212 226 794
Cuts at: 5262 5282 2085
Size: 20 3755
Fragments arranged by size:
3755 974 794 484 226 212 174 170
143 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6952
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6917
DraI TTT'AAA
Cuts at: 3984 4256 3984
Size: 272 6680
DraIII CAC_nnn'GTG
Cuts at: 2446 2446
Size: 6952
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5959 809
Size: 507 4643 1802
Fragments arranged by size:
4643 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4399 5288 6917
Size: 54 1425 50 275 18 2569 889 1629
Cuts at: 6917 8
Size: 43
Fragments arranged by size:
2569 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4399 1537
Size: 275 2587 4090
Fragments arranged by size:
4090 2587 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2544 3025 3687 4069 4882 1353
Size: 1191 481 662 382 813 3423
Fragments arranged by size:
3423 1191 813 662 481 382
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2293 5539 5913 6059 387
Size: 1064 138 704 3246 374 146 1280
Fragments arranged by size:
3246 1280 1064 704 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2292 2418 2540 3527 3668 3722 4050
Size: 393 660 126 122 987 141 54 328
Cuts at: 4050 5015 5612 6399 6667 1239
Size: 965 597 787 268 1524
Fragments arranged by size:
1524 987 965 787 660 597 393 328
268 141 126 122 54
EcoNI CCTnn'n_nnAGG
Cuts at: 3521 3521
Size: 6952
EcoRI G'AATT_C
Cuts at: 2431 4261 4639 2431
Size: 1830 378 4744
Fragments arranged by size:
4744 1830 378
EcoRV GAT'ATC
Cuts at: 3052 3052
Size: 6952
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3428 3428
Size: 6952
FspI TGC'GCA
Cuts at: 5458 5458
Size: 6952
HaeII r_GCGC'y
Cuts at: 1678 3160 4227 6099 1678
Size: 1482 1067 1872 2531
Fragments arranged by size:
2531 1872 1482 1067
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2021 2041 2053 2370
Size: 32 135 32 437 12 20 12 317
Cuts at: 2370 2402 2504 2536 3041 3073 5593 5625 5667
Size: 32 102 32 505 32 2520 32 42
Cuts at: 5667 5699 1373
Size: 32 2626
Fragments arranged by size:
2626 2520 505 437 317 135 102 42
32 32 32 32 32 32 32 20
12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2912 3230 3592 4296 4542 669
Size: 988 281 974 318 362 704 246 3079
Fragments arranged by size:
3079 988 974 704 362 318 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6952
HpaI GTT'AAC
Cuts at: 4542 4542
Size: 6952
KpnI G_GTAC'C
Cuts at: 4277 4277
Size: 6952
MfeI C'AATT_G
Cuts at: 2478 4551 2478
Size: 2073 4879
MluI A'CGCG_T
Cuts at: 6828 6828
Size: 6952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2007 2547 3200 6066 6250 1305
Size: 702 540 653 2866 184 2007
Fragments arranged by size:
2866 2007 702 653 540 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5130
Fragments arranged by size:
5130 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2757 4910 5269 5428 518
Size: 648 1591 2153 359 159 2042
Fragments arranged by size:
2153 2042 1591 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3467 4368 5045 6193 6438 1606
Size: 1861 901 677 1148 245 2120
Fragments arranged by size:
2120 1861 1148 901 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3301 513
Size: 552 2236 4164
Fragments arranged by size:
4164 2236 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6952
NheI G'CTAG_C
Cuts at: 1052 3857 1052
Size: 2805 4147
NotI GC'GGCC_GC
Cuts at: 4399 4399
Size: 6952
NsiI A_TGCA'T
Cuts at: 2983 2983
Size: 6952
NspI r_CATG'y
Cuts at: 1132 1229 2159 2812 4129 4310 5855 1132
Size: 97 930 653 1317 181 1545 2229
Fragments arranged by size:
2229 1545 1317 930 653 181 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6952
PciI A'CATG_T
Cuts at: 2155 5851 2155
Size: 3696 3256
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2338 3002 3182 3319 1108
Size: 1230 664 180 137 4741
Fragments arranged by size:
4741 1230 664 180 137
PfoI T'CCnGG_A
Cuts at: 3606 3997 3606
Size: 391 6561
PmeI GTTT'AAAC
Cuts at: 4256 4256
Size: 6952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5021 5053 6534 6566 5021
Size: 32 1481 32 5407
Fragments arranged by size:
5407 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6952
PsiI TTA'TAA
Cuts at: 4522 4522
Size: 6952
PstI C_TGCA'G
Cuts at: 830 3066 4249 4304 830
Size: 2236 1183 55 3478
Fragments arranged by size:
3478 2236 1183 55
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6952
SalI G'TCGA_C
Cuts at: 1936 3228 3590 4294 1936
Size: 1292 362 704 4594
Fragments arranged by size:
4594 1292 704 362
SbfI CC_TGCA'GG
Cuts at: 4304 4304
Size: 6952
ScaI AGT'ACT
Cuts at: 4201 5200 6912 4201
Size: 999 1712 4241
Fragments arranged by size:
4241 1712 999
SfcI C'TryA_G
Cuts at: 826 1045 3062 3814 3954 4227 4245 4300 5435
Size: 219 2017 752 140 273 18 55 1135
Cuts at: 5435 6116 6307 826
Size: 681 191 1471
Fragments arranged by size:
2017 1471 1135 752 681 273 219 191
140 55 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6952
SmaI CCC'GGG
Cuts at: 1872 4279 1872
Size: 2407 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2292 2985 4074 4139 5056 5957
Size: 197 926 349 693 1089 65 917 901
Cuts at: 5957 6219 820
Size: 262 1553
Fragments arranged by size:
1553 1089 926 917 901 693 349 262
197 65
SnaBI TAC'GTA
Cuts at: 493 4743 493
Size: 4250 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6952
SphI G_CATG'C
Cuts at: 1132 4129 4310 1132
Size: 2997 181 3774
Fragments arranged by size:
3774 2997 181
SspI AAT'ATT
Cuts at: 5 52 3096 3433 4876 5
Size: 47 3044 337 1443 2081
Fragments arranged by size:
3044 2081 1443 337 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6952
StyI C'CwwG_G
Cuts at: 513 1065 3301 3456 4391 513
Size: 552 2236 155 935 3074
Fragments arranged by size:
3074 2236 935 552 155
SwaI ATTT'AAAT
Cuts at: 3984 3984
Size: 6952
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3613 4979 4996 5149 5334 5779 325
Size: 1255 2033 1366 17 153 185 445 1498
Fragments arranged by size:
2033 1498 1366 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2110 2256 2616
Size: 274 80 33 51 1443 133 146 360
Cuts at: 2616 4199 5198 6910 96
Size: 1583 999 1712 138
Fragments arranged by size:
1712 1583 1443 999 360 274 146 138
133 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6912 6944 6912
Size: 32 6920
Tth111I GACn'n_nGTC
Cuts at: 1316 2023 2637 2660 1316
Size: 707 614 23 5608
Fragments arranged by size:
5608 707 614 23
XbaI T'CTAG_A
Cuts at: 4288 4288
Size: 6952
XhoI C'TCGA_G
Cuts at: 1943 2985 4139 1943
Size: 1042 1154 4756
Fragments arranged by size:
4756 1154 1042
XmaI C'CCGG_G
Cuts at: 1870 4277 1870
Size: 2407 4545
XmnI GAAnn'nnTTC
Cuts at: 5081 5081
Size: 6952
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3343 4760 276
Size: 53 83 186 936 1809 1417 2468
Fragments arranged by size:
2468 1809 1417 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI
BstZ17I Bsu36I EcoICRI EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI XcmI